Standard name
Human Ortholog
Description Cytoplasmic inorganic pyrophosphatase (PPase); homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.96 0.95 0.95 0.95 0.87 0.84 0.91 0.82 0.88 0.88 0.99 0.99 0.97 0.97 0.97 0.98 0.99 0.94 0.94 0.8 0.82 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.12 0.06 0.12 0.07 0.08 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 3 0 1 3 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 2 1 1 2 0 3 0 0 1 4 4 0 0 0 0 0 0 0 2 4 1 4 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 5 8 5 4 8 20 18 14 20 10 15 1 4 0 9 4 3 0 0 0 0 0 0
Cytoplasm 234 497 365 274 200 317 415 399 306 299 306 366 133 179 205 303 207 246 225 464 361 118 133 171
Endoplasmic Reticulum 2 0 4 5 8 9 42 55 20 42 25 35 0 0 2 14 12 13 1 0 0 0 0 1
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Golgi 0 0 0 0 0 0 2 0 3 2 1 1 0 0 0 0 3 1 0 0 0 0 1 0
Mitochondria 0 0 0 0 1 1 0 0 2 1 0 2 0 0 1 4 1 1 0 0 1 3 0 1
Nucleus 0 1 0 1 1 0 2 0 1 0 2 0 1 0 2 1 1 0 0 12 1 11 10 9
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0
Vac/Vac Membrane 0 3 11 3 2 5 6 17 3 12 8 6 0 1 1 2 3 5 0 7 2 4 3 4
Unique Cell Count 235 501 381 287 211 334 478 476 337 365 348 417 134 181 211 313 213 252 228 493 385 147 162 203
Labelled Cell Count 243 508 390 290 218 340 490 491 352 377 358 432 135 184 211 333 231 269 228 493 385 147 162 203


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 64.3 42.6 52.7 52.0 37.8 46.8 45.6 44.8 42.9 44.3 41.4 41.0 65.0 59.3 53.3 64.3 78.2 82.2 49.6 53.9 57.4
Std Deviation (1e-4) 14.3 12.9 14.3 13.0 14.9 13.4 13.1 12.7 12.1 12.5 11.3 11.9 13.7 16.2 15.7 24.1 31.3 29.5 22.1 26.6 24.7
Intensity Change (Log2) – – – -0.02 -0.48 -0.17 -0.21 -0.23 -0.3 -0.25 -0.35 -0.36 0.3 0.17 0.01 0.29 0.57 0.64 -0.09 0.03 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.3 0 0.3 1.7 1.4 1.6 2.4 0.7 1.3 0 0 0 0.7 0 0
Cytoplasm -0.2 -0.6 -0.6 -4.5 -5.6 -2.7 -6.1 -3.9 -4.1 1.9 1.9 0.8 0.7 0.9 1.2
Endoplasmic Reticulum 0 0 1.6 5.0 6.0 3.6 5.9 4.2 4.8 0 0 0 2.8 3.3 3.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.6 0 -1.3 -1.7 0.6 -1.9 0.3 -0.5 -1.4 0 0 0 -2.2 -1.1 -0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0199 0.0001 0.0196 0.0034 0.005 0.0001 0.0003 0.0001 0.0004 0.0001 0 0 0.0153 0.0002 0.0005 0 0.0102 0
Bud 0.0026 0.0001 0.0003 0.0007 0.0009 0.0001 0.0001 0 0.0011 0 0 0 0.0002 0.0001 0.0001 0 0.0001 0
Bud Neck 0.001 0.0001 0.0003 0.0003 0.0008 0.0002 0.0002 0 0.0001 0.0004 0.0002 0.0002 0.0003 0.0001 0.0002 0.0002 0.0007 0.0006
