Standard name | |
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Human Ortholog | |
Description | Subunit of a complex with Ctf8p and Ctf18p; shares some components with Replication Factor C; required for sister chromatid cohesion and telomere length maintenance |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 0 | 1 |
Bud | 1 | 0 | 0 | 0 | 0 | 5 | 9 | 5 | 2 | 2 | 3 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 1 | 1 | 6 | 7 | 13 | 12 | 4 | 4 | 2 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 0 | 14 | 18 | 22 | 25 | 39 | 29 | 21 | 42 | 34 | 24 | 25 | 82 | 119 | 7 | 5 | 3 | 0 | 0 | 2 | 3 | 7 | 13 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | 1 | 0 |
Endosome | 0 | 0 | 1 | 1 | 3 | 2 | 2 | 7 | 1 | 5 | 3 | 2 | 1 | 2 | 3 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 2 | 5 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 2 |
Mitochondria | 83 | 9 | 8 | 17 | 29 | 66 | 142 | 122 | 102 | 119 | 110 | 184 | 3 | 2 | 1 | 2 | 7 | 7 | 0 | 0 | 6 | 1 | 8 | 7 |
Nucleus | 296 | 86 | 238 | 183 | 321 | 253 | 395 | 351 | 175 | 220 | 162 | 265 | 379 | 551 | 579 | 13 | 65 | 43 | 284 | 86 | 221 | 141 | 403 | 439 |
Nuclear Periphery | 0 | 0 | 4 | 8 | 0 | 9 | 11 | 8 | 3 | 3 | 5 | 16 | 2 | 12 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 3 | 5 | 7 | 6 | 15 | 12 | 19 | 23 | 10 | 13 | 9 | 10 | 4 | 3 | 9 | 0 | 1 | 1 | 3 | 0 | 7 | 0 | 4 | 3 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 1 | 2 | 2 | 0 | 4 | 6 | 1 | 1 | 0 | 4 | 2 | 6 | 12 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 4 | 3 |
Vac/Vac Membrane | 2 | 2 | 7 | 2 | 7 | 8 | 20 | 19 | 4 | 22 | 21 | 10 | 3 | 3 | 7 | 0 | 6 | 4 | 10 | 3 | 18 | 12 | 36 | 47 |
Unique Cell Count | 319 | 88 | 265 | 211 | 369 | 319 | 523 | 462 | 238 | 336 | 257 | 364 | 407 | 643 | 710 | 19 | 78 | 48 | 310 | 96 | 280 | 167 | 483 | 538 |
Labelled Cell Count | 391 | 102 | 282 | 238 | 407 | 388 | 657 | 583 | 323 | 433 | 350 | 536 | 419 | 662 | 740 | 24 | 89 | 61 | 310 | 96 | 280 | 167 | 483 | 538 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.1 | 6.0 | 6.0 | 5.7 | 5.1 | 5.0 | 4.7 | 4.5 | 5.0 | 4.5 | 4.1 | 6.8 | 6.5 | 6.4 | 7.8 | 7.0 | 6.8 | 5.9 | 6.3 | 6.9 |
Std Deviation (1e-4) | 0.7 | 0.8 | 2.4 | 3.5 | 2.6 | 3.9 | 3.9 | 2.3 | 2.5 | 3.5 | 2.7 | 1.9 | 1.6 | 1.4 | 1.4 | 2.1 | 1.8 | 1.2 | 1.1 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.08 | -0.22 | -0.26 | -0.35 | -0.4 | -0.26 | -0.4 | -0.55 | 0.18 | 0.13 | 0.09 | 0.39 | 0.23 | 0.19 | -0.02 | 0.08 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.4 | 0.4 | 1.2 | 1.2 | 0.5 | 1.6 | 3.0 | 3.1 | 0.7 | 0.5 | 3.3 | 4.6 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.4 | 2.6 | 6.4 | 8.2 | 0 | 0 | 9.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.0 | -1.1 | -3.5 | -4.8 | -4.6 | -4.8 | -7.0 | -7.2 | -5.3 | 1.5 | -1.7 | -3.1 | -2.8 | -1.6 | 0 |
Nuclear Periphery | 1.6 | 0 | 1.1 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.1 | 1.0 | 0.8 | 0.7 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | -0.6 | -0.1 | 0.9 | 1.0 | -0.7 | 2.2 | 2.8 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2183 | 0.9736 | 0.9023 | 0.9506 | 0.8375 | 0.93 | 0.7506 | 1.1998 | 0.9728 | 0.805 | 0.6447 | 0.8901 | -0.2671 | 0.1874 | -0.2052 | 0.4039 | 0.093 | -0.3352 |
Actin | 0.0309 | 0.0011 | 0.0045 | 0.0083 | 0.0165 | 0.0025 | 0.022 | 0.0016 | 0.0162 | 0.0037 | 0.0166 | 0.0034 | 0.0127 | 0.0001 | 0.0013 | 0.0628 | 0.0064 | 0.0003 |
Bud | 0.0007 | 0.0001 | 0.0021 | 0.0003 | 0.0006 | 0.0046 | 0.0011 | 0.0001 | 0.0007 | 0.0004 | 0.0005 | 0.0006 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 |
