Standard name
Human Ortholog
Description mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.05 0.05 0.05 0 0.06 0 0 0 0 0.11 0.13 0.16 0.15 0.15 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0 0 0.06 0 0 0 0.25 0.14 0.22 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.97 0.9 0.94 0.93 0.91 0.91 0.9 0.89 0.84 0.87 0.87 0.81 0.88 0.84 0.8 0.82 0.79 0.85 0.95 0.9 0.91 0.79 0.68 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.05 0 0.11 0.07 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.09 0.16 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 0 2 4 1 1 5 0 0 0 0 0 0 2 2 1 1 1 6
Bud Neck 0 0 0 0 0 0 0 0 1 1 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 2 5 6 2 16 19 13 18 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 7 27 13 20 21 28 28 39 19 16 10 14 31 41 67 33 30 11 0 4 1 7 8 10
Endoplasmic Reticulum 1 0 0 0 1 0 0 0 1 0 0 1 0 0 0 3 8 1 0 0 1 1 1 2
Endosome 0 0 2 0 1 2 0 1 0 0 0 0 0 0 3 2 1 1 4 5 1 0 2 5
Golgi 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 2 1 0 1 0 1 0
Mitochondria 3 47 0 2 28 15 27 17 186 83 87 127 2 0 3 5 4 3 0 11 1 6 5 8
Nucleus 491 431 298 364 403 490 575 545 615 514 349 318 245 262 338 185 161 111 478 448 298 184 198 278
Nuclear Periphery 0 5 1 4 5 7 9 10 12 8 3 4 1 2 9 5 4 2 1 1 1 1 1 8
Nucleolus 2 0 2 7 7 17 32 20 79 41 38 31 1 2 8 2 4 1 0 1 0 5 15 25
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 2 2 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 1 1 1 1 1 4 6 1 9 6 1 1 0 0 0 2 0 1 3
Vac/Vac Membrane 2 13 4 6 10 5 7 14 5 7 0 3 1 1 4 3 9 8 10 18 11 20 47 85
Unique Cell Count 506 481 317 390 444 538 642 614 730 590 402 392 278 313 420 226 205 130 502 497 328 234 291 445
Labelled Cell Count 507 523 320 406 479 570 686 651 939 691 505 529 282 320 440 241 222 140 502 497 328 234 291 445


