Standard name
Human Ortholog
Description Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad7p) during NER; required for NER of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0.38 0.61 0.55 0.64 0.69 0.65 0.66 0.46 – 0.38 0.34 0.2 0.41 0.32 0.18 0.25 0.2 0.15 0.28 0.2 0.11 0.1 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.07 0.06 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.07 0.13 0.22 0.17 0.05 0 0 0 0.07 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.54 0.41 0.22 0.28 0.24 0.28 0.37 0.25 0.56 – 0.6 0.56 0.49 0.06 0.15 0.06 0 0.09 0.14 0.1 0.12 0.05 0.13 0.1
Nucleus 0.12 0.13 0 0.14 0.07 0.06 0 0.08 0.09 – 0.19 0.18 0.33 0.39 0.33 0.44 0.32 0.35 0.15 0.21 0.14 0.17 0.12 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.11 0.06 0.06 0 0 0 0 – 0 0.06 0 0 0 0.06 0.06 0.06 0.12 0.07 0.11 0.06 0.15 0.14
Vac/Vac Membrane 0.21 0.19 0 0 0.05 0 0 0 0 – 0 0 0.15 0.14 0.19 0.21 0.24 0.28 0.22 0.22 0.26 0.44 0.27 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 0 0 0 0 1
Bud 0 1 0 2 0 0 7 5 9 – 10 7 0 0 0 2 0 0 2 0 1 3 2 7
Bud Neck 0 1 8 1 5 3 10 11 3 – 3 6 0 0 0 1 1 0 0 0 1 2 3 8
Bud Site 0 0 1 0 0 0 0 0 1 – 3 9 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 2 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 16 57 73 79 104 145 198 252 104 – 81 81 23 52 46 49 56 44 19 52 34 13 17 32
Endoplasmic Reticulum 4 4 0 1 0 1 0 0 0 – 0 0 2 0 1 18 16 14 2 1 3 0 2 3
Endosome 7 2 5 6 4 3 5 1 0 – 0 0 2 8 10 37 49 37 6 7 5 2 12 8
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 3 2 1 1 1 0 0 1 0
Mitochondria 52 62 26 40 39 59 112 95 126 – 129 134 55 8 21 16 8 20 17 19 20 5 22 21
Nucleus 12 19 4 20 12 13 10 31 21 – 40 43 37 49 47 120 73 76 18 38 24 21 21 25
Nuclear Periphery 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 1 0 0 1 0 1 0 0 0
Nucleolus 0 0 0 0 0 1 0 1 1 – 2 0 1 0 1 0 0 0 1 4 3 1 3 7
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 3 2 13 8 9 5 7 16 9 – 7 14 0 0 3 16 14 12 15 12 19 7 25 32
Vac/Vac Membrane 20 29 2 6 8 8 11 8 6 – 3 7 17 18 27 57 54 61 27 41 45 55 46 66
Unique Cell Count 96 151 119 144 162 209 306 379 225 214 239 113 127 143 275 225 217 127 189 176 126 173 228
Labelled Cell Count 114 177 132 163 181 238 360 423 280 278 301 138 135 157 320 274 265 127 189 176 126 173 228


