ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.09 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0 0.18 0.15 0.09 0 0.08 0 0.05 0.05 0.11
Bud 0 0 0 0 0 0 0.06 0.06 0 0.09 0.09 0.09 0 0 0 0 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.12 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.11 0.07 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.12 0.06 0.05 0
Golgi 0.4 0.56 0.11 0.16 0.42 0.1 0.06 0.05 0 0 0 0 0.39 0.29 0.18 0.84 0.73 0.77 0.81 0.7 0.71 0.76 0.81 0.76
Mitochondria 0.87 0.72 0.95 0.91 0.69 0.87 0.78 0.75 0.87 0.84 0.86 0.84 0.89 0.89 0.82 0.06 0.11 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0.05 0 0.08 0.08 0.1 0.1 0.07 0.06 0 0 0 0 0.05 0.07 0.12 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0.1 0.16 0.15 0.09 0.18 0.1 0.2 0 0 0.08 0 0 0 0 0 0.09 0.05 0 0
SpindlePole 0 0 0 0 0 0 0.05 0 0 0.08 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.08 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0.1 0.11 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 28 19 6 5 17 5 13 21 1 10 11 20 16 9 5 27 16 9 11 17 6 4 13 28
Bud 5 2 6 4 11 17 38 45 12 44 39 47 6 5 5 2 7 0 1 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 0 1 2 9 1 6 2 10 0 0 0 0 0 0
Cell Periphery 1 4 0 0 0 1 1 2 0 1 1 1 0 0 0 2 0 2 0 0 0 0 0 0
Cytoplasm 3 9 5 7 13 7 11 12 4 2 8 8 9 13 18 10 13 7 0 3 0 1 1 2
Endoplasmic Reticulum 6 4 1 2 1 4 0 2 0 0 2 1 5 3 4 2 4 5 0 0 0 1 2 1
Endosome 11 2 0 8 34 27 42 51 5 8 12 7 5 9 12 3 2 3 24 19 18 5 11 11
Golgi 148 113 17 35 128 41 38 42 4 7 7 4 106 95 54 123 78 80 316 156 108 71 201 199
Mitochondria 324 147 144 200 209 359 469 583 247 425 361 443 239 288 245 9 12 10 12 8 3 2 4 5
Nucleus 0 0 1 1 0 0 0 4 0 1 2 1 1 1 0 0 0 0 0 1 0 0 0 1
Nuclear Periphery 3 3 2 1 0 2 5 2 4 3 2 1 5 5 2 1 0 0 0 0 0 0 0 0
Nucleolus 25 7 2 12 9 32 50 78 27 33 27 21 6 13 6 8 7 13 0 0 0 0 0 0
Peroxisomes 2 0 0 2 11 40 95 119 26 91 42 105 4 13 24 0 0 0 15 4 13 4 6 6
SpindlePole 3 0 1 2 3 9 27 34 8 42 18 26 0 0 0 4 2 1 0 1 0 0 1 0
Vac/Vac Membrane 32 17 8 1 4 7 10 10 3 3 2 1 16 8 4 15 12 15 5 4 0 1 3 2
Unique Cell Count 374 203 152 220 304 413 598 773 283 504 420 529 269 324 299 146 107 104 390 223 154 94 249 262
Labelled Cell Count 592 327 193 280 440 552 801 1015 342 676 536 696 418 462 379 206 153 145 390 223 154 94 249 262


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.1 16.9 9.1 11.9 12.4 10.8 9.9 10.0 8.8 9.1 8.9 8.8 14.6 14.3 13.5 22.8 21.3 20.1 14.2 16.1 16.3
Std Deviation (1e-4) 2.8 3.8 1.8 2.0 2.2 1.7 1.7 1.6 1.7 1.6 2.0 3.4 2.5 2.5 2.7 5.5 5.9 6.2 2.6 2.9 3.1
Intensity Change (Log2) 0.39 0.45 0.26 0.13 0.14 -0.05 0.0 -0.03 -0.04 0.69 0.66 0.57 1.33 1.23 1.15 0.65 0.83 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0.8 0 0 0 0 0 0 -0.1 0.9 0 0 4.0 3.1 1.6
Bud 0 -0.2 0.1 1.1 0.9 0.1 1.9 2.1 2.0 0 0 0 0 0.9 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.5 0 0 0 0 0 0 0 0 0.4 1.2 1.4 2.8 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 4.3 3.2 3.4 3.3 0 0 0 0 0 0 0 0 0 0
