Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 – 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0.14 0.3 0.2 0.19 0.16 0.15 0.18 0.1 – 0.07 0.1 0.22 0.37 0.35 0.3 0.25 0.12 0.06 0.08 0.11 0.15 0.12 0.15
Endoplasmic Reticulum 0.08 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0 0 0 0 – 0 0 0 0 0 0.09 0.15 0.1 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.27 0.38 0.59 0.37 0.7 0.6 0.68 0.84 – 0.9 0.84 0.19 0.08 0.29 0.05 0.1 0.12 0.06 0 0.13 0 0.05 0.07
Nucleus 0.58 0.37 0.28 0.32 0.35 0.34 0.39 0.31 0.32 – 0.27 0.27 0.53 0.44 0.25 0.47 0.4 0.62 0.44 0.45 0.26 0.29 0.27 0.23
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.08 0 0.08 0.1 0.1 0.09
Vac/Vac Membrane 0.21 0.37 0.07 0 0.13 0.05 0.06 0 0 – 0 0 0.13 0.13 0.22 0.14 0.15 0.12 0.23 0.27 0.28 0.29 0.28 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 1 2
Bud 0 1 0 0 1 2 4 15 9 – 6 11 0 0 4 0 0 0 0 3 1 3 12 5
Bud Neck 0 0 2 0 0 0 0 3 1 – 0 0 0 0 0 0 0 0 0 4 0 2 4 6
Bud Site 0 0 0 4 2 7 7 10 22 – 7 22 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 11 38 60 36 54 42 56 90 39 – 21 37 43 99 122 13 5 5 7 27 36 32 43 49
Endoplasmic Reticulum 5 2 2 0 0 0 0 0 1 – 1 1 0 2 1 4 1 2 0 0 5 0 2 2
Endosome 1 1 6 1 26 2 6 5 1 – 0 0 3 4 2 4 3 4 6 9 9 4 13 12
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 1 1
Mitochondria 2 75 76 107 104 181 231 334 325 – 283 307 36 22 100 2 2 5 7 14 44 8 16 22
Nucleus 38 103 56 59 99 89 150 153 124 – 83 98 103 117 87 20 8 25 56 160 86 63 93 77
Nuclear Periphery 0 0 0 0 0 2 3 0 1 – 1 1 0 0 0 0 0 0 1 4 3 1 1 1
Nucleolus 0 0 0 0 5 3 2 6 7 – 3 7 2 0 0 0 2 0 3 11 6 3 9 9
Peroxisomes 0 0 1 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 3 7 3 11 6 16 17 13 – 6 14 0 8 4 0 0 2 10 15 25 21 33 31
Vac/Vac Membrane 14 102 14 8 36 13 25 18 14 – 9 15 26 36 77 6 3 5 30 95 93 63 98 99
Unique Cell Count 66 279 202 182 284 259 385 488 389 313 364 193 268 350 43 20 40 130 357 337 215 346 337
Labelled Cell Count 72 325 224 218 338 347 500 652 557 420 513 213 288 397 50 24 48 130 357 337 215 346 337


