Standard name
Human Ortholog
Description Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.81 0.94 0.97 0.88 0.73 0.68 0.67 0.58 0.57 0.38 0.28 0.97 0.99 0.99 0.95 0.95 0.46 0.56 0.56 0.8 0.73 0.65
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0.13 0.14 0.45 0.46 0.4 0.64 0.65 0.75 0.84 0 0 0 0 0 0.07 0.06 0.2 0.05 0 0.06
Nucleus 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.11 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.07 0 0 0 0
Vac/Vac Membrane 0.13 0.13 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07 0.09 0.05 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 12 0 3 8
Bud 3 1 3 0 2 10 7 9 10 11 6 4 0 2 0 0 0 6 20 8 2 5 10
Bud Neck 0 1 1 1 3 3 4 8 1 3 0 2 0 0 0 2 3 0 2 6 0 1 4
Bud Site 0 0 0 0 0 2 0 0 5 2 2 2 0 0 0 0 0
Cell Periphery 2 1 1 2 1 0 0 2 0 0 0 2 1 0 1 11 6 0 2 2 1 1 3
Cytoplasm 102 366 395 222 263 310 351 298 240 279 78 74 266 338 364 525 507 84 260 251 136 221 217
Endoplasmic Reticulum 0 0 1 0 2 0 0 1 0 0 5 6 2 0 4 49 42 1 1 4 1 3 5
Endosome 3 3 1 0 3 2 0 0 1 1 0 0 0 3 0 6 5 1 4 14 0 3 4
Golgi 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1 3 0
Mitochondria 24 11 13 29 43 190 235 179 265 317 152 221 0 2 0 10 10 13 28 89 8 13 21
Nucleus 17 39 3 0 2 2 3 2 3 2 1 1 4 0 0 1 2 29 51 5 2 10 6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 1 1 0 1 0 2 1 1 1 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 2 0 1 1 0 0 0
SpindlePole 1 0 2 0 1 5 8 13 7 8 2 5 0 0 0 4 1 22 32 12 4 12 10
Vac/Vac Membrane 20 60 19 1 3 5 8 6 5 5 1 3 5 0 1 35 22 13 43 20 6 14 28
Unique Cell Count 153 450 419 230 298 426 514 446 417 491 204 264 274 342 369 553 534 182 465 446 171 304 333
Labelled Cell Count 173 482 440 255 326 531 616 519 537 631 248 322 279 345 370 645 601 182 465 446 171 304 333


