Standard name
Human Ortholog
Description Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 – 0.12 0.17 0.11 0.13 0.15 0.13 0.24 0.26 0.29 0.3 0.07 0.08 0.06 0 0 0 0.42 0.3 0.46 0.35 0.29 0.22
Bud 0.19 – 0.09 0.07 0.11 0.09 0.12 0.11 0.14 0.14 0.17 0.13 0.07 0.05 0.12 0 0 0 0.22 0.28 0.14 0.19 0.32 0.38
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.09 0.13 0.09
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.07 – 0.06 0.11 0.07 0 0 0 0.05 0 0.06 0.05 0.13 0.22 0.23 0.07 0.06 0.11 0 0 0 0 0 0
Cytoplasm 0.29 – 0.28 0.24 0.29 0.29 0.21 0.23 0.23 0.18 0.21 0.13 0.41 0.38 0.42 0.44 0.5 0.45 0.1 0.1 0.05 0 0.08 0.13
Endoplasmic Reticulum 0.23 – 0.22 0.23 0.18 0.27 0.21 0.19 0.18 0.16 0.16 0.16 0.29 0.23 0.22 0 0.05 0.07 0.06 0.09 0.09 0.05 0.06 0.05
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0
Golgi 0 – 0 0 0.09 0 0.06 0.11 0.11 0.15 0.12 0.15 0 0 0 0.32 0.34 0.32 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.25 – 0.21 0.24 0.2 0.25 0.29 0.21 0.22 0.19 0.2 0.22 0.14 0.12 0.07 0.21 0.12 0.08 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Vac/Vac Membrane 0 – 0 0.05 0.1 0.07 0.07 0.13 0.09 0.09 0 0.06 0 0 0 0 0.05 0 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 – 8 32 27 28 53 56 47 56 73 95 7 10 9 1 0 3 82 7 53 19 55 51
Bud 21 – 6 13 26 21 45 48 27 30 43 42 7 6 18 1 1 0 42 7 16 10 60 89
Bud Neck 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 13 0 4 4 24 19
Bud Site 1 – 1 1 1 2 3 0 0 2 3 1 1 0 1 0 0 0 – – – – – –
Cell Periphery 8 – 4 20 16 6 8 13 9 9 14 15 13 29 35 8 10 10 4 1 1 0 0 2
Cytoplasm 33 – 19 45 69 65 77 96 46 39 53 40 42 50 63 48 82 40 19 2 5 1 15 31
Endoplasmic Reticulum 26 – 15 43 42 60 76 79 36 34 40 51 30 30 33 4 9 6 10 2 9 3 11 11
Endosome 0 – 0 1 0 2 2 1 2 2 3 2 0 1 0 0 0 0 3 0 5 5 5 2
Golgi 2 – 2 5 20 5 21 48 21 32 29 47 1 4 4 35 56 28 3 0 2 0 1 1
Mitochondria 1 – 1 1 2 1 3 2 5 9 17 23 0 1 0 2 4 0 0 0 0 0 1 0
Nucleus 0 – 1 0 1 1 1 3 1 0 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Nuclear Periphery 0 – 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 28 – 14 44 46 55 103 91 44 41 50 69 14 15 10 23 19 7 0 0 0 0 2 2
Peroxisomes 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 2 1
SpindlePole 0 – 0 0 4 1 7 6 0 1 1 2 0 0 0 1 2 0 5 1 6 1 4 4
Vac/Vac Membrane 2 – 1 9 24 16 26 54 18 19 7 18 1 1 4 3 8 1 2 0 0 3 0 2
Unique Cell Count 113 67 185 235 222 361 424 198 217 248 318 103 130 151 108 165 88 194 26 116 55 189 233
Labelled Cell Count 131 72 214 278 263 426 497 257 274 335 408 116 147 177 126 191 95 194 26 116 55 189 233


Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 47.0 – 35.5 36.6 34.1 33.4 32.1 33.2 30.4 30.9 30.5 30.3 45.7 50.3 49.3 70.4 68.7 62.8 – 44.5 42.7
Std Deviation (1e-4) 13.1 – 14.3 12.0 13.3 11.6 10.9 10.7 10.4 11.6 10.9 10.2 14.2 17.7 17.9 23.4 27.8 26.7 – 13.3 14.2
Intensity Change (Log2) – – – 0.04 -0.06 -0.09 -0.15 -0.1 -0.22 -0.2 -0.22 -0.23 0.36 0.5 0.47 0.99 0.95 0.82 – 0.32 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.0 -0.1 0.1 0.6 0.3 2.1 2.4 2.9 3.0 -1.2 -1.0 -1.5 0 0 0
Bud -0.5 0.5 0.1 0.8 0.6 1.0 1.0 1.7 1.0 -0.5 0 0.6 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.2 0 0 0 0 0 0 0 0 1.4 2.9 3.1 0 0 1.2
Cytoplasm -0.6 0.2 0.1 -1.3 -1.0 -0.8 -1.8 -1.2 -3.3 1.6 1.4 1.9 2.1 3.0 2.2
Endoplasmic Reticulum 0.1 -0.8 0.8 -0.2 -0.7 -0.8 -1.3 -1.2 -1.3 1.0 0.1 -0.1 -3.9 -3.8 -2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 2.1 1.9 2.6 2.1 2.6 0 0 0 4.6 4.9 4.5
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.5 -0.2 0.7 1.3 0.1 0.2 -0.4 -0.1 0.1 -1.3 -1.8 -3.1 0.1 -1.9 -2.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.3 0 0 2.7 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 39.5604 39.4399 34.3634 43.7407 38.4007 35.724 41.1608 40.9626 36.643 40.522 25.1306 41.065 47.2621 49.4075 50.5824 33.9343 41.3764 44.3752
Actin 0.4243 0.2024 0.4626 0.6748 0.5785 0.3796 0.6511 0.3547 0.5615 0.6825 0.512 0.5212 0.4375 0.3388 0.6446 0.6201 0.5384 0.504
Bud 0.0157 0.0211 0.0452 0.005 0.0422 0.0018 0.0053 0.0246 0.039 0.036 0.0126 0.0034 0.0319 0.0105 0.0561 0.0144 0.0285 0.013
Bud Neck 0.019 0.013 0.005 0.0109 0.0059 0.1472 0.011 0.0093 0.0256 0.019 0.0093 0.3165 0.0449 0.0167 0.0144 0.0068 0.0771 0.2698
Bud Periphery 0.0234 0.0188 0.0496 0.0113 0.0765 0.0036 0.0043 0.0167 0.0461 0.0856 0.015 0.0058 0.0432 0.0055 0.0502 0.0215 0.0431 0.0195
Bud Site 0.0822 0.3784 0.13 0.0042 0.0138 0.0042 0.0804 0.3746 0.1711 0.0099 0.0075 0.0122 0.1422 0.4645 0.1146 0.0111 0.0078 0.0218
Cell Periphery 0.0117 0.0187 0.0061 0.0043 0.0291 0.0033 0.0057 0.0189 0.0092 0.007 0.0042 0.0045 0.0352 0.0073 0.0096 0.0346 0.0225 0.0108
Cytoplasm 0.2504 0.2142 0.2045 0.25 0.1801 0.4412 0.0567 0.157 0.0572 0.0159 0.071 0.0786 0.0794 0.0649 0.0298 0.0567 0.1291 0.0225
Cytoplasmic Foci 0.0223 0.0288 0.0183 0.0024 0.0028 0.0008 0.0374 0.0102 0.0176 0.0933 0.0786 0.0015 0.0297 0.0598 0.0268 0.0806 0.0162 0.0114
Eisosomes 0.0041 0.003 0.0014 0.0252 0.0018 0.0114 0.0238 0.0062 0.0031 0.0011 0.0189 0.0072 0.0263 0.0044 0.0081 0.0054 0.003 0.0248
Endoplasmic Reticulum 0.0031 0.0009 0.0023 0.0005 0.0026 0.0002 0.0044 0.0013 0.0012 0.0007 0.0028 0.001 0.0039 0.0008 0.0004 0.0028 0.003 0.0028
Endosome 0.0295 0.0018 0.0095 0.0001 0.0016 0.0001 0.0242 0.001 0.0099 0.0023 0.0461 0.0052 0.0119 0.0026 0.0011 0.0142 0.0087 0.0214
Golgi 0.0063 0.003 0.0199 0.0018 0.003 0.0008 0.0233 0.0059 0.0098 0.0047 0.0183 0.0081 0.0113 0.0039 0.0036 0.0129 0.014 0.0109
Lipid Particles 0.0203 0.0071 0.0052 0.0029 0.0075 0.0002 0.0134 0.009 0.0053 0.0045 0.008 0.0009 0.0186 0.0092 0.0114 0.0444 0.0275 0.0042
Mitochondria 0.0074 0.0144 0.0078 0.0019 0.0022 0.0014 0.0159 0.0021 0.0179 0.0019 0.0935 0.0291 0.011 0.0006 0.0138 0.0052 0.0113 0.0093
None 0.0056 0.0088 0.0052 0.0008 0.0014 0.0002 0.0025 0.0003 0.0094 0.0013 0.0212 0.0002 0.0089 0.0006 0.0007 0.0281 0.0018 0.0014
Nuclear Periphery 0.018 0.0012 0.0042 0.0005 0.0238 0.0005 0.0109 0.001 0.0009 0.0016 0.0186 0.0009 0.0122 0.0003 0.0005 0.0028 0.0043 0.0038
Nucleolus 0.0051 0.027 0.0003 0.0004 0.0012 0.0002 0.0012 0.0007 0.0032 0.0006 0.0077 0.0001 0.0183 0.0006 0.001 0.0053 0.001 0.0266
Nucleus 0.0263 0.0107 0.0041 0.0011 0.0169 0.0024 0.0079 0.0011 0.0014 0.0017 0.016 0.0004 0.0134 0.0007 0.0012 0.0034 0.0013 0.0099
Peroxisomes 0.0037 0.0041 0.0142 0.001 0.001 0.0005 0.0105 0.0023 0.0048 0.0077 0.004 0.0004 0.0089 0.0027 0.0045 0.003 0.0548 0.0022
Punctate Nuclear 0.0123 0.0021 0.002 0.0005 0.0023 0.0002 0.0043 0.0007 0.0012 0.0213 0.0031 0.0002 0.0028 0.0014 0.0048 0.0063 0.0044 0.0015
Vacuole 0.0075 0.0178 0.0021 0.0004 0.0045 0.0001 0.0037 0.0022 0.0035 0.0011 0.0168 0.0009 0.0063 0.0038 0.0019 0.0172 0.0016 0.0044
Vacuole Periphery 0.0019 0.0027 0.0005 0.0002 0.0013 0.0001 0.0021 0.0002 0.0009 0.0004 0.015 0.0015 0.002 0.0002 0.0008 0.0031 0.0009 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 255.9699 244.3725 243.9779 242.8043 238.4353 265.7633 255.5081 271.373 283.309 260.1496
Translational Efficiency 2.8008 2.7381 2.4336 2.9272 2.7393 3.1313 2.6112 2.6098 2.4473 2.6312

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability
Localization
Cell Percentages cytoplasm (63%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-8

Cmd1

Cmd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cmd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available