Standard name
Human Ortholog
Description Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Snd1p, Env10p/Snd2p, and Sec61p-translocon subunits; can compensate for loss of SRP; role in phosphate transport, interacting with pho88, and in the maturation of secretory proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.07 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0.11 0.07 0.12 0 0.08 0 0.1 0.12 0 0.11 0.14 0.13 0.16 0.53 0.64 0.32 0 0 0 0 0 0
Cytoplasm 0.06 0.12 0.07 0.07 0.1 0.2 0.07 0.13 0.11 0.08 0.18 0.11 0 0 0.05 0.33 0.19 0.5 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.93 0.86 0.88 0.84 0.82 0.7 0.82 0.83 0.82 0.81 0.77 0.79 0.94 0.89 0.87 0 0 0.09 0.93 0.86 0.94 0.88 0.91 0.88
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.06 0.06 0 0 0.08 0 0 0 0 0 0 0 0 0.06 0.31 0.09 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 9 1 4 2 6 4 8 12 17 1 7 3 8 3 2 1 1 3 5 0 6 10 19
Bud 1 8 0 2 1 4 2 1 1 1 1 1 0 0 1 0 0 0 1 3 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 26 13 12 19 2 21 14 22 27 2 15 53 101 109 27 38 7 1 2 1 5 14 25
Cytoplasm 14 50 8 12 16 33 18 51 23 18 12 14 13 29 32 17 11 11 4 27 2 9 4 9
Endoplasmic Reticulum 223 358 106 146 130 117 212 331 174 187 51 105 356 706 594 0 0 2 224 389 115 328 517 554
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Golgi 7 2 7 10 6 6 20 9 3 7 2 5 9 11 13 3 18 2 1 3 0 8 6 4
Mitochondria 0 1 0 0 0 1 0 0 0 0 1 1 0 0 1 2 0 3 2 2 0 3 5 6
Nucleus 0 0 0 0 0 1 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 2 3 4 2 2 0 0 0 0 0 0 1 1 0 0 1 1
Nucleolus 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 2 2 0 1 7 9 7 4 5 0 2 0 2 2 2 0 0 0 13 0 3 1 1
Unique Cell Count 240 416 120 173 159 167 258 399 212 231 66 133 378 791 684 51 59 22 242 454 122 371 569 631
Labelled Cell Count 257 456 137 186 175 179 287 425 243 266 73 152 434 857 755 53 68 26 242 454 122 371 569 631


