Standard name
Human Ortholog
Description Protein that regulates peroxisome populations; peroxisomal integral membrane protein; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.09 0.1 0.1 0.14
Bud 0 0 0 0.06 0 0 – 0.05 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.86 0.78 0.73 0.72 0.57 – 0.62 0.59 0.54 0.46 0.47 0.87 0.86 0.85 0.76 0.77 0.76 0.54 0.4 0.31 0.4 0.42 0.42
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.11 0.05 0.09 0 0 0 0 0 0
Endosome 0 0.05 0 0 0.06 0 – 0 0 0 0 0 0 0.09 0.06 0 0 0 0.06 0.09 0.09 0.06 0 0.05
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.11 0 0.19 0.32 0.21 0.57 – 0.51 0.63 0.59 0.68 0.73 0 0 0 0.06 0 0 0.17 0.22 0.28 0.21 0.2 0.18
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0 – 0 0 0 0 0 0.05 0.06 0.09 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0.06 0 – 0 0 0 0.05 0 0.06 0.05 0.07 0.09 0.15 0.07 0 0.06 0.06 0.05 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 0 0 1 0 – 0 0 0 0 0 1 0 0 4 3 1 14 37 36 20 22 32
Bud 4 2 12 19 13 20 – 22 25 42 17 11 1 3 5 5 2 3 3 7 5 2 6 7
Bud Neck 1 2 14 2 5 0 – 5 2 7 2 0 2 2 4 2 4 1 4 12 13 9 10 11
Bud Site 0 0 5 3 0 6 – 5 6 4 1 4 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 2 1 2 2 3 – 1 5 4 4 3 2 1 1 5 2 3 2 2 6 2 1 1
Cytoplasm 160 247 294 232 305 276 – 295 389 382 149 215 169 187 246 96 75 66 108 146 122 76 90 97
Endoplasmic Reticulum 1 4 5 1 3 1 – 0 0 0 1 2 0 0 0 14 5 8 1 3 7 2 3 4
Endosome 3 13 0 1 24 2 – 3 1 3 0 0 8 19 18 3 2 1 12 30 34 11 8 10
Golgi 0 1 0 1 0 0 – 0 0 0 0 0 0 1 0 0 0 4 1 0 1 0 0 3
Mitochondria 20 8 73 102 89 277 – 244 416 420 223 336 2 0 1 7 3 3 34 80 111 40 42 41
Nucleus 0 1 0 0 1 2 – 2 1 3 0 0 0 0 1 0 2 0 0 0 1 1 0 1
Nuclear Periphery 0 0 0 1 0 0 – 1 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 1
Nucleolus 1 0 0 0 0 0 – 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 1 15 12 8 – 3 2 3 0 0 9 12 25 1 1 0 1 3 11 2 3 4
SpindlePole 4 8 14 17 25 18 – 21 15 29 16 18 12 10 20 12 15 6 5 20 23 9 11 5
Vac/Vac Membrane 0 4 0 1 6 2 – 2 3 4 1 5 1 0 0 5 0 1 8 9 8 6 7 4
Unique Cell Count 180 287 376 317 423 484 475 659 706 327 458 194 218 288 127 98 87 202 364 400 192 214 233
Labelled Cell Count 196 294 419 397 486 615 606 865 901 414 596 207 235 322 154 115 97 202 364 400 192 214 233


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.4 3.2 3.2 3.6 3.0 – 3.1 2.4 2.7 2.6 2.7 4.3 4.9 4.6 5.4 5.7 6.0 4.6 5.1 5.1
Std Deviation (1e-4) 1.3 1.0 1.1 2.2 1.4 1.4 – 1.4 0.8 0.7 0.7 1.2 0.8 2.5 1.6 1.8 2.1 2.8 2.0 2.8 2.4
Intensity Change (Log2) – – – 0.0 0.18 -0.12 – -0.05 -0.41 -0.23 -0.31 -0.28 0.41 0.59 0.5 0.75 0.82 0.9 0.53 0.67 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0849 -0.1045 -0.2293 -0.1093 -0.2946 -0.2006 -0.3693 -0.8539 -0.7889 -0.6764 -0.3174 -0.8872 0.6792 0.5509 0.6764 1.1267 0.9005 0.712