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.3 4.0 3.5 3.8 3.2 2.6 3.0 2.9 2.5 2.7 2.5 4.5 4.2 4.4 4.8 5.9 5.4 4.3 4.5 5.0
Std Deviation (1e-4) 1.4 0.8 1.6 1.5 1.8 1.4 0.7 1.0 1.1 0.7 0.6 0.7 1.4 1.2 1.6 1.9 1.9 2.0 1.9 1.9 1.5
Intensity Change (Log2) -0.18 -0.07 -0.31 -0.63 -0.43 -0.47 -0.66 -0.53 -0.65 0.16 0.07 0.14 0.28 0.57 0.45 0.1 0.17 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 1.2 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1693 1.2017 1.3169 1.1106 0.343 1.8065 2.9311 2.3238 2.4789 2.6638 2.0554 2.406 4.2038 2.9067 3.1727 2.9359 3.3377 3.1537
Actin 0.1846 0.0009 0.0001 0.0011 0.0041 0.0016 0.0059 0.0029 0.0287 0.0008 0.003 0.0052 0.0109 0.0005 0.0146 0.0004 0.003 0.0019
Bud 0.007 0.0004 0 0.0062 0.0018 0.0058 0.0004 0.017 0.0156 0.0002 0.0002 0.0018 0.0005 0.0066 0.0036 0.0004 0.007 0.0015
Bud Neck 0.0043 0.0001 0 0.0001 0.0002 0.0067 0.0008 0.0008 0.0006 0 0.0001 0.0015 0.0088 0.0005 0.0026 0.0005 0.0001 0.0039
Bud Periphery 0.0344 0.0009 0.0001 0.0044 0.0038 0.0072 0.0005 0.0147 0.0044 0.0007 0.0003 0.001 0.0008 0.0079 0.0087 0.0004 0.0104 0.0019
Bud Site 0.0184 0.0013 0.0001 0.0019 0.001 0.0093 0.0027 0.0263 0.0112 0 0.0006 0.0003 0.0067 0.0151 0.0092 0.0007 0.0009 0.0006
Cell Periphery 0.0052 0.0003 0.0002 0.0001 0.0001 0.0003 0.0002 0.001 0.0004 0.0001 0.0001 0.0001 0.0005 0.0004 0.0007 0.0001 0.0003 0.0003
Cytoplasm 0.1914 0.0986 0.0315 0.0896 0.017 0.1474 0.2142 0.1554 0.1082 0.0399 0.1542 0.2222 0.1769 0.0819 0.0854 0.0933 0.0597 0.2033
Cytoplasmic Foci 0.0198 0.0024 0.0018 0.0049 0.0083 0.0101 0.0607 0.0051 0.0171 0.0036 0.0049 0.0137 0.0326 0.0069 0.0106 0.0425 0.0075 0.0136
Eisosomes 0.0019 0.0001 0.0002 0.0004 0.0004 0.0001 0.0004 0.0003 0.0003 0.0002 0.0001 0.0001 0.0004 0.0002 0.0003 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0216 0.0014 0.0004 0.0011 0.0003 0.002 0.0043 0.0038 0.0019 0.0006 0.0009 0.0021 0.0102 0.0023 0.0044 0.0013 0.001 0.0022
Endosome 0.0236 0.0005 0.0002 0.0005 0.0002 0.0015 0.0296 0.0077 0.0035 0.0004 0.0179 0.0051 0.0223 0.0015 0.0142 0.0161 0.0018 0.0027
Golgi 0.011 0.0001 0 0.0002 0.0002 0.0002 0.0158 0.0022 0.0029 0.0001 0.0224 0.004 0.0036 0.0001 0.0075 0.0016 0.0047 0.0003
Lipid Particles 0.0131 0.0002 0.0002 0.0003 0.0007 0.0004 0.0171 0.0005 0.0084 0.0003 0.0009 0.0026 0.0156 0.0003 0.0041 0.0019 0.0097 0.0008
Mitochondria 0.0299 0.0002 0.0002 0.0009 0.001 0.0011 0.0083 0.0036 0.0129 0.0004 0.0138 0.0022 0.0018 0.0007 0.0043 0.0005 0.0289 0.0009
None 0.3011 0.8906 0.9638 0.8622 0.9449 0.5756 0.4482 0.7307 0.7491 0.9475 0.778 0.6439 0.5325 0.8468 0.795 0.8285 0.8305 0.6766
Nuclear Periphery 0.0171 0.0004 0.0002 0.0024 0.0007 0.0039 0.0091 0.0027 0.0009 0.0007 0.0003 0.0067 0.0097 0.002 0.0083 0.0006 0.0002 0.003
Nucleolus 0.007 0.0001 0.0001 0.001 0.0012 0.003 0.0017 0.0009 0.0007 0.0003 0.0001 0.0013 0.0014 0.0009 0.0006 0.0004 0.0001 0.0011
Nucleus 0.0117 0.0005 0.0002 0.0058 0.0016 0.199 0.1373 0.0162 0.0072 0.0005 0.0003 0.0642 0.1401 0.0169 0.0118 0.0009 0.0003 0.0714
Peroxisomes 0.0107 0.0001 0.0001 0.0005 0.0014 0.0002 0.03 0.0004 0.0227 0.0002 0.0005 0.0031 0.0099 0.0004 0.0043 0.0017 0.033 0.0008
Punctate Nuclear 0.0298 0.0003 0.0003 0.0157 0.0106 0.0213 0.0095 0.0037 0.0015 0.0027 0.0003 0.0173 0.012 0.0053 0.0019 0.0054 0.0003 0.011
Vacuole 0.0448 0.0005 0.0002 0.0005 0.0002 0.0029 0.0027 0.003 0.0013 0.0006 0.0007 0.0012 0.0023 0.0026 0.0056 0.0022 0.0003 0.0019
Vacuole Periphery 0.0115 0.0001 0 0.0002 0.0001 0.0004 0.0007 0.001 0.0004 0.0003 0.0005 0.0005 0.0007 0.0003 0.0023 0.0002 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.7152 14.3473 39.9237 153.9837 119.6512 15.3723 15.111 16.9301 85.2862 155.6867
Translational Efficiency 0.1324 0.1742 0.2881 0.2099 0.3775 0.1662 0.1473 0.1551 0.2286 0.3435

