ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.09 0.06 0 0 0 0 0 0 0 0 0 0.12 0.07 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.05 0.05 0 0 0 0 0 0.11 0.11 0.13 0 0.08 0.13 0 0 0 0 0 0
Cytoplasm 0.25 0.19 0.17 0.13 0.1 0.2 0.36 0.4 0.48 0.47 0.47 0.17 0.17 0.37 0.63 0.51 0.46 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.67 0.73 0.63 0.7 0.79 0.55 0.23 0.28 0.13 0.06 0.09 0.8 0.8 0.57 0 0 0 0.78 0.84 0.88 0.86 0.78 0.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.09 0.09 0 0.14 0.15 0.23 0.43 0.5 0.43 0 0 0 0.12 0.06 0.13 0.06 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0.16 0.13 0.17 0.17 0.34 0.15 0.12 0.1 0.12 0 0 0 0.12 0.24 0.19 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 15 28 32 16 8 7 4 2 4 4 4 10 6 12 7 1 1 10 3 13 19
Bud 0 1 6 1 1 6 6 15 13 22 7 0 0 1 2 4 1 1 0 2 4 9 10
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 0 1 2 2
Bud Site 0 0 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0
Cell Periphery 1 0 18 11 29 25 16 7 8 7 9 29 29 34 0 13 10 0 0 4 1 8 9
Cytoplasm 13 19 71 40 53 98 225 252 263 205 242 44 44 96 32 83 36 2 5 6 3 15 15
Endoplasmic Reticulum 35 72 258 212 431 271 145 179 72 28 47 208 208 149 2 7 2 92 101 420 209 332 424
Endosome 0 0 6 10 4 5 13 6 2 1 3 0 0 0 1 6 2 0 0 1 1 3 2
Golgi 0 0 1 8 5 1 0 0 0 0 0 0 0 0 0 1 2 0 0 1 0 3 4
Mitochondria 1 5 35 26 14 71 96 145 235 218 220 0 0 0 6 10 10 6 3 16 7 15 16
Nucleus 0 1 0 1 0 0 5 11 2 3 6 2 2 3 1 6 0 0 0 0 0 0 1
Nuclear Periphery 2 0 4 6 7 14 10 12 7 4 12 0 0 0 0 1 2 4 0 2 1 3 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 1 0 1 0 0 1 1 3 1 0 0 0 0 1 1
Vac/Vac Membrane 2 7 67 40 91 84 212 93 66 43 62 9 8 5 6 39 15 2 4 7 6 18 20
Unique Cell Count 52 98 408 303 546 491 623 631 551 439 517 259 260 263 51 163 78 118 121 479 244 429 531
Labelled Cell Count 54 105 482 383 667 591 736 729 675 534 615 296 295 299 58 187 89 118 121 479 244 429 531


