Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0.05 0.05 0 0.1 0.06 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.07 0 0.05 0.05 0 0.1 0.12 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.97 0.97 0.96 0.87 0.86 0.76 0.73 0.69 0.6 0.56 0.57 0.98 0.97 1.0 0.97 0.99 0.99 0.97 0.96 0.89 0.93 0.89 0.92
Endoplasmic Reticulum 0 0 0 0 0.08 0.09 0.13 0.2 0.15 0.17 0.12 0.16 0 0 0 0 0.06 0 0 0 0.06 0 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.05 0.17 0.11 0.24 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 5 14 11 7 25 7 19 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 2 1 0 0 0 0 1 1 3 0 0 0 0 0 0 0 0 1 2 2 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 6 4 14 9 13 10 7 24 15 12 2 1 2 7 8 1 0 0 0 0 1 0
Cytoplasm 313 440 210 174 178 210 204 162 108 145 69 92 149 164 181 396 283 337 292 434 198 102 202 197
Endoplasmic Reticulum 0 12 6 4 16 23 36 44 23 41 15 26 3 5 3 10 17 13 4 5 12 4 12 8
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 2 0 8 3 4 11 0 9 0 1 0 3 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 19 10 26 27 30 27 0 1 0 0 1 2 0 0 1 1 0 0
Nucleus 0 0 0 0 2 0 1 0 0 0 2 2 0 0 0 0 0 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 1 3 4 3 1 9 3 8 0 0 0 2 2 1 0 4 2 0 3 1
Unique Cell Count 313 452 217 181 205 244 268 222 157 241 124 162 152 169 181 407 287 339 301 451 223 110 227 214
Labelled Cell Count 313 456 223 184 213 250 300 246 178 286 142 195 154 172 186 418 311 354 301 451 223 110 227 214