Golgi 1.3 6.7 -0.4 -2.0 -2.7 -4.5 -5.6 -5.0 0 6.1 4.3 1.9 12.6 10.1 10.6
Mitochondria -1.4 -6.3 -2.6 -4.6 -5.3 -2.5 -3.3 -2.9 -3.5 -2.0 -2.1 -3.7 -15.3 -13.5 -13.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.1 0 2.9 3.1 3.5 3.3 2.5 2.5 1.6 0 0 0 0 0 3.7
Peroxisomes 0 0 4.0 5.3 5.2 3.9 5.6 4.1 6.0 0 0 3.6 0 0 0
SpindlePole 0 0 0 2.2 2.2 0 3.4 2.1 2.4 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0.3 -1.6 0 1.6 1.8 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.2196 9.8212 9.1926 9.6474 8.7297 9.1 8.5097 10.9901 10.391 10.7265 9.7846 9.8393 10.762 13.464 12.9707 13.0244 11.8627 12.809
Actin 0.0541 0.0174 0.0158 0.0154 0.0033 0.0471 0.0566 0.0129 0.0238 0.0169 0.0231 0.0164 0.0558 0.0171 0.0254 0.0188 0.0039 0.0033
Bud 0.0003 0 0.0003 0.0014 0 0.0001 0.0002 0 0 0.0001 0 0 0.0002 0 0.0002 0 0.0001 0
Bud Neck 0.0005 0.0001 0.0003 0.0006 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0001 0.0003 0.0041 0.0001 0.0003 0.0001 0.0018 0.0001
Bud Periphery 0.0003 0 0.0004 0.0034 0 0.0001 0.0002 0 0 0.0002 0 0.0001 0.0003 0 0.0004 0.0001 0.0001 0
Bud Site 0.0014 0.0001 0.0017 0.0039 0.0001 0.0002 0.0007 0 0.0001 0.0003 0.0001 0.0001 0.0045 0.0001 0.0003 0.0001 0.0009 0
Cell Periphery 0.0002 0 0.0001 0.0004 0 0 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.0007 0.0004 0.0011 0.0004 0.0002 0.0002 0.0048 0.0002 0.0003 0.0005 0.0002 0.0003 0.0029 0.0004 0.0007 0.0003 0.0003 0.0001
Cytoplasmic Foci 0.0495 0.0507 0.04 0.0427 0.0544 0.0384 0.0548 0.0513 0.043 0.048 0.0435 0.0341 0.0614 0.0594 0.0464 0.0448 0.0374 0.0277
Eisosomes 0.0002 0 0.0001 0.0002 0 0.0001 0.0005 0 0 0.0001 0 0 0.0002 0 0.0002 0.0001 0.0002 0
Endoplasmic Reticulum 0.0007 0.0001 0.0007 0.0011 0.0004 0.0001 0.0016 0.0001 0.0007 0.0013 0.0001 0.0003 0.0022 0.0001 0.0005 0.0003 0.0002 0.0001
Endosome 0.0889 0.0743 0.0849 0.083 0.0717 0.0632 0.0659 0.0698 0.0598 0.0774 0.0619 0.0532 0.0914 0.0851 0.0886 0.174 0.0798 0.0663
Golgi 0.6435 0.7965 0.7573 0.7863 0.7066 0.704 0.6631 0.824 0.8185 0.7997 0.7733 0.8476 0.642 0.774 0.7834 0.6672 0.7786 0.866
Lipid Particles 0.051 0.0128 0.0169 0.0147 0.1163 0.0534 0.0454 0.0141 0.0138 0.0365 0.0529 0.0176 0.0489 0.0438 0.021 0.0477 0.0593 0.0159
Mitochondria 0.0442 0.0264 0.0443 0.0076 0.0081 0.049 0.0411 0.0111 0.0119 0.0081 0.0219 0.0095 0.0121 0.0047 0.0134 0.0034 0.0054 0.0062
None 0.0003 0 0.0004 0.0004 0.0002 0.0001 0.002 0 0.0001 0.0007 0 0.0001 0.0033 0 0.0013 0.0006 0.0008 0
Nuclear Periphery 0.0019 0.0001 0.0009 0.0014 0.007 0.0001 0.0022 0 0.0003 0.0014 0.0001 0.0011 0.0054 0.0001 0.0013 0.0003 0.0001 0.0001
Nucleolus 0.0005 0 0.0002 0.0002 0.0001 0 0.0005 0 0 0.0001 0 0.0002 0.0004 0 0.0007 0 0.0007 0
Nucleus 0.0003 0 0.0003 0.0004 0.0001 0 0.0004 0 0 0.0002 0 0.0004 0.0012 0 0.0006 0 0.0002 0
Peroxisomes 0.046 0.0177 0.0252 0.032 0.0269 0.041 0.055 0.0156 0.0262 0.0057 0.021 0.0136 0.051 0.0095 0.01 0.0333 0.0237 0.0104
Punctate Nuclear 0.004 0 0.0015 0.0003 0.0014 0.0004 0.002 0 0.0001 0.0008 0.0001 0.0019 0.0074 0.0001 0.0024 0.0007 0.0029 0
