Standard name | |
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Human Ortholog | |
Description | Integral ER membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; SED4 has a paralog, SEC12, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 101 | 33 | 79 | 75 | 83 | 69 | 43 | 56 | 26 | 31 | 29 | 7 | 43 | 26 | 35 | 63 | 48 | 58 | 98 | 34 | 121 | 99 | 178 | 128 |
Bud | 9 | 6 | 13 | 12 | 21 | 19 | 33 | 26 | 34 | 30 | 31 | 31 | 8 | 5 | 2 | 13 | 13 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 3 | 1 | 4 | 3 | 0 | 3 | 6 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 2 | 3 | 0 | 0 | 0 | 2 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 21 | 10 | 28 | 25 | 32 | 43 | 25 | 21 | 15 | 11 | 17 | 17 | 19 | 10 | 14 | 20 | 10 | 17 | 20 | 10 | 27 | 23 | 71 | 49 |
Cytoplasm | 49 | 30 | 16 | 18 | 37 | 21 | 28 | 28 | 34 | 44 | 66 | 73 | 46 | 44 | 77 | 160 | 144 | 78 | 8 | 8 | 0 | 2 | 4 | 2 |
Endoplasmic Reticulum | 256 | 70 | 124 | 56 | 112 | 179 | 126 | 107 | 59 | 85 | 47 | 42 | 107 | 55 | 76 | 26 | 20 | 10 | 233 | 70 | 78 | 59 | 138 | 159 |
Endosome | 3 | 1 | 8 | 7 | 18 | 2 | 12 | 20 | 8 | 2 | 4 | 14 | 1 | 0 | 5 | 18 | 20 | 12 | 9 | 3 | 3 | 4 | 6 | 10 |
Golgi | 1 | 0 | 1 | 6 | 3 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 19 | 8 | 9 | 7 | 18 | 24 |
Mitochondria | 14 | 23 | 49 | 74 | 99 | 193 | 314 | 301 | 273 | 371 | 267 | 279 | 1 | 0 | 1 | 35 | 35 | 17 | 11 | 10 | 13 | 22 | 25 | 28 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 5 | 3 | 2 | 2 | 0 | 0 | 1 | 2 | 5 | 3 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 1 | 3 | 1 | 1 | 5 | 8 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 21 | 28 | 67 | 10 | 27 | 20 | 17 | 9 | 11 | 8 | 0 | 0 | 1 | 3 | 11 | 13 | 2 | 0 | 7 | 4 | 6 | 2 |
SpindlePole | 5 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 1 | 3 | 2 | 0 | 0 | 45 | 19 | 20 | 2 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 1 | 0 | 0 | 3 | 2 | 5 | 14 | 23 | 23 | 30 | 33 | 1 | 1 | 3 | 8 | 11 | 14 | 7 | 4 | 0 | 3 | 6 | 6 |
Unique Cell Count | 356 | 128 | 220 | 180 | 315 | 347 | 457 | 424 | 367 | 477 | 364 | 406 | 186 | 119 | 190 | 323 | 284 | 207 | 426 | 156 | 268 | 236 | 471 | 427 |
Labelled Cell Count | 463 | 175 | 341 | 301 | 476 | 542 | 619 | 603 | 504 | 623 | 511 | 517 | 228 | 142 | 215 | 407 | 341 | 257 | 426 | 156 | 268 | 236 | 471 | 427 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 6.4 | 6.8 | 6.7 | 6.8 | 5.8 | 5.5 | 5.7 | 5.3 | 4.8 | 4.9 | 5.2 | 7.3 | 7.3 | 7.9 | 5.5 | 5.9 | 7.2 | 7.7 | 8.0 | 8.6 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.5 | 1.4 | 1.9 | 1.0 | 1.6 | 1.7 | 1.2 | 1.3 | 1.0 | 1.3 | 0.7 | 0.8 | 1.1 | 1.2 | 1.2 | 1.6 | 1.1 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.01 | -0.23 | -0.3 | -0.24 | -0.36 | -0.49 | -0.48 | -0.4 | 0.1 | 0.1 | 0.22 | -0.29 | -0.2 | 0.09 | 0.18 | 0.24 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.2 | -2.4 | -4.2 | -8.4 | -6.7 | -8.8 | 0 | 0 | -11.9 | -2.8 | -2.7 | -3.9 | -4.3 | -4.9 | -1.7 |
