Standard name
Human Ortholog
Description Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0 – 0.07 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0.12 0.1 0 0 0 0 0 0 0
Cytoplasm 0 0.18 0.21 0.22 0.21 0.15 0.16 – 0.14 0.19 0.2 0.19 0 0 0.05 0.08 0.06 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.26 0 0.16 0.15 0.24 0 0.19 – 0 0.06 0.05 0 0.06 0.07 0.12 0.19 0.33 0.1 0.15 0.12 0.07 0.1 0.09 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0.14 0.17 0.17 0.3 0.15 0.43 0 0 0 0 0 0
Mitochondria 0.63 0.83 0.41 0.59 0.42 0.79 0.57 – 0.78 0.76 0.8 0.74 0.8 0.79 0.67 0.12 0.16 0.14 0.59 0.63 0.75 0.71 0.69 0.69
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0.12 0 0.09 0.19 0.13 0 0.06 – 0 0 0 0 0 0 0.12 0.1 0.15 0.14 0 0 0.05 0 0 0
SpindlePole 0.08 0 0.21 0.09 0.11 0.06 0.14 – 0.12 0.09 0.07 0.1 0 0 0 0.06 0.12 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0.06 0.19 0.06 0.1 – 0.05 0 0 0 0.11 0.09 0.08 0.16 0.24 0.12 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 3 0 1 2 0 – 0 0 0 0 2 3 2 3 0 1 1 0 2 4 5 9
Bud 0 3 1 1 4 10 9 – 14 2 11 20 1 4 5 1 1 1 0 0 0 0 5 1
Bud Neck 0 0 2 1 1 4 0 – 7 2 2 5 0 0 0 0 0 0 0 0 1 1 3 6
Bud Site 2 0 0 0 0 1 0 – 1 2 3 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 7 2 0 1 – 0 1 2 6 2 10 6 10 7 1 0 0 0 0 0 0
Cytoplasm 2 16 20 31 52 31 55 – 30 48 48 64 4 12 11 7 4 4 1 4 1 1 3 4
Endoplasmic Reticulum 0 4 2 0 1 3 1 – 0 1 0 1 6 4 4 4 3 1 0 1 2 1 0 1
Endosome 28 0 15 21 59 3 66 – 3 16 11 9 7 20 28 16 22 4 22 12 12 17 23 15
Golgi 1 0 0 0 1 0 0 – 0 0 0 0 16 49 40 25 10 18 6 2 2 2 5 4
Mitochondria 67 75 39 84 102 162 199 – 163 194 191 249 93 228 161 10 11 6 86 64 130 117 175 167
Nucleus 3 2 1 1 3 8 14 – 3 4 6 8 0 1 0 3 1 0 0 1 0 0 0 1
Nuclear Periphery 3 0 0 0 0 0 0 – 0 0 1 2 0 1 1 3 0 1 4 0 1 2 0 1
Nucleolus 10 0 0 0 0 3 3 – 0 0 0 1 0 1 0 1 2 2 1 0 1 0 1 1
Peroxisomes 13 0 9 27 32 1 21 – 2 8 4 8 2 10 29 8 10 6 5 1 9 5 11 6
SpindlePole 8 0 20 13 27 12 49 – 25 22 16 33 4 4 5 5 8 5 5 3 2 2 6 10
Vac/Vac Membrane 6 1 3 8 47 13 36 – 10 7 6 8 13 26 20 13 16 5 6 8 3 4 7 6
Unique Cell Count 106 90 95 142 244 205 347 208 256 240 335 116 287 241 83 67 42 147 102 173 165 253 242
Labelled Cell Count 143 104 117 194 332 253 454 258 307 301 419 150 373 312 109 95 55 147 102 173 165 253 242


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.9 5.1 5.3 5.8 3.9 4.6 – 3.9 4.4 4.0 4.0 6.2 6.1 6.5 7.6 8.2 8.2 4.6 4.8 4.7
Std Deviation (1e-4) 0.8 0.6 1.2 1.8 1.4 1.4 1.3 – 1.3 1.6 1.3 1.1 1.4 1.2 1.4 1.3 1.8 2.0 1.1 1.5 0.9
Intensity Change (Log2) – – – 0.07 0.17 -0.38 -0.15 – -0.38 -0.22 -0.34 -0.34 0.27 0.27 0.35 0.58 0.68 0.69 -0.13 -0.09 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 2.9 4.0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1036 0.5231 0.259 0.0484 0.1301 0.2605 0.623 1.6938 1.3502 1.3008 0.8633 1.2987 2.583 3.4214 2.9679 3.071 2.4847 3.0662
