Standard name
Human Ortholog
Description Regulatory subunit of acetolactate synthase; acetolactate synthase catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 – – 0 0 0 0 0 0 0 0 0 0.05 0 0 – – – 0 0 0.07 0.09 0.11 0.2
Bud 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 – – 0 0 0 0 0 0 0 0 0 0 0 0.06 – – – 0 0 0 0 0 0
Cytoplasm 0 – – 0 0 0 0 0 0 0 0 0 0.13 0 0.07 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.07 0 0 0 0
Endosome 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 – – 0 0.1 0 0 0 0 0 0 0 0.13 0.22 0.23 – – – 0.13 0.18 0.31 0.13 0.22 0.17
Mitochondria 0.84 – – 0.78 0.83 0.82 0.81 0.81 0.89 0.75 0.88 0.79 0.79 0.75 0.66 – – – 0.69 0.43 0.59 0.64 0.52 0.4
Nucleus 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.42 – – 0.22 0.24 0.22 0.21 0.22 0.25 0.4 0.35 0.26 0.16 0.11 0.08 – – – 0.07 0.11 0 0 0 0
Peroxisomes 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.05 0.06 0.05
SpindlePole 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.05 – – 0 0.07 0.06 0.11 0.06 0 0 0.06 0.1 0.05 0.06 0.12 – – – 0 0.16 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 – – 1 2 0 4 1 0 1 0 0 2 2 2 – – – 1 0 1 6 14 30
Bud 0 – – 0 0 0 0 1 0 2 0 1 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 1 0 0
Bud Site 0 – – 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 – – 0 1 0 0 0 0 0 0 1 0 2 6 – – – 0 0 0 0 0 0
Cytoplasm 0 – – 1 1 1 7 4 2 0 0 0 5 4 7 – – – 0 1 0 0 2 2
Endoplasmic Reticulum 0 – – 0 0 0 1 0 0 0 0 0 0 0 0 – – – 0 1 0 0 0 1
Endosome 0 – – 0 0 0 0 2 0 0 0 0 0 0 0 – – – 0 0 0 0 1 3
Golgi 0 – – 1 7 1 4 6 0 1 0 0 5 21 23 – – – 5 5 4 9 27 26
Mitochondria 16 – – 18 58 41 127 122 67 82 15 73 30 73 67 – – – 28 12 9 44 65 60
Nucleus 0 – – 0 0 0 1 0 1 0 0 0 0 0 0 – – – 0 0 0 0 1 3
Nuclear Periphery 0 – – 0 0 1 1 1 0 0 0 0 0 1 0 – – – 0 0 0 0 0 0
Nucleolus 8 – – 5 17 11 32 33 19 44 6 24 6 11 8 – – – 3 3 0 2 0 3
Peroxisomes 0 – – 1 2 2 0 0 0 0 0 1 0 0 2 – – – 1 0 0 3 7 8
SpindlePole 0 – – 0 0 0 0 2 0 1 0 0 0 0 0 – – – 0 0 0 0 0 1
Vac/Vac Membrane 1 – – 1 5 3 17 9 1 4 1 9 2 6 12 – – – 0 4 0 1 3 7
Unique Cell Count 19 23 70 50 156 151 75 110 17 92 38 97 102 42 30 16 70 126 153
Labelled Cell Count 26 28 93 60 194 182 90 135 22 109 50 120 127 42 30 16 70 126 153