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 6.7 8.6 8.0 6.4 6.8 6.4 6.9 5.3 5.8 5.4 4.9 7.9 8.2 8.0 8.7 11.0 11.8 8.9 9.5 9.0
Std Deviation (1e-4) 1.6 2.2 1.6 2.0 1.6 1.6 1.5 1.7 1.3 1.4 1.3 1.4 2.0 2.0 2.1 2.3 2.6 3.3 1.9 1.8 1.8
Intensity Change (Log2) – – – -0.1 -0.42 -0.34 -0.42 -0.31 -0.7 -0.57 -0.66 -0.81 -0.12 -0.08 -0.11 0.02 0.36 0.46 0.05 0.14 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.2 3.2 3.9 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.4 0.7 0.2 1.4 -1.3 -1.1 -1.2 -0.4 3.3 4.0 5.1 4.3 4.3 1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.6 3.0 3.7 3.0 9.9 7.0 8.8 11.2 0 0 0 0 0 0
Nucleus -0.4 -1.6 -1.5 -2.3 -2.6 -4.3 -3.2 -3.2 -5.1 -2.5 -4.1 -5.3 -4.5 -5.3 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.4 3.4 2.5 5.7 4.3 5.1 4.6 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.0 0 0 1.1 0 0 0 0 0 0 0 0 2.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.3638 8.1548 7.8509 7.0389 7.459 7.83 9.4628 9.8732 9.9037 8.8167 10.1737 9.9822 6.268 6.663 6.3881 6.5374 6.0499 6.396
Actin 0.0152 0.0005 0.0179 0.0001 0.0318 0.0001 0.0114 0 0.0002 0 0.0152 0.0001 0.0114 0 0.0039 0 0.0013 0
Bud 0.0015 0.0007 0.0008 0 0.0004 0.0001 0.0003 0 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0
Bud Neck 0.0024 0.0001 0.0011 0.0001 0.0012 0.0006 0.0008 0.0001 0.0001 0 0.0005 0.0003 0.0007 0 0.0014 0 0.0004 0.0003
Bud Periphery 0.0039 0.0009 0.0018 0.0002 0.0004 0.0001 0.0011 0.0001 0.0018 0 0.0001 0.0003 0.0003 0 0.0002 0 0.0002 0
Bud Site 0.0084 0.0022 0.0038 0.0001 0.0006 0.0001 0.0008 0.0001 0.0002 0 0.0003 0 0.0012 0 0.001 0 0.0004 0
Cell Periphery 0.0017 0 0.0006 0.0001 0.0001 0.0001 0.0004 0 0.0005 0 0.0001 0 0.0002 0 0.0002 0 0.0001 0
Cytoplasm 0.0067 0.0005 0.004 0 0.0032 0.0019 0.0012 0 0.0003 0 0.0028 0.0001 0.0021 0.0001 0.0011 0.0001 0.0011 0
Cytoplasmic Foci 0.0218 0.0018 0.0061 0.0006 0.0095 0.0005 0.005 0 0.0013 0 0.0025 0.0001 0.0044 0 0.0021 0 0.0029 0
Eisosomes 0.0009 0 0.0003 0 0.0001 0 0.0002 0 0 0 0.0002 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0061 0.0001 0.0035 0.0001 0.0026 0.0007 0.0038 0 0.0008 0 0.0012 0.0002 0.0022 0 0.0017 0 0.0008 0
Endosome 0.0259 0.0008 0.0124 0.0006 0.0601 0.0015 0.0134 0 0.0014 0 0.0027 0.0002 0.0057 0.0001 0.0046 0 0.0049 0
Golgi 0.0045 0.0002 0.0032 0.0005 0.0175 0.0006 0.0068 0 0.0002 0 0.0007 0 0.0011 0 0.0009 0 0.0045 0
Lipid Particles 0.0194 0.0003 0.0127 0.0201 0.0621 0.0023 0.0134 0 0.0017 0 0.0012 0.0002 0.0077 0 0.0019 0 0.0232 0
Mitochondria 0.0185 0.0004 0.0139 0.0033 0.0142 0.0047 0.0091 0.0002 0.0037 0 0.0007 0.0017 0.0009 0.0001 0.0014 0.0001 0.0164 0.0001
None 0.0152 0.0002 0.0036 0.0001 0.0005 0.0004 0.0016 0 0.0003 0 0.0103 0 0.0042 0 0.0006 0 0.0014 0
Nuclear Periphery 0.0226 0.001 0.0113 0.0022 0.0035 0.0041 0.0226 0.002 0.0043 0.0002 0.0233 0.0027 0.0108 0.0038 0.005 0.0041 0.0076 0.0005
Nucleolus 0.0197 0.017 0.012 0.0131 0.0125 0.0328 0.0112 0.0118 0.0096 0.0088 0.0234 0.0238 0.0124 0.0087 0.0062 0.009 0.0279 0.0215
Nucleus 0.7569 0.9714 0.8681 0.9549 0.7655 0.9441 0.8826 0.9854 0.9523 0.9906 0.8986 0.9677 0.9166 0.9863 0.962 0.9862 0.8851 0.9742
Peroxisomes 0.0062 0.0003 0.0081 0.0003 0.0087 0.0005 0.0042 0 0.0002 0 0.0009 0 0.0028 0 0.0003 0 0.0052 0
Punctate Nuclear 0.0273 0.0011 0.0072 0.0009 0.0016 0.0038 0.0038 0.0001 0.0109 0.0003 0.0134 0.0015 0.0118 0.0003 0.0042 0.0002 0.0149 0.0032
Vacuole 0.0112 0.0004 0.005 0.0009 0.0017 0.0006 0.0034 0.0001 0.0061 0 0.001 0.0004 0.0025 0.0003 0.0007 0 0.0011 0.0001
Vacuole Periphery 0.004 0.0001 0.0026 0.0015 0.0022 0.0003 0.0029 0.0001 0.0038 0 0.0009 0.0006 0.0008 0.0002 0.0004 0.0001 0.0006 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.149 7.882 13.3687 8.8833 6.5325 30.2135 8.2541 8.8367 12.2337 8.2369
Translational Efficiency 2.8254 1.4985 0.8431 1.4702 1.6287 0.379 1.2913 1.3868 0.9225 1.3044

