Standard name
Human Ortholog
Description Forkhead transcription factor; drives S-phase activation of genes involved in chromosome segregation, spindle dynamics, budding; also activates genes involved in respiration, use of alternative energy sources (like proline), NAD synthesis, oxidative stress resistance; key factor in early adaptation to nutrient deficiency and diauxic shift; suppressor of calmodulin mutants with specific SPB assembly defects; ortholog of C. elegans lifespan regulator PHA-4

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.07 0 0.05 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.71 0.73 0.73 0.74 0.52 0.59 0.59 0.54 0.52 0.45 0.52 0.5 0.53 0.85 0.81 0.79 0.56 0.55 0.55 0.68 0.73 0.56
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.07 0.2 0.19 0.43 0.22 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.2 0.16 0.18 0.21 0.38 0.29 0.26 0.23 0.27 0.21 0.27 0.45 0.31 0.08 0.07 0.1 0.22 0.2 0.18 0.08 0.05 0.13
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0.06
Vac/Vac Membrane 0.07 0.14 0.1 0.06 0.12 0.11 0.13 0.13 0.08 0.12 0.1 0.19 0.17 0.07 0.12 0.14 0 0 0.05 0.06 0.1 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 0 1 0 0 2 0 3 2 6 1 0 1 0 0 0 20 12 16 0 1 2
Bud Neck 0 0 0 1 0 1 0 3 6 0 1 0 0 0 0 0 4 2 4 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0
Cell Periphery 0 2 1 2 2 1 1 1 2 0 1 0 0 5 2 2 0 0 0 0 0 0
Cytoplasm 223 121 146 225 177 223 268 158 203 120 214 29 43 112 136 170 182 94 122 10 18 28
Endoplasmic Reticulum 0 1 0 3 0 1 0 1 0 1 3 1 0 13 11 12 0 0 1 0 0 0
Endosome 3 1 3 1 5 4 11 2 5 0 2 0 2 0 4 0 6 1 2 0 0 0
Golgi 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0
Mitochondria 12 1 0 2 4 17 31 58 73 113 90 0 0 1 0 6 2 5 7 0 0 2
Nucleus 63 26 35 65 130 108 117 66 104 55 111 26 25 11 12 21 70 35 38 1 1 6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 3 0 0 0 0 2 6 0 2 9 1 0 0 0 0 0 6 2 4 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 1 0 0 0 1 1 1 0 6 1 0 0 0 0 19 8 7 0 0 3
Vac/Vac Membrane 23 23 19 18 40 42 57 39 33 31 41 11 14 9 21 30 6 5 10 1 2 2
Unique Cell Count 312 166 199 306 342 375 452 293 389 264 413 58 81 131 168 214 325 172 222 16 25 51
Labelled Cell Count 329 175 208 319 359 401 493 332 432 337 472 68 85 151 186 242 325 172 222 16 25 51