Actin 0.0314 0.0005 0.0137 0.0106 0.0032 0.0086 0.0368 0.0013 0.008 0.0038 0.0035 0.0043 0.0128 0.0006 0.0167 0.0244 0.0651 0.0031
Bud 0.0015 0.0025 0.0012 0.0016 0.0001 0.0005 0.0004 0.0043 0.0028 0.0006 0.0002 0.0051 0.0003 0.0013 0.0003 0.0052 0.0003 0.0002
Bud Neck 0.0115 0.0005 0.0098 0.0008 0.0009 0.008 0.0093 0.0013 0.0084 0.0196 0.0003 0.0048 0.0031 0.0009 0.0062 0.0178 0.0013 0.005
Bud Periphery 0.0044 0.0022 0.002 0.0054 0.0003 0.0016 0.0007 0.0021 0.0036 0.0013 0.0004 0.0018 0.0005 0.0006 0.0008 0.0219 0.0008 0.0005
Bud Site 0.0185 0.0181 0.0059 0.0026 0.0004 0.0002 0.006 0.0292 0.0266 0.0102 0.0017 0.0007 0.0067 0.0017 0.0043 0.0072 0.0003 0.0005
Cell Periphery 0.0011 0.0004 0.0003 0.0006 0.0002 0.0001 0.0004 0.0005 0.0006 0.0012 0.0001 0.0001 0.0003 0.0005 0.0007 0.0005 0.0002 0.0001
Cytoplasm 0.0782 0.1432 0.127 0.0911 0.2055 0.1454 0.0564 0.1154 0.0726 0.0597 0.0164 0.097 0.0733 0.0924 0.0634 0.0327 0.0479 0.1018
Cytoplasmic Foci 0.0893 0.0633 0.0415 0.0842 0.0441 0.1275 0.1014 0.0336 0.0537 0.1271 0.0971 0.1073 0.102 0.0254 0.0653 0.0526 0.1303 0.0852
Eisosomes 0.0007 0.0002 0.0003 0.0003 0.0001 0.0001 0.0005 0.0004 0.0003 0.0003 0.0003 0.0003 0.0007 0.0003 0.0009 0.0008 0.0018 0.0001
Endoplasmic Reticulum 0.0044 0.0014 0.0022 0.003 0.0037 0.0012 0.0038 0.0013 0.0012 0.0026 0.0007 0.0016 0.0023 0.0015 0.0009 0.0024 0.0036 0.0008
Endosome 0.0327 0.004 0.013 0.037 0.0428 0.0125 0.0379 0.0092 0.0086 0.0502 0.0853 0.0189 0.0154 0.0032 0.0112 0.0149 0.0298 0.0087
Golgi 0.0106 0.0009 0.0042 0.0114 0.0154 0.0027 0.0153 0.0021 0.0034 0.0052 0.0305 0.0073 0.0036 0.0004 0.0025 0.0076 0.0095 0.001
Lipid Particles 0.0193 0.0017 0.006 0.0096 0.0427 0.0046 0.0403 0.0021 0.0071 0.0046 0.0342 0.0086 0.013 0.0013 0.0092 0.0093 0.0145 0.0045
Mitochondria 0.0154 0.0004 0.0037 0.0299 0.0305 0.0055 0.0072 0.001 0.0037 0.0034 0.0281 0.0114 0.0015 0.001 0.0022 0.0063 0.0065 0.0011
None 0.6296 0.7529 0.7506 0.6211 0.5813 0.6645 0.5976 0.7867 0.7738 0.6589 0.6588 0.6893 0.6977 0.8625 0.7945 0.7178 0.6278 0.77
Nuclear Periphery 0.0053 0.0002 0.0019 0.002 0.0018 0.0009 0.0237 0.0004 0.0006 0.0014 0.0007 0.0008 0.0147 0.0005 0.0007 0.0043 0.0144 0.0007
Nucleolus 0.0028 0.0004 0.0003 0.0005 0.0006 0.0002 0.002 0.0005 0.0005 0.002 0.0006 0.0005 0.001 0.0008 0.0004 0.002 0.001 0.0003
Nucleus 0.0056 0.0007 0.0008 0.0011 0.0028 0.0007 0.0065 0.0032 0.0013 0.0039 0.0006 0.0013 0.0052 0.0013 0.0007 0.0078 0.0016 0.0007
Peroxisomes 0.0243 0.0037 0.0121 0.0776 0.0163 0.0116 0.0374 0.0022 0.018 0.0055 0.0269 0.0256 0.0219 0.0009 0.0149 0.0358 0.0168 0.0121
Punctate Nuclear 0.0065 0.0023 0.0015 0.0015 0.0025 0.0026 0.0108 0.0019 0.0022 0.029 0.0015 0.0121 0.0223 0.0019 0.0033 0.0261 0.0225 0.0029
Vacuole 0.0051 0.0006 0.0014 0.006 0.0041 0.0008 0.0033 0.001 0.0028 0.008 0.0072 0.0009 0.001 0.001 0.0007 0.0018 0.002 0.0006