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 8.9 7.1 9.2 10.3 7.6 6.1 5.3 4.2 4.3 4.3 9.3 9.3 8.2 6.4 8.3 9.3 11.1 11.8 12.6
Std Deviation (1e-4) 1.7 2.1 1.4 1.9 1.6 1.4 1.3 1.0 0.7 0.8 0.7 1.7 1.7 1.3 2.0 2.7 2.5 2.6 3.4 3.2
Intensity Change (Log2) 0.37 0.53 0.09 -0.21 -0.43 -0.74 -0.71 -0.72 0.39 0.39 0.21 -0.15 0.23 0.39 0.64 0.73 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.1 1.5 -0.3 -2.5 -2.8 0 0 0 -1.6 -1.6 0.1 0 1.9 0
Bud 0 0 -0.3 0 1.0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 0.6 0.5 -1.6 -3.4 0 0 0 3.3 3.3 4.0 0 1.7 0
Cytoplasm -1.5 -3.5 1.0 6.5 7.7 9.7 9.1 9.4 -0.1 -0.2 5.6 7.3 8.2 5.6
Endoplasmic Reticulum 1.9 5.4 -2.4 -12.9 -11.1 0 0 0 4.7 4.6 -1.7 -8.1 -12.8 -9.8
Endosome 1.6 0 0 0.7 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -4.2 2.7 3.2 6.0 0 0 0 -4.8 -4.9 -4.9 0 -1.0 1.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.0 0.8 1.2 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.2 0.1 0.3 6.2 -0.7 -2.0 -2.9 -1.9 -5.1 -5.3 -5.9 -0.9 2.1 0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4896 3.2391 2.8764 2.6998 2.267 3.306 1.6576 3.9921 2.3916 2.6108 2.0898 2.6017 1.91 4.7819 2.9191 3.6542 2.1402 3.006
Actin 0.0425 0.002 0.014 0.0394 0.0009 0.002 0.0071 0.0006 0.0074 0.0031 0.0023 0.0045 0.0092 0.0007 0.0016 0.0024 0.0005 0.0015
Bud 0.0003 0.002 0.0014 0.0005 0.0005 0 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0001 0 0.0001 0
Bud Neck 0.0029 0.0004 0.0007 0.0372 0.0035 0.002 0.0007 0.0005 0.0004 0.0011 0.0005 0.0007 0.0012 0.0002 0.0003 0.0004 0.0003 0.0026
Bud Periphery 0.0005 0.0008 0.001 0.0007 0.0008 0.0001 0.0004 0.0001 0.0002 0.0005 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Site 0.0032 0.0052 0.003 0.0176 0.0177 0.0001 0.0057 0.0053 0.0013 0.0004 0.0005 0.0001 0.0032 0.0023 0.0011 0.0001 0.0002 0.0001
Cell Periphery 0.0012 0.0094 0.0149 0.0008 0.0012 0.0005 0.0013 0.0032 0.0017 0.0011 0.0007 0.0008 0.0013 0.0015 0.0011 0.0004 0.0006 0.0021
Cytoplasm 0.2388 0.3751 0.247 0.2229 0.2614 0.4068 0.1568 0.2049 0.1884 0.1594 0.1537 0.1791 0.1492 0.1463 0.1746 0.1569 0.2078 0.1794
Cytoplasmic Foci 0.0338 0.0171 0.0166 0.0074 0.0082 0.0128 0.0259 0.0135 0.0128 0.0074 0.0161 0.0084 0.0139 0.0056 0.0063 0.0039 0.0044 0.0094
Eisosomes 0.0009 0.0002 0.0002 0.0007 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0002 0.0003 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.4345 0.436 0.4681 0.4089 0.4419 0.4256 0.5829 0.6443 0.6385 0.6404 0.6682 0.6545 0.6622 0.757 0.7026 0.7167 0.6325 0.7241
Endosome 0.0353 0.0134 0.0295 0.0235 0.0137 0.0139 0.0388 0.0228 0.0193 0.04 0.0314 0.0206 0.0298 0.0097 0.0122 0.0353 0.0053 0.0161
Golgi 0.0079 0.001 0.0101 0.0132 0.0011 0.0011 0.0101 0.0011 0.0052 0.0025 0.0024 0.0024 0.0052 0.0008 0.0021 0.0031 0.0003 0.0005
Lipid Particles 0.0144 0.0053 0.0109 0.0117 0.0044 0.0064 0.0295 0.0143 0.0166 0.0036 0.0073 0.0072 0.0182 0.0131 0.01 0.0147 0.0024 0.0035
Mitochondria 0.0018 0.0006 0.0021 0.0018 0.0006 0.0006 0.0018 0.0003 0.0037 0.0041 0.0023 0.0039 0.0016 0.0001 0.0004 0.0004 0.0002 0.0001
None 0.1232 0.0925 0.1133 0.1563 0.2042 0.1007 0.0963 0.063 0.0659 0.1175 0.0504 0.0943 0.062 0.0392 0.0688 0.0038 0.1263 0.0349
Nuclear Periphery 0.0209 0.0174 0.0245 0.0398 0.0129 0.016 0.0178 0.0127 0.0199 0.0086 0.0281 0.0136 0.0274 0.0134 0.0094 0.0475 0.0063 0.0123
Nucleolus 0.0005 0.0004 0.0011 0.0005 0.0033 0.0001 0.0003 0.0003 0.0001 0.0001 0.0006 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0
Nucleus 0.0093 0.0064 0.0157 0.0073 0.0102 0.0058 0.0021 0.0028 0.0022 0.0022 0.0237 0.002 0.0033 0.0023 0.0014 0.0041 0.0088 0.0025
Peroxisomes 0.0098 0.0017 0.0082 0.0015 0.0004 0.0003 0.0094 0.0001 0.0072 0.0001 0.0003 0.0003 0.0026 0.0001 0.0022 0.0001 0 0.0001
Punctate Nuclear 0.0075 0.0038 0.0054 0.0023 0.003 0.0011 0.0046 0.0018 0.0009 0.001 0.003 0.0012 0.002 0.001 0.0007 0.0016 0.0007 0.0007
Vacuole 0.0096 0.0083 0.0102 0.0045 0.0093 0.0037 0.0076 0.0076 0.007 0.0055 0.0065 0.0049 0.006 0.0062 0.0044 0.0066 0.003 0.0094
Vacuole Periphery 0.0013 0.0007 0.0022 0.0015 0.0005 0.0004 0.0008 0.0005 0.0008 0.001 0.0015 0.0009 0.0009 0.0004 0.0004 0.0016 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.0215 45.3241 66.1248 70.2951 44.5674 81.593 56.5073 72.6075 85.9261 63.9622
Translational Efficiency 0.6353 0.6668 0.6359 0.8273 0.7341 0.6793 0.7147 0.6175 0.7163 0.6039