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 3.9 3.7 3.7 4.0 3.6 3.5 3.4 2.9 – 2.8 2.8 4.0 4.2 3.7 5.4 5.9 6.3 4.3 4.4 4.8
Std Deviation (1e-4) 0.6 0.7 1.0 1.5 1.7 1.5 1.3 1.5 1.3 – 0.8 1.0 0.8 1.0 0.9 1.6 1.1 2.2 1.2 1.4 1.1
Intensity Change (Log2) – – – -0.01 0.11 -0.04 -0.08 -0.11 -0.34 – -0.38 -0.39 0.13 0.18 0.0 0.54 0.67 0.77 0.21 0.26 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8796 0.9421 0.8117 0.3104 0.4012 0.8091 1.5624 1.785 1.4801 0.8225 0.8639 1.8124 2.5821 2.7747 2.5983 2.8935 2.6764 2.7897
Actin 0.0246 0.0005 0.0377 0.006 0.0065 0.0062 0.0458 0.0002 0.0136 0.001 0.0001 0.0043 0.0286 0.0002 0.0103 0.0002 0.0144 0.0124
Bud 0.0004 0.0001 0.0003 0.0002 0.0001 0.0001 0.0009 0.0001 0.0014 0.0001 0 0.0002 0.0003 0.0001 0.0003 0.0001 0.0005 0.0001
Bud Neck 0.0112 0.0014 0.0036 0.0035 0.0021 0.005 0.0108 0.001 0.0011 0.0008 0.0006 0.0047 0.0007 0.0006 0.0028 0.0029 0.0017 0.0053
Bud Periphery 0.0009 0.0001 0.0008 0.0003 0.0002 0.0001 0.001 0.0001 0.0018 0.0001 0 0.0006 0.0004 0.0001 0.0006 0.0001 0.0006 0.0002
Bud Site 0.0052 0.0014 0.0032 0.001 0.0003 0.0002 0.0083 0.0008 0.0051 0.0002 0 0.0003 0.0005 0.0009 0.0042 0.0004 0.0052 0.0005
Cell Periphery 0.0004 0.0001 0.0006 0.0001 0 0 0.0002 0 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.0319 0.0762 0.0344 0.0234 0.0471 0.0289 0.0456 0.0454 0.0401 0.0306 0.0151 0.0145 0.029 0.0659 0.0263 0.0595 0.0973 0.0375
Cytoplasmic Foci 0.0082 0.0046 0.0034 0.0034 0.0109 0.0019 0.0331 0.0032 0.0029 0.0005 0.0037 0.0033 0.0054 0.0015 0.0055 0.0024 0.0075 0.0011
Eisosomes 0.0006 0.0001 0.0007 0.0001 0.0002 0 0.0002 0 0.0001 0 0 0.0001 0.0003 0 0.0002 0 0.0002 0.0001
Endoplasmic Reticulum 0.0066 0.0052 0.0035 0.0019 0.0018 0.0018 0.0033 0.0049 0.002 0.0017 0.0002 0.0014 0.0047 0.0008 0.002 0.0007 0.0048 0.0011
Endosome 0.0159 0.0044 0.0073 0.0082 0.0074 0.0038 0.0301 0.0032 0.0072 0.0015 0.0003 0.013 0.0142 0.0007 0.0056 0.0013 0.0197 0.0024
Golgi 0.0036 0.0007 0.0043 0.0012 0.0033 0.0008 0.0077 0.0003 0.0018 0 0 0.0066 0.005 0 0.0012 0.0001 0.0026 0.0014
Lipid Particles 0.0136 0.0028 0.0107 0.0053 0.0213 0.0021 0.0174 0.0034 0.0007 0 0.0001 0.0131 0.0036 0.0001 0.0038 0.0004 0.0034 0.0015
Mitochondria 0.003 0.0018 0.014 0.0023 0.0023 0.0004 0.0021 0.0006 0.0017 0.001 0.0002 0.016 0.0023 0.0004 0.0013 0.0003 0.0022 0.0012
None 0.0343 0.105 0.0366 0.008 0.1012 0.0169 0.0733 0.0563 0.078 0.0113 0.049 0.0075 0.0868 0.082 0.1077 0.1333 0.1158 0.0785
Nuclear Periphery 0.0379 0.0288 0.0389 0.0354 0.0169 0.0122 0.0127 0.0294 0.0187 0.0338 0.0096 0.0169 0.0296 0.0249 0.0346 0.0144 0.0395 0.0071
Nucleolus 0.0161 0.0142 0.0243 0.021 0.0226 0.0283 0.021 0.014 0.0135 0.0087 0.0081 0.0221 0.0133 0.0152 0.0176 0.032 0.0144 0.0324
Nucleus 0.7278 0.6658 0.714 0.7904 0.6403 0.8325 0.5862 0.7855 0.7645 0.8693 0.8172 0.7914 0.7136 0.7345 0.6981 0.6637 0.6285 0.7669
Peroxisomes 0.0087 0.0068 0.0047 0.011 0.0308 0.0006 0.0163 0.003 0.0006 0.0001 0 0.0013 0.0023 0.0001 0.0059 0.0001 0.0014 0.0007
Punctate Nuclear 0.0404 0.0763 0.0527 0.075 0.0829 0.0567 0.0798 0.0462 0.0429 0.0382 0.0954 0.0804 0.0579 0.071 0.0634 0.0867 0.0342 0.0483
Vacuole 0.0061 0.0032 0.0032 0.0018 0.0012 0.0012 0.0034 0.002 0.0017 0.0005 0.0001 0.0014 0.0009 0.0008 0.0073 0.0011 0.0046 0.0009
Vacuole Periphery 0.0024 0.0005 0.001 0.0006 0.0005 0.0002 0.0007 0.0005 0.0005 0.0004 0.0001 0.001 0.0006 0.0003 0.0012 0.0002 0.0015 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.8858 14.7131 6.4615 12.282 13.7182 12.0854 16.6221 22.3351 22.9966 12.3932
Translational Efficiency 0.401 0.2572 0.5598 0.4263 0.2153 0.4017 0.3639 0.2395 0.2289 0.3137

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (37%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Tbs1

Tbs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tbs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available