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 66.3 88.4 62.3 52.8 38.1 28.6 23.2 22.5 18.4 19.4 17.2 17.7 69.3 64.9 63.8 112.6 113.7 116.5 75.2 74.5 76.9
Std Deviation (1e-4) 11.3 17.8 14.0 12.9 9.4 6.9 5.3 4.7 4.3 4.3 4.3 4.5 15.0 13.2 11.6 26.0 26.4 28.8 16.4 17.8 22.2
Intensity Change (Log2) – – – -0.24 -0.71 -1.12 -1.43 -1.47 -1.76 -1.68 -1.85 -1.81 0.15 0.06 0.04 0.85 0.87 0.9 0.27 0.26 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.4 3.3 0 4.9 0 5.2 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.0 0.6 1.2 1.0 0.9 3.1 3.4 2.1 0 0 0 0 0 0
Cytoplasm -0.3 -3.8 -4.0 -6.4 -6.9 -7.5 -9.4 -9.5 -9.6 0.7 0.2 2.4 0.4 1.4 2.4
Endoplasmic Reticulum 0 2.3 2.9 4.2 5.6 4.2 5.0 3.4 4.6 0 0 0 -0.2 1.7 0.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.0 0 6.2 5.1 7.6 6.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 68.5339 96.9746 84.2057 84.6772 64.6495 90.48 69.1499 94.619 82.3662 73.7829 76.914 81.3087 74.0434 96.4109 93.1654 91.0946 71.3247 73.0777
Actin 0.0069 0.0002 0.0021 0.0004 0.0019 0.001 0.0052 0.0002 0.0108 0.0013 0.0017 0.0018 0.0134 0.0008 0.0037 0.0204 0.007 0.015
Bud 0.0021 0.0006 0.0013 0.0003 0.001 0.0004 0.0013 0.0002 0.0007 0.0011 0.0005 0.0014 0.0026 0.002 0.0026 0.0013 0.0028 0.0013
Bud Neck 0.0007 0.0003 0.0009 0.0004 0.0007 0.0028 0.0007 0.0004 0.0012 0.0004 0.0005 0.0017 0.0029 0.0008 0.0005 0.0007 0.001 0.0019
Bud Periphery 0.0028 0.0007 0.0011 0.0003 0.0006 0.0005 0.0028 0.0002 0.0006 0.002 0.0023 0.0022 0.0041 0.0007 0.0012 0.0007 0.0013 0.0009
Bud Site 0.0016 0.0004 0.0027 0.0002 0.0009 0.0001 0.0006 0.0002 0.0006 0.0004 0.0004 0.0002 0.0036 0.0027 0.0009 0.0019 0.0016 0.0002
Cell Periphery 0.0109 0.0083 0.0156 0.0025 0.0031 0.0058 0.0062 0.0069 0.0033 0.0584 0.002 0.0038 0.0147 0.0091 0.0055 0.0349 0.0013 0.0013
Cytoplasm 0.7048 0.9349 0.8146 0.8062 0.7591 0.8389 0.7189 0.9562 0.8549 0.6016 0.8637 0.7875 0.6593 0.8954 0.8395 0.7108 0.7623 0.7492
Cytoplasmic Foci 0.0187 0.0007 0.0108 0.0026 0.0323 0.0031 0.0089 0.001 0.0047 0.0031 0.0105 0.0067 0.0225 0.0063 0.0117 0.0081 0.0434 0.0259
Eisosomes 0.0003 0 0.0002 0 0.0002 0.0003 0.0002 0 0.0002 0.0009 0.0001 0.0001 0.001 0.0001 0.0003 0 0.0001 0.0001
Endoplasmic Reticulum 0.0452 0.0135 0.0107 0.006 0.0202 0.0839 0.067 0.0146 0.0157 0.0263 0.0186 0.0441 0.0363 0.0073 0.0082 0.0164 0.0037 0.0258
Endosome 0.0233 0.0011 0.0074 0.0122 0.0336 0.0079 0.0207 0.0017 0.0102 0.04 0.0121 0.0331 0.0144 0.0025 0.0043 0.0095 0.0288 0.0202
Golgi 0.004 0.0002 0.0017 0.009 0.0154 0.0024 0.0031 0.0006 0.002 0.0021 0.0012 0.0101 0.0039 0.0009 0.0012 0.0022 0.005 0.0028
Lipid Particles 0.0075 0.0009 0.006 0.0029 0.0194 0.0017 0.0039 0.0007 0.0031 0.0057 0.0026 0.0008 0.0091 0.0023 0.0044 0.0017 0.0289 0.0012
Mitochondria 0.0074 0.001 0.0064 0.0053 0.0085 0.0015 0.0183 0.0004 0.0082 0.0293 0.0131 0.0241 0.0209 0.0069 0.0066 0.0016 0.0023 0.0032
None 0.0063 0.0004 0.003 0.0002 0.0098 0.0022 0.0029 0.0001 0.0028 0.0022 0.0142 0.0028 0.0227 0.0085 0.0244 0.0005 0.005 0.0164
Nuclear Periphery 0.0196 0.0032 0.0038 0.0044 0.0108 0.0121 0.0199 0.0017 0.0089 0.0135 0.0065 0.0102 0.0154 0.003 0.0051 0.006 0.0171 0.0722
Nucleolus 0.0065 0.0003 0.0027 0.0003 0.0052 0.0007 0.0045 0 0.0025 0.0026 0.0008 0.0013 0.0143 0.0016 0.0026 0.0005 0.0011 0.0011
Nucleus 0.0093 0.0025 0.0039 0.0026 0.006 0.0035 0.007 0.0009 0.0043 0.0057 0.0026 0.0043 0.0103 0.0033 0.0035 0.004 0.0037 0.0245
Peroxisomes 0.0019 0 0.0023 0.0001 0.0011 0 0.0014 0 0.0006 0.0002 0.0004 0.0002 0.0088 0.0003 0.0025 0.0023 0.0096 0.0003
Punctate Nuclear 0.0031 0 0.0006 0 0.0053 0.0001 0.0016 0 0.0021 0.0001 0.009 0.0001 0.003 0.0009 0.0011 0.0011 0.0095 0.0051
Vacuole 0.0984 0.0294 0.0901 0.124 0.0517 0.0241 0.077 0.0131 0.0529 0.1494 0.0272 0.0383 0.1012 0.0398 0.0574 0.1571 0.0541 0.0228
Vacuole Periphery 0.0187 0.0015 0.0122 0.0199 0.0131 0.0068 0.0279 0.0009 0.0097 0.0536 0.0098 0.0252 0.0156 0.0049 0.0128 0.0184 0.0106 0.0086

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2008.3897 2408.8344 2694.0296 2023.145 2619.0241 2346.8222 2002.9589 1863.4974 2241.965 1786.0153
Translational Efficiency 1.4418 1.262 1.1842 1.6672 1.3674 1.2329 1.2777 1.5031 1.1475 1.5046

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl19a

Rpl19a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl19a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available