Standard name
Human Ortholog
Description 2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively; yeast null mutant can be functionally complemented by human FTSJ1, mutations in which have been implicated in nonsyndromic X-linked intellectual disability (NSXLID)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.14 0.13 0 0 0 0 0 0
Cytoplasm 0.98 1.0 – 0.94 0.97 0.98 0.89 0.83 0.75 0.63 0.64 0.65 0.95 0.99 0.99 0.93 0.94 0.93 0.92 0.93 0.78 0.93 0.93 0.93
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0.12 0 0 0.22 0.24 0.46 0.53 0.62 0.45 0 0 0 0 0 0 0 0 0.13 0 0 0
Nucleus 0 0 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 1 1 0 1 0 1 0 1 0 2 0 0 1 0 0 1 0 1
Bud 0 2 – 0 0 0 5 10 3 3 4 6 2 1 0 1 0 0 2 0 0 0 1 5
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 – 0 4 4 2 12 0 2 3 3 10 13 19 15 19 35 0 0 0 0 0 1
Cytoplasm 166 76 – 16 224 224 267 233 92 74 90 124 400 626 646 286 132 251 157 77 17 206 375 472
Endoplasmic Reticulum 0 0 – 0 1 1 1 0 1 2 2 3 7 0 2 8 3 11 0 0 0 5 6 6
Endosome 0 0 – 0 0 0 1 2 0 1 0 0 3 1 0 0 0 0 1 0 0 0 0 4
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 1 0 4 0 0 3 2 0 0 1 2 3
Mitochondria 3 1 – 2 7 7 67 67 56 62 87 86 1 0 1 4 3 2 2 1 2 1 4 5
Nucleus 0 0 – 1 0 0 0 2 3 1 3 3 0 0 0 7 1 4 0 0 0 0 1 0
Nuclear Periphery 0 1 – 0 0 0 0 1 0 0 0 1 1 0 0 0 1 1 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 0 1
Vac/Vac Membrane 0 0 – 0 4 2 5 8 0 2 1 7 11 5 2 13 2 3 1 2 0 1 5 2
Unique Cell Count 170 76 17 230 229 299 282 123 118 141 192 419 631 653 309 140 270 172 83 22 221 404 510
Labelled Cell Count 171 83 19 240 238 349 336 155 148 190 234 437 647 675 336 161 310 172 83 22 221 404 510


