Standard name
Human Ortholog
Description E1 beta subunit of the pyruvate dehydrogenase (PDH) complex; PDH is an evolutionarily conserved multi-protein complex found in mitochondria
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.26 0.08 – 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0.06 – 0 0 0 0.07 0.06 0.05
Golgi 0 0 0.08 0.05 0.11 0 0 0 0 0 0 0 0.09 0.1 0.19 0.21 0.22 – 0 0.13 0.06 0.08 0.11 0.09
Mitochondria 0.92 0.9 0.79 0.83 0.83 0.85 0.89 0.85 0.93 0.9 0.93 0.9 0.79 0.72 0.62 0.05 0.17 – 0.78 0.71 0.73 0.6 0.52 0.51
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
Nucleolus 0.11 0.17 0.08 0.05 0.09 0.15 0.19 0.2 0.19 0.19 0.16 0.18 0.09 0.12 0.1 0.16 0.31 – 0 0 0 0.05 0 0.06
Peroxisomes 0 0 0.11 0.15 0.06 0 0 0 0 0 0 0 0.13 0.19 0.1 0.32 0.08 – 0.08 0 0.08 0.09 0.16 0.14
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0.11 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 5 7 7 3 5 7 2 5 1 2 5 0 0 5 3 – 2 2 9 4 12 7
Bud 1 0 3 9 13 9 4 9 2 1 9 3 4 0 1 1 1 – 0 0 0 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 4
Bud Site 0 0 1 2 0 1 3 0 0 1 1 0 1 0 0 0 0 – – – – – – –
Cell Periphery 0 0 3 3 0 1 2 1 0 1 1 1 1 3 1 1 0 – 0 0 0 0 0 0
Cytoplasm 2 2 5 5 4 2 5 12 5 1 4 1 1 1 3 1 3 – 0 0 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 1 2 2 0 1 0 0 1 0 0 0 3 – 0 0 0 0 0 0
Endosome 1 0 6 3 6 2 0 1 0 0 1 1 8 6 13 0 2 – 2 0 7 7 14 11
Golgi 0 1 17 13 36 8 4 6 7 7 0 8 13 16 32 4 8 – 2 8 13 9 26 19
Mitochondria 68 38 162 221 276 281 425 429 287 376 297 259 120 112 102 1 6 – 69 47 163 68 124 115
Nucleus 0 0 2 1 0 1 2 0 1 2 1 2 1 0 0 0 0 – 0 0 0 0 0 1
Nuclear Periphery 5 0 3 5 9 9 16 12 8 9 7 3 4 3 7 1 1 – 0 0 0 0 3 3
Nucleolus 8 7 16 12 30 51 91 101 57 81 52 52 14 18 16 3 11 – 1 1 1 5 6 13
Peroxisomes 2 0 22 39 20 10 7 12 3 1 2 1 20 29 17 6 3 – 7 1 18 9 37 32
SpindlePole 1 0 3 4 3 4 0 0 0 1 0 0 4 5 8 0 0 – 0 0 2 3 3 4
Vac/Vac Membrane 1 0 12 2 10 13 13 12 11 14 4 11 7 7 13 0 4 – 0 2 3 1 4 6
Unique Cell Count 74 42 205 265 332 330 476 503 308 420 320 287 151 156 165 19 36 89 67 225 113 240 224
Labelled Cell Count 89 49 260 326 414 396 579 604 383 501 380 344 204 200 213 23 45 89 67 225 113 240 224


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.5 20.6 16.3 16.7 19.7 22.9 26.1 28.3 27.3 31.7 28.7 32.2 14.8 15.1 16.0 25.3 26.2 – 13.0 14.7 14.4
Std Deviation (1e-4) 4.7 7.1 5.4 5.7 6.5 9.2 9.7 9.9 10.3 11.7 10.6 9.5 5.5 5.2 6.7 10.0 11.6 – 4.4 6.0 5.7
Intensity Change (Log2) – – – 0.03 0.27 0.49 0.68 0.79 0.74 0.96 0.81 0.98 -0.14 -0.11 -0.02 0.64 0.68 – -0.32 -0.15 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 1.3 1.6 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 0 0 0 0 0 0 1.1 0.5 2.1 0 0 –
Golgi -1.5 1.0 -3.1 -5.2 -4.8 -3.2 -4.0 -5.2 -2.7 0.1 0.6 3.1 0 0 –
Mitochondria 1.2 1.2 1.8 3.6 2.0 4.8 3.6 4.6 3.5 0.1 -1.6 -3.6 -6.9 -7.5 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 1.4 0 0 0 0 0 0 0 0 0 0 –
Nucleolus -1.5 0.5 2.6 3.7 4.0 3.4 3.7 2.8 3.3 0.5 1.2 0.6 0 0 –
Peroxisomes 1.3 -2.0 -3.7 -5.5 -4.7 -5.0 -6.5 -5.4 -5.4 0.7 2.1 -0.1 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole -3.2 -1.6 -1.0 -2.0 -2.3 -1.2 -1.5 -3.0 -1.0 -0.5 -0.6 0.8 0 0 –