Bud | 0.3 | 0.4 | -0.2 | 0.6 | 0.1 | 1.4 | 0 | 0 | 0.8 | -0.7 | -0.7 | -2.6 | -1.0 | -0.7 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.3 | -0.9 | -0.1 | -3.3 | -3.5 | -3.9 | 0 | 0 | -3.9 | -0.8 | -1.2 | -1.8 | -2.6 | -3.9 | -1.5 |
Cytoplasm | 1.0 | 1.7 | -0.6 | -0.6 | -0.3 | 0.8 | 0.9 | 3.7 | 3.7 | 4.9 | 6.8 | 8.0 | 10.3 | 10.4 | 7.6 |
Endoplasmic Reticulum | -5.1 | -4.8 | -1.1 | -7.3 | -7.8 | -10.2 | 0 | 0 | -12.5 | 0.2 | -1.8 | -3.3 | -12.4 | -12.2 | -11.5 |
Endosome | 0.1 | 1.1 | 0 | -0.7 | 0.6 | -1.0 | 0 | 0 | -0.1 | 0 | 0 | -0.6 | 1.0 | 1.7 | 1.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.1 | 2.3 | 7.8 | 11.3 | 11.8 | 12.3 | 0 | 0 | 11.1 | -6.6 | -5.6 | -6.7 | -3.6 | -3.0 | -4.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.8 | 3.6 | -3.4 | -1.7 | -2.4 | -2.3 | 0 | 0 | -4.3 | -4.3 | -3.5 | -4.0 | -4.8 | -2.6 | -1.2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.5 | 3.6 | 4.4 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 3.8 | 3.3 | 4.4 | 4.3 | 0 | 0 | 0 | 0 | 0 | 3.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0131 | 3.413 | 3.1889 | 2.8017 | 2.4308 | 3.1346 | 4.2983 | 5.0197 | 4.6584 | 4.2806 | 4.146 | 4.8875 | 4.5112 | 4.6232 | 4.8115 | 4.6455 | 3.8605 | 4.624 |
Actin | 0.3162 | 0.1951 | 0.2777 | 0.2646 | 0.325 | 0.2779 | 0.2519 | 0.2676 | 0.3976 | 0.2908 | 0.2733 | 0.4166 | 0.2814 | 0.2889 | 0.4325 | 0.3089 | 0.5911 | 0.3198 |
Bud | 0.0007 | 0.0009 | 0.0017 | 0.0012 | 0.0018 | 0.0038 | 0.0003 | 0.0005 | 0.0011 | 0.0004 | 0.001 | 0.0008 | 0.0002 | 0.0004 | 0.0011 | 0.0029 | 0.0008 | 0.0009 |
Bud Neck | 0.0054 | 0.0051 | 0.0084 | 0.0096 | 0.0079 | 0.03 | 0.0055 | 0.0053 | 0.0084 | 0.0043 | 0.0057 | 0.0451 | 0.0027 | 0.0051 | 0.0124 | 0.004 | 0.0044 | 0.0394 |
Bud Periphery | 0.0012 | 0.0018 | 0.0078 | 0.011 | 0.0018 | 0.0033 | 0.0006 | 0.0006 | 0.0049 | 0.0006 | 0.0035 | 0.0022 | 0.0004 | 0.0005 | 0.0061 | 0.0093 | 0.0023 | 0.0033 |
Bud Site | 0.0051 | 0.02 | 0.015 | 0.0093 | 0.0019 | 0.0041 | 0.008 | 0.0362 | 0.0118 | 0.0017 | 0.0025 | 0.0025 | 0.0023 | 0.0141 | 0.0091 | 0.0017 | 0.0011 | 0.0022 |
Cell Periphery | 0.0065 | 0.02 | 0.0139 | 0.0378 | 0.0066 | 0.0133 | 0.0125 | 0.0167 | 0.0121 | 0.0084 | 0.0254 | 0.0082 | 0.0036 | 0.0108 | 0.0203 | 0.0067 | 0.0167 | 0.0156 |
Cytoplasm | 0.05 | 0.174 | 0.0847 | 0.1411 | 0.0333 | 0.1114 | 0.1188 | 0.1983 | 0.0741 | 0.1405 | 0.0695 | 0.0938 | 0.0302 | 0.1503 | 0.0563 | 0.0784 | 0.0878 | 0.1298 |
Cytoplasmic Foci | 0.0724 | 0.0784 | 0.0797 | 0.0876 | 0.0942 | 0.0758 | 0.1153 | 0.062 | 0.0661 | 0.0723 | 0.0896 | 0.0758 | 0.0577 | 0.0671 | 0.051 | 0.0679 | 0.0282 | 0.0476 |