Vacuole Periphery 0.0014 0.0001 0.0007 0.0021 0.0008 0.0003 0.0022 0.0001 0.0003 0.0017 0.0047 0.0003 0.0008 0.0001 0.0002 0.0009 0.0019 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1893 1426 367 1477 388 1871 616 1709 2281 3297 983 3186

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 659.20 706.75 818.67 790.29 574.74 689.19 788.19 780.25 644.83 696.78 799.57 784.90
Standard Deviation 72.23 83.93 83.84 111.78 66.37 80.82 97.17 115.88 78.01 82.64 93.59 114.11
Intensity Change Log 2 — 0.100484 0.312566 0.261666 — 0.261992 0.455634 0.441027 — 0.177965 0.380972 0.347988

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000760 0.001059 0.001020 0.013085 0.003849 0.001272 0.003656 0.014190 0.001285 0.001180 0.002672 0.013678
Bud Neck 0.034078 0.044000 0.006439 0.019546 0.042183 0.062388 0.020532 0.020241 0.035456 0.054435 0.015271 0.019919
Bud Site 0.007280 0.016704 0.013007 0.052771 0.005852 0.021300 0.033103 0.060871 0.007037 0.019312 0.025601 0.057116
Cell Periphery 0.000205 0.000164 0.000295 0.000437 0.000951 0.000187 0.000451 0.000309 0.000332 0.000177 0.000392 0.000368
Cytoplasm 0.338968* 0.301952* 0.243925* 0.258475* 0.124667* 0.305390* 0.292464* 0.280141* 0.302515* 0.303903* 0.274342* 0.270097*
Cytoplasmic Foci 0.355576* 0.351193* 0.072951 0.146229 0.333386* 0.328690* 0.117488 0.145796 0.351801* 0.338422* 0.100860 0.145997
Eisosomes 0.000500 0.000270 0.000147 0.000322 0.002006 0.000419 0.000220 0.000255 0.000756 0.000354 0.000193 0.000286
Endoplasmic Reticulum 0.001011 0.000545 0.003774 0.002580 0.000472 0.000655 0.003229 0.001476 0.000919 0.000608 0.003432 0.001988
Endosome 0.016675 0.029467 0.007100 0.025565 0.013204 0.033505 0.008041 0.031564 0.016084 0.031759 0.007690 0.028783
Golgi 0.008477 0.010820 0.000276 0.004432 0.016816 0.014037 0.000753 0.006943 0.009895 0.012646 0.000575 0.005779
Lipid Particles 0.020390 0.012084 0.006634 0.013293 0.075032 0.016312 0.005694 0.009153 0.029685 0.014484 0.006045 0.011072
Mitochondria 0.010278 0.021141 0.001745 0.004808 0.064175 0.023905 0.001561 0.011356 0.019446 0.022710 0.001630 0.008320
Mitotic Spindle 0.000807 0.007599 0.000932 0.047762 0.000783 0.009072 0.002864 0.051343 0.000803 0.008435 0.002143 0.049683
None 0.001935 0.002398 0.003735 0.001860 0.001904 0.001752 0.005543 0.001428 0.001929 0.002032 0.004868 0.001629
Nuclear Periphery 0.000311 0.000399 0.002033 0.001250 0.000184 0.000336 0.001145 0.000879 0.000289 0.000363 0.001476 0.001051
Nuclear Periphery Foci 0.000235 0.000279 0.001963 0.003694 0.000197 0.000237 0.001886 0.001422 0.000228 0.000255 0.001915 0.002475
Nucleolus 0.001014 0.002421 0.001858 0.001438 0.001010 0.001821 0.001675 0.000985 0.001013 0.002081 0.001743 0.001195
Nucleus 0.122359 0.086778 0.552029* 0.116348 0.041827 0.074075 0.387762* 0.072162 0.108660 0.079569 0.449091* 0.092646
