Standard name
Human Ortholog
Description Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0 0.05 0.07 0.07 0.07 0 0.07 0.09 0 0.05 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.93 0.89 0.94 0.89 0.73 0.72 0.76 0.65 0.66 0.69 0.99 0.99 0.99 0.98 0.96 0.97 0.95 0.95 0.89 0.94 0.92 0.93
Endoplasmic Reticulum 0 0 0.07 0 0 0 0 0.08 0 0.06 0.05 0 0 0 0 0.05 0.05 0 0 0 0.07 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.18 0.3 0.2 0.22 0.43 0.4 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 3 2 9 19 15 4 8 3 4 0 0 1 0 0 0 0 1 0 0 0 0
Bud 0 0 1 0 0 4 9 3 1 6 6 2 0 1 0 1 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 4 7 17 10 27 45 42 29 20 25 44 4 16 9 10 3 8 0 0 0 0 0 0
Cytoplasm 220 295 321 238 284 481 486 455 301 310 251 331 179 342 314 281 302 267 208 285 315 221 230 291
Endoplasmic Reticulum 4 6 24 8 7 12 20 52 14 29 18 14 4 2 2 13 16 8 5 4 23 2 6 10
Endosome 0 0 0 0 1 0 2 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 6 0 1 1 4 1 0 0 0 0 0 0 0 0 1 0 0 3 0 0 0
Mitochondria 1 0 2 4 11 95 201 125 86 207 150 144 0 0 0 2 2 2 0 0 2 0 0 0
Nucleus 1 0 1 1 0 1 2 3 2 2 1 4 0 1 1 3 2 0 0 1 1 4 0 0
Nuclear Periphery 0 0 0 0 0 1 4 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 19 6 3 13 27 24 7 13 20 0 4 1 3 10 6 0 3 4 4 5 4
Unique Cell Count 226 300 345 268 302 542 665 631 398 480 379 479 181 347 316 288 313 276 219 301 355 235 251 313
Labelled Cell Count 229 305 358 296 321 634 804 727 468 589 468 565 187 366 328 313 335 292 219 301 355 235 251 313