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.9 6.3 5.9 6.9 5.7 5.8 5.0 5.0 4.4 4.9 7.1 7.0 7.0 7.2 7.0
Std Deviation (1e-4) 0.9 1.5 1.2 1.3 1.4 1.2 1.2 1.4 1.4 1.0 1.3 1.5 1.7 1.2 1.6 1.6
Intensity Change (Log2) -0.09 0.14 -0.13 -0.11 -0.32 -0.33 -0.5 -0.37 0.18 0.16 0.16 0.21 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -4.9 -3.3 -3.4 -4.4 -4.9 -6.0 -5.1 -3.4 -3.3 2.6 1.7 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 4.5 3.7 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.0 3.8 6.7 6.5 0 0 0 0 0 0 0
Nucleus 1.0 5.0 3.0 2.3 1.3 2.5 0.9 2.5 4.3 2.5 -2.4 -3.0 -2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.5 0.8 0.6 1.1 1.3 -0.4 0.8 0.1 2.0 1.8 -0.9 0.9 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9148 2.327 2.0296 1.1191 1.0873 1.4491 0.6825 2.7245 1.9432 1.2323 1.3372 1.3232 1.0202 2.5891 2.597 1.6195 0.9762 1.4758
Actin 0.0598 0.0004 0.0139 0.0047 0.0058 0.0012 0.0187 0.0021 0.0079 0.0692 0.0081 0.0085 0.048 0.0004 0.0216 0.0158 0.006 0.0041
Bud 0.0078 0.0007 0.0016 0.0121 0.0014 0.0032 0.01 0.0101 0.0091 0.0128 0.0161 0.0049 0.0147 0.0012 0.0018 0.001 0.0026 0.002
Bud Neck 0.0047 0.0008 0.0026 0.002 0.0029 0.0089 0.0059 0.001 0.0024 0.0041 0.0064 0.0071 0.0047 0.0008 0.0017 0.0019 0.0019 0.0073
Bud Periphery 0.0084 0.0004 0.0012 0.0217 0.0014 0.0038 0.0088 0.0039 0.0065 0.0217 0.0114 0.0052 0.015 0.0005 0.0012 0.0009 0.0023 0.0016
Bud Site 0.0249 0.006 0.0079 0.0074 0.0031 0.0009 0.0254 0.018 0.0177 0.0031 0.0025 0.0011 0.0211 0.0034 0.012 0.0025 0.0016 0.0011
Cell Periphery 0.0012 0.0002 0.0002 0.0003 0.0002 0.0002 0.0011 0.0003 0.0006 0.0007 0.0006 0.0002 0.0015 0.0001 0.0003 0.0002 0.0002 0.0001
Cytoplasm 0.2529 0.2417 0.2167 0.5262 0.4585 0.4276 0.2849 0.2803 0.2745 0.4228 0.4478 0.4275 0.3368 0.252 0.2146 0.6264 0.6275 0.5087
Cytoplasmic Foci 0.0206 0.0055 0.0187 0.0313 0.0165 0.0135 0.032 0.0066 0.0114 0.0322 0.0231 0.0076 0.0148 0.0044 0.01 0.03 0.0401 0.0136
Eisosomes 0.0008 0 0.0002 0.0001 0.0001 0 0.0006 0.0001 0.0001 0.0004 0.0002 0 0.0005 0 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0097 0.006 0.0049 0.0043 0.0053 0.0037 0.0134 0.0024 0.0052 0.0055 0.012 0.0023 0.0136 0.0023 0.0027 0.0117 0.0093 0.0036
Endosome 0.0251 0.003 0.0203 0.03 0.0505 0.0052 0.0309 0.0016 0.0117 0.0093 0.0251 0.0053 0.0417 0.0028 0.0072 0.0498 0.0181 0.0188
Golgi 0.0137 0.0001 0.005 0.0108 0.0084 0.0005 0.0058 0.0002 0.0044 0.0032 0.0088 0.0007 0.0109 0.0009 0.0031 0.031 0.0077 0.0009
Lipid Particles 0.0075 0.0004 0.0052 0.0134 0.0156 0.0009 0.0069 0.0002 0.0086 0.0018 0.0117 0.0033 0.0129 0.007 0.0031 0.0268 0.03 0.001
Mitochondria 0.011 0.0004 0.0038 0.0025 0.005 0.0009 0.0087 0.0004 0.0053 0.0073 0.053 0.0009 0.0107 0.0005 0.005 0.0102 0.0018 0.0009
None 0.2383 0.1023 0.0764 0.2168 0.1906 0.1734 0.2169 0.0923 0.0866 0.292 0.2841 0.1747 0.0506 0.0628 0.0464 0.092 0.0745 0.1071
Nuclear Periphery 0.0156 0.0103 0.0134 0.0067 0.02 0.0103 0.0379 0.0041 0.0075 0.005 0.0049 0.0037 0.0176 0.0058 0.0091 0.0054 0.0122 0.0058
Nucleolus 0.0176 0.0073 0.0036 0.0031 0.0047 0.0129 0.0102 0.003 0.0065 0.0038 0.0011 0.0044 0.0121 0.0039 0.0033 0.0004 0.0012 0.0035
Nucleus 0.231 0.5917 0.5788 0.0682 0.1801 0.3189 0.2304 0.5578 0.5061 0.0706 0.0377 0.3279 0.3208 0.6357 0.6371 0.0569 0.0512 0.3056
Peroxisomes 0.0067 0.0001 0.0039 0.0137 0.0033 0.0002 0.0069 0.0011 0.0088 0.022 0.0066 0.002 0.0128 0.0017 0.007 0.024 0.0759 0.0014
Punctate Nuclear 0.0252 0.0197 0.0164 0.0202 0.0161 0.0096 0.0271 0.0118 0.0119 0.0075 0.0057 0.009 0.0106 0.0108 0.0101 0.0065 0.0261 0.0062
Vacuole 0.0151 0.0026 0.004 0.0037 0.0085 0.0034 0.0139 0.0025 0.0066 0.0041 0.0206 0.0033 0.0249 0.0027 0.002 0.0048 0.0081 0.0058
Vacuole Periphery 0.0024 0.0004 0.0012 0.0008 0.0022 0.0007 0.0036 0.0002 0.0008 0.0009 0.0125 0.0003 0.0037 0.0004 0.0006 0.0017 0.0017 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.2859 25.0231 17.2728 33.1022 76.8057 59.8566 29.4196 19.2605 29.5397 71.0872
Translational Efficiency 0.8728 0.7422 0.3466 0.5621 0.9122 1.175 0.6381 0.5637 0.5251 0.9789