Bud Periphery 0.0013 0 0.0002 0.0013 0.0004 0 0 0 0.0002 0 0 0 0.0002 0 0.0001 0 0.0001 0
Bud Site 0.0034 0 0.0008 0.001 0.0003 0.0001 0.0001 0 0.0002 0 0 0 0.0006 0.0001 0.0001 0 0.0006 0
Cell Periphery 0.0004 0 0.0001 0.0003 0.0002 0 0.0002 0 0 0 0 0 0.0003 0.0001 0 0 0 0
Cytoplasm 0.8403 0.9981 0.9382 0.873 0.8836 0.9538 0.9929 0.9988 0.9195 0.9962 0.9977 0.9971 0.8858 0.9971 0.9791 0.998 0.9534 0.9957
Cytoplasmic Foci 0.0134 0.0002 0.0116 0.0077 0.0136 0.0005 0.001 0.0001 0.002 0.0001 0 0.0005 0.0246 0.0003 0.0027 0.0001 0.0051 0.0001
Eisosomes 0.0001 0 0 0.0001 0.0002 0 0 0 0 0 0 0 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0065 0 0.0005 0.004 0.0006 0.0002 0.0004 0 0.0035 0.0001 0 0 0.0019 0.0001 0.0003 0 0.0001 0.0001
Endosome 0.0271 0 0.0054 0.0233 0.0098 0.001 0.0003 0 0.0178 0 0 0.0001 0.0099 0 0.0005 0 0.0027 0
Golgi 0.0076 0 0.0057 0.0095 0.0056 0 0 0 0.0001 0 0 0 0.004 0 0.0001 0 0.0065 0
Lipid Particles 0.0089 0 0.0029 0.0078 0.0377 0.0001 0 0 0.0001 0 0 0 0.0144 0 0.0021 0 0.004 0
Mitochondria 0.0092 0.0001 0.001 0.052 0.0114 0.0003 0 0 0.0003 0 0 0 0.004 0 0.0009 0 0.0017 0
None 0.0159 0.0002 0.0015 0.0029 0.0039 0.0003 0.002 0 0.0009 0.0001 0 0.0001 0.0137 0.0003 0.0007 0.0001 0.0002 0.0001
Nuclear Periphery 0.0104 0.0002 0.0007 0.0027 0.0008 0.0017 0.0005 0.0001 0.0293 0.0003 0.0001 0.0001 0.0032 0.0002 0.0009 0.0001 0.0004 0.0005
Nucleolus 0.0017 0 0.0004 0.0002 0.0004 0.0065 0 0 0 0 0 0 0.0026 0 0.0003 0 0 0
Nucleus 0.0119 0.0008 0.0021 0.0025 0.0021 0.0307 0.0008 0.0008 0.0048 0.0025 0.002 0.0016 0.0059 0.001 0.0034 0.0014 0.0022 0.0027
Peroxisomes 0.0029 0 0.0035 0.0019 0.0095 0 0 0 0 0 0 0 0.0043 0 0.0024 0 0.0117 0
Punctate Nuclear 0.0035 0 0.0005 0.0005 0.0009 0.0006 0.0001 0 0.0002 0 0 0 0.0064 0.0001 0.0016 0 0.0002 0
Vacuole 0.0084 0.0001 0.0036 0.0026 0.0109 0.0028 0.001 0 0.0115 0 0 0.0001 0.0014 0.0002 0.0028 0 0.0001 0.0001
Vacuole Periphery 0.0034 0 0.0012 0.0023 0.0015 0.001 0.0001 0 0.0077 0 0 0 0.0008 0 0.0012 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1049.1282 948.7021 837.8269 942.6638 851.9096 947.0751 1006.2698 976.9682 893.0566 930.5201
Translational Efficiency 1.7363 1.7484 1.7677 1.3788 1.6824 1.9304 1.7422 1.6127 1.9201 1.7807

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
555 1234 2416 1792 2207 167 2252 1879 2762 1401 4668 3671

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3315.38 4374.97 3662.72 4848.22 3305.91 4598.26 4892.37 5067.30 3307.81 4401.59 4255.94 4960.36
Standard Deviation 594.88 1086.80 1481.78 1575.82 764.19 735.94 1271.84 1656.00 733.32 1053.63 1514.70 1621.06
