Standard name
Human Ortholog
Description Negative regulator of the H(+)-ATPase Pma1p; stress-responsive protein; hydrophobic plasma membrane localized; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0.07 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 – 0.06 0.08 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0.09 0.11 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.94 0.98 0.96 0.91 0.84 0.74 – 0.63 0.62 0.56 0.94 0.98 0.98 0.89 0.93 0.93 0.82 0.77 0.72 0.58 0.51 0.57
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.09 0 0.07 0.18 0.28 0.25 – 0.21 0.31 0.31 0 0 0 0.07 0 0 0.08 0 0.08 0.07 0.06 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.13 0.05 0.23 0.31 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 4 14 – 45 37 50 0 1 4 1 2 0 2 1 2 0 3 4
Bud 2 0 1 0 3 17 18 38 – 41 41 65 0 1 2 3 2 0 0 1 0 2 6 6
Bud Neck 1 0 2 2 13 15 14 13 – 4 8 9 0 5 1 2 1 1 1 1 1 0 3 4
Bud Site 0 0 0 0 0 1 1 5 – 8 7 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 2 1 4 11 19 – 57 57 74 0 1 4 3 5 2 0 0 0 0 6 3
Cytoplasm 169 142 139 316 470 500 670 599 – 411 309 365 16 296 369 160 154 139 140 110 104 57 158 150
Endoplasmic Reticulum 0 0 0 0 0 2 1 5 – 7 3 11 0 0 1 5 14 14 0 0 1 2 3 4
Endosome 1 0 0 2 1 0 0 1 – 2 0 1 0 1 0 2 0 1 3 0 3 0 2 3
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 2 0 1 0 0 1 0
Mitochondria 0 3 14 7 33 98 227 202 – 137 153 202 0 0 0 12 2 3 13 4 12 7 17 9
Nucleus 0 0 0 2 1 1 3 8 – 6 5 8 1 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 8 19 – 31 23 24 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 2 – 0 2 4 0 0 0 0 1 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 6 – 4 2 4 0 1 0 0 1 1 0 0 0 0 0 0
SpindlePole 6 0 0 2 2 4 13 29 – 35 23 37 0 0 1 4 1 1 1 0 2 1 1 4
Vac/Vac Membrane 0 4 2 0 2 0 5 12 – 27 13 20 0 4 1 2 2 1 3 18 6 22 94 53
Unique Cell Count 173 148 148 321 489 550 797 807 652 499 657 17 302 375 179 166 149 171 144 145 100 308 263
Labelled Cell Count 181 149 158 333 526 644 975 972 815 683 884 17 310 383 195 185 165 171 144 145 100 308 263


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.6 3.0 2.9 3.0 3.0 2.5 3.3 – 4.6 5.4 5.4 4.4 4.6 4.8 5.1 5.2 5.1 5.6 6.3 6.0
Std Deviation (1e-4) 2.1 3.3 1.0 1.2 1.3 1.3 1.3 2.0 – 4.9 8.2 6.9 0.8 1.5 1.5 2.1 2.9 1.6 7.2 7.6 6.0
Intensity Change (Log2) – – – -0.05 -0.04 -0.04 -0.29 0.12 – 0.59 0.84 0.84 0.52 0.61 0.65 0.76 0.77 0.76 0.89 1.04 0.99


