Standard name
Human Ortholog
Description Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.96 0.95 0.88 0.8 0.75 – 0.69 0.73 0.66 0.67 1.0 0.98 0.98 1.0 0.94 0.92 0.97 0.94 0.95 0.93 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.11 0.08 0.21 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0.05 0 0.07 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.07 0.15 0.23 0.24 – 0.24 0.19 0.22 0.19 0 0 0 0 0.07 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 1 0 0 0 0 0 – 1 0 2 6 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 5 0 6 – 1 11 5 9 0 0 0 2 1 3 0 0 0 0 0 0
Cytoplasm 150 206 327 208 273 242 403 – 229 301 282 373 289 364 418 248 197 281 147 194 319 97 279 365
Endoplasmic Reticulum 0 0 0 0 2 1 0 – 1 6 2 12 0 0 1 11 7 12 0 0 1 0 1 3
Endosome 0 0 0 0 0 2 1 – 0 1 1 2 1 0 0 0 1 0 0 2 0 0 0 0
Golgi 0 0 2 0 1 0 0 – 0 0 0 0 0 0 0 0 1 0 0 1 3 0 0 0
Mitochondria 0 1 0 1 1 6 11 – 38 33 89 79 0 0 0 2 3 1 0 1 1 0 0 1
Nucleus 5 2 5 2 6 3 14 – 14 22 16 39 2 2 4 7 12 9 0 2 3 2 3 2
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 3 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 18 16 46 69 131 – 81 78 96 108 1 8 14 0 14 34 4 2 1 1 1 3
Unique Cell Count 153 207 340 219 309 301 535 331 412 430 558 290 370 425 249 209 305 153 208 336 105 293 383
Labelled Cell Count 155 211 353 227 334 323 567 366 452 493 633 293 374 438 270 236 340 153 208 336 105 293 383