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
85 601 430 1032 1545 1264 655 1503 1630 1865 1085 2535

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1165.68 1057.04 1262.03 1688.89 929.00 887.98 1496.90 1534.95 941.34 942.46 1403.82 1597.62
Standard Deviation 163.47 184.72 172.75 341.92 146.68 126.54 241.76 301.89 156.70 167.60 245.58 327.64
Intensity Change Log 2 -0.141142 0.114574 0.534904 -0.065151 0.688227 0.724441 -0.106945 0.397379 0.622050

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007321 0.001039 0.001530 0.001623 0.000513 0.001121 0.001151 0.002474 0.000868 0.001095 0.001301 0.002127
Bud Neck 0.010437 0.001456 0.002548 0.002123 0.000454 0.001207 0.001299 0.003055 0.000975 0.001288 0.001794 0.002675
Bud Site 0.028931 0.006424 0.007672 0.012447 0.003468 0.003836 0.004810 0.017093 0.004796 0.004670 0.005944 0.015202
Cell Periphery 0.003060 0.002522 0.000841 0.026874 0.002866 0.000796 0.003100 0.018791 0.002877 0.001352 0.002205 0.022082
Cytoplasm 0.087352 0.075967 0.038333 0.012844 0.054414 0.118570* 0.005619 0.024446 0.056132 0.104841* 0.018584 0.019723
Cytoplasmic Foci 0.021517 0.014930 0.006994 0.001925 0.008928 0.036164 0.000392 0.003684 0.009584 0.029321 0.003009 0.002968
Eisosomes 0.001426 0.000424 0.000049 0.000294 0.000336 0.000402 0.000079 0.000189 0.000393 0.000409 0.000067 0.000231
Endoplasmic Reticulum 0.576518* 0.833300* 0.785700* 0.875303* 0.886076* 0.714587* 0.962257* 0.848642* 0.869933* 0.752842* 0.892285* 0.859496*
Endosome 0.014579 0.005890 0.013182 0.007822 0.003343 0.009255 0.004834 0.012059 0.003929 0.008171 0.008143 0.010334
Golgi 0.001563 0.006127 0.000922 0.005765 0.006354 0.013567 0.001167 0.006193 0.006104 0.011169 0.001070 0.006019
Lipid Particles 0.034607 0.014475 0.009852 0.027755 0.010991 0.042303 0.004848 0.031337 0.012223 0.033335 0.006831 0.029879
Mitochondria 0.000198 0.001793 0.000180 0.000642 0.004646 0.007797 0.000110 0.000740 0.004414 0.005862 0.000138 0.000700
Mitotic Spindle 0.001276 0.000469 0.001413 0.001247 0.000355 0.000354 0.000082 0.001440 0.000403 0.000391 0.000609 0.001361
None 0.000076 0.003432 0.000134 0.000423 0.005041 0.003164 0.000287 0.001443 0.004782 0.003251 0.000227 0.001027
Nuclear Periphery 0.006249 0.001569 0.012549 0.001140 0.000576 0.004492 0.000487 0.000738 0.000872 0.003550 0.005267 0.000902
Nuclear Periphery Foci 0.033013 0.015116 0.082192 0.006364 0.003224 0.009509 0.005870 0.011362 0.004777 0.011315 0.036117 0.009328
Nucleolus 0.000199 0.000095 0.000039 0.000013 0.000208 0.000235 0.000005 0.000015 0.000208 0.000190 0.000018 0.000014
Nucleus 0.030952 0.005247 0.007196 0.000987 0.002488 0.013133 0.000173 0.000792 0.003973 0.010592 0.002956 0.000871
Peroxisomes 0.001177 0.000916 0.000240 0.000148 0.002688 0.002556 0.000051 0.000677 0.002610 0.002027 0.000126 0.000462
Vacuole 0.139124 0.008249 0.028090 0.012593 0.002233 0.014613 0.003176 0.013508 0.009372 0.012562 0.013050 0.013136
Vacuole Periphery 0.000423 0.000563 0.000343 0.001667 0.000796 0.002337 0.000203 0.001321 0.000777 0.001765 0.000258 0.001462

