Standard name
Human Ortholog
Description Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene; YBR241C has a paralog, VPS73, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.06 0.13 0.12 0.43 0.5 0.46 0.36 0.42 0.45 0.47 0.54 0.46 0.18 0.24 0.11 0.25 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0.11 0.06 0.08 0.08 0.1 0.08 0.05 0.05 0.05 0 0 0 0.12 – – 0 0 0 0 0 0
Endosome 0.08 0 0.17 0.11 0.09 0 0 0 0 0 0 0 0 0.06 0.05 0 – – 0.08 0 0.12 0.05 0.08 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.11 0.17 0.13 0.27 0.27 0.27 0.25 0 0 0 0 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0.06 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.91 0.87 0.82 0.39 0.49 0.41 0.45 0.39 0.29 0.3 0.26 0.33 0.81 0.79 0.91 0.67 – – 0.83 0.86 0.77 0.83 0.81 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 3 10 3 8 2 2 2 1 0 0 – – 0 0 0 0 1 0
Bud 0 0 0 0 0 0 5 1 4 2 3 4 0 1 0 0 – – 0 1 3 2 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 1 1 4 11 16 14 10 11 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 7 38 51 99 182 223 240 325 196 205 267 223 33 30 17 6 – – 0 6 1 1 1 3
Endoplasmic Reticulum 2 3 6 26 22 39 53 76 37 20 23 22 4 2 3 3 – – 0 1 3 0 2 1
Endosome 10 5 73 25 31 18 27 21 7 6 3 10 4 8 8 0 – – 11 13 54 7 21 23
Golgi 1 1 4 4 7 12 28 29 8 5 5 9 0 2 2 1 – – 0 2 2 0 1 0
Mitochondria 4 7 13 13 6 51 111 102 118 116 134 122 0 0 0 1 – – 3 3 4 1 2 3
Nucleus 2 10 5 1 2 5 5 7 3 2 5 0 9 5 4 0 – – 2 3 2 5 5 7
Nuclear Periphery 3 4 8 3 1 2 8 27 9 4 2 5 3 0 2 0 – – 3 1 26 5 6 4
Nucleolus 3 2 13 1 0 1 4 3 1 3 1 1 0 1 1 0 – – 0 1 1 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 4 0 0 0 0 0 0 0 1 0 0 0 0 0 – – 0 0 1 0 0 1
Vac/Vac Membrane 108 250 354 91 177 200 298 306 127 131 131 163 149 100 144 16 – – 111 264 354 130 208 192
Unique Cell Count 119 289 434 231 362 484 669 781 437 434 499 487 184 127 159 24 135 307 460 156 257 242
Labelled Cell Count 140 320 531 264 430 552 786 918 529 516 587 572 204 150 181 27 135 307 460 156 257 242