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1186 1454 282 1660 1183 2062 245 3050 2369 3516 527 4710

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 629.18 688.34 867.28 884.58 623.88 720.09 930.68 987.58 626.53 706.96 896.75 951.28
Standard Deviation 65.48 85.65 119.44 153.76 72.74 104.81 119.42 394.01 69.25 98.59 123.54 333.59
Intensity Change Log 2 0.129649 0.463025 0.491520 0.206909 0.577017 0.662629 0.168632 0.520905 0.579248

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000048 0.000380 0.000385 0.000833 0.000168 0.000356 0.000643 0.000782 0.000108 0.000366 0.000505 0.000800
Bud Neck 0.010311 0.027912 0.003054 0.022081 0.010718 0.037548 0.008183 0.023920 0.010514 0.033563 0.005438 0.023272
Bud Site 0.002686 0.014951 0.001869 0.034174 0.003397 0.017101 0.010523 0.036993 0.003041 0.016212 0.005892 0.035999
Cell Periphery 0.000161 0.000186 0.000176 0.000342 0.000166 0.000219 0.000275 0.000559 0.000163 0.000205 0.000222 0.000483
Cytoplasm 0.537447* 0.396285* 0.120134* 0.371780* 0.440113* 0.441059* 0.188299* 0.516101* 0.488842* 0.422543* 0.151824* 0.465236*
Cytoplasmic Foci 0.181026* 0.170640 0.013129 0.012078 0.180131* 0.145552 0.008873 0.003951 0.180579* 0.155927 0.011150 0.006816
Eisosomes 0.000158 0.000103 0.000071 0.000036 0.000139 0.000085 0.000082 0.000017 0.000149 0.000092 0.000076 0.000024
Endoplasmic Reticulum 0.000399 0.001177 0.004823 0.003296 0.000675 0.000955 0.007255 0.000816 0.000537 0.001046 0.005954 0.001690
Endosome 0.006595 0.014518 0.002681 0.005269 0.007606 0.015131 0.003318 0.002037 0.007100 0.014877 0.002977 0.003176
Golgi 0.001171 0.004267 0.000072 0.002050 0.001683 0.003592 0.000115 0.001449 0.001427 0.003871 0.000092 0.001661
Lipid Particles 0.007350 0.006376 0.002329 0.001022 0.010390 0.005475 0.001986 0.000328 0.008868 0.005847 0.002170 0.000572
Mitochondria 0.002974 0.012447 0.001184 0.004023 0.005066 0.015640 0.000959 0.006098 0.004018 0.014319 0.001079 0.005366
Mitotic Spindle 0.000768 0.005655 0.005629 0.076023 0.000867 0.013787 0.019566 0.062135 0.000818 0.010424 0.012108 0.067030
None 0.005108 0.008990 0.005502 0.010452 0.005983 0.006960 0.006801 0.014377 0.005545 0.007799 0.006106 0.012994
Nuclear Periphery 0.000374 0.000636 0.001728 0.002000 0.000765 0.000888 0.002051 0.001231 0.000569 0.000784 0.001878 0.001502
Nuclear Periphery Foci 0.000214 0.000445 0.000744 0.002082 0.000314 0.000479 0.001655 0.000483 0.000264 0.000465 0.001168 0.001047
Nucleolus 0.000996 0.004505 0.001376 0.001632 0.002302 0.003809 0.001513 0.001266 0.001648 0.004096 0.001439 0.001395
Nucleus 0.204044* 0.253333* 0.781231* 0.387264* 0.290911* 0.225629* 0.628145* 0.293512* 0.247422* 0.237085* 0.710062* 0.326554*
Peroxisomes 0.002130 0.012705 0.000324 0.001037 0.004721 0.010839 0.000299 0.000384 0.003424 0.011611 0.000312 0.000614
Vacuole 0.035534 0.062576 0.053255 0.058380 0.032813 0.052943 0.109165 0.031892 0.034175 0.056927 0.079247 0.041228
Vacuole Periphery 0.000508 0.001913 0.000306 0.004144 0.001071 0.001954 0.000295 0.001668 0.000789 0.001937 0.000301 0.002540