Intensity Change Log 2 — 0.400099 0.143741 0.548281 — 0.476041 0.565486 0.616170 — 0.438515 0.369671 0.582576

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002167 0.000538 0.034124 0.002323 0.000525 0.010614 0.001902 0.003131 0.000855 0.001739 0.018579 0.002737
Bud Neck 0.004972 0.001640 0.015504 0.004632 0.002329 0.008221 0.004385 0.003862 0.002860 0.002425 0.010140 0.004238
Bud Site 0.052691 0.013489 0.199273* 0.047475 0.022408 0.029007 0.041279 0.048333 0.028493 0.015339 0.123051* 0.047914
Cell Periphery 0.009333 0.002904 0.003092 0.001846 0.001167 0.010096 0.003854 0.003717 0.002808 0.003761 0.003460 0.002804
Cytoplasm 0.769911* 0.833904* 0.580753* 0.799540* 0.888639* 0.678930* 0.823879* 0.832770* 0.864781* 0.815431* 0.698045* 0.816549*
Cytoplasmic Foci 0.034678 0.020911 0.045224 0.009909 0.010242 0.066147 0.011477 0.009237 0.015152 0.026303 0.028943 0.009565
Eisosomes 0.000015 0.000003 0.000053 0.000008 0.000004 0.000020 0.000009 0.000012 0.000006 0.000005 0.000032 0.000010
Endoplasmic Reticulum 0.000397 0.000064 0.000303 0.000231 0.000153 0.000203 0.000082 0.000125 0.000202 0.000080 0.000196 0.000176
Endosome 0.000790 0.000338 0.003621 0.001294 0.000337 0.000755 0.000346 0.000245 0.000428 0.000387 0.002041 0.000757
Golgi 0.011189 0.004806 0.004260 0.001001 0.003015 0.009818 0.001163 0.000380 0.004658 0.005404 0.002766 0.000683
Lipid Particles 0.027167 0.007933 0.028372 0.003252 0.006954 0.029347 0.011139 0.004077 0.011015 0.010486 0.020058 0.003674
Mitochondria 0.000071 0.000127 0.000451 0.001505 0.000152 0.000080 0.000169 0.000279 0.000135 0.000121 0.000315 0.000877
Mitotic Spindle 0.002294 0.001899 0.011654 0.004653 0.000631 0.003517 0.000861 0.000926 0.000965 0.002092 0.006447 0.002745
None 0.007061 0.010731 0.001806 0.004323 0.007899 0.003806 0.003939 0.004377 0.007730 0.009906 0.002835 0.004351
Nuclear Periphery 0.000173 0.000147 0.000102 0.000111 0.000049 0.000436 0.000106 0.000079 0.000074 0.000181 0.000104 0.000095
Nuclear Periphery Foci 0.011156 0.004525 0.001886 0.001133 0.002730 0.013611 0.002221 0.001359 0.004423 0.005608 0.002048 0.001249
Nucleolus 0.000070 0.000084 0.000122 0.000276 0.000113 0.000082 0.000138 0.000129 0.000104 0.000084 0.000130 0.000201
Nucleus 0.037160 0.080939 0.021451 0.038555 0.041594 0.089005 0.046018 0.042659 0.040703 0.081900 0.033303 0.040656
Peroxisomes 0.000453 0.000157 0.009662 0.001025 0.000433 0.000354 0.000581 0.000620 0.000437 0.000180 0.005281 0.000818
Vacuole 0.025361 0.013746 0.037618 0.074967 0.009535 0.045298 0.045904 0.043368 0.012715 0.017507 0.041615 0.058793