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
344 1368 1845 235 1799 1923 236 1303 2143 3291 2081 1538

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 632.66 777.34 961.50 887.50 727.92 734.56 1042.18 859.12 712.63 752.34 970.65 863.46
Standard Deviation 127.55 119.15 164.77 107.12 88.44 105.10 122.23 133.40 101.99 113.14 162.54 130.13
Intensity Change Log 2 0.297115 0.603856 0.488317 0.013100 0.517753 0.239080 0.152141 0.558430 0.360344

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000265 0.000517 0.000723 0.000911 0.000111 0.000470 0.000582 0.000523 0.000136 0.000489 0.000707 0.000583
Bud Neck 0.008055 0.015091 0.011695 0.004805 0.006603 0.026252 0.003418 0.005547 0.006836 0.021613 0.010757 0.005433
Bud Site 0.004086 0.007792 0.007966 0.004648 0.002747 0.013862 0.007592 0.005400 0.002962 0.011339 0.007924 0.005285
Cell Periphery 0.000821 0.000393 0.000291 0.000417 0.000255 0.000356 0.000257 0.000221 0.000346 0.000372 0.000287 0.000251
Cytoplasm 0.208591* 0.374689* 0.293867* 0.334565* 0.464946* 0.381799* 0.335069* 0.450568* 0.423795* 0.378844* 0.298540* 0.432843*
Cytoplasmic Foci 0.047175 0.078568 0.004120 0.025650 0.082798 0.091258 0.029408 0.012147 0.077080 0.085983 0.006988 0.014210
Eisosomes 0.000089 0.000062 0.000029 0.000097 0.000058 0.000063 0.000116 0.000031 0.000063 0.000063 0.000039 0.000041
Endoplasmic Reticulum 0.002321 0.002494 0.003951 0.009704 0.000995 0.001693 0.006767 0.002745 0.001208 0.002026 0.004271 0.003808
Endosome 0.002214 0.008166 0.001481 0.005496 0.004553 0.009761 0.006348 0.002314 0.004178 0.009098 0.002033 0.002800
Golgi 0.000196 0.002311 0.000166 0.000194 0.001393 0.003463 0.000126 0.000165 0.001201 0.002985 0.000162 0.000170
Lipid Particles 0.004917 0.003539 0.000750 0.003368 0.003679 0.004849 0.002989 0.001063 0.003878 0.004304 0.001004 0.001415
Mitochondria 0.001147 0.007267 0.001202 0.000473 0.001651 0.008394 0.000402 0.000716 0.001570 0.007926 0.001111 0.000679
Mitotic Spindle 0.001377 0.013349 0.017398 0.002064 0.002565 0.011806 0.001659 0.017763 0.002374 0.012447 0.015613 0.015365
None 0.028921 0.018085 0.010792 0.007342 0.013083 0.017996 0.003149 0.015659 0.015625 0.018033 0.009925 0.014389
Nuclear Periphery 0.001569 0.000748 0.001513 0.001570 0.000737 0.000628 0.001714 0.001050 0.000871 0.000678 0.001536 0.001129
Nuclear Periphery Foci 0.000680 0.000969 0.001536 0.006379 0.000664 0.000700 0.003446 0.001732 0.000667 0.000812 0.001752 0.002442
Nucleolus 0.019171 0.008626 0.001646 0.003500 0.003268 0.008071 0.000822 0.001615 0.005821 0.008301 0.001552 0.001903
Nucleus 0.660266* 0.423408* 0.613570* 0.501718* 0.389179* 0.381173* 0.430570* 0.440964* 0.432695* 0.398729* 0.592817* 0.450247*
Peroxisomes 0.000990 0.004625 0.000688 0.000489 0.001451 0.006209 0.000865 0.000245 0.001377 0.005551 0.000708 0.000282
Vacuole 0.006593 0.027957 0.026329 0.086351 0.018760 0.029246 0.164469 0.039285 0.016807 0.028710 0.041995 0.046476
Vacuole Periphery 0.000555 0.001343 0.000287 0.000259 0.000502 0.001950 0.000231 0.000247 0.000511 0.001698 0.000281 0.000249

