ORF
Human Ortholog
Description Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.08 0.13 0.49 0.5 0.62 0.59 0.61 0.69 0.63 0.65 0.6 0.17 0.13 0.13 0.29 0.24 0.21 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0.05 0.24 0.2 0.19 0.06 0 0.05 0.05 0 0 0 0.12 0.09 0.07 0.13 0.06 0.08 0.08 0.11 0.08 0.12 0.12 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.16 0.1 0.37 0.48 0.39 0.39 0.52 0.48 0.47 0 0 0 0.05 0.09 0 0 0 0 0 0 0
Nucleus 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0.07 0.11 0.13 0 0.12 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.84 0.84 0.8 0.41 0.5 0.28 0.19 0.18 0.15 0.15 0.14 0.21 0.76 0.8 0.79 0.61 0.68 0.74 0.82 0.73 0.78 0.77 0.77 0.73
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 1 0 0 0 0 1 0 0 2
Bud 0 0 1 3 0 3 6 14 15 7 9 14 2 1 0 1 0 0 0 0 0 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 1 0 0 1 7 6 2 6 10 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 27 15 46 168 183 318 378 486 336 364 399 415 120 94 106 86 48 26 2 2 5 2 3 4
Endoplasmic Reticulum 3 1 0 1 1 1 2 10 3 3 1 4 19 7 14 10 9 5 0 0 2 1 1 1
Endosome 18 9 87 69 70 31 20 38 23 10 11 30 87 66 55 39 12 10 27 22 32 27 47 49
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 5 0 2 2 1 1
Mitochondria 14 7 26 55 36 191 308 314 193 301 295 326 2 1 0 14 19 4 3 2 7 6 7 14
Nucleus 28 12 5 0 5 8 4 3 4 2 2 7 52 79 106 12 24 9 7 7 2 2 6 4
Nuclear Periphery 1 8 4 0 1 0 4 2 1 0 0 0 2 1 3 5 5 1 7 11 26 8 16 10
Nucleolus 5 3 7 1 0 0 1 2 0 0 0 1 0 1 1 1 0 0 0 3 0 1 0 1
Peroxisomes 0 0 1 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 1 0 3 3 1 2 1 1 1 0 0 3 4 5 2 3 0 0 0 0 0 0 0 1
Vac/Vac Membrane 267 154 287 141 183 144 125 146 74 89 87 144 551 590 636 179 138 93 278 149 305 186 305 275
Unique Cell Count 318 183 360 341 368 515 645 797 490 575 615 689 724 738 808 292 204 125 339 206 392 242 397 378
Labelled Cell Count 366 209 468 442 480 698 851 1024 656 778 810 955 840 848 926 351 255 148 339 206 392 242 397 378


