Standard name | |
---|---|
Human Ortholog | |
Description | Putative ATP-dependent permease of the ABC transporter family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 21 | 3 | 29 | 29 | 19 | 21 | 18 | 41 | – | 30 | 17 | 20 | 5 | 5 | 6 | 23 | 28 | 19 | 2 | 2 | 4 | 0 | 1 | 1 |
Bud | 4 | 0 | 1 | 3 | 1 | 1 | 4 | 22 | – | 19 | 11 | 11 | 1 | 0 | 1 | 3 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 5 | 7 | 10 | 18 | 23 | 17 | – | 33 | 23 | 32 | 1 | 2 | 13 | 33 | 23 | 21 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 79 | 39 | 43 | 56 | 52 | 92 | 109 | 143 | – | 182 | 143 | 169 | 33 | 50 | 74 | 120 | 131 | 100 | 6 | 2 | 3 | 2 | 4 | 5 |
Endoplasmic Reticulum | 201 | 102 | 229 | 151 | 145 | 212 | 238 | 238 | – | 150 | 72 | 164 | 102 | 140 | 119 | 6 | 13 | 6 | 347 | 137 | 275 | 177 | 177 | 190 |
Endosome | 9 | 0 | 10 | 10 | 10 | 2 | 5 | 10 | – | 3 | 3 | 3 | 1 | 2 | 2 | 3 | 2 | 5 | 5 | 2 | 2 | 2 | 10 | 14 |
Golgi | 9 | 1 | 15 | 18 | 16 | 4 | 8 | 4 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 12 | 2 | 1 | 3 | 3 | 2 | 4 |
Mitochondria | 1 | 1 | 1 | 10 | 8 | 21 | 29 | 41 | – | 68 | 79 | 61 | 0 | 0 | 0 | 14 | 23 | 8 | 3 | 2 | 3 | 6 | 2 | 4 |
Nucleus | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 1 | – | 2 | 3 | 0 | 1 | 1 | 1 | 5 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 4 | 2 | 0 | 0 | 2 | 8 | – | 2 | 1 | 6 | 1 | 0 | 1 | 1 | 3 | 0 | 2 | 1 | 1 | 1 | 2 | 2 |
Nucleolus | 3 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | – | 3 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 65 | 5 | 15 | 10 | 32 | 30 | 50 | 46 | – | 17 | 9 | 17 | 9 | 16 | 6 | 18 | 16 | 19 | 13 | 4 | 4 | 19 | 19 | 46 |
Unique Cell Count | 339 | 140 | 294 | 238 | 236 | 340 | 404 | 471 | 416 | 300 | 399 | 143 | 192 | 186 | 197 | 212 | 174 | 390 | 158 | 304 | 217 | 226 | 281 | |
Labelled Cell Count | 394 | 152 | 354 | 299 | 294 | 404 | 486 | 572 | 511 | 361 | 485 | 154 | 218 | 224 | 226 | 249 | 195 | 390 | 158 | 304 | 217 | 226 | 281 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.3 | 12.5 | 12.1 | 12.2 | 11.7 | 10.8 | 10.8 | 10.4 | – | 9.8 | 9.7 | 10.0 | 12.1 | 12.0 | 12.8 | 9.2 | 10.7 | 13.5 | 12.8 | 12.7 | 13.2 |
Std Deviation (1e-4) | 2.1 | 2.4 | 1.9 | 2.2 | 2.5 | 1.9 | 1.9 | 1.8 | – | 2.1 | 1.9 | 2.0 | 1.9 | 1.8 | 2.4 | 1.7 | 2.2 | 2.6 | 1.8 | 2.0 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.05 | -0.17 | -0.16 | -0.22 | – | -0.3 | -0.31 | -0.27 | 0.0 | -0.02 | 0.08 | -0.39 | -0.18 | 0.15 | 0.08 | 0.07 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.9 | -0.7 | -1.7 | -2.8 | -0.5 | – | -1.3 | -1.9 | -2.5 | -2.3 | -3.1 | -2.7 | 0.6 | 1.2 | 0.4 |