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.054 0.6594 -0.0177 0.2564 0.2472 0.3316 0.421 2.3084 1.345 1.7447 0.8707 1.6114 -1.6159 -0.817 -1.1044 -1.0442 -0.6687 -1.2034
Actin 0.0167 0.0009 0.0055 0.0007 0.0021 0.0011 0.0072 0.0004 0.0015 0.0005 0.0033 0.0032 0.0189 0.0001 0.0092 0.0342 0.0054 0.0006
Bud 0.0055 0.0116 0.0084 0.0009 0.0106 0.0035 0.0032 0.0061 0.0046 0.0156 0.0013 0.0032 0.0011 0.0011 0.0019 0.0017 0.0041 0.0026
Bud Neck 0.0022 0.0016 0.0009 0.0007 0.0032 0.0027 0.0029 0.0054 0.0018 0.0025 0.0018 0.0025 0.0009 0.0008 0.0004 0.0013 0.0009 0.0011
Bud Periphery 0.0072 0.0126 0.008 0.0017 0.0143 0.006 0.0109 0.0158 0.0117 0.0346 0.0045 0.0069 0.0018 0.0018 0.0036 0.0021 0.0329 0.0149
Bud Site 0.0105 0.0288 0.0083 0.0012 0.0221 0.0018 0.0039 0.0112 0.0061 0.0041 0.0018 0.0008 0.0016 0.0066 0.004 0.007 0.003 0.0016
Cell Periphery 0.0012 0.0036 0.0019 0.0005 0.0038 0.002 0.0037 0.0059 0.0072 0.0032 0.0023 0.0029 0.0009 0.0019 0.0018 0.0005 0.0214 0.0039
Cytoplasm 0.0392 0.0445 0.0465 0.0533 0.0691 0.0759 0.0434 0.0823 0.047 0.0468 0.0172 0.0787 0.0727 0.0724 0.0878 0.0729 0.0903 0.0941
Cytoplasmic Foci 0.0636 0.0226 0.0441 0.0295 0.0535 0.0462 0.0405 0.0288 0.0279 0.0688 0.016 0.0379 0.0489 0.0311 0.0482 0.043 0.0182 0.028
Eisosomes 0.0005 0.0003 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0032 0.0031 0.002 0.002 0.0038 0.0024 0.0052 0.0062 0.0034 0.0029 0.0016 0.0061 0.004 0.0047 0.0033 0.002 0.0009 0.0034
Endosome 0.0902 0.0627 0.098 0.0942 0.099 0.0817 0.1149 0.0893 0.1166 0.1378 0.1333 0.0803 0.1027 0.057 0.1457 0.1626 0.0766 0.0917
Golgi 0.0099 0.002 0.0081 0.0051 0.007 0.0036 0.0049 0.0024 0.0052 0.0106 0.0072 0.0039 0.0068 0.0008 0.003 0.01 0.0306 0.0015
Lipid Particles 0.0329 0.0117 0.0219 0.021 0.016 0.0148 0.0246 0.0141 0.0138 0.0163 0.0197 0.0097 0.0214 0.0039 0.0102 0.0266 0.0118 0.0172
Mitochondria 0.023 0.0077 0.0182 0.005 0.0081 0.0069 0.025 0.0076 0.0373 0.0174 0.0607 0.0106 0.0084 0.0086 0.0245 0.0035 0.034 0.008
None 0.5171 0.5594 0.5678 0.5299 0.36 0.4859 0.4832 0.3078 0.4659 0.2569 0.3196 0.4366 0.5107 0.5073 0.4846 0.3935 0.4339 0.4022
Nuclear Periphery 0.0092 0.0029 0.0035 0.003 0.0069 0.0043 0.0046 0.0085 0.0049 0.0049 0.0022 0.0026 0.0112 0.0075 0.0042 0.0028 0.0006 0.0132
Nucleolus 0.0055 0.0075 0.0058 0.0056 0.0078 0.0046 0.0036 0.0063 0.005 0.0138 0.0071 0.0029 0.002 0.0022 0.0031 0.0024 0.001 0.0019
Nucleus 0.0121 0.0096 0.0106 0.0057 0.0247 0.0114 0.0046 0.0088 0.0046 0.0127 0.0057 0.0055 0.0059 0.0028 0.0055 0.0031 0.0009 0.0045
Peroxisomes 0.0249 0.0015 0.017 0.0083 0.008 0.0076 0.0121 0.0018 0.0052 0.0099 0.0026 0.0037 0.0239 0.0004 0.0039 0.0058 0.0068 0.0109
Punctate Nuclear 0.0091 0.0022 0.0084 0.0092 0.0075 0.0074 0.0014 0.0013 0.0018 0.0304 0.0006 0.0014 0.0023 0.0017 0.0053 0.0099 0.0002 0.0026
Vacuole 0.1011 0.1751 0.0972 0.1971 0.2424 0.2036 0.1707 0.3431 0.1859 0.2452 0.3442 0.2602 0.1198 0.2198 0.1214 0.1768 0.1854 0.2626
Vacuole Periphery 0.0152 0.028 0.0176 0.0253 0.0299 0.0263 0.0292 0.0471 0.0425 0.0649 0.0468 0.0401 0.0341 0.0674 0.0281 0.038 0.0409 0.0333