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.85 1.61 1.64 -1.33 0.06 -1.43 -0.87 -4.84 -2.44 -1.67 -0.64 -0.97 -3.76 -2.66 -1.37
Bud Neck 2.39 2.04 2.16 -1.43 0.53 -3.24 -1.83 -6.84 -4.45 -3.16 -1.01 -2.09 -5.37 -4.45 -2.14
Bud Site 2.27 2.11 1.62 -2.42 -1.70 -0.37 -0.82 -6.63 -6.17 -4.95 0.22 -0.74 -6.50 -6.49 -4.88
Cell Periphery 0.30 1.98 -9.58 -10.04 -11.35 6.59 -0.43 -9.59 -11.01 -9.24 3.80 1.59 -13.98 -15.11 -14.45
Cytoplasm 0.82 2.83 4.30 9.86 4.75 -11.50 17.02 8.39 17.46 -8.24 -10.29 10.93 11.34 20.29 -0.68
Cytoplasmic Foci 1.51 2.87 3.87 5.29 3.88 -10.48 8.26 4.42 13.21 -6.20 -9.44 5.64 6.16 14.21 0.22
Eisosomes 1.14 1.61 1.31 0.94 -3.31 -0.78 4.35 2.43 3.03 -3.32 -0.23 4.69 2.09 2.65 -4.57
Endoplasmic Reticulum -6.52 -5.25 -7.41 -2.69 -5.98 15.81 -10.99 5.02 -10.97 14.52 12.18 -2.40 1.84 -11.16 4.25
Endosome 3.06 0.56 2.44 -1.79 3.84 -6.82 -2.52 -12.16 -2.94 -8.50 -5.93 -6.33 -11.44 -3.03 -2.92
Golgi -5.18 1.25 -3.57 0.26 -4.24 -4.79 6.70 0.09 4.51 -5.19 -4.38 7.16 0.05 4.33 -6.69
Lipid Particles 1.80 2.17 0.56 -3.80 -5.29 -11.56 4.09 -7.66 2.79 -9.81 -9.78 3.52 -8.14 1.04 -10.97
Mitochondria -2.63 0.50 -2.35 1.80 -2.54 -1.77 4.71 3.99 4.70 -3.91 -1.05 4.68 4.01 4.96 -4.65
Mitotic Spindle 1.26 -0.12 0.06 -1.06 0.17 -0.01 1.28 -1.94 -2.00 -2.46 0.08 -0.77 -2.09 -2.21 -1.48
None -2.44 -0.85 -3.19 2.18 -2.73 1.60 5.31 3.50 1.77 -1.96 1.42 5.46 4.23 2.87 -2.28
Nuclear Periphery 2.01 -2.60 2.20 1.06 8.45 -5.84 0.57 -1.49 5.59 -2.40 -5.52 -7.54 -0.55 5.59 7.63
Nuclear Periphery Foci 2.41 -4.84 3.50 3.37 11.50 -6.38 -1.60 -7.23 -1.70 -3.04 -6.02 -10.41 -5.28 1.02 8.55
Nucleolus 1.44 1.98 2.29 3.02 2.45 -0.14 1.33 1.25 3.75 -5.04 0.15 1.31 1.34 4.32 0.98
Nucleus 2.84 2.63 3.19 5.72 4.11 -9.10 7.30 4.63 10.76 -4.16 -5.79 1.38 4.68 11.84 3.32
Peroxisomes 0.95 3.20 3.71 3.16 1.44 0.28 7.89 4.53 4.56 -2.11 1.52 7.77 5.95 5.71 -1.80
Vacuole 5.93 5.03 5.70 -2.76 4.45 -7.71 -1.64 -9.80 0.03 -8.38 -1.00 -1.64 -2.04 -1.20 -0.15
Vacuole Periphery -0.85 0.57 -3.25 -2.77 -3.50 -3.23 4.07 -2.31 2.03 -5.88 -2.91 3.78 -3.05 0.76 -6.29
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

YBR287W

YBR287W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybr287w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available