Bud Neck | 0.0035 | 0.0002 | 0.0004 | 0.0013 | 0.0021 | 0.0019 | 0.0141 | 0.0001 | 0.005 | 0.0005 | 0.0004 | 0.0006 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0037 | 0.0023 |
Bud Periphery | 0.0008 | 0.0001 | 0.0013 | 0.0008 | 0.0006 | 0.0013 | 0.002 | 0 | 0.0013 | 0.0006 | 0.001 | 0.0015 | 0.0002 | 0 | 0 | 0.0002 | 0.0002 | 0.0004 |
Bud Site | 0.0015 | 0.0033 | 0.0026 | 0.0013 | 0.0014 | 0.0006 | 0.0069 | 0.0015 | 0.0048 | 0.0016 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0002 |
Cell Periphery | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0135 | 0.0101 | 0.0096 | 0.0047 | 0.0044 | 0.0034 | 0.0084 | 0.0019 | 0.0037 | 0.0112 | 0.0005 | 0.0006 | 0.0043 | 0.0011 | 0.0005 | 0.0001 | 0.0026 | 0.0002 |
Cytoplasmic Foci | 0.0178 | 0.003 | 0.0037 | 0.0053 | 0.0033 | 0.0012 | 0.0099 | 0.0021 | 0.012 | 0.0097 | 0.0051 | 0.0016 | 0.0028 | 0 | 0.0003 | 0.0004 | 0.0018 | 0.0001 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0042 | 0.0001 | 0.0011 | 0.0044 | 0.0045 | 0.0008 | 0.0031 | 0.0003 | 0.0007 | 0.0021 | 0.0009 | 0.0006 | 0.0022 | 0.0001 | 0.0004 | 0.0002 | 0.0016 | 0.0001 |
Endosome | 0.0239 | 0.0007 | 0.0097 | 0.027 | 0.0266 | 0.0059 | 0.0315 | 0.0029 | 0.0109 | 0.0429 | 0.0228 | 0.0069 | 0.013 | 0.0001 | 0.0024 | 0.0013 | 0.0051 | 0.0008 |
Golgi | 0.0043 | 0.0006 | 0.0027 | 0.0028 | 0.0038 | 0.001 | 0.0079 | 0.0011 | 0.0081 | 0.01 | 0.0163 | 0.0017 | 0.0029 | 0 | 0.0003 | 0.0023 | 0.0007 | 0.0004 |
Lipid Particles | 0.0056 | 0.0003 | 0.0059 | 0.0038 | 0.0191 | 0.0013 | 0.0069 | 0.0008 | 0.0123 | 0.0127 | 0.0421 | 0.0038 | 0.0037 | 0 | 0.0002 | 0.0006 | 0.0009 | 0.0004 |
Mitochondria | 0.0097 | 0.0002 | 0.0032 | 0.0059 | 0.0045 | 0.0029 | 0.0201 | 0.0004 | 0.0183 | 0.0114 | 0.0152 | 0.0043 | 0.0042 | 0.0002 | 0.0003 | 0.001 | 0.0008 | 0.003 |
None | 0.0042 | 0.0028 | 0.0078 | 0.0011 | 0.0047 | 0.002 | 0.0158 | 0.0003 | 0.0004 | 0.0043 | 0.0005 | 0.0002 | 0.0068 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0 |
Nuclear Periphery | 0.0454 | 0.0048 | 0.0097 | 0.0173 | 0.0381 | 0.0125 | 0.0176 | 0.0028 | 0.0087 | 0.0038 | 0.0129 | 0.0039 | 0.0084 | 0.0103 | 0.0027 | 0.001 | 0.0093 | 0.0011 |
Nucleolus | 0.0155 | 0.0123 | 0.0138 | 0.0115 | 0.0172 | 0.0163 | 0.0115 | 0.0062 | 0.01 | 0.0028 | 0.0115 | 0.0089 | 0.0065 | 0.006 | 0.0094 | 0.0026 | 0.0086 | 0.0099 |
Nucleus | 0.7876 | 0.9352 | 0.9022 | 0.8878 | 0.8176 | 0.9265 | 0.7978 | 0.9728 | 0.8726 | 0.8709 | 0.8346 | 0.9557 | 0.9201 | 0.9802 | 0.9804 | 0.9254 | 0.9408 | 0.9777 |
Peroxisomes | 0.0068 | 0.0009 | 0.0014 | 0.001 | 0.0016 | 0.0004 | 0.0034 | 0.0003 | 0.0077 | 0.0031 | 0.007 | 0.002 | 0.001 | 0 | 0.0001 | 0.0006 | 0.0005 | 0.0002 |
Punctate Nuclear | 0.0173 | 0.0239 | 0.0118 | 0.0061 | 0.0275 | 0.0136 | 0.0135 | 0.0042 | 0.0039 | 0.0022 | 0.0088 | 0.0018 | 0.0084 | 0.0013 | 0.0009 | 0.0004 | 0.0146 | 0.0012 |
Vacuole | 0.0037 | 0.0003 | 0.0042 | 0.0061 | 0.0027 | 0.0008 | 0.0039 | 0.0004 | 0.0013 | 0.0038 | 0.0012 | 0.0008 | 0.0007 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.001 |
Vacuole Periphery | 0.0025 | 0.0001 | 0.0021 | 0.0032 | 0.0026 | 0.0008 | 0.0021 | 0.0002 | 0.0009 | 0.0021 | 0.0013 | 0.0006 | 0.001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.0323 | 9.4927 | 8.9651 | 8.9331 | 6.2581 | 9.6482 | 13.3064 | 10.306 | 11.6815 | 9.1355 |
Translational Efficiency | 0.7822 | 0.9539 | 0.8977 | 0.9061 | 1.4066 | 1.0072 | 0.8321 | 0.927 | 0.7579 | 1.0184 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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