ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene; YBR242W has a paralog, YGL101W, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 2 |
Bud | 0 | 1 | 0 | 0 | 0 | 4 | 0 | – | 3 | 3 | 3 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 1 | 0 | 0 | 0 | 1 | – | 2 | 3 | 0 | 2 | 1 | 2 | 0 | 6 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 184 | 283 | 180 | 90 | 166 | 161 | 245 | – | 193 | 213 | 96 | 132 | 242 | 408 | 340 | 153 | 137 | 115 | 223 | 270 | 180 | 205 | 268 | 276 |
Endoplasmic Reticulum | 2 | 4 | 1 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 10 | 7 | 7 | 1 | 1 | 0 | 3 | 1 | 1 |
Endosome | 1 | 1 | 0 | 0 | 0 | 0 | 3 | – | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 9 | 2 | 6 | 3 | 0 | 0 | 2 | 3 | 4 |
Golgi | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 3 | 0 | 1 | 2 | 0 | 1 |
Mitochondria | 5 | 1 | 0 | 0 | 2 | 39 | 34 | – | 93 | 88 | 96 | 95 | 1 | 2 | 0 | 6 | 2 | 7 | 2 | 3 | 2 | 4 | 7 | 4 |
Nucleus | 1 | 1 | 0 | 1 | 1 | 1 | 2 | – | 3 | 4 | 3 | 5 | 2 | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 7 | 2 | 2 | 0 | 2 | 0 | 0 | 0 | 4 |
Vac/Vac Membrane | 4 | 0 | 0 | 1 | 5 | 1 | 11 | – | 9 | 6 | 3 | 2 | 0 | 1 | 2 | 18 | 16 | 8 | 3 | 3 | 0 | 2 | 2 | 4 |
Unique Cell Count | 197 | 288 | 182 | 92 | 170 | 180 | 264 | 251 | 275 | 161 | 199 | 244 | 412 | 342 | 180 | 150 | 135 | 247 | 288 | 189 | 230 | 297 | 315 | |
Labelled Cell Count | 204 | 291 | 183 | 93 | 174 | 207 | 300 | 309 | 323 | 203 | 245 | 248 | 415 | 343 | 213 | 175 | 153 | 247 | 288 | 189 | 230 | 297 | 315 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.1 | 5.9 | 6.2 | 5.7 | 3.8 | 4.5 | – | 4.1 | 4.4 | 4.0 | 4.1 | 6.4 | 5.2 | 5.7 | 7.7 | 8.1 | 7.2 | 5.7 | 6.0 | 6.2 |
Std Deviation (1e-4) | 0.9 | 0.8 | 1.1 | 2.4 | 1.4 | 1.2 | 1.2 | – | 1.4 | 1.5 | 1.3 | 1.3 | 1.4 | 1.2 | 1.3 | 1.9 | 1.6 | 1.5 | 1.6 | 1.7 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.06 | -0.05 | -0.63 | -0.39 | – | -0.53 | -0.43 | -0.56 | -0.51 | 0.11 | -0.17 | -0.04 | 0.39 | 0.46 | 0.3 | -0.04 | 0.03 | 0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | -0.9 | -3.8 | -3.0 | – | -6.5 | -6.5 | -9.2 | -8.2 | 0.3 | 0.1 | 0.6 | -4.9 | -3.3 | -4.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 4.5 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.674 | 0.3208 | -0.0503 | -0.4088 | -0.4735 | 0.0207 | 1.5573 | 1.7663 | 1.8547 | 1.3299 | 1.645 | 1.4612 | 3.3201 | 5.0117 | 4.1772 | 4.0448 | 4.2854 | 4.4816 |
Actin | 0.0052 | 0.0021 | 0.0016 | 0.0065 | 0.0078 | 0.0005 | 0.02 | 0.0003 | 0.0046 | 0.0001 | 0.0735 | 0.0001 | 0.0004 | 0.0048 | 0.0003 | 0.0008 | 0.0001 | 0.0013 |
Bud | 0.0007 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0133 | 0.0022 | 0.0124 | 0 | 0.0354 | 0 | 0 | 0.0042 | 0.0001 | 0.0003 | 0.0001 | 0.0006 |
Bud Neck | 0.002 | 0.0003 | 0.0001 | 0.0005 | 0.0004 | 0.0008 | 0.0068 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0012 | 0.0001 | 0.0002 | 0.0001 | 0.0006 |
Bud Periphery | 0.0011 | 0.0007 | 0.0001 | 0.0013 | 0.0002 | 0 | 0.0053 | 0.0006 | 0.0026 | 0 | 0.0387 | 0 | 0 | 0.0023 | 0.0001 | 0.0002 | 0 | 0.0008 |
Bud Site | 0.0056 | 0.0024 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0075 | 0.0033 | 0.0044 | 0 | 0.0149 | 0 | 0.0005 | 0.0216 | 0.0001 | 0.0003 | 0 | 0.0001 |