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.6568 5.9242 3.6633 1.935 3.5801 2.2078 2.7454 1.0107 3.1655 3.2884
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2644 2061 1728 694 1742 1765 1694 1712 4386 3826 3422 2406

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 631.55 746.93 1421.55 1186.61 645.07 655.04 811.24 1057.56 636.92 704.54 1119.43 1094.78
Standard Deviation 64.92 108.49 663.04 580.27 77.48 87.55 98.20 709.58 70.49 109.43 565.58 677.35
Intensity Change Log 2 — 0.242076 1.170496 0.909877 — 0.022127 0.330673 0.713212 — 0.135126 0.806518 0.813853

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000130 0.000855 0.056583 0.038291 0.000166 0.001229 0.002988 0.039668 0.000144 0.001027 0.030052 0.039271
Bud Neck 0.021921 0.033482 0.078956 0.024260 0.024026 0.055919 0.007286 0.037436 0.022757 0.043833 0.043477 0.033635
Bud Site 0.010958 0.021231 0.243732* 0.214488* 0.005219 0.017754 0.018749 0.244967* 0.008678 0.019627 0.132358* 0.236176*
Cell Periphery 0.000151 0.001812 0.198204* 0.377415* 0.000117 0.000441 0.007373 0.301823* 0.000138 0.001179 0.103736* 0.323627*
Cytoplasm 0.475729* 0.423982* 0.057340 0.141809* 0.442578* 0.328882* 0.390695* 0.202409* 0.462562* 0.380111* 0.222362* 0.184929*
Cytoplasmic Foci 0.180903* 0.183431* 0.020994 0.019344 0.256172* 0.321794* 0.017043 0.014626 0.210798* 0.247260* 0.019038 0.015987
Eisosomes 0.000154 0.000256 0.008779 0.015462 0.000185 0.000449 0.001072 0.016685 0.000166 0.000345 0.004964 0.016332
Endoplasmic Reticulum 0.000981 0.001242 0.067523 0.007481 0.000563 0.000447 0.002144 0.003161 0.000815 0.000875 0.035158 0.004407
Endosome 0.007786 0.018884 0.044761 0.006910 0.018486 0.027007 0.001536 0.002835 0.012035 0.022631 0.023363 0.004010
Golgi 0.002062 0.002863 0.010857 0.003876 0.002814 0.010205 0.000191 0.002211 0.002361 0.006250 0.005577 0.002692
Lipid Particles 0.010141 0.005117 0.079004 0.007459 0.012241 0.014159 0.001729 0.001375 0.010975 0.009288 0.040750 0.003130
Mitochondria 0.006927 0.005867 0.003431 0.002447 0.007242 0.028810 0.000637 0.002981 0.007052 0.016451 0.002048 0.002827
Mitotic Spindle 0.002048 0.002491 0.003982 0.003052 0.000878 0.004774 0.004258 0.001848 0.001583 0.003544 0.004118 0.002195
None 0.003571 0.002690 0.000677 0.000393 0.003067 0.003588 0.003546 0.000854 0.003371 0.003104 0.002097 0.000721
Nuclear Periphery 0.000462 0.000321 0.001208 0.000282 0.000404 0.000257 0.001054 0.000141 0.000439 0.000291 0.001132 0.000181
Nuclear Periphery Foci 0.000164 0.000310 0.003248 0.001133 0.000219 0.000198 0.000430 0.000342 0.000186 0.000258 0.001853 0.000570
Nucleolus 0.001130 0.001336 0.000247 0.000112 0.001205 0.001552 0.000414 0.000096 0.001160 0.001436 0.000330 0.000100
Nucleus 0.225857* 0.132162* 0.014488 0.042920 0.151781* 0.092051 0.500621* 0.061368 0.196436* 0.113658 0.255140* 0.056047
Peroxisomes 0.004921 0.007646 0.003275 0.001562 0.008293 0.036180 0.000622 0.001317 0.006260 0.020809 0.001962 0.001388
Vacuole 0.043213 0.151782 0.100263 0.089123 0.062795 0.051905 0.037386 0.063284 0.050990 0.105707 0.069137 0.070737
Vacuole Periphery 0.000792 0.002240 0.002448 0.002182 0.001548 0.002400 0.000225 0.000574 0.001092 0.002314 0.001347 0.001038