Actin 0.0104 0.0081 0.0058 0.0014 0.0072 0.0027 0.0186 0.0031 0.008 0.0019 0.0022 0.0032 0.0141 0.0056 0.0212 0.0269 0.0475 0.0111
Bud 0.0008 0.0017 0.0018 0.0008 0.0005 0.0013 0.0012 0.0011 0.0022 0.001 0.0006 0.0004 0.0009 0.0057 0.002 0.0014 0.0014 0.0006
Bud Neck 0.0043 0.0012 0.0011 0.0011 0.0005 0.0021 0.0008 0.0034 0.0009 0.0004 0.0005 0.0014 0.005 0.0011 0.0012 0.0037 0.0005 0.0018
Bud Periphery 0.0022 0.0055 0.0054 0.0019 0.0008 0.0056 0.0014 0.0014 0.0065 0.0026 0.0017 0.0009 0.0018 0.0091 0.0064 0.0045 0.0037 0.0013
Bud Site 0.0059 0.0166 0.0107 0.0008 0.0009 0.0011 0.0033 0.0154 0.009 0.0007 0.0006 0.0007 0.0111 0.0307 0.0075 0.0044 0.0014 0.0012
Cell Periphery 0.0012 0.0006 0.0036 0.0003 0.0001 0.0015 0.0004 0.0004 0.0007 0.0003 0.0007 0.0003 0.0015 0.0008 0.0009 0.0005 0.0003 0.0003
Cytoplasm 0.0132 0.0097 0.0084 0.0073 0.0012 0.0166 0.0148 0.0139 0.0044 0.0031 0.0023 0.004 0.0207 0.0218 0.0233 0.0118 0.0036 0.0139
Cytoplasmic Foci 0.0612 0.0918 0.0741 0.058 0.0247 0.0812 0.0672 0.0581 0.0394 0.0435 0.0443 0.0324 0.0597 0.0698 0.0551 0.0568 0.054 0.0624
Eisosomes 0.0021 0.0003 0.001 0.0001 0.0001 0.0002 0.0004 0.0001 0.0002 0.0002 0.0003 0.0002 0.001 0.0004 0.0007 0.0008 0.0007 0.0003
Endoplasmic Reticulum 0.003 0.0014 0.0009 0.0004 0.0014 0.0011 0.0028 0.0003 0.0006 0.0005 0.0005 0.001 0.0077 0.0025 0.0038 0.0038 0.0026 0.0009
Endosome 0.1447 0.11 0.0965 0.1197 0.048 0.1172 0.1617 0.082 0.0739 0.1357 0.0827 0.074 0.1372 0.1149 0.0761 0.0848 0.0946 0.0874
Golgi 0.0599 0.098 0.0625 0.0694 0.0358 0.0676 0.0681 0.0752 0.0669 0.0665 0.1156 0.0625 0.0619 0.0954 0.0489 0.0836 0.1073 0.0731
Lipid Particles 0.058 0.0157 0.0377 0.0118 0.0087 0.0332 0.0458 0.0238 0.0151 0.0062 0.0087 0.0179 0.0705 0.0148 0.023 0.0271 0.0149 0.0303
Mitochondria 0.4751 0.5333 0.5439 0.6694 0.772 0.5331 0.5318 0.6477 0.7055 0.6506 0.6276 0.7282 0.4786 0.5179 0.6188 0.5311 0.5667 0.5635
None 0.052 0.0317 0.0438 0.0009 0.048 0.0315 0.0093 0.003 0.0025 0.0014 0.0003 0.0124 0.029 0.029 0.0258 0.0576 0.0054 0.0368
Nuclear Periphery 0.0033 0.0005 0.0004 0.0002 0.0049 0.0006 0.0016 0.0001 0.0004 0.0003 0.0006 0.0004 0.004 0.0004 0.0021 0.0005 0.0081 0.0011
Nucleolus 0.0079 0.0051 0.002 0.0002 0.0003 0.0005 0.0008 0.0005 0.0022 0.0002 0.0001 0.0004 0.0052 0.0007 0.0018 0.0004 0.0003 0.0006
Nucleus 0.0023 0.0014 0.0009 0.0003 0.0009 0.0006 0.0021 0.0004 0.001 0.0004 0.0001 0.0005 0.0032 0.0006 0.0013 0.0003 0.0007 0.0005
Peroxisomes 0.0636 0.0586 0.0848 0.0487 0.037 0.0902 0.0475 0.0628 0.0483 0.0735 0.0984 0.0497 0.0667 0.0708 0.0688 0.0904 0.0778 0.1041
Punctate Nuclear 0.0041 0.0013 0.0012 0.0002 0.0006 0.0011 0.0019 0.0009 0.0003 0.0003 0.0001 0.0004 0.0077 0.0004 0.0016 0.0009 0.002 0.0008
Vacuole 0.017 0.0051 0.0103 0.0041 0.0024 0.0071 0.0121 0.0039 0.0081 0.006 0.0041 0.0046 0.0083 0.0052 0.0063 0.0049 0.0029 0.0043