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
212 326 2598 1405 2136 1140 1886 1945 2348 1466 4484 3350

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 840.37 839.54 1061.64 1002.20 832.97 1042.95 1042.28 1069.90 833.64 997.72 1053.50 1041.51
Standard Deviation 121.69 213.32 168.28 172.72 128.31 163.78 163.68 178.10 127.75 195.28 166.64 179.01
Intensity Change Log 2 — -0.001426 0.337198 0.254074 — 0.324334 0.323406 0.361140 — 0.169912 0.330349 0.308363

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001082 0.000812 0.000406 0.000749 0.000173 0.000378 0.000349 0.000423 0.000255 0.000474 0.000382 0.000560
Bud Neck 0.001991 0.009665 0.016498 0.018997 0.004302 0.005570 0.009308 0.020286 0.004094 0.006480 0.013474 0.019745
Bud Site 0.000291 0.002732 0.001062 0.003124 0.000449 0.001594 0.000451 0.001245 0.000434 0.001847 0.000805 0.002033
Cell Periphery 0.000069 0.000706 0.000143 0.000188 0.000226 0.000269 0.000111 0.000123 0.000211 0.000366 0.000129 0.000150
Cytoplasm 0.000261 0.004276 0.000470 0.003528 0.000416 0.001034 0.000278 0.001101 0.000402 0.001755 0.000389 0.002119
Cytoplasmic Foci 0.000194 0.000233 0.000034 0.001539 0.000055 0.001147 0.000033 0.000102 0.000068 0.000944 0.000034 0.000705
Eisosomes 0.000019 0.000037 0.000047 0.000031 0.000034 0.000033 0.000054 0.000035 0.000033 0.000034 0.000050 0.000033
Endoplasmic Reticulum 0.005145 0.008265 0.003403 0.004652 0.007361 0.009580 0.003337 0.004238 0.007161 0.009287 0.003375 0.004412
Endosome 0.000107 0.001396 0.000132 0.002409 0.000219 0.001493 0.000110 0.000315 0.000209 0.001471 0.000123 0.001193
Golgi 0.000137 0.000344 0.000105 0.002696 0.000026 0.000908 0.000057 0.000522 0.000036 0.000782 0.000085 0.001434
Lipid Particles 0.000138 0.000106 0.000014 0.000410 0.000039 0.000235 0.000009 0.000018 0.000048 0.000206 0.000012 0.000182
Mitochondria 0.000480 0.000999 0.001341 0.002796 0.000404 0.003180 0.000721 0.001542 0.000411 0.002695 0.001080 0.002068
Mitotic Spindle 0.002461 0.000679 0.000779 0.013475 0.000615 0.000873 0.000217 0.002773 0.000781 0.000830 0.000543 0.007261
None 0.001294 0.015088 0.000967 0.005601 0.001577 0.002163 0.000503 0.001195 0.001552 0.005037 0.000772 0.003043
Nuclear Periphery 0.000100 0.000302 0.000598 0.001507 0.000333 0.000980 0.000167 0.000584 0.000312 0.000829 0.000417 0.000971
Nuclear Periphery Foci 0.000006 0.000458 0.000342 0.000919 0.000288 0.000808 0.000019 0.000061 0.000263 0.000730 0.000206 0.000421
Nucleolus 0.004216 0.008207 0.002212 0.004269 0.006522 0.008619 0.002080 0.001956 0.006314 0.008527 0.002156 0.002926
Nucleus 0.981085* 0.942905* 0.970392* 0.924420* 0.975786* 0.956812* 0.981273* 0.961457* 0.976264* 0.953720* 0.974969* 0.945923*
Peroxisomes 0.000616 0.000488 0.000057 0.000209 0.000067 0.000648 0.000057 0.000056 0.000116 0.000612 0.000057 0.000120
Vacuole 0.000259 0.001997 0.000759 0.006236 0.000871 0.002228 0.000680 0.001491 0.000816 0.002177 0.000726 0.003481
Vacuole Periphery 0.000049 0.000305 0.000239 0.002243 0.000236 0.001449 0.000188 0.000479 0.000219 0.001194 0.000217 0.001219