Eisosomes | 0.0074 | 0.007 | 0.0113 | 0.003 | 0.0055 | 0.0052 | 0.0054 | 0.0027 | 0.0057 | 0.0096 | 0.0055 | 0.0043 | 0.0035 | 0.0034 | 0.0176 | 0.003 | 0.0039 | 0.0074 |
Endoplasmic Reticulum | 0.0815 | 0.1497 | 0.0935 | 0.0612 | 0.084 | 0.0923 | 0.0689 | 0.165 | 0.0586 | 0.0319 | 0.0784 | 0.0298 | 0.1263 | 0.1451 | 0.0951 | 0.1032 | 0.0932 | 0.0943 |
Endosome | 0.0722 | 0.0521 | 0.0687 | 0.0953 | 0.0488 | 0.0604 | 0.0605 | 0.029 | 0.0459 | 0.0507 | 0.0618 | 0.0412 | 0.0678 | 0.0376 | 0.0274 | 0.0356 | 0.0127 | 0.0362 |
Golgi | 0.2202 | 0.1631 | 0.2074 | 0.1745 | 0.1374 | 0.1959 | 0.07 | 0.1317 | 0.1718 | 0.2352 | 0.1921 | 0.1688 | 0.1559 | 0.1372 | 0.1732 | 0.2079 | 0.1068 | 0.1622 |
Lipid Particles | 0.1076 | 0.09 | 0.0893 | 0.0596 | 0.1237 | 0.0968 | 0.2062 | 0.0681 | 0.1088 | 0.0491 | 0.1588 | 0.0692 | 0.2491 | 0.1233 | 0.067 | 0.086 | 0.0336 | 0.1107 |
Mitochondria | 0.0139 | 0.0124 | 0.0094 | 0.0074 | 0.017 | 0.0109 | 0.0035 | 0.0011 | 0.0036 | 0.002 | 0.0066 | 0.0091 | 0.002 | 0.0007 | 0.0063 | 0.0229 | 0.0049 | 0.0079 |
None | 0.018 | 0.0195 | 0.0112 | 0.0097 | 0.0721 | 0.0064 | 0.0159 | 0.0034 | 0.0214 | 0.0869 | 0.0167 | 0.0174 | 0.0015 | 0.0069 | 0.0047 | 0.0017 | 0.0019 | 0.0138 |
Nuclear Periphery | 0.0025 | 0.002 | 0.0015 | 0.0015 | 0.0049 | 0.0014 | 0.0176 | 0.0026 | 0.001 | 0.002 | 0.0011 | 0.0008 | 0.0029 | 0.0024 | 0.0014 | 0.0016 | 0.0005 | 0.0012 |
Nucleolus | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0011 | 0.0001 | 0.0023 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Nucleus | 0.0004 | 0.0005 | 0.0003 | 0.0006 | 0.001 | 0.0005 | 0.0035 | 0.0003 | 0.0002 | 0.0009 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0004 |
Peroxisomes | 0.0137 | 0.0053 | 0.0144 | 0.0169 | 0.0256 | 0.0062 | 0.0234 | 0.0056 | 0.0042 | 0.0028 | 0.0044 | 0.0103 | 0.0084 | 0.0029 | 0.0159 | 0.0547 | 0.0086 | 0.0032 |
Punctate Nuclear | 0.0018 | 0.0008 | 0.001 | 0.0018 | 0.0033 | 0.0011 | 0.005 | 0.0007 | 0.0008 | 0.0065 | 0.0006 | 0.0015 | 0.0012 | 0.0012 | 0.0008 | 0.0006 | 0.0004 | 0.0007 |
Vacuole | 0.002 | 0.0019 | 0.0021 | 0.0048 | 0.0019 | 0.0023 | 0.0039 | 0.0023 | 0.0015 | 0.0028 | 0.0022 | 0.0014 | 0.0021 | 0.0014 | 0.0011 | 0.0018 | 0.0007 | 0.0024 |
Vacuole Periphery | 0.001 | 0.0005 | 0.0008 | 0.0014 | 0.0012 | 0.0009 | 0.001 | 0.0003 | 0.0004 | 0.0004 | 0.0006 | 0.0006 | 0.0005 | 0.0003 | 0.0003 | 0.0009 | 0.0002 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.6404 | 26.5254 | 36.1618 | 42.2966 | 25.9461 | 31.0355 | 39.0603 | 42.3072 | 39.0041 | 42.6119 |
Translational Efficiency | 0.9498 | 0.9728 | 0.8689 | 0.7003 | 0.8604 | 1.0781 | 0.6789 | 0.6525 | 0.8831 | 0.7372 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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