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 6.7 5.8 5.4 5.4 4.5 4.2 4.5 4.8 4.1 4.5 4.5 6.6 7.1 7.3 5.7 6.2 7.0 7.0 7.1 7.3
Std Deviation (1e-4) 1.0 1.2 1.4 1.3 1.5 1.4 1.1 1.1 2.2 0.8 1.2 0.9 0.9 0.9 0.9 1.2 1.3 1.5 1.8 1.6 1.7
Intensity Change (Log2) – – – -0.11 -0.12 -0.39 -0.46 -0.37 -0.28 -0.49 -0.38 -0.37 0.19 0.28 0.33 -0.02 0.09 0.27 0.28 0.29 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 10.5 10.7 14.5 14.1 15.2 16.2 15.2 15.8 14.7 1.6 0 0.2 5.3 3.3 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0
Endosome -1.3 -1.7 0 0 0 0 0 0 0 -5.1 -6.8 -8.4 -3.5 -5.5 -3.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.7 1.2 0 0 0 0 0 0 0 -6.8 -7.1 -7.7 -1.3 0.9 -1.6
Nucleus 0 0 0.2 0 0 0 0 0 0 4.0 5.5 6.3 2.2 5.4 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -10.4 -8.5 -15.1 -18.6 -20.0 -18.8 -19.5 -20.3 -18.4 -1.3 0.1 -0.4 -5.2 -3.2 -1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9221 2.196 2.3049 2.2586 2.6724 2.3056 3.2299 3.4947 3.087 3.6814 2.9873 3.086 3.9115 4.1633 3.9678 3.8879 3.7497 4.1703
Actin 0.0019 0.0001 0.0019 0.0182 0.0032 0.0011 0.0061 0.0001 0.0001 0.0087 0 0.0163 0.0055 0.0001 0.0004 0.0003 0 0
Bud 0.0004 0.0042 0.0015 0.0027 0.0021 0.0012 0.0008 0.0001 0.0016 0.0002 0.0005 0.0004 0.0003 0.0016 0.002 0.002 0.0006 0.0008
Bud Neck 0.0011 0.0007 0.0008 0.0019 0.0003 0.0021 0.012 0.0002 0.0002 0.0001 0.0002 0.0007 0.0005 0.0008 0.0025 0.0008 0.0003 0.0011
Bud Periphery 0.0012 0.0033 0.0017 0.0036 0.0031 0.0015 0.0011 0.0002 0.0026 0.0003 0.0003 0.0006 0.0005 0.0011 0.0039 0.0028 0.0009 0.0015
Bud Site 0.0014 0.0072 0.0024 0.0187 0.0108 0.0014 0.0104 0.0007 0.0024 0.0003 0.0025 0.0004 0.0008 0.0058 0.0068 0.0012 0.0006 0.0009
Cell Periphery 0.003 0.0004 0.0003 0.0009 0.0007 0.0004 0.0012 0.0004 0.0005 0.0001 0.0002 0.0003 0.0089 0.0201 0.0082 0.0194 0.0003 0.0144
Cytoplasm 0.0219 0.1375 0.1261 0.0637 0.0691 0.0686 0.0767 0.0921 0.1335 0.0098 0.1633 0.1213 0.0695 0.0952 0.1029 0.1083 0.0449 0.0728
Cytoplasmic Foci 0.0226 0.0262 0.042 0.0403 0.026 0.0312 0.0436 0.055 0.0272 0.03 0.0384 0.0226 0.0302 0.0192 0.0324 0.0362 0.0158 0.0241
Eisosomes 0.0003 0 0 0.0002 0.0001 0 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0065 0.0066 0.0094 0.0016 0.004 0.005 0.0211 0.0089 0.0102 0.0016 0.0031 0.0083 0.0099 0.0077 0.0129 0.0023 0.0061 0.0058
Endosome 0.3395 0.2494 0.3378 0.3444 0.2845 0.3237 0.268 0.3404 0.3872 0.3199 0.4952 0.2663 0.3782 0.2902 0.3631 0.3602 0.4001 0.3071
Golgi 0.0078 0.0021 0.0063 0.0153 0.022 0.0062 0.0115 0.0024 0.0067 0.0043 0.0062 0.0055 0.0045 0.0029 0.0072 0.0117 0.0044 0.0036
Lipid Particles 0.0089 0.0021 0.0077 0.0106 0.027 0.0052 0.0166 0.0076 0.0037 0.0068 0.0018 0.0049 0.0058 0.0045 0.0025 0.0008 0.0029 0.0033
Mitochondria 0.0181 0.004 0.012 0.046 0.0586 0.0048 0.0085 0.0051 0.0097 0.003 0.0018 0.0048 0.0058 0.0027 0.0203 0.0056 0.0016 0.0038
None 0.0105 0.0988 0.0525 0.0084 0.1057 0.011 0.014 0.1217 0.0155 0.0008 0.0095 0.1157 0.0323 0.0336 0.0238 0.0105 0.0216 0.0572
Nuclear Periphery 0.027 0.012 0.0188 0.0034 0.016 0.0121 0.0608 0.0142 0.0182 0.0053 0.0039 0.0122 0.0099 0.018 0.0183 0.004 0.0083 0.0132
Nucleolus 0.0053 0.0029 0.0028 0.0049 0.0009 0.0045 0.0031 0.0017 0.0007 0.0003 0.0009 0.0008 0.0016 0.0079 0.0016 0.0005 0.0005 0.0009
Nucleus 0.0356 0.011 0.0173 0.0072 0.0027 0.02 0.0549 0.0054 0.0053 0.0012 0.0039 0.0053 0.0079 0.0105 0.0059 0.0033 0.0017 0.005
Peroxisomes 0.0043 0.0004 0.0145 0.0076 0.0059 0.0008 0.0297 0.0006 0.0005 0.1286 0.0005 0.0022 0.0004 0.0004 0.0011 0.0005 0.0005 0.0005
Punctate Nuclear 0.0033 0.0011 0.0046 0.0022 0.0019 0.0015 0.0018 0.0013 0.0004 0.0005 0.001 0.0005 0.0009 0.0006 0.0007 0.0002 0.0002 0.0004
Vacuole 0.4033 0.3691 0.2798 0.3207 0.2603 0.405 0.3218 0.2935 0.3038 0.3279 0.2332 0.3088 0.3546 0.4001 0.3059 0.3506 0.3882 0.3787
Vacuole Periphery 0.0763 0.0607 0.0595 0.0776 0.0951 0.0927 0.036 0.0484 0.07 0.1503 0.0335 0.1018 0.0719 0.0768 0.0776 0.0788 0.1003 0.1048

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.527 11.2821 10.3651 16.0359 19.48 6.0065 22.4072 24.7473 20.86 24.0273
Translational Efficiency 0.271 0.4904 0.4438 0.2362 0.2489 0.9277 0.2934 0.2201 0.2193 0.2784

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene
Localization
Cell Percentages vacuolar membrane (56%), vacuole (8%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YCR023C

YCR023C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ycr023c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available