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.2 18.9 14.5 13.9 11.3 9.2 8.7 9.0 9.2 7.6 7.5 8.1 18.3 17.0 15.7 21.8 19.4 21.4 18.8 18.8 20.3
Std Deviation (1e-4) 2.7 3.6 2.7 4.3 2.1 2.0 3.1 2.6 5.6 1.8 1.9 3.9 3.8 3.5 3.3 5.6 4.2 4.6 4.1 4.4 4.2
Intensity Change (Log2) – – – -0.07 -0.37 -0.66 -0.75 -0.69 -0.66 -0.93 -0.96 -0.84 0.33 0.22 0.11 0.58 0.42 0.56 0.37 0.37 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 2.8 2.5 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.7 1.0 2.2 3.2 3.2 3.3 1.7 3.0 4.2 0 1.9 0.7 1.1 0 0.7
Cytoplasm -1.8 0.5 -2.1 -7.5 -7.7 -6.4 -9.5 -8.8 -8.3 2.9 3.6 4.2 2.6 2.0 2.0
Endoplasmic Reticulum -2.2 -2.8 -3.5 -2.9 0.7 -2.1 -0.5 -1.3 -2.7 -2.3 -4.4 -4.2 -1.3 -1.0 -2.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.8 7.9 11.1 8.5 8.8 13.9 12.9 10.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 4.7 0 0 0 3.6 4.3 0 3.1 3.5 0 0 0 0 2.9 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.5744 14.5445 12.9989 10.7998 10.9946 12.9752 10.693 15.627 14.5393 13.1512 12.0621 13.9782 11.6088 14.9835 13.2966 14.4813 12.1968 13.9206
Actin 0.0185 0.0021 0.0108 0.0088 0.0276 0.0018 0.038 0.0012 0.0016 0.0083 0.0126 0.0116 0.024 0.0127 0.0581 0.0001 0.0079 0.0008
Bud 0.0011 0.0008 0.0017 0.0009 0.0011 0.0008 0.0011 0.0004 0.0005 0.0035 0.003 0.0008 0.001 0.0005 0.0009 0.0013 0.0048 0.0006
Bud Neck 0.0015 0.0005 0.0012 0.001 0.0011 0.0015 0.0008 0.0002 0.0004 0.0007 0.0011 0.001 0.0011 0.0003 0.0028 0.0004 0.0018 0.0012
Bud Periphery 0.0012 0.0003 0.0019 0.0008 0.0017 0.0025 0.0012 0.0001 0.0002 0.003 0.0095 0.0006 0.0012 0.0002 0.0006 0.0004 0.0046 0.0003
Bud Site 0.0027 0.012 0.0015 0.0009 0.0016 0.0003 0.0026 0.0006 0.001 0.0049 0.0065 0.0003 0.0071 0.0018 0.006 0.0002 0.0061 0.0001
Cell Periphery 0.0029 0.0011 0.0014 0.0005 0.0006 0.0015 0.0011 0.0002 0.0001 0.0012 0.0041 0.0001 0.0012 0.0004 0.0003 0.0002 0.001 0.0001
Cytoplasm 0.6933 0.9212 0.7876 0.4765 0.6296 0.8247 0.6676 0.9293 0.8758 0.6788 0.6904 0.7388 0.7061 0.9611 0.8258 0.713 0.6072 0.8211
Cytoplasmic Foci 0.0171 0.0091 0.0148 0.0341 0.0294 0.0189 0.018 0.0107 0.0153 0.0265 0.0316 0.0232 0.0215 0.0038 0.0154 0.0162 0.0565 0.0156
Eisosomes 0.0003 0 0.0001 0.0001 0.0003 0.0001 0.0003 0 0 0 0.0006 0.0001 0.0004 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0911 0.0169 0.0327 0.0232 0.0191 0.0323 0.0772 0.0164 0.0126 0.0097 0.0121 0.088 0.0583 0.0052 0.0174 0.019 0.0078 0.0446
Endosome 0.0641 0.0193 0.0571 0.1703 0.142 0.0733 0.0836 0.0158 0.0478 0.1747 0.1523 0.0892 0.0545 0.0056 0.0455 0.1087 0.1124 0.0696
Golgi 0.0112 0.0038 0.0125 0.0307 0.015 0.0103 0.0172 0.0045 0.0034 0.0268 0.0323 0.0167 0.0103 0.001 0.0107 0.0033 0.0559 0.0043
Lipid Particles 0.0085 0.0008 0.0036 0.0322 0.0378 0.0008 0.0036 0.0021 0.0007 0.0011 0.0012 0.0015 0.009 0.0001 0.0012 0.0001 0.0014 0
Mitochondria 0.0059 0.0009 0.012 0.0875 0.0149 0.0092 0.0086 0.0021 0.0148 0.0112 0.0192 0.009 0.0164 0.0007 0.0016 0.0015 0.0987 0.0024
None 0.0085 0.0005 0.0014 0.0009 0.0089 0.0015 0.0046 0.0004 0.0011 0.0005 0.0024 0.0012 0.0152 0.0003 0.0006 0.0005 0.0008 0.0005
Nuclear Periphery 0.0174 0.0008 0.0047 0.0055 0.0096 0.0038 0.0158 0.0012 0.0019 0.0019 0.0021 0.004 0.0095 0.0004 0.0023 0.004 0.0008 0.002
Nucleolus 0.001 0 0.0001 0.0001 0.0014 0.0001 0.0002 0 0.0001 0.0001 0.0001 0 0.0007 0 0 0 0.0001 0
Nucleus 0.0052 0.0007 0.0019 0.0017 0.0056 0.0014 0.0097 0.0011 0.0013 0.0012 0.001 0.0022 0.0031 0.0007 0.0011 0.0015 0.0011 0.0009
Peroxisomes 0.0029 0.0002 0.006 0.0068 0.0093 0.0002 0.0019 0.002 0.0003 0.0004 0.0003 0.0007 0.0031 0.0001 0.0005 0.0001 0.0048 0.0001
Punctate Nuclear 0.0045 0.0001 0.0004 0.0009 0.0087 0.0004 0.0018 0.0004 0.0004 0.0002 0.0002 0.0004 0.0029 0 0.0006 0.0001 0.0005 0.0001
Vacuole 0.0358 0.0076 0.0412 0.092 0.0261 0.0096 0.0367 0.0099 0.0153 0.0349 0.0119 0.0066 0.046 0.0045 0.0065 0.102 0.0167 0.0219
Vacuole Periphery 0.0054 0.0012 0.0054 0.0246 0.0087 0.0049 0.0084 0.0015 0.0052 0.0104 0.0054 0.0038 0.0073 0.0006 0.0019 0.0276 0.0091 0.0137

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.0104 61.7528 50.7771 32.834 48.4133 39.1856 33.5394 27.9046 24.8216 36.0809
Translational Efficiency 2.253 1.9434 1.9597 2.2908 1.9718 2.096 1.758 1.9144 1.6267 1.7328

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Rei1

Rei1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rei1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available