Cell Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0007 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.3694 | 0.572 | 0.56 | 0.6406 | 0.567 | 0.5381 | 0.3506 | 0.6998 | 0.6624 | 0.5895 | 0.464 | 0.7066 | 0.4729 | 0.5821 | 0.5395 | 0.5509 | 0.7874 | 0.6421 |
Cytoplasmic Foci | 0.0139 | 0.0093 | 0.0104 | 0.0118 | 0.0072 | 0.0125 | 0.0497 | 0.0045 | 0.0085 | 0.0028 | 0.0341 | 0.0028 | 0.0068 | 0.0131 | 0.0038 | 0.0159 | 0.0054 | 0.0069 |
Eisosomes | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0.001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.003 | 0.0023 | 0.0029 | 0.0039 | 0.0038 | 0.0021 | 0.0184 | 0.0022 | 0.0021 | 0.002 | 0.0021 | 0.0009 | 0.003 | 0.002 | 0.0015 | 0.0023 | 0.0013 | 0.0012 |
Endosome | 0.0065 | 0.005 | 0.0056 | 0.0062 | 0.016 | 0.0038 | 0.0448 | 0.001 | 0.0158 | 0.0022 | 0.0104 | 0.0005 | 0.0026 | 0.0017 | 0.002 | 0.0076 | 0.0015 | 0.0015 |
Golgi | 0.001 | 0.0003 | 0.0004 | 0.0004 | 0.0034 | 0.0002 | 0.013 | 0.0001 | 0.0046 | 0.0001 | 0.008 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0001 |
Lipid Particles | 0.0011 | 0.0004 | 0.0003 | 0.0005 | 0.0029 | 0.0002 | 0.0477 | 0.0002 | 0.0006 | 0 | 0.0541 | 0 | 0.0007 | 0.0004 | 0.0001 | 0.0018 | 0.0001 | 0.0001 |
Mitochondria | 0.0066 | 0.0006 | 0.0004 | 0.0005 | 0.0007 | 0.0002 | 0.0122 | 0.0001 | 0.0047 | 0.0001 | 0.0889 | 0 | 0.0002 | 0.001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 |
None | 0.5679 | 0.3827 | 0.4061 | 0.3179 | 0.3747 | 0.4358 | 0.3117 | 0.2829 | 0.2675 | 0.4 | 0.1019 | 0.2874 | 0.508 | 0.3558 | 0.4485 | 0.4049 | 0.2019 | 0.3412 |
Nuclear Periphery | 0.003 | 0.0015 | 0.0026 | 0.0019 | 0.0073 | 0.0006 | 0.042 | 0.0004 | 0.0009 | 0.001 | 0.0019 | 0.0002 | 0.0008 | 0.0007 | 0.0005 | 0.0017 | 0.0003 | 0.0004 |
Nucleolus | 0.0004 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0053 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0 | 0.0001 |
Nucleus | 0.0053 | 0.0053 | 0.0029 | 0.0024 | 0.0038 | 0.0016 | 0.0085 | 0.0009 | 0.0017 | 0.0013 | 0.0017 | 0.0007 | 0.0011 | 0.0026 | 0.0017 | 0.006 | 0.0011 | 0.0013 |
Peroxisomes | 0.0026 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0074 | 0.0001 | 0.0022 | 0 | 0.0586 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0008 | 0 | 0.0002 |
Punctate Nuclear | 0.0026 | 0.003 | 0.0037 | 0.0014 | 0.0021 | 0.0024 | 0.0214 | 0.0004 | 0.0005 | 0.0003 | 0.0039 | 0.0002 | 0.0011 | 0.0029 | 0.0004 | 0.0041 | 0.0002 | 0.0007 |
Vacuole | 0.0014 | 0.0089 | 0.0014 | 0.0019 | 0.0013 | 0.0007 | 0.0061 | 0.0007 | 0.0036 | 0.0004 | 0.0036 | 0.0003 | 0.001 | 0.0011 | 0.0007 | 0.0009 | 0.0004 | 0.0004 |
Vacuole Periphery | 0.0002 | 0.0008 | 0.0003 | 0.0004 | 0.0005 | 0.0001 | 0.0074 | 0.0001 | 0.0004 | 0.0001 | 0.0013 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.934 | 34.3925 | 26.5415 | 20.4622 | 21.948 | 12.3045 | 26.0806 | 22.062 | 18.867 | 25.854 |
Translational Efficiency | 1.0139 | 1.6369 | 1.5687 | 1.7534 | 1.799 | 2.203 | 1.3923 | 1.5083 | 1.4213 | 1.1984 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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