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.52 -9.38 -11.28 -4.60 -3.36 -1.86 -4.80 -2.85 -1.24 1.35 -4.38 -8.25 -6.53 -2.79 -0.24
Bud Neck -8.15 5.60 -6.51 2.70 -13.42 -11.55 2.79 -9.67 5.31 -11.67 -14.17 5.68 -11.93 5.75 -18.87
Bud Site -6.94 0.69 -9.83 -2.93 -10.57 -7.74 -1.53 -11.15 -3.34 -6.14 -10.63 -1.18 -15.26 -4.51 -11.84
Cell Periphery -1.56 -0.54 -3.21 -1.81 -1.83 -1.02 -1.80 -2.46 -0.90 -1.16 -1.35 -1.67 -3.23 -1.33 -1.95
Cytoplasm 14.01 35.95 27.32 12.16 -14.71 -0.18 18.63 4.52 5.18 -16.93 9.27 37.32 19.80 10.42 -24.60
Cytoplasmic Foci 1.24 30.41 33.04 29.48 0.66 5.03 29.74 31.25 34.19 2.80 4.86 42.32 45.75 45.22 2.72
Eisosomes 5.66 6.06 12.15 9.20 4.55 7.63 3.10 17.55 17.11 9.96 9.51 6.62 21.30 19.10 11.17
Endoplasmic Reticulum -8.07 -10.40 -21.83 -17.11 1.98 -2.44 -8.37 -7.17 -4.30 7.58 -6.73 -12.73 -21.71 -15.30 8.57
Endosome -6.93 5.21 4.17 10.24 -1.51 -6.66 4.92 9.54 14.33 5.32 -9.65 7.22 10.58 17.84 3.70
Golgi -3.83 3.06 1.09 4.72 -3.20 -2.73 3.17 2.03 5.91 -2.93 -4.75 4.36 2.31 7.49 -4.24
Lipid Particles 0.95 5.63 7.47 8.77 3.26 5.30 9.72 12.74 9.44 6.97 4.56 10.85 14.69 12.98 7.87
Mitochondria -5.67 2.02 0.59 6.90 -2.64 -6.12 4.38 1.43 8.04 -5.37 -8.47 4.51 1.20 10.40 -5.55
Mitotic Spindle -3.65 -0.96 -14.54 -13.20 -11.38 -7.16 -2.26 -19.50 -13.49 -9.22 -8.00 -2.34 -24.28 -19.03 -15.08
None -2.92 0.60 -4.27 -0.13 -5.74 -0.83 1.56 -8.85 -9.88 -12.46 -2.65 1.54 -10.47 -7.59 -14.18
Nuclear Periphery -3.75 -11.73 -27.03 -23.96 -10.58 -0.45 -10.37 -14.46 -5.59 0.77 -1.29 -15.16 -27.06 -12.11 -5.10
Nuclear Periphery Foci -5.22 -4.74 -7.48 -7.12 -6.31 -1.55 -6.02 -3.24 -1.71 4.58 -3.28 -7.34 -8.13 -7.20 -2.26
Nucleolus -7.12 -1.65 -5.19 1.54 -3.95 -2.37 2.31 -3.07 -1.17 -6.52 -6.35 1.37 -5.74 -0.04 -7.60
Nucleus -5.36 -34.25 -22.83 -16.12 20.08 7.08 -12.81 -11.09 -20.91 8.42 1.68 -30.29 -23.94 -26.13 19.50
Peroxisomes -9.57 7.67 5.61 10.97 -3.32 -6.42 8.29 8.09 14.20 -1.57 -11.72 10.51 9.82 17.78 -3.14
Vacuole -8.59 -4.68 -19.14 -12.63 -5.84 -7.17 -9.71 -10.15 -3.42 7.86 -11.06 -10.33 -20.46 -10.95 4.41
Vacuole Periphery -8.14 2.89 -1.37 1.14 -1.77 -2.01 1.92 0.77 6.39 -3.40 -4.84 2.38 -0.44 4.35 -3.03
Standard name
Human Ortholog
Description Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box
Localization
Cell Percentages cytoplasm (26%), nucleus (7%), mixed (39%)
Cell Cycle Regulation Yes
cytoplasm - S/G2 (2.6e-11)
cytoplasm - MA (8.8e-12)
nucleus - T/G1 (1.6e-14)
Subcompartmental Group N/A

Amn1

Amn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Amn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available