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.0 – – 39.5 42.8 38.9 34.1 31.6 27.0 24.0 22.7 22.7 44.5 41.1 37.7 – – – 40.7 42.1 40.4
Std Deviation (1e-4) 8.7 – – 13.7 12.0 12.1 12.0 10.8 10.3 8.5 8.2 8.5 15.0 14.2 11.9 – – – 13.7 15.2 14.6
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 63.7462 87.4468 78.861 62.1936 65.8861 74.6593 82.2778 98.6295 89.8837 77.3816 83.3565 84.4095 96.9819 103.1183 91.8427 102.224 104.493 87.7233
Actin 0.0626 0.0042 0.0024 0.0008 0.0015 0.0006 0.0074 0.0023 0.0055 0.0002 0.0013 0.0007 0.0081 0.0002 0.0012 0 0 0.0002
Bud 0.0005 0.0004 0 0 0 0 0.0004 0 0.0001 0 0.0001 0 0.0002 0 0 0 0 0
Bud Neck 0.0007 0.0001 0 0 0 0 0.0021 0 0.0006 0 0 0 0.0002 0 0 0 0 0
Bud Periphery 0.0007 0.0002 0 0.0001 0 0 0.0003 0 0.0001 0 0.0004 0 0.0004 0 0 0 0 0
Bud Site 0.0031 0.0015 0 0.0001 0.0001 0 0.0068 0 0.0063 0 0.0002 0 0.0014 0 0 0 0 0
Cell Periphery 0.0005 0.0008 0.0002 0.0003 0.0002 0 0.0003 0.0001 0.0015 0 0.0005 0.0001 0.0002 0.0001 0.0001 0 0 0.0002
Cytoplasm 0.0059 0 0 0.0003 0 0 0.0025 0 0 0 0 0 0 0 0 0 0 0
Cytoplasmic Foci 0.0038 0.0001 0.0002 0.0538 0.0009 0 0.0122 0 0.0001 0 0.0003 0.0001 0.0011 0 0.0001 0 0 0.0001
Eisosomes 0.0024 0.0166 0.0035 0.001 0.0009 0.0008 0.0006 0.0014 0.0106 0.0004 0.0003 0.0003 0.0018 0.0013 0.0034 0.0002 0.0001 0.0012
Endoplasmic Reticulum 0.005 0 0 0 0 0 0.001 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.0117 0.0001 0.0001 0.0003 0.0005 0 0.0145 0.0003 0 0 0.0005 0.0001 0.0008 0 0 0 0 0
Golgi 0.0048 0.0003 0.0005 0.0009 0.0039 0 0.0066 0.0044 0 0 0.0008 0.0017 0.0042 0 0.0001 0 0 0.0002
Lipid Particles 0.0168 0.0068 0.0074 0.0202 0.0395 0.0002 0.0397 0.001 0.0002 0.0007 0.0219 0.011 0.0083 0.0007 0.0008 0.0003 0 0.0122
Mitochondria 0.8105 0.9501 0.9741 0.8718 0.8945 0.9957 0.8888 0.9769 0.9715 0.9968 0.9356 0.9782 0.9553 0.9923 0.9885 0.9979 0.9997 0.9554
None 0.006 0 0 0.0038 0 0 0.0009 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Nuclear Periphery 0.0259 0 0 0.0001 0.0001 0 0.0004 0.0002 0 0 0.0001 0 0.0001 0 0 0 0 0.0005
Nucleolus 0.0031 0.0031 0.0002 0.0309 0.0002 0.0001 0.0007 0.0001 0.0002 0 0.001 0 0.0015 0.0001 0.0001 0 0 0.0173
Nucleus 0.0126 0.0003 0.0001 0.0014 0 0 0.0003 0 0.0001 0 0.0002 0 0.0002 0 0 0 0 0.0012
Peroxisomes 0.0072 0.0019 0.0033 0.0026 0.0485 0.0002 0.0083 0.0004 0.0027 0.0013 0.0044 0.0019 0.0087 0.0005 0.0051 0.0001 0.0001 0.0003
Punctate Nuclear 0.0095 0 0 0.0016 0 0 0.001 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.0018 0.001 0.0006 0.0013 0.0005 0.0001 0.0014 0.0006 0.0003 0 0.0047 0.0002 0.0007 0.0002 0 0.0001 0 0.0003
Vacuole Periphery 0.0051 0.0124 0.0073 0.0087 0.0087 0.0021 0.0034 0.0121 0.0002 0.0005 0.0276 0.0055 0.0066 0.0044 0.0005 0.0014 0.0001 0.0108

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 414.6665 410.4598 365.1798 352.8749 403.8018 495.6642 454.6939 431.38 415.7837 365.5367
Translational Efficiency 0.7541 0.8068 0.7802 0.7946 0.804 0.705 0.7043 0.7943 0.7847 0.8019