Vacuole Periphery 0.0078 0.0023 0.0033 0.0029 0.0039 0.0037 0.0062 0.0023 0.0038 0.0048 0.0079 0.0043 0.0043 0.0024 0.0033 0.0037 0.0037 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.6285 9.3407 19.4073 13.2363 4.0642 25.0635 41.0829 37.6084 26.9514 27.6647
Translational Efficiency 2.6061 3.8702 1.1958 1.4377 3.0253 2.758 1.7776 1.6252 2.0794 1.8039

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1508 255 111 43 1525 2411 239 1418 3033 2666 350 1461

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 752.27 923.61 972.53 846.33 743.09 835.27 885.96 822.29 747.65 843.72 913.42 823.00
Standard Deviation 96.41 148.04 97.87 105.37 102.91 115.35 90.44 98.04 99.84 121.68 101.23 98.35
Intensity Change Log 2 — 0.296033 0.370492 0.169970 — 0.168706 0.253705 0.146110 — 0.234164 0.313638 0.158163

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000593 0.008056 0.007144 0.006398 0.000695 0.000887 0.000287 0.000442 0.000644 0.001573 0.002462 0.000618
Bud Neck 0.007079 0.006237 0.011754 0.007078 0.005845 0.006977 0.002655 0.007964 0.006458 0.006906 0.005541 0.007938
Bud Site 0.011114 0.025203 0.038224 0.029267 0.008717 0.012699 0.002894 0.024805 0.009908 0.013895 0.014099 0.024936
Cell Periphery 0.000535 0.000605 0.000477 0.000317 0.000449 0.000275 0.000190 0.000153 0.000492 0.000307 0.000281 0.000158
Cytoplasm 0.005718 0.011436 0.239272* 0.301922* 0.004569 0.005190 0.340903* 0.544917* 0.005140 0.005788 0.308672* 0.537765*
Cytoplasmic Foci 0.030561 0.049348 0.127068 0.135820 0.038119 0.047048 0.055753 0.058089 0.034361 0.047268 0.078370 0.060377
Eisosomes 0.000088 0.000114 0.000235 0.000155 0.000064 0.000078 0.000098 0.000063 0.000076 0.000081 0.000141 0.000065
Endoplasmic Reticulum 0.000377 0.000675 0.014673 0.003459 0.000524 0.000209 0.002689 0.001265 0.000451 0.000253 0.006490 0.001330
Endosome 0.070646 0.079597 0.061574 0.020058 0.080553 0.100296 0.007814 0.008623 0.075627 0.098316 0.024863 0.008960
Golgi 0.081571 0.093581 0.002835 0.002525 0.092333 0.133618* 0.000124 0.001777 0.086983 0.129789* 0.000984 0.001799
Lipid Particles 0.002550 0.002603 0.016163 0.011919 0.005313 0.002612 0.004066 0.001779 0.003939 0.002611 0.007902 0.002077
Mitochondria 0.728260* 0.595926* 0.001453 0.001567 0.692104* 0.608854* 0.000446 0.001508 0.710081* 0.607617* 0.000766 0.001509
Mitotic Spindle 0.006332 0.015790 0.022271 0.027193 0.009837 0.012557 0.001541 0.014266 0.008094 0.012866 0.008115 0.014646
None 0.000417 0.000241 0.001265 0.001658 0.000486 0.000362 0.005766 0.003676 0.000452 0.000350 0.004339 0.003617
Nuclear Periphery 0.000116 0.000118 0.002321 0.000813 0.000197 0.000124 0.001721 0.001040 0.000157 0.000124 0.001911 0.001033
Nuclear Periphery Foci 0.000141 0.000456 0.011556 0.002402 0.000288 0.000254 0.002129 0.000939 0.000215 0.000274 0.005119 0.000982
Nucleolus 0.000298 0.000163 0.004426 0.000899 0.000480 0.000725 0.001430 0.000770 0.000390 0.000672 0.002380 0.000774