Bud | 0 | 0 | 0 | 0 | 3.4 | – | 3.4 | 2.9 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.0 | 1.8 | 2.4 | 2.7 | 1.5 | – | 3.6 | 3.4 | 3.7 | 0 | 0 | 3.0 | 6.1 | 4.4 | 4.7 |
Cytoplasm | 2.6 | 2.2 | 3.8 | 3.9 | 4.9 | – | 8.2 | 8.7 | 7.8 | 2.2 | 3.1 | 6.3 | 10.7 | 11.0 | 9.7 |
Endoplasmic Reticulum | -3.7 | -4.1 | -4.2 | -5.3 | -7.5 | – | -11.0 | -13.1 | -9.7 | -1.5 | -1.3 | -3.3 | -16.3 | -15.9 | -15.6 |
Endosome | 0.5 | 0.5 | -2.6 | -1.9 | -1.1 | – | -2.6 | -2.0 | -2.5 | 0 | 0 | 0 | -1.3 | -1.8 | -0.3 |
Golgi | 1.2 | 0.8 | -2.9 | -2.3 | -3.7 | – | -4.3 | -4.0 | -4.6 | 0 | -2.8 | -3.1 | -3.2 | -2.9 | 0.8 |
Mitochondria | 0 | 0 | 4.0 | 4.4 | 4.9 | – | 7.1 | 9.3 | 6.8 | 0 | 0 | 0 | 4.3 | 5.5 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.5 | 3.4 | 1.8 | 3.3 | 2.3 | – | -0.6 | -1.3 | -0.5 | 0.5 | 1.4 | -1.0 | 1.8 | 1.1 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0367 | 0.0002 | 0.0022 | 0.0318 | 0.0183 | 0.0004 | 0.0258 | 0.0002 | 0.0026 | 0.0001 | 0.0005 | 0.0062 | 0.3376 | 0 | 0.0504 | 0.0001 | 0.0002 | 0.0001 |
Bud | 0.0006 | 0.0001 | 0.0007 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0023 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0054 | 0.0004 | 0.0042 | 0.0007 | 0.001 | 0.0011 | 0.0004 | 0.0004 | 0.0003 | 0.0005 | 0.0005 | 0.0002 | 0.0033 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0002 |
Bud Periphery | 0.001 | 0.0003 | 0.0011 | 0.0007 | 0.0006 | 0.0003 | 0.0009 | 0.0001 | 0.0004 | 0.0003 | 0.0007 | 0.0002 | 0.0022 | 0 | 0.0005 | 0.0004 | 0.0004 | 0.0001 |
Bud Site | 0.0076 | 0.0004 | 0.0107 | 0.0012 | 0.0018 | 0.0003 | 0.0009 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0058 | 0 | 0.0008 | 0.0001 | 0.0002 | 0 |
Cell Periphery | 0.0018 | 0.0061 | 0.0031 | 0.0035 | 0.0018 | 0.0006 | 0.0035 | 0.0035 | 0.002 | 0.0029 | 0.0013 | 0.0007 | 0.0006 | 0.0023 | 0.0032 | 0.0008 | 0.0009 | 0.0004 |
Cytoplasm | 0.0359 | 0.1342 | 0.0905 | 0.097 | 0.0917 | 0.0558 | 0.0173 | 0.0397 | 0.0317 | 0.0084 | 0.0129 | 0.0276 | 0.0034 | 0.0137 | 0.0273 | 0.0146 | 0.0146 | 0.0321 |
Cytoplasmic Foci | 0.0337 | 0.0056 | 0.0115 | 0.0111 | 0.0248 | 0.004 | 0.0173 | 0.0032 | 0.008 | 0.0013 | 0.0036 | 0.0021 | 0.0424 | 0.0003 | 0.007 | 0.0017 | 0.0034 | 0.0011 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0014 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.5559 | 0.5311 | 0.6189 | 0.4761 | 0.4778 | 0.6431 | 0.6519 | 0.8158 | 0.7798 | 0.7685 | 0.5386 | 0.7579 | 0.0076 | 0.7888 | 0.696 | 0.5101 | 0.6879 | 0.7973 |