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.26 0.73 0.19 -0.44 -6.26 -6.19 -11.77 -7.83 -1.86 -2.88 -2.53 -1.87 -4.56 -3.15 -6.25
Bud Neck -5.25 -15.44 -13.69 -6.29 -2.70 -1.72 -9.68 -13.32 -11.65 -9.36 -3.53 -15.83 -17.87 -14.15 -7.40
Bud Site -4.06 -4.14 -5.46 -0.62 -3.86 -2.16 -0.23 -4.21 0.33 -4.91 -3.25 -2.47 -6.26 -0.72 -5.04
Cell Periphery -2.95 -3.15 -2.27 2.50 -0.59 -0.44 2.56 2.39 1.69 -0.43 -1.65 2.09 1.77 2.68 -0.48
Cytoplasm -3.65 -1.66 -5.64 0.34 -5.34 -1.94 0.44 -3.70 -1.26 -3.83 -3.85 -0.15 -6.41 -1.67 -6.56
Cytoplasmic Foci -0.40 0.86 -2.38 -2.14 -3.20 -2.48 0.62 -0.84 2.36 -1.36 -2.57 1.02 -3.08 1.19 -3.42
Eisosomes -4.21 -8.75 -5.80 1.01 4.95 0.23 -3.83 0.26 -0.00 4.66 -0.38 -4.54 -0.16 0.35 6.53
Endoplasmic Reticulum -3.43 2.97 0.33 4.27 -5.52 -3.17 11.43 8.37 8.48 -3.26 -3.59 11.37 7.95 9.18 -5.59
Endosome -1.37 -0.67 -3.43 -0.39 -3.37 -3.02 1.65 -0.58 2.78 -1.58 -3.24 1.67 -3.21 1.22 -3.68
Golgi -1.06 0.25 -2.17 -1.82 -2.24 -2.04 -0.71 -1.48 0.87 -1.37 -2.23 -1.03 -2.72 -0.53 -2.59
Lipid Particles 0.16 0.99 -1.10 -1.50 -2.16 -2.47 1.87 0.90 2.72 -2.27 -2.53 1.92 -1.67 0.51 -2.27
Mitochondria -2.08 -3.94 -6.54 -5.53 -3.93 -2.77 -3.88 -6.05 1.52 -4.59 -2.93 -6.49 -9.55 0.71 -4.96
Mitotic Spindle 0.69 0.67 -3.10 -5.09 -5.10 -0.39 0.98 -2.88 -2.68 -3.39 -0.06 0.54 -5.54 -5.64 -6.01
None -3.15 0.70 -2.84 2.23 -3.25 -1.07 3.19 0.29 1.51 -5.90 -3.23 2.50 -2.29 1.81 -4.10
Nuclear Periphery -3.55 -2.99 -5.08 -4.26 -2.48 -2.38 2.75 -4.64 1.21 -10.06 -2.42 -1.26 -5.45 -0.74 -3.37
Nuclear Periphery Foci -2.77 -1.75 -3.35 -1.45 -1.87 -0.72 1.10 0.92 1.31 -2.13 -0.81 0.39 -0.48 0.60 -1.54
Nucleolus -3.24 2.41 -1.81 1.38 -3.44 -2.06 8.53 5.35 5.58 -1.89 -2.60 7.88 2.91 4.68 -3.66
Nucleus 4.55 2.59 8.74 2.39 9.17 4.59 -2.86 7.11 0.36 9.47 6.18 1.74 12.28 2.95 11.96
Peroxisomes 0.08 1.14 0.81 1.23 -3.91 -3.11 1.16 -0.12 3.08 -0.68 -3.13 1.23 -0.35 3.12 -3.62
Vacuole -1.74 -4.98 -5.75 -2.92 -5.16 -2.57 0.66 -2.25 0.95 -3.20 -2.84 0.15 -5.44 -2.18 -5.93
Vacuole Periphery -2.68 -1.98 -3.79 -3.09 -3.25 -2.58 0.40 -0.94 1.98 -1.07 -2.66 0.02 -3.29 0.59 -3.21
Standard name
Human Ortholog
Description mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Spp381

Spp381


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spp381-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available