Vacuole 0.0053 0.0005 0.0021 0.0023 0.0003 0.0004 0.001 0.0002 0.0003 0.0005 0.0003 0.0008 0.0011 0.0018 0.0008 0.0026 0.001 0.0008
Vacuole Periphery 0.0061 0.0028 0.0055 0.0017 0.0027 0.0019 0.0015 0.0006 0.0008 0.0011 0.0012 0.0021 0.004 0.0034 0.0019 0.0054 0.0026 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 60.3424 48.8051 47.6519 57.9556 57.7461 74.6683 85.0582 60.4762 49.4109 64.4676
Translational Efficiency 1.5409 2.2336 1.807 1.3422 1.4168 1.1351 1.088 1.1113 1.0007 1.1797

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1573 1456 224 493 453 1493 237 1321 2026 2949 461 1814

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1141.25 1315.26 1537.59 1532.57 1231.97 1268.37 1639.02 1443.02 1161.53 1291.52 1589.74 1467.36
Standard Deviation 199.95 223.70 190.31 256.17 173.54 202.30 184.85 223.06 198.00 214.42 194.25 235.92
Intensity Change Log 2 0.204733 0.430056 0.425338 0.042009 0.411866 0.228124 0.122554 0.420642 0.326330

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.029763 0.039332 0.058236 0.036875 0.031090 0.022274 0.032483 0.041370 0.030060 0.030696 0.044997 0.040149
Bud Neck 0.000867 0.000464 0.002752 0.001816 0.002084 0.000354 0.001882 0.000880 0.001139 0.000409 0.002305 0.001134
Bud Site 0.000545 0.000535 0.001349 0.002454 0.001547 0.000456 0.001035 0.002188 0.000769 0.000495 0.001187 0.002260
Cell Periphery 0.000072 0.000068 0.000186 0.000288 0.000758 0.000062 0.000154 0.000183 0.000225 0.000065 0.000170 0.000212
Cytoplasm 0.000658 0.000608 0.000313 0.000472 0.000564 0.000534 0.000256 0.000849 0.000637 0.000570 0.000284 0.000747
Cytoplasmic Foci 0.135833 0.141969 0.098013 0.081764 0.178316 0.183763* 0.074285 0.090810 0.145332 0.163128 0.085814 0.088351
Eisosomes 0.000533 0.000436 0.000389 0.000299 0.000323 0.000251 0.000268 0.000361 0.000486 0.000343 0.000327 0.000344
Endoplasmic Reticulum 0.000375 0.000417 0.001433 0.001674 0.000655 0.000299 0.001523 0.001067 0.000438 0.000357 0.001479 0.001232
Endosome 0.048257 0.060360 0.067330 0.094865 0.079500 0.075844 0.072870 0.103049 0.055242 0.068199 0.070178 0.100825
Golgi 0.625623* 0.630184* 0.618173* 0.637748* 0.593475* 0.610043* 0.704719* 0.642384* 0.618435* 0.619987* 0.662666* 0.641124*
Lipid Particles 0.104987* 0.091513 0.114722* 0.064420 0.064058 0.053441 0.068221 0.063130 0.095835 0.072238 0.090816 0.063480
Mitochondria 0.033937 0.019628 0.012764 0.021010 0.026693 0.036577 0.009604 0.021875 0.032317 0.028209 0.011139 0.021640
Mitotic Spindle 0.000331 0.000990 0.003769 0.004162 0.001714 0.000698 0.001684 0.001320 0.000640 0.000842 0.002697 0.002093
None 0.000133 0.000105 0.000139 0.000140 0.000082 0.000078 0.000133 0.000109 0.000122 0.000091 0.000136 0.000118
Nuclear Periphery 0.000085 0.000205 0.000904 0.000644 0.000136 0.000144 0.001607 0.000384 0.000096 0.000175 0.001266 0.000454
Nuclear Periphery Foci 0.000227 0.000185 0.000950 0.000960 0.000240 0.000170 0.001158 0.001032 0.000230 0.000177 0.001057 0.001012