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 67.9 58.8 59.3 60.4 59.1 36.8 45.7 42.5 38.7 39.5 36.3 36.9 73.9 69.7 63.9 72.7 76.3 68.1 58.8 60.0 58.9
Std Deviation (1e-4) 14.9 12.2 11.3 12.9 15.3 14.7 10.6 10.1 9.5 9.0 8.4 9.2 13.9 12.8 12.6 20.0 16.0 19.6 13.4 12.8 13.0
Intensity Change (Log2) – – – 0.03 -0.01 -0.69 -0.38 -0.48 -0.62 -0.59 -0.71 -0.69 0.32 0.23 0.11 0.29 0.36 0.2 -0.01 0.02 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 2.6 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.2 0.3 -3.6 -0.9 -3.2 -0.1 0.2 -1.9 0.1 0.9 0.6 1.4 6.0 7.5 0
Cytoplasm 0.1 1.0 3.1 0.1 1.9 1.3 0.4 2.4 1.1 -1.5 0 -0.9 4.5 2.4 0
Endoplasmic Reticulum -1.0 -1.5 -3.7 -1.5 -1.4 -1.5 -1.8 -2.0 -2.0 2.2 0.3 -0.4 -10.9 -11.3 -8.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 -0.8 -0.9 0.7 0 0 0 0 -0.8 0 0 0 0 4.5 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 76.1787 72.683 72.951 71.2406 71.2472 70.5694 100.4203 93.016 91.1449 93.3239 96.9741 91.6893 89.0733 85.1175 82.126 81.7429 74.4334 81.8188
Actin 0.0017 0.0001 0.0001 0 0 0.0001 0.0019 0.0015 0.0002 0 0 0.0001 0.0049 0.0006 0.0004 0.0012 0.0011 0.0011
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0.0001 0 0 0.0007 0
Bud Neck 0.0002 0.0002 0.0008 0.0001 0.0001 0.0005 0.0001 0.0003 0.0003 0.0001 0.0002 0.0009 0.0007 0.0006 0.0006 0.0021 0.0026 0.0035
Bud Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0 0.0001 0.0003 0.0003 0.0001 0.0002 0.0018 0.0002
Bud Site 0.0002 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0.0002 0.0005 0 0 0.0018 0
Cell Periphery 0.0321 0.0598 0.0443 0.046 0.057 0.0223 0.0378 0.076 0.0435 0.051 0.065 0.0258 0.05 0.083 0.0395 0.0253 0.0114 0.0146
Cytoplasm 0.0064 0.019 0.0052 0.0015 0.0013 0.0017 0.0087 0.0123 0.0125 0.0056 0.0037 0.004 0.0198 0.029 0.0209 0.0063 0.004 0.0118
Cytoplasmic Foci 0.0035 0.0001 0.0001 0 0 0 0.0003 0.0001 0.0001 0 0 0.0001 0.0017 0.0003 0.0001 0.0001 0.0015 0.0001
Eisosomes 0.0019 0.0017 0.001 0 0.0005 0.0001 0.0003 0.0019 0.0013 0.0003 0.0005 0.0003 0.0054 0.0084 0.0015 0.0007 0.0004 0.0008
Endoplasmic Reticulum 0.7912 0.8567 0.896 0.9185 0.892 0.9038 0.8715 0.8538 0.9003 0.896 0.8604 0.9312 0.7413 0.8017 0.8224 0.8767 0.865 0.8493
Endosome 0.0031 0.0002 0.0002 0 0.0002 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0017 0.0004 0.0005 0.0004 0.0017 0.0006
Golgi 0.0024 0.0009 0.0006 0.0001 0.003 0.0008 0.0003 0.0015 0.0018 0.0004 0.0002 0.0003 0.0018 0.0019 0.0045 0.0032 0.0015 0.0018
Lipid Particles 0.0071 0.0088 0.0074 0.0021 0.0034 0.0059 0.0042 0.0174 0.0061 0.0051 0.006 0.004 0.0049 0.0206 0.0074 0.0015 0.0027 0.0021
Mitochondria 0.0009 0 0.0003 0 0 0 0.0002 0 0 0 0 0 0.0006 0.0004 0.0001 0.0001 0.0024 0.0001
None 0.001 0.0001 0.0001 0 0.0001 0 0.0003 0.0001 0.0001 0 0.0001 0 0.0029 0.0002 0.0002 0.0001 0.0014 0.0001
Nuclear Periphery 0.1284 0.0397 0.0315 0.0201 0.0298 0.0546 0.0621 0.0229 0.0251 0.0322 0.0531 0.0277 0.1436 0.0377 0.0865 0.0734 0.0472 0.1043
Nucleolus 0.0007 0 0.0001 0 0 0 0 0 0 0 0 0 0.0016 0.0001 0.0001 0.0001 0.0235 0.0001
Nucleus 0.0029 0.0004 0.0055 0.0001 0.0002 0.0008 0.0006 0.0004 0.0002 0.0001 0.0002 0.0006 0.0031 0.001 0.0018 0.0024 0.0091 0.0029
Peroxisomes 0.0024 0 0 0 0 0 0.0014 0 0 0 0 0 0.0002 0 0 0 0.0004 0
Punctate Nuclear 0.0003 0 0 0 0 0 0.0001 0 0 0 0 0 0.001 0.0001 0.0001 0.0001 0.012 0.0002
Vacuole 0.0114 0.0115 0.0063 0.0113 0.0118 0.0068 0.0092 0.0111 0.0078 0.0085 0.0101 0.0044 0.0108 0.0124 0.0117 0.005 0.0051 0.0041
Vacuole Periphery 0.0021 0.0006 0.0004 0.0001 0.0006 0.002 0.0006 0.0002 0.0004 0.0004 0.0004 0.0003 0.0036 0.0008 0.0016 0.0011 0.0028 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 955.5054 828.0689 739.679 649.9472 648.4432 797.4345 740.5174 689.7468 706.1414 701.7771
Translational Efficiency 1.1768 1.1462 1.2219 1.3983 1.4674 1.3835 1.2841 1.3037 1.2365 1.2878

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Snd1p, Env10p/Snd2p, and Sec61p-translocon subunits; can compensate for loss of SRP; role in phosphate transport, interacting with pho88, and in the maturation of secretory proteins
Localization
Cell Percentages ER (97%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Snd3

Snd3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snd3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available