Endosome | 0.0721 | 0.0395 | 0.0418 | 0.0595 | 0.1015 | 0.0455 | 0.0935 | 0.0334 | 0.0324 | 0.0405 | 0.0825 | 0.0276 | 0.1581 | 0.0083 | 0.0684 | 0.075 | 0.1089 | 0.0399 |
Golgi | 0.0129 | 0.0014 | 0.008 | 0.0051 | 0.0086 | 0.0019 | 0.0189 | 0.0026 | 0.004 | 0.0028 | 0.0107 | 0.0036 | 0.3339 | 0.0001 | 0.0044 | 0.0014 | 0.006 | 0.0008 |
Lipid Particles | 0.0309 | 0.026 | 0.0098 | 0.0133 | 0.0131 | 0.0035 | 0.0342 | 0.0456 | 0.0065 | 0.0184 | 0.0108 | 0.0098 | 0.0299 | 0.0025 | 0.037 | 0.0036 | 0.0098 | 0.0019 |
Mitochondria | 0.0053 | 0.0005 | 0.0163 | 0.001 | 0.0017 | 0.0009 | 0.0093 | 0.001 | 0.024 | 0.0007 | 0.0903 | 0.0007 | 0.0516 | 0.0001 | 0.0009 | 0.0013 | 0.0025 | 0.0007 |
None | 0.0132 | 0.0004 | 0.0009 | 0.01 | 0.001 | 0.0003 | 0.0132 | 0.0002 | 0.0127 | 0.0001 | 0.0002 | 0.0004 | 0.0012 | 0 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Nuclear Periphery | 0.0776 | 0.0434 | 0.0405 | 0.0441 | 0.0682 | 0.1081 | 0.0316 | 0.0159 | 0.027 | 0.0221 | 0.0528 | 0.0631 | 0.0027 | 0.021 | 0.0168 | 0.0999 | 0.0542 | 0.046 |
Nucleolus | 0.0021 | 0.0001 | 0.0004 | 0.0003 | 0.0008 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.026 | 0.0042 | 0.0031 | 0.0048 | 0.0057 | 0.0087 | 0.0023 | 0.0009 | 0.0013 | 0.0009 | 0.0014 | 0.0019 | 0.0005 | 0.0012 | 0.0008 | 0.003 | 0.0016 | 0.0014 |
Peroxisomes | 0.0082 | 0.0001 | 0.0045 | 0.0153 | 0.014 | 0.0001 | 0.0065 | 0 | 0.0009 | 0 | 0.0001 | 0.0014 | 0.0072 | 0 | 0.0035 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.0066 | 0.0003 | 0.0005 | 0.0015 | 0.0014 | 0.0006 | 0.0025 | 0.0003 | 0.0024 | 0.0001 | 0.0001 | 0.0006 | 0.0012 | 0 | 0.0005 | 0.0002 | 0.0002 | 0.0001 |
Vacuole | 0.0585 | 0.1912 | 0.1089 | 0.2006 | 0.1527 | 0.1045 | 0.0626 | 0.0333 | 0.0587 | 0.1245 | 0.1316 | 0.0841 | 0.0029 | 0.154 | 0.0766 | 0.2367 | 0.0925 | 0.0658 |
Vacuole Periphery | 0.0076 | 0.0143 | 0.0221 | 0.0221 | 0.0132 | 0.0201 | 0.0066 | 0.0031 | 0.005 | 0.0076 | 0.0611 | 0.0115 | 0.0038 | 0.0073 | 0.0051 | 0.0506 | 0.0158 | 0.012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 37.1304 | 41.647 | 44.3807 | 59.6046 | 39.058 | 35.7095 | 64.7469 | 69.7701 | 66.425 | 63.2251 |
Translational Efficiency | 0.8185 | 0.7484 | 0.7799 | 0.628 | 0.716 | 0.9506 | 0.7789 | 0.5178 | 0.6072 | 0.5844 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|