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.44 -6.81 -6.60 -4.46 -1.70 -6.33 -7.94 -9.48 -2.60 2.19 -9.53 -9.36 -11.29 -3.89 1.75
Bud Neck -4.54 -6.71 0.85 5.28 7.51 -12.33 3.51 -1.29 10.74 -4.00 -13.34 -9.87 -0.98 11.37 7.84
Bud Site -3.74 -3.61 -2.05 1.35 1.48 -10.54 -1.46 -5.13 6.34 -0.05 -11.85 -6.24 -5.49 5.97 1.81
Cell Periphery 3.01 3.99 2.97 -0.18 -3.03 -2.73 -0.27 2.16 5.18 3.06 -0.51 2.39 3.66 5.03 2.42
Cytoplasm -10.01 -1.61 -3.43 4.18 -2.80 8.13 11.15 6.67 -0.78 -6.69 5.49 21.12 4.86 0.20 -14.13
Cytoplasmic Foci -3.98 7.65 3.98 11.20 -6.73 -1.73 15.28 21.51 21.55 4.08 -2.14 24.45 21.47 25.93 -7.57
Eisosomes 2.15 4.58 -1.66 -5.38 -8.70 -1.32 -8.30 5.64 7.55 10.69 -0.09 4.56 3.40 4.40 -1.09
Endoplasmic Reticulum -1.14 -8.60 -7.93 -7.75 -5.06 -5.00 -9.75 -12.37 -8.19 6.58 -6.95 -19.43 -13.89 -10.06 3.48
Endosome -7.52 2.32 -4.63 2.59 -6.28 -5.34 -1.85 4.62 8.85 6.34 -6.89 5.00 3.33 10.68 -3.29
Golgi -4.08 0.95 -0.66 4.02 -2.19 -2.58 2.62 2.56 5.24 -1.29 -2.97 2.58 2.55 6.59 -0.63
Lipid Particles 1.30 4.30 1.43 0.20 -6.08 -1.36 1.49 7.60 5.17 7.45 -0.67 8.74 7.35 6.05 -2.46
Mitochondria -4.35 -2.12 4.67 4.82 12.29 -5.99 2.31 1.90 7.43 -2.40 -6.90 0.54 2.14 8.65 9.06
Mitotic Spindle -5.44 -8.47 -1.34 4.81 7.79 -5.27 0.15 -6.16 -2.40 -6.19 -7.00 -7.80 -6.22 -1.64 0.57
None 2.46 4.53 4.94 6.12 1.91 -3.11 9.77 0.58 3.32 -7.36 -1.49 6.56 2.93 4.72 -2.85
Nuclear Periphery 2.47 -1.58 -0.56 -7.30 2.15 1.73 -10.96 -5.33 -6.07 3.68 2.56 -12.26 -5.00 -7.01 4.12
Nuclear Periphery Foci -1.66 -4.24 -5.13 -4.90 -4.29 -0.96 -7.86 -4.62 -3.41 4.40 -1.63 -6.63 -6.74 -5.80 -2.28
Nucleolus 3.75 6.63 5.96 5.32 -1.23 -6.37 7.74 4.14 8.26 -3.50 -4.00 6.87 7.09 11.27 0.17
Nucleus 11.64 0.46 5.13 -4.15 5.96 0.68 -3.23 -6.04 -6.61 -0.18 3.26 -19.44 -3.48 -6.79 14.60
Peroxisomes -3.76 1.21 1.52 7.61 0.44 -7.17 2.64 5.42 9.59 1.33 -8.56 2.47 5.50 12.35 1.41
Vacuole -9.48 -13.10 -10.00 -7.66 -6.45 -5.49 -13.06 -13.51 -10.69 8.66 -8.08 -14.51 -16.37 -12.89 -4.42
Vacuole Periphery -3.51 2.16 2.14 6.07 -0.25 -3.44 3.45 3.57 4.19 0.63 -4.51 3.37 4.05 5.81 0.74
Standard name
Human Ortholog
Description Forkhead transcription factor; drives S-phase activation of genes involved in chromosome segregation, spindle dynamics, budding; also activates genes involved in respiration, use of alternative energy sources (like proline), NAD synthesis, oxidative stress resistance; key factor in early adaptation to nutrient deficiency and diauxic shift; suppressor of calmodulin mutants with specific SPB assembly defects; ortholog of C. elegans lifespan regulator PHA-4
Localization
Cell Percentages nucleus (43%), cytoplasm (20%), mixed (30%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Hcm1

Hcm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hcm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available