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.5 9.1 11.0 10.5 10.6 9.0 8.4 – 7.6 7.9 7.4 7.5 11.0 10.6 10.3 8.4 10.2 10.7 11.4 11.3 11.5
Std Deviation (1e-4) 1.6 1.9 1.6 1.6 1.5 1.4 1.2 – 1.2 1.2 1.2 1.2 1.6 1.5 1.5 1.3 1.8 2.3 1.5 1.8 1.7
Intensity Change (Log2) – – – -0.07 -0.06 -0.3 -0.39 – -0.54 -0.47 -0.56 -0.55 -0.0 -0.06 -0.1 -0.39 -0.12 -0.04 0.05 0.04 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 2.6 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -3.8 -6.3 -8.1 – -9.3 -8.5 -10.4 -10.3 3.0 1.8 1.9 2.7 -1.0 -2.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 3.7
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 6.4 5.3 8.9 7.3 0 0 0 0 0 0
Nucleus 0 0.5 0 1.1 – 2.2 2.8 1.9 3.7 0 0 0 1.1 2.8 1.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 4.1 6.5 7.4 – 7.0 5.6 6.6 5.9 -3.6 -2.2 -1.4 -3.7 0.7 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0011 5.1304 4.6823 3.7268 4.449 4.4724 6.4069 8.2098 7.1521 7.5925 7.43 7.3028 3.0859 4.4828 4.3847 3.9333 4.4472 3.9128
Actin 0.0193 0.0009 0.0151 0.0122 0.0011 0.0015 0.3553 0.0131 0.0084 0.0013 0.006 0.0369 0.0231 0.0002 0.0051 0.0008 0.0002 0.0205
Bud 0.0006 0.0003 0.0009 0.0024 0.0001 0.0016 0.0019 0.0003 0.0003 0.0002 0.0057 0.0012 0.0009 0.0001 0.0003 0.0001 0.0002 0.0001
Bud Neck 0.0014 0.0002 0.0016 0.0058 0.0005 0.0011 0.0011 0.0002 0.0092 0.0006 0.0021 0.0051 0.0075 0.0001 0.0011 0.0004 0.0004 0.0009
Bud Periphery 0.0005 0.0001 0.0007 0.0015 0 0.0007 0.0026 0.0002 0.0003 0 0.0034 0.0016 0.0012 0 0.0003 0 0 0.0001
Bud Site 0.0016 0.001 0.0021 0.0071 0.0002 0.0002 0.0183 0.0006 0.0171 0.0002 0.0223 0.0072 0.0157 0.0002 0.0054 0.0002 0.0001 0.0001
Cell Periphery 0.0001 0 0.0001 0.0005 0 0 0.0003 0.0001 0.0005 0 0.0004 0.0009 0.0008 0 0.0002 0 0 0.0001
Cytoplasm 0.8252 0.9654 0.9157 0.8222 0.9008 0.9016 0.3283 0.9215 0.925 0.9633 0.8136 0.9117 0.8175 0.9845 0.9403 0.9577 0.9046 0.9442
Cytoplasmic Foci 0.0203 0.0064 0.0069 0.0202 0.0306 0.0091 0.0355 0.0043 0.0016 0.0033 0.0111 0.005 0.0202 0.0012 0.0034 0.0038 0.0029 0.0029
Eisosomes 0.0001 0 0 0.0003 0 0 0.0014 0.0001 0.0001 0 0 0.0002 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0078 0.0014 0.0031 0.001 0.0013 0.0036 0.0013 0.0014 0.0038 0.0022 0.0009 0.0017 0.0028 0.0017 0.0021 0.0041 0.0018 0.0101
Endosome 0.0231 0.0017 0.0046 0.0049 0.0075 0.0085 0.0269 0.0156 0.001 0.0011 0.0052 0.0022 0.012 0.0012 0.0088 0.0031 0.0027 0.0031
Golgi 0.0047 0.0004 0.0029 0.0062 0.0053 0.0007 0.0304 0.0098 0.0004 0.0001 0.0056 0.0044 0.0112 0 0.0036 0.0001 0.0001 0.0007
Lipid Particles 0.0062 0 0.0011 0.0137 0.0029 0.0003 0.0774 0.0007 0.0002 0 0.0002 0.0009 0.0152 0 0.0048 0 0 0.0005
Mitochondria 0.003 0.0003 0.0088 0.0423 0.0009 0.0056 0.0282 0.002 0.0002 0.0002 0.0068 0.0023 0.0121 0.0001 0.0017 0.0001 0.0004 0.0002
None 0.0219 0.0091 0.0111 0.0081 0.0043 0.0286 0.0081 0.0245 0.0048 0.0073 0.0023 0.0032 0.0073 0.0042 0.0079 0.0156 0.0047 0.0036
Nuclear Periphery 0.0207 0.0011 0.0022 0.0016 0.0015 0.0031 0.0023 0.0015 0.0023 0.0022 0.0225 0.0008 0.0031 0.0009 0.0022 0.0026 0.0064 0.0019
Nucleolus 0.0007 0 0.0001 0.0098 0 0.0004 0.0012 0 0.0001 0.0001 0.0005 0.0001 0.0011 0 0.0001 0 0.0001 0
Nucleus 0.0229 0.0077 0.0116 0.0139 0.0101 0.0283 0.0011 0.0022 0.0235 0.0142 0.0853 0.011 0.0239 0.0045 0.0066 0.0093 0.0733 0.0092
Peroxisomes 0.0091 0.0016 0.0078 0.0221 0.0305 0.001 0.0688 0.0007 0.0001 0.0001 0.003 0.0006 0.0105 0 0.0037 0.0001 0 0.0002
Punctate Nuclear 0.0065 0.0018 0.0022 0.0013 0.0017 0.0026 0.0074 0.0007 0.0005 0.0031 0.0014 0.0007 0.0079 0.0002 0.0009 0.001 0.0009 0.0006
Vacuole 0.003 0.0005 0.001 0.0019 0.0005 0.0012 0.0013 0.0005 0.0005 0.0003 0.001 0.0021 0.0043 0.0007 0.0008 0.0008 0.0008 0.0006
Vacuole Periphery 0.0012 0.0001 0.0004 0.0014 0.0001 0.0003 0.001 0.0004 0.0001 0.0001 0.0007 0.0001 0.0016 0.0001 0.0004 0.0002 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 71.0041 55.8566 47.1 39.5504 58.9184 56.7719 54.2303 48.5136 47.7081 56.171
Translational Efficiency 0.5782 0.6214 0.6693 0.8659 0.5982 0.6764 0.6826 0.6932 0.7299 0.5731

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Yih1

Yih1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yih1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available