Nucleus 0.000943 0.003390 0.200980* 0.161075* 0.001434 0.000803 0.475719* 0.206282* 0.001190 0.001051 0.388588* 0.204951*
Peroxisomes 0.021051 0.091643 0.033967 0.080267 0.028834 0.042258 0.001494 0.004584 0.024964 0.046982 0.011792 0.006811
Vacuole 0.013731 0.008215 0.199564 0.203762* 0.011658 0.013768 0.092011 0.115139 0.012689 0.013237 0.126121 0.117747
Vacuole Periphery 0.017881 0.006603 0.002779 0.001445 0.017502 0.010405 0.000269 0.001920 0.017690 0.010041 0.001065 0.001906

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.14 -5.25 -1.84 0.95 0.94 -1.04 2.44 2.18 3.43 -0.50 -3.36 -4.19 0.99 3.83 4.48
Bud Neck 0.37 -1.85 0.48 0.13 1.85 -0.86 2.48 -1.47 -0.70 -5.26 -0.41 0.83 -1.14 -0.76 -2.04
Bud Site -2.40 -4.48 -1.49 -0.12 0.91 -2.76 4.39 -4.13 -2.46 -6.65 -3.00 -1.87 -3.95 -2.07 -1.58
Cell Periphery -0.41 0.30 2.08 2.34 2.89 2.06 3.08 3.85 7.21 2.84 2.54 2.78 5.07 7.69 5.96
Cytoplasm -1.59 -9.61 -6.12 -5.92 -0.84 -0.56 -19.36 -64.69* -64.79* -10.13 -0.81 -21.16 -64.34 -64.15 -13.19
Cytoplasmic Foci -3.51 -7.62 -3.55 -2.53 0.64 -3.73 -1.92 -4.28 -1.47 -0.45 -6.65 -6.64 -6.61 -1.91 3.06
Eisosomes -1.79 -5.79 -3.45 -2.27 2.58 -2.85 -4.77 -0.12 2.34 4.47 -0.84 -6.40 1.29 2.52 7.43
Endoplasmic Reticulum -1.67 -8.42 -4.79 -4.23 5.88 3.04 -8.90 -8.14 -16.86 6.10 3.20 -10.23 -12.19 -16.47 8.48
Endosome -1.08 1.46 9.99 7.50 4.06 -4.20 19.42 20.07 29.77 0.85 -6.01 12.86 26.43 30.99 4.88
Golgi -1.16 22.00 22.06 9.82 0.99 -7.68 23.79 23.45 35.45* -3.08 -9.78 32.64 32.37 36.70 -0.76
Lipid Particles -0.11 -6.06 -1.69 -1.63 1.78 2.75 1.16 3.98 1.91 5.49 2.07 -4.32 3.75 1.48 7.19
Mitochondria 5.26 92.14* 91.96* 24.20* 0.31 7.51 80.97* 80.87* 86.09* -3.08 11.48 121.78* 121.66* 89.17* -1.92
Mitotic Spindle -2.44 -2.06 -0.69 -0.11 0.16 -1.80 5.07 -1.81 -0.60 -5.86 -4.23 0.15 -2.99 -0.50 -2.09
None 1.97 -3.90 -2.50 -2.85 -0.86 0.68 -5.48 -7.06 -7.66 2.00 0.77 -5.88 -7.64 -7.83 0.93
Nuclear Periphery -0.26 -6.80 -5.38 -5.25 3.54 0.84 -10.67 -11.67 -11.74 3.73 0.46 -12.78 -13.42 -12.22 4.46
Nuclear Periphery Foci -1.88 -5.02 -3.90 -3.24 3.89 0.27 -6.37 -3.31 -3.25 2.76 -0.64 -6.45 -3.78 -3.42 4.88
Nucleolus 1.07 -2.14 -2.11 -3.27 1.98 -0.84 -3.17 -1.76 -0.15 2.12 -1.11 -2.87 -2.89 -0.35 2.35
Nucleus -1.42 -8.23 -5.28 -5.18 0.36 0.98 -25.56 -38.22 -38.50 12.57 0.20 -23.81 -38.71 -38.74 9.28
Peroxisomes -5.79 -1.60 -1.14 1.39 -0.70 -4.00 11.76 10.70 15.32 -2.06 -7.53 5.16 11.00 15.56 2.54
Vacuole 2.60 -10.71 -6.03 -6.14 -1.46 -1.74 -10.42 -28.68 -28.26 -2.54 -0.60 -14.44 -29.07 -28.84 -0.70
Vacuole Periphery 6.65 12.20 13.22 4.31 2.27 5.57 15.40 13.82 12.90 -2.71 7.71 20.18 18.51 13.23 -0.77
Standard name
Human Ortholog
Description Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Fmp23

Fmp23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmp23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available