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.7 4.8 4.4 4.8 4.0 3.8 4.3 3.8 – 3.6 3.8 3.9 4.3 4.4 6.0 6.1 6.1 4.0 4.0 4.3
Std Deviation (1e-4) 1.1 0.9 2.0 1.7 1.6 1.9 1.4 1.6 2.0 – 1.8 1.9 0.9 1.2 1.3 1.3 1.3 1.2 1.2 1.7 1.5
Intensity Change (Log2) – – – -0.12 -0.01 -0.27 -0.35 -0.15 -0.35 – -0.41 -0.34 -0.28 -0.16 -0.14 0.33 0.35 0.33 -0.25 -0.25 -0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.9 5.3 5.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3318 1.3685 1.3171 1.1856 1.0934 1.4113 -0.8777 -1.0566 -0.8259 -0.8288 -0.6614 -1.1203 -1.0825 -0.7669 -1.085 -1.0146 -0.6808 -0.8392
Actin 0.0158 0.0008 0.0039 0.0049 0.0193 0.001 0.0151 0.0002 0.0129 0.0239 0.004 0.0023 0.025 0.0004 0.0044 0.0092 0.0323 0.0123
Bud 0.0015 0.0002 0.0046 0.0002 0.0003 0.0001 0.0006 0.0001 0.0009 0.0023 0.001 0.0006 0.0004 0.0033 0.0005 0.0001 0.0028 0.0002
Bud Neck 0.0059 0.0016 0.0017 0.0606 0.0015 0.0174 0.003 0.0006 0.0015 0.0053 0.006 0.0068 0.0033 0.0008 0.0008 0.0009 0.0014 0.005
Bud Periphery 0.0021 0.0003 0.0017 0.0004 0.0004 0.0003 0.0011 0 0.0017 0.0066 0.0015 0.0013 0.0006 0.0015 0.001 0.0001 0.0014 0.0003
Bud Site 0.0057 0.003 0.0019 0.0006 0.0023 0.0007 0.0072 0.0008 0.0029 0.0038 0.0061 0.0008 0.0073 0.0024 0.0011 0.0003 0.0008 0.0003
Cell Periphery 0.0025 0.0004 0.0003 0.0006 0.0001 0.0002 0.0003 0 0.0002 0.0004 0.0002 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.0763 0.0912 0.0878 0.0552 0.0931 0.1018 0.0761 0.0954 0.0687 0.052 0.0942 0.0629 0.1082 0.1369 0.0988 0.0738 0.1219 0.0922
Cytoplasmic Foci 0.0228 0.0051 0.0194 0.0028 0.0157 0.0086 0.0156 0.0081 0.0205 0.0119 0.0373 0.0052 0.0162 0.0144 0.0062 0.006 0.0138 0.0068
Eisosomes 0.0006 0.0003 0.0003 0.0003 0.0004 0.0002 0.0003 0 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0065 0.0052 0.0038 0.0016 0.0049 0.0019 0.0055 0.0015 0.0031 0.0015 0.0037 0.0017 0.0043 0.0052 0.0028 0.0028 0.0033 0.0026
Endosome 0.0095 0.0035 0.0057 0.0015 0.0164 0.0038 0.0298 0.0026 0.0175 0.0199 0.0464 0.0074 0.017 0.0062 0.0088 0.0076 0.016 0.0092
Golgi 0.0053 0.0002 0.0015 0.0003 0.0089 0.0008 0.006 0.0001 0.004 0.0116 0.0305 0.0025 0.0059 0.0002 0.0019 0.0034 0.008 0.0022
Lipid Particles 0.0177 0.0012 0.0091 0.0102 0.0119 0.0063 0.0074 0.0003 0.0094 0.0151 0.0169 0.0034 0.0211 0.0014 0.0037 0.0107 0.0182 0.0029
Mitochondria 0.0045 0.001 0.0019 0.0013 0.0245 0.0034 0.0063 0.0003 0.0074 0.035 0.019 0.0078 0.0054 0.0016 0.0037 0.0015 0.0073 0.0019
None 0.3467 0.4295 0.3871 0.3527 0.3517 0.2886 0.1317 0.1774 0.1407 0.1416 0.0692 0.1176 0.1705 0.228 0.1712 0.2074 0.3387 0.1293
Nuclear Periphery 0.0132 0.0237 0.0078 0.0074 0.0141 0.0073 0.0351 0.013 0.0248 0.0181 0.0462 0.0178 0.0188 0.0223 0.0141 0.0217 0.0157 0.023
Nucleolus 0.0139 0.0121 0.0153 0.0464 0.0072 0.0233 0.0102 0.008 0.011 0.0148 0.0142 0.0173 0.0086 0.0082 0.0053 0.0055 0.0054 0.0092
Nucleus 0.3757 0.3744 0.378 0.411 0.3515 0.4482 0.5609 0.6101 0.5443 0.5215 0.4737 0.6281 0.4864 0.5112 0.6253 0.5849 0.3363 0.638
Peroxisomes 0.0173 0.0003 0.0161 0.0009 0.0094 0.0086 0.0061 0.0001 0.0111 0.0154 0.0107 0.0009 0.0165 0.0004 0.0024 0.0042 0.0035 0.0007
Punctate Nuclear 0.0531 0.0429 0.0497 0.0359 0.0644 0.0756 0.0769 0.0803 0.1121 0.0879 0.1107 0.1119 0.0804 0.0514 0.0441 0.0578 0.07 0.0616
Vacuole 0.0025 0.0025 0.0022 0.0041 0.0013 0.0017 0.0036 0.0009 0.0039 0.0074 0.0057 0.0029 0.0026 0.0033 0.0027 0.0013 0.002 0.0013
Vacuole Periphery 0.0007 0.0005 0.0003 0.0011 0.0006 0.0003 0.0014 0.0002 0.0015 0.0039 0.0027 0.0006 0.0009 0.0007 0.0011 0.0005 0.0011 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.4173 4.988 2.4675 7.1435 5.7273 5.112 5.2535 6.9497 7.1073 6.2049
Translational Efficiency 0.5861 0.6117 1.0495 0.5027 0.6388 0.5183 0.4762 0.302 0.4594 0.4669

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad7p) during NER; required for NER of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
Localization
Cell Percentages nucleus (56%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rad16

Rad16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available