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
104 141 2283 1090 353 1113 218 1038 457 1254 2501 2128

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3282.84 4534.25 3829.40 2504.38 3217.20 3372.22 3362.16 2400.67 3232.14 3502.88 3788.67 2453.79
Standard Deviation 833.57 1533.89 884.95 696.96 1104.69 1224.03 717.74 689.68 1049.52 1314.95 881.56 695.35
Intensity Change Log 2 — 0.465920 0.222174 -0.390491 — 0.067893 0.063583 -0.422369 — 0.282585 0.145856 -0.406181

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005830 0.023342 0.003810 0.006916 0.009070 0.015723 0.016602 0.011655 0.008333 0.016580 0.004925 0.009228
Bud Neck 0.002766 0.008582 0.000480 0.002218 0.002709 0.003410 0.010791 0.001359 0.002722 0.003992 0.001379 0.001799
Bud Site 0.001053 0.003651 0.000518 0.002395 0.001600 0.002310 0.006816 0.001520 0.001475 0.002461 0.001067 0.001968
Cell Periphery 0.000049 0.000226 0.000052 0.000261 0.000111 0.000102 0.000183 0.000169 0.000097 0.000116 0.000064 0.000216
Cytoplasm 0.000234 0.000415 0.000088 0.000335 0.000297 0.000429 0.000538 0.000214 0.000282 0.000428 0.000128 0.000276
Cytoplasmic Foci 0.061638 0.062053 0.008387 0.012081 0.040736 0.043172 0.098542 0.008614 0.045493 0.045295 0.016245 0.010390
Eisosomes 0.006836 0.010007 0.002563 0.001334 0.005588 0.005315 0.003411 0.003769 0.005872 0.005842 0.002637 0.002522
Endoplasmic Reticulum 0.000107 0.000198 0.000031 0.000243 0.000105 0.000191 0.000246 0.000212 0.000105 0.000191 0.000050 0.000228
Endosome 0.000666 0.001408 0.000285 0.001706 0.000454 0.000809 0.002507 0.000963 0.000502 0.000876 0.000479 0.001343
Golgi 0.027672 0.035785 0.008908 0.026728 0.012905 0.024596 0.028418 0.017054 0.016266 0.025854 0.010609 0.022009
Lipid Particles 0.008763 0.016948 0.002307 0.005255 0.009446 0.017989 0.020265 0.002571 0.009291 0.017872 0.003872 0.003946
Mitochondria 0.824155* 0.763008* 0.958479* 0.900249* 0.870920* 0.828902* 0.722114* 0.935361* 0.860277* 0.821493* 0.937876* 0.917376*
Mitotic Spindle 0.000558 0.016725 0.000468 0.001298 0.000591 0.003494 0.013888 0.000532 0.000583 0.004982 0.001638 0.000924
None 0.007415 0.006281 0.003713 0.005131 0.007295 0.005388 0.006007 0.003484 0.007323 0.005488 0.003913 0.004328
Nuclear Periphery 0.000501 0.001669 0.000289 0.001237 0.000535 0.000739 0.003098 0.000278 0.000527 0.000844 0.000534 0.000769
Nuclear Periphery Foci 0.000400 0.000623 0.000113 0.000309 0.000393 0.000395 0.001075 0.000160 0.000394 0.000421 0.000197 0.000236
Nucleolus 0.008400 0.003608 0.000361 0.000748 0.001876 0.003148 0.002782 0.000319 0.003361 0.003200 0.000572 0.000539
Nucleus 0.000259 0.000244 0.000063 0.000123 0.000132 0.000297 0.000215 0.000084 0.000161 0.000291 0.000076 0.000104
Peroxisomes 0.029956 0.024401 0.002341 0.002918 0.020204 0.026141 0.037316 0.003456 0.022423 0.025945 0.005389 0.003180
Vacuole 0.004874 0.009052 0.001350 0.006515 0.005695 0.007974 0.011240 0.002358 0.005508 0.008096 0.002212 0.004488
Vacuole Periphery 0.007870 0.011773 0.005395 0.021998 0.009339 0.009477 0.013945 0.005870 0.009004 0.009735 0.006140 0.014131