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 5.5 – 5.6 7.1 7.1 5.2 5.7 4.6 4.8 4.6 5.0 8.9 8.8 8.3 11.2 13.5 12.9 9.1 8.3 8.9
Std Deviation (1e-4) 1.2 1.5 – 0.7 1.4 1.4 1.4 1.5 0.9 1.0 1.0 1.1 1.6 1.6 1.6 2.5 3.3 2.9 4.3 1.5 1.8
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.211 1.8082 1.3624 1.0343 2.0748 1.7035 0.8994 2.6481 2.2409 2.0959 1.4373 1.7692 2.4467 2.2301 1.538 2.0255 1.2419 1.6613
Actin 0.0362 0.0022 0.01 0.0763 0.058 0.0052 0.0033 0.0002 0.0014 0.0002 0 0.0016 0.0077 0.0005 0.0167 0.0343 0.0155 0.0025
Bud 0.0013 0.0014 0.0021 0.0065 0.0006 0.0006 0.0038 0.0004 0.0046 0.0002 0.0001 0.0013 0.0026 0.003 0.0044 0.0009 0.0029 0.0004
Bud Neck 0.0064 0.0003 0.0088 0.0008 0.0035 0.0021 0.0008 0.0002 0.0005 0.0002 0.0001 0.0013 0.005 0.0002 0.0004 0.0024 0.001 0.0009
Bud Periphery 0.0016 0.0014 0.0036 0.0214 0.0007 0.0006 0.0073 0.0002 0.0019 0.0001 0 0.0017 0.001 0.0012 0.0042 0.0013 0.0019 0.0006
Bud Site 0.0171 0.0101 0.0092 0.0012 0.0012 0.0014 0.0033 0.0027 0.0039 0.0005 0 0.0056 0.0064 0.0224 0.0015 0.0046 0.0336 0.0004
Cell Periphery 0.0016 0.0004 0.0003 0.0002 0.0002 0.0001 0.0004 0.0002 0.0002 0.0001 0 0.0006 0.0008 0.0003 0.0002 0.0006 0.0004 0.0001
Cytoplasm 0.429 0.7059 0.5828 0.5296 0.3445 0.692 0.5499 0.8117 0.7172 0.7346 0.6834 0.826 0.6877 0.8492 0.7953 0.7165 0.602 0.8309
Cytoplasmic Foci 0.051 0.0167 0.0216 0.0306 0.066 0.0303 0.0436 0.0098 0.027 0.0424 0.0084 0.0104 0.0226 0.011 0.0213 0.0187 0.0488 0.023
Eisosomes 0.0006 0.0002 0.0001 0.0003 0.0008 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0.0003 0
Endoplasmic Reticulum 0.0069 0.008 0.0066 0.0054 0.0059 0.0074 0.005 0.0016 0.0035 0.0034 0.0014 0.0049 0.0105 0.0022 0.0042 0.005 0.003 0.0049
Endosome 0.0688 0.0091 0.0258 0.027 0.0282 0.0257 0.0576 0.0086 0.0232 0.0668 0.0059 0.0128 0.0224 0.0107 0.0205 0.0391 0.0123 0.0347
Golgi 0.0133 0.0008 0.0029 0.0092 0.0092 0.0032 0.0109 0.0016 0.0047 0.0087 0.0002 0.0013 0.0048 0.0007 0.0067 0.0078 0.0099 0.005
Lipid Particles 0.0132 0.0003 0.0009 0.0019 0.0074 0.0062 0.0087 0.0002 0.0034 0.0203 0.0001 0.0004 0.0047 0.0002 0.0019 0.0066 0.0189 0.0023
Mitochondria 0.0071 0.0007 0.0067 0.0038 0.0036 0.002 0.0053 0.0004 0.008 0.0135 0.0002 0.0134 0.0116 0.0004 0.009 0.0095 0.0445 0.0117
None 0.2894 0.2286 0.3016 0.2523 0.3657 0.1845 0.2744 0.1574 0.1796 0.0968 0.2969 0.1005 0.1789 0.0916 0.105 0.1004 0.1436 0.06
Nuclear Periphery 0.0065 0.0007 0.0037 0.0042 0.0479 0.0079 0.0015 0.0004 0.0017 0.0007 0.0004 0.0116 0.0011 0.0004 0.0008 0.0022 0.0015 0.0041
Nucleolus 0.0118 0.0001 0.0004 0.0003 0.0008 0.0012 0.0003 0.0001 0.0006 0.0001 0 0.0001 0.0122 0.0001 0.0001 0.0003 0.0008 0.0003
Nucleus 0.0056 0.007 0.0025 0.0017 0.0313 0.0092 0.001 0.0009 0.002 0.0009 0.0005 0.0011 0.0022 0.0009 0.0009 0.0017 0.003 0.008
Peroxisomes 0.006 0.0002 0.0005 0.0142 0.0055 0.0059 0.0148 0.0001 0.0066 0.0025 0 0.0002 0.0064 0.0001 0.002 0.0056 0.0472 0.0034
Punctate Nuclear 0.0135 0.0005 0.0016 0.0032 0.0149 0.0087 0.0014 0.0003 0.0053 0.0003 0.0002 0.0002 0.0045 0.0003 0.0005 0.0013 0.0046 0.0033
Vacuole 0.0107 0.0046 0.0067 0.0077 0.0024 0.0047 0.0055 0.003 0.0038 0.006 0.0018 0.0038 0.0058 0.0043 0.0036 0.0301 0.0035 0.0028
Vacuole Periphery 0.0025 0.0007 0.0016 0.002 0.0018 0.001 0.0012 0.0003 0.0008 0.0016 0.0003 0.0011 0.0009 0.0004 0.0008 0.011 0.0007 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.7053 31.7163 29.8108 27.4125 32.2003 13.0019 31.2666 21.6439 25.0438 30.6831
Translational Efficiency 1.6573 1.6572 1.4946 1.3374 1.4257 3.1884 1.6128 1.5097 1.4993 1.5033

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description 2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively; yeast null mutant can be functionally complemented by human FTSJ1, mutations in which have been implicated in nonsyndromic X-linked intellectual disability (NSXLID)
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Trm7

Trm7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available