Vacuole Periphery 0.002891 0.001115 0.000669 0.001939 0.001094 0.000651 0.000551 0.000315 0.001455 0.001060 0.000612 0.001108

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.23 -13.07 1.42 -1.43 13.32 -1.68 -5.25 -2.29 1.34 0.45 -0.97 -14.17 -1.83 -0.35 12.59
Bud Neck 5.12 -14.32 -1.00 -9.41 16.14 -4.30 -8.88 -4.97 2.94 3.82 0.15 -20.07 -6.42 -6.23 15.56
Bud Site 5.95 -17.59 2.67 -6.60 28.99* -1.29 -6.09 -5.53 -1.55 0.26 4.78 -25.03 -3.38 -8.80 22.92*
Cell Periphery 5.95 5.96 7.40 5.03 5.84 -5.77 -9.25 -6.12 4.09 0.75 -2.99 -2.35 0.64 3.69 3.28
Cytoplasm -4.94 16.98 1.78 10.20 -21.83 12.06 19.20 15.85 -7.18 -2.85 9.24 35.49 18.13 5.08 -18.66
Cytoplasmic Foci 3.52 -3.25 7.22 5.21 18.40 -7.55 -4.09 -0.95 7.42 3.37 -5.87 -11.83 3.61 8.16 17.22
Eisosomes 6.49 -10.96 3.46 -5.56 15.02 -3.71 -9.06 -6.97 2.08 0.31 0.29 -16.82 -4.98 -4.45 13.45
Endoplasmic Reticulum 3.99 0.76 2.74 -1.34 3.61 -1.11 3.75 2.07 1.82 -0.68 5.11 -0.31 2.61 -1.11 3.25
Endosome 2.81 -8.59 -0.25 -2.56 7.93 -3.10 -0.82 2.22 3.93 3.02 0.27 -9.96 -1.03 -1.01 8.44
Golgi 4.46 5.71 8.41 6.34 7.25 -4.02 7.76 11.99 6.00 6.36 -0.98 5.96 13.12 8.09 8.73
Lipid Particles 5.79 -1.85 6.90 1.87 14.09 -4.22 -5.37 0.85 4.41 7.15 0.03 -8.98 4.80 3.85 15.65
Mitochondria -3.25 -14.01 -2.10 -1.92 -0.90 4.30 -2.40 -2.61 -4.36 -1.43 0.69 -10.75 -2.27 -2.34 -1.03
Mitotic Spindle 0.19 -8.08 -1.84 -1.94 6.11 -1.55 -2.39 -0.84 1.34 1.33 -2.54 -9.70 -3.43 -0.22 6.41
None -3.00 5.71 4.09 8.52 -3.14 5.85 6.54 5.85 -0.16 -0.16 -2.22 9.31 7.44 8.35 -2.37
Nuclear Periphery 0.96 -0.42 -1.02 -2.19 -0.77 -8.60 -12.02 -11.12 6.74 3.46 -9.58 -13.54 -11.84 2.28 1.80
Nuclear Periphery Foci 6.72 9.83 10.97 5.93 3.61 -6.95 -0.56 2.37 7.52 4.47 -2.64 4.92 7.97 8.43 5.64
Nucleolus -2.16 -8.82 -8.36 -7.39 -4.89 1.34 0.56 0.83 -4.29 1.90 1.26 0.31 -0.41 -8.00 -3.52
Nucleus -11.61 2.45 -6.27 6.61 -10.66 -7.42 -8.83 -7.36 5.16 1.24 -14.85 -3.35 -9.69 8.58 -6.84
Peroxisomes 2.40 -10.50 -0.45 -1.60 9.74 0.86 -0.01 0.00 -0.73 0.01 3.23 -10.23 -0.41 -2.22 9.48
Vacuole 3.91 -10.72 -20.70 -25.72 -13.52 -7.43 -24.05 -21.73 -1.79 -0.67 -4.34 -29.47 -33.02 -26.46 -10.64
Vacuole Periphery 1.27 1.65 1.28 0.05 -1.16 2.02 2.60 3.60 2.92 4.06 0.99 2.71 2.16 1.16 -0.47
Standard name
Human Ortholog
Description Cytoplasmic inorganic pyrophosphatase (PPase); homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Ipp1

Ipp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ipp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available