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.79 0.83 -0.42 2.78 -2.67 -2.01 -1.49 -0.78 1.56 1.08 -2.93 1.49 -0.37 3.60 -3.56
Bud Neck -2.38 2.22 0.38 2.76 -3.93 -1.06 -5.40 2.16 4.01 6.56 -1.98 2.70 1.55 4.07 -1.54
Bud Site -1.83 0.91 -1.85 0.54 -2.74 -0.98 -3.31 0.02 0.94 3.27 -1.51 0.95 -1.31 0.34 -2.13
Cell Periphery -2.61 -0.47 -4.00 -0.45 -3.89 0.36 -1.86 -1.58 -2.07 0.27 -0.96 1.71 -3.44 -3.06 -4.74
Cytoplasm -2.28 2.53 -1.28 0.29 -2.67 -1.85 -2.88 0.87 2.55 3.43 -2.39 2.96 -0.26 1.69 -2.52
Cytoplasmic Foci -0.03 4.04 3.74 5.09 -2.21 -0.35 -4.62 5.00 11.10 8.28 0.03 4.95 6.02 11.73 3.61
Eisosomes -1.12 2.20 2.85 4.18 4.81 0.33 2.71 2.41 2.45 -0.61 0.04 4.60 4.80 6.08 0.41
Endoplasmic Reticulum -2.11 3.00 -1.66 -0.53 -2.70 -2.81 -3.35 -1.78 -0.31 0.51 -3.20 3.51 -2.40 -0.65 -3.61
Endosome -1.55 1.43 -2.04 -0.51 -2.89 -2.13 -3.46 -1.77 -0.49 2.33 -2.35 0.13 -2.96 -1.52 -2.78
Golgi -0.79 2.53 0.12 1.18 -6.84 -3.63 -2.94 -1.27 2.69 2.26 -2.96 2.07 -1.87 1.54 -6.32
Lipid Particles -1.67 2.57 1.34 2.72 -3.66 -2.86 -2.52 2.69 8.78 4.99 -3.40 2.65 2.59 8.59 -0.12
Mitochondria 1.69 -4.87 -2.67 -5.54 9.19 2.88 5.94 -4.80 -12.05 -9.58 2.86 -6.47 -4.64 -11.71 4.21
Mitotic Spindle -1.86 0.18 -1.42 1.76 -1.45 -2.83 -4.10 0.19 2.94 4.12 -3.30 -2.70 -1.14 2.94 1.41
None 0.82 3.18 1.92 1.41 -4.42 2.47 1.35 5.17 5.20 3.69 2.74 5.47 4.74 3.47 -1.81
Nuclear Periphery -2.96 1.94 -2.50 0.91 -3.29 -1.67 -5.60 2.51 5.38 6.27 -2.84 -0.10 -1.48 0.45 -1.45
Nuclear Periphery Foci -0.98 1.82 0.56 1.68 -2.64 -0.04 -3.09 1.59 2.09 4.81 -0.23 1.65 1.39 2.00 -0.56
Nucleolus 0.67 1.14 1.08 2.62 -2.44 -1.75 -1.34 3.68 4.73 4.62 0.09 1.70 1.72 4.83 0.20
Nucleus 0.08 1.11 0.71 1.56 -1.25 -1.66 -2.28 0.90 1.99 2.61 -1.38 1.56 0.93 1.97 -0.75
Peroxisomes 0.45 2.63 2.57 3.20 -0.79 -1.22 -2.17 4.07 8.53 4.99 -0.76 4.23 4.84 9.23 2.64
Vacuole -1.66 1.94 -0.87 1.18 -5.29 -1.49 -2.91 2.53 4.75 5.46 -1.93 3.24 0.82 3.26 -3.71
Vacuole Periphery -1.22 1.03 -4.53 -3.37 -7.72 -0.07 -1.76 1.56 3.04 4.56 -0.39 1.61 -2.51 -3.12 -6.30
Standard name
Human Ortholog
Description Regulatory subunit of acetolactate synthase; acetolactate synthase catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Ilv6

Ilv6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ilv6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available