Nucleolus 0.000048 0.000018 0.000020 0.000034 0.000019 0.000012 0.000023 0.000050 0.000041 0.000015 0.000021 0.000045
Nucleus 0.000031 0.000018 0.000035 0.000027 0.000021 0.000015 0.000024 0.000022 0.000029 0.000016 0.000030 0.000024
Peroxisomes 0.014193 0.007762 0.001976 0.003794 0.005804 0.008302 0.002362 0.002612 0.012318 0.008035 0.002175 0.002933
Vacuole 0.001618 0.001974 0.008804 0.027090 0.006958 0.002878 0.013847 0.015260 0.002812 0.002432 0.011396 0.018475
Vacuole Periphery 0.001887 0.003229 0.007744 0.019464 0.005965 0.003806 0.011862 0.011065 0.002798 0.003521 0.009861 0.013348

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.51 -2.89 -1.24 0.38 1.94 1.76 -0.18 -1.75 -4.57 -1.27 -0.23 -2.49 -2.74 -2.68 0.75
Bud Neck 1.61 -2.46 -2.15 -3.77 1.15 2.08 0.22 1.42 -3.49 2.58 2.72 -2.42 0.02 -4.94 2.78
Bud Site 0.11 -2.37 -4.64 -4.79 -2.35 2.36 1.04 -0.99 -3.23 -2.09 1.63 -1.72 -3.46 -4.31 -2.52
Cell Periphery 0.43 -2.18 -2.53 -2.59 -1.04 1.10 0.96 0.91 -4.71 -0.86 1.14 0.39 0.09 -4.93 -1.06
Cytoplasm 0.69 5.73 2.55 1.94 -3.03 0.33 3.29 -1.19 -1.39 -2.54 1.25 7.01 -0.68 -1.08 -2.71
Cytoplasmic Foci -1.35 5.14 9.31 10.16 1.97 -0.75 12.64 12.67 19.60 -2.70 -4.70 11.60 15.05 20.92 -0.50
Eisosomes 2.03 2.17 4.35 2.91 1.37 1.52 1.01 -0.70 -3.49 -2.25 4.09 3.63 3.62 -0.06 -0.44
Endoplasmic Reticulum -0.50 -7.80 -3.59 -3.41 -0.66 2.74 -2.17 -2.32 -5.93 1.13 1.46 -4.99 -5.70 -6.21 0.98
Endosome -3.68 -2.95 -8.22 -5.95 -3.43 0.69 0.83 -4.08 -6.21 -4.10 -4.82 -3.07 -12.90 -9.48 -5.76
Golgi -0.51 0.40 -0.79 -0.46 -0.87 -1.31 -5.75 -3.60 -3.23 3.55 -0.23 -3.37 -2.59 -2.57 1.61
Lipid Particles 2.22 -0.77 5.12 3.47 3.72 1.56 -0.40 0.12 -1.89 0.53 5.22 0.62 6.27 1.98 3.36
Mitochondria 4.60 5.41 2.97 -0.34 -1.75 -1.79 3.12 0.93 3.90 -3.31 1.46 7.00 3.65 2.50 -3.67
Mitotic Spindle -0.99 -1.06 -1.90 -1.51 -0.10 0.69 -0.00 0.24 -1.75 0.29 -0.42 -1.21 -2.15 -1.87 0.33
None 1.45 -0.28 -0.40 -1.46 -0.08 0.45 -2.68 -1.25 -1.51 0.86 2.76 -1.02 0.22 -1.48 0.89
Nuclear Periphery -1.39 -4.22 -5.87 -3.41 1.21 -0.31 -1.88 -4.62 -4.59 1.56 -1.78 -2.83 -8.00 -4.51 1.95
Nuclear Periphery Foci 0.51 -2.20 -3.77 -4.00 -0.02 0.93 -1.71 -2.38 -2.63 0.18 0.88 -2.58 -3.14 -3.40 0.09
Nucleolus 2.71 2.60 1.11 -2.31 -2.16 1.32 -0.50 -1.06 -1.30 -0.92 3.14 2.26 -0.20 -1.44 -1.14
Nucleus 1.41 -0.31 0.43 -1.65 1.03 1.93 -0.88 -0.52 -2.28 0.37 1.78 -0.05 0.70 -2.33 1.30
Peroxisomes 3.77 8.04 5.74 2.89 -1.61 -1.88 2.69 3.43 5.19 -0.25 3.20 7.97 7.66 6.50 -1.16
Vacuole -0.99 -4.88 -7.28 -7.18 -4.86 2.79 -2.62 -3.88 -7.48 -0.53 0.92 -6.15 -10.04 -10.47 -3.52
Vacuole Periphery -2.57 -4.96 -8.25 -7.44 -4.83 1.71 -3.12 -3.51 -7.71 0.47 -1.64 -7.09 -11.78 -10.84 -2.74
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene
Localization
Cell Percentages Golgi (11%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YCR043C

YCR043C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ycr043c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available