Peroxisomes 0.040907 0.047939 0.009443 0.122442* 0.264487* 0.043097 0.036555 0.157970* 0.078938 0.045191 0.026433 0.141499*
Vacuole 0.037330 0.060551 0.070281 0.161313 0.006173 0.059323 0.075125 0.127324 0.032030 0.059854 0.073316 0.143081
Vacuole Periphery 0.000906 0.002237 0.000413 0.002352 0.000843 0.002226 0.000253 0.004193 0.000896 0.002231 0.000313 0.003339

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.93 -2.09 -6.63 -6.27 -6.17 3.10 0.12 -4.95 -8.22 -5.39 0.48 -3.47 -10.16 -10.25 -7.69
Bud Neck -2.95 11.19 7.64 9.27 -5.23 -3.02 3.38 3.45 13.35 -0.22 -6.87 8.17 7.99 16.28 -1.96
Bud Site -5.54 -1.45 -10.65 -6.87 -6.07 -8.12 -5.64 -12.73 -7.72 -3.74 -9.90 -5.53 -16.57 -10.25 -5.99
Cell Periphery 1.50 -2.95 -3.40 -5.31 -0.93 5.74 3.56 5.18 -3.03 3.52 4.61 -1.41 0.82 -5.94 2.53
Cytoplasm 4.01 9.50 10.53 5.86 -2.20 -16.90 -10.26 -13.67 3.21 -1.43 -0.25 6.37 5.59 6.36 -2.10
Cytoplasmic Foci 0.59 29.92 31.74 27.65 -6.36 0.65 16.58 16.67 27.06 -2.79 2.26 34.03 37.31 38.63 -5.58
Eisosomes 5.37 7.78 4.95 -0.60 -4.41 3.42 3.80 3.83 5.46 0.52 4.58 6.16 5.79 4.26 -1.94
Endoplasmic Reticulum 1.96 -8.78 -7.39 -13.55 4.85 -1.27 -13.23 -7.03 -5.00 8.57 1.50 -12.66 -6.47 -12.28 9.13
Endosome -6.70 4.94 -1.72 4.46 -5.92 -8.67 2.58 -4.86 3.42 -8.08 -10.39 6.25 -4.29 5.44 -10.00
Golgi -1.68 12.15 7.07 6.92 -4.80 1.62 10.91 7.49 7.64 -5.52 -2.72 15.14 8.24 10.38 -7.09
Lipid Particles 5.49 8.48 4.95 -0.37 -3.50 8.60 10.02 9.68 5.16 -2.48 8.61 13.20 11.04 3.76 -4.36
Mitochondria -5.31 7.91 6.36 9.40 -2.21 5.68 9.10 8.01 8.07 -5.89 -1.65 12.15 8.45 12.26 -6.28
Mitotic Spindle -4.07 -1.46 -10.88 -8.18 -10.43 -5.83 -3.16 -12.83 -9.87 -10.56 -6.97 -3.39 -16.78 -12.80 -14.40
None -0.90 -2.82 0.20 0.92 2.62 0.16 -1.75 0.55 1.08 2.37 -0.30 -2.58 0.90 1.31 2.95
Nuclear Periphery -1.86 -14.41 -13.40 -11.86 3.41 -2.19 -14.83 -10.78 -8.16 1.47 -1.72 -19.75 -16.38 -13.81 2.89
Nuclear Periphery Foci -1.37 -5.78 -6.64 -6.56 -0.77 -0.69 -7.88 -5.08 -4.90 3.05 -0.76 -9.68 -8.25 -8.13 1.08
Nucleolus -3.60 -3.92 -2.94 0.28 0.45 -1.94 -1.86 -0.79 1.61 1.69 -3.91 -2.64 -3.07 1.16 1.60
Nucleus 7.01 -24.32 -1.67 -7.28 23.19 -5.93 -26.07 -7.98 -3.35 23.11 7.73 -31.46 -0.14 -7.81 31.36
Peroxisomes -1.83 8.51 -7.93 -6.64 -13.26 15.54 15.92 9.55 -12.50 -12.54 8.55 12.26 -4.63 -13.92 -16.88
Vacuole -8.80 -8.52 -29.67 -25.14 -13.38 -23.66 -15.19 -32.05 -21.43 -8.58 -14.38 -14.44 -39.95 -32.83 -15.32
Vacuole Periphery -5.91 3.51 -1.33 2.26 -2.87 -5.53 4.71 -3.58 -0.94 -4.90 -7.62 6.08 -3.68 0.32 -5.74
Standard name
Human Ortholog
Description Protein that regulates peroxisome populations; peroxisomal integral membrane protein; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Pex34

Pex34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available