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.08 -17.22 -10.13 -9.90 4.34 -1.98 -3.73 -14.63 -13.90 -13.32 -3.18 -16.99 -17.72 -17.13 -2.64
Bud Neck -4.95 -17.63 -0.21 3.79 15.31 -9.75 9.48 -4.40 6.05 -13.82 -10.79 -10.42 -4.91 5.39 4.89
Bud Site -4.55 -32.25* -16.98 -15.99 3.47 -7.77 -4.82 -30.42* -28.36* -27.87* -7.48 -28.47 -34.34 -32.36 -12.01
Cell Periphery -4.24 -28.19* -21.69 -21.57 -8.92 -3.49 -4.47 -29.72* -29.68* -28.81* -4.84 -25.97 -36.68 -36.54* -22.21
Cytoplasm 6.39 65.68* 32.32 25.35 -9.27 12.51 8.43 24.89 12.60 16.02 13.63 41.10 40.47 26.46 2.27
Cytoplasmic Foci -0.42 37.50 36.13 32.30 0.23 -8.49 44.32 44.18 53.50 0.50 -7.46 57.33 57.87 59.55 1.25
Eisosomes -1.29 -10.85 -9.69 -9.60 -3.52 -3.59 -2.24 -11.74 -11.52 -10.91 -3.31 -10.78 -14.69 -14.49 -9.49
Endoplasmic Reticulum -1.39 -19.12 -7.20 -6.90 16.55 1.95 -7.89 -7.28 -7.59 -1.77 -0.55 -18.70 -9.72 -9.55 16.27
Endosome -9.29 -15.19 0.91 7.09 13.83 -4.54 13.45 11.62 16.52 -1.90 -10.10 -7.84 10.36 18.37 14.17
Golgi -2.70 -7.46 -1.22 -0.55 4.29 -8.17 6.13 0.97 7.89 -2.83 -8.81 -4.96 -0.16 5.41 3.55
Lipid Particles 6.15 -14.99 1.78 -1.21 14.96 -1.68 11.98 12.30 14.92 1.27 2.30 -12.03 10.80 9.72 15.55
Mitochondria 1.14 4.27 4.53 3.69 1.72 -8.84 5.74 3.46 11.61 -3.51 -7.64 7.62 5.68 11.83 -0.87
Mitotic Spindle -0.65 -2.97 -1.34 -0.78 1.45 -3.87 -2.89 -1.81 2.54 1.77 -3.01 -3.91 -1.44 1.63 2.72
None 1.83 7.25 8.05 7.71 2.53 -0.69 -0.53 3.41 4.59 7.56 0.50 3.53 7.27 7.19 6.64
Nuclear Periphery 2.65 -8.63 2.07 -0.22 9.88 4.58 -17.14 7.00 3.83 23.89* 4.42 -15.93 6.62 2.64 22.02
Nuclear Periphery Foci -4.20 -10.50 -4.67 -4.08 5.00 0.90 -3.06 -4.51 -5.50 1.67 -3.16 -10.83 -6.17 -5.16 7.20
Nucleolus -1.08 10.22 14.45 7.50 3.89 -1.91 4.39 7.69 10.83 4.21 -2.21 9.04 14.82 12.50 5.63
Nucleus 18.18 49.86 27.45 12.70 -6.28 12.01 -44.62 14.16 3.55 54.26* 22.51 -11.36 30.96 11.57 30.79
Peroxisomes -3.76 3.05 7.06 9.84 3.53 -13.10 10.41 9.36 17.45 -3.15 -13.55 9.92 11.99 19.35 2.12
Vacuole -19.74 -15.03 -9.62 2.37 -2.25 3.30 3.97 -4.49 -7.21 -7.64 -15.90 -9.65 -11.35 1.74 -4.29
Vacuole Periphery -8.49 -7.27 -1.78 0.36 0.63 -1.58 2.95 2.12 5.54 -2.12 -4.99 -1.11 0.46 4.95 1.49
Standard name
Human Ortholog
Description Negative regulator of the H(+)-ATPase Pma1p; stress-responsive protein; hydrophobic plasma membrane localized; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
Localization
Cell Percentages cytoplasm (14%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hsp30

Hsp30


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsp30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available