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 8.7 7.7 7.8 9.3 8.9 9.2 10.0 9.4 9.4 9.5 10.1 9.1 10.1 11.3 10.0 – – 7.8 9.1 10.0
Std Deviation (1e-4) 1.7 1.8 1.7 1.8 2.3 2.3 2.3 2.6 2.8 2.9 3.0 3.3 2.3 2.5 3.3 3.0 – – 1.7 1.9 2.3
Intensity Change (Log2) – – – 0.01 0.26 0.21 0.25 0.37 0.28 0.28 0.29 0.38 0.23 0.38 0.54 0.36 – – 0.0 0.23 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 4.0 3.8 0 3.1 0 0 0 0 – –
Cytoplasm 9.1 11.9 11.3 8.9 10.8 10.8 11.5 13.4 11.3 2.1 3.3 -0.4 0 – –
Endoplasmic Reticulum 5.7 3.6 4.7 4.7 5.6 4.8 2.8 2.8 2.8 0 0 0 0 – –
Endosome -2.1 -3.4 -6.6 -7.2 -8.8 -7.8 -7.9 -9.0 -7.8 -5.0 -3.0 -3.7 0 – –
Golgi 0 1.2 1.8 3.2 2.9 1.2 0 0 1.2 0 0 0 0 – –
Mitochondria 1.7 -1.2 4.5 7.0 5.7 9.9 9.8 10.0 9.4 0 0 0 0 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 -0.9 1.6 0.2 -1.2 0 -1.0 0 0 0 0 – –
Nucleolus 0 -3.3 -3.4 -3.2 -3.8 -3.2 -2.5 -3.5 -3.5 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Vacuole -11.0 -9.7 -12.4 -12.2 -14.2 -15.6 -15.2 -16.9 -14.7 -0.2 -0.7 2.6 -1.8 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0057 0 0.0012 0.0383 0.0001 0.0012 0.0081 0 0.0047 0 0 0.0001 – – – – – –
Bud 0.0005 0.0008 0.0033 0.0006 0.0004 0.0017 0.0008 0.0029 0.001 0.0002 0.0004 0.0003 – – – – – –
Bud Neck 0.0038 0.0005 0.0008 0.0006 0.0004 0.0012 0.001 0.0007 0.0021 0.0004 0.0003 0.0006 – – – – – –
Bud Periphery 0.001 0.0011 0.0075 0.0007 0.0006 0.0027 0.0018 0.0037 0.0019 0.0003 0.0008 0.0006 – – – – – –
Bud Site 0.0014 0.0024 0.0044 0.0018 0.0002 0.0011 0.0027 0.0061 0.0027 0.0009 0.0005 0.0005 – – – – – –
Cell Periphery 0.0007 0.0008 0.0049 0.0003 0.0006 0.0005 0.0011 0.0008 0.0012 0.0004 0.0005 0.0007 – – – – – –
Cytoplasm 0.021 0.0435 0.0325 0.0219 0.0139 0.0292 0.0192 0.0175 0.0209 0.006 0.0178 0.019 – – – – – –
Cytoplasmic Foci 0.0127 0.0066 0.0085 0.0099 0.007 0.0068 0.012 0.0083 0.0163 0.0109 0.0087 0.0088 – – – – – –
Eisosomes 0.0001 0 0.0001 0.0002 0 0 0.0008 0 0 0 0 0 – – – – – –
Endoplasmic Reticulum 0.0065 0.0052 0.0056 0.0026 0.0016 0.0049 0.0055 0.0097 0.0043 0.001 0.0023 0.0032 – – – – – –
Endosome 0.1943 0.1378 0.1646 0.1106 0.1926 0.1563 0.2153 0.1456 0.249 0.3025 0.2013 0.2123 – – – – – –
Golgi 0.01 0.0019 0.0024 0.0114 0.0032 0.0043 0.0077 0.0022 0.0083 0.0041 0.0081 0.0039 – – – – – –
Lipid Particles 0.0091 0.0009 0.0036 0.0092 0.0013 0.0052 0.0093 0.0018 0.0037 0.0037 0.0054 0.0039 – – – – – –
Mitochondria 0.0116 0.0025 0.0062 0.0022 0.0042 0.0052 0.0182 0.0048 0.0117 0.0024 0.0061 0.0106 – – – – – –
None 0.0127 0.0025 0.0121 0.0136 0.0004 0.0009 0.0152 0.0026 0.0088 0.0005 0.0401 0.0126 – – – – – –
Nuclear Periphery 0.0245 0.0155 0.012 0.011 0.0059 0.0141 0.0097 0.0127 0.0139 0.0038 0.004 0.0185 – – – – – –
Nucleolus 0.0031 0.0017 0.0019 0.0011 0.0006 0.0015 0.002 0.0018 0.0033 0.0016 0.0007 0.0013 – – – – – –
Nucleus 0.0105 0.0136 0.0056 0.0036 0.0019 0.0135 0.0107 0.0046 0.01 0.0018 0.0027 0.0043 – – – – – –
Peroxisomes 0.0068 0.0001 0.0005 0.0048 0.0002 0.0061 0.0088 0.0006 0.0019 0.0005 0.0003 0.0004 – – – – – –
Punctate Nuclear 0.0028 0.0002 0.0002 0.0052 0.0002 0.001 0.0009 0.0002 0.0007 0.0002 0.0004 0.0002 – – – – – –
Vacuole 0.4708 0.5879 0.5252 0.5727 0.506 0.4983 0.4399 0.5305 0.4265 0.4786 0.3914 0.4089 – – – – – –
Vacuole Periphery 0.1905 0.1747 0.1969 0.1775 0.2588 0.2442 0.2093 0.2429 0.207 0.1802 0.3081 0.2891 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.3339 18.8265 9.9786 8.1089 8.7768 20.2968 19.8853 13.0771 15.6881 18.0346
Translational Efficiency 0.5224 0.398 0.5743 0.8315 1.2283 0.9191 0.6416 0.8488 0.7656 0.7148

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene; YBR241C has a paralog, VPS73, that arose from the whole genome duplication
Localization
Cell Percentages vacuolar membrane (60%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Vvs1

Vvs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vvs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available