Standard name | |
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Human Ortholog | |
Description | Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 1 | 2 | 6 | 9 | 4 | 3 | 12 | 2 | 4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Bud | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 0 |
Bud Neck | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 3 | 4 | 3 | 5 | 7 | 7 | 13 | 2 | 9 | 1 | 1 | 0 | – | 1 | 8 | 8 | 7 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | – | 163 | 164 | 77 | 77 | 124 | 114 | 171 | 87 | 115 | 59 | 52 | 15 | – | 62 | 225 | 172 | 163 | 4 | 162 | 153 | 52 | 78 | 57 |
Endoplasmic Reticulum | – | 4 | 4 | 0 | 5 | 9 | 21 | 27 | 13 | 12 | 5 | 5 | 1 | – | 0 | 6 | 7 | 3 | 0 | 3 | 6 | 1 | 2 | 4 |
Endosome | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 4 | – | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 0 |
Golgi | – | 0 | 1 | 1 | 1 | 2 | 10 | 7 | 5 | 4 | 1 | 4 | 0 | – | 0 | 11 | 14 | 5 | 0 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | – | 7 | 0 | 2 | 16 | 9 | 9 | 4 | 41 | 38 | 32 | 38 | 0 | – | 1 | 12 | 5 | 2 | 0 | 11 | 3 | 0 | 1 | 1 |
Nucleus | – | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 2 |
Nucleolus | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 6 | 2 | 1 | 7 | 1 | 9 | 14 | 7 | 8 | 4 | 8 | 0 | – | 1 | 10 | 14 | 10 | 1 | 5 | 2 | 0 | 3 | 1 |
Unique Cell Count | 175 | 171 | 85 | 102 | 149 | 158 | 219 | 141 | 170 | 93 | 94 | 19 | 65 | 248 | 192 | 178 | 8 | 200 | 182 | 62 | 96 | 74 | ||
Labelled Cell Count | 183 | 176 | 89 | 120 | 159 | 179 | 240 | 161 | 200 | 108 | 114 | 20 | 66 | 273 | 221 | 190 | 8 | 200 | 182 | 62 | 96 | 74 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 11.6 | 15.9 | 17.5 | 12.5 | 16.9 | 15.5 | 15.1 | 12.3 | 12.9 | 11.7 | 12.3 | 12.7 | – | 14.9 | 22.5 | 29.6 | 27.9 | 14.6 | 14.1 | 14.1 |
Std Deviation (1e-4) | – | 4.1 | 5.4 | 6.8 | 6.1 | 6.3 | 5.4 | 5.1 | 5.3 | 3.9 | 4.5 | 4.8 | 7.5 | – | 6.3 | 7.9 | 11.9 | 11.5 | 5.5 | 7.3 | 5.8 |
Intensity Change (Log2) | – | – | – | 0.14 | -0.35 | 0.09 | -0.04 | -0.07 | -0.37 | -0.29 | -0.44 | -0.37 | -0.32 | – | -0.09 | 0.51 | 0.9 | 0.81 | -0.12 | -0.17 | -0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.2 | 1.1 | 1.7 | 0 | 1.4 | 0 | 0 | 0 | – | 0 | 0 | 1.0 | 0.9 |
Cytoplasm | -1.7 | -5.1 | -3.8 | -5.9 | -5.0 | -7.6 | -6.8 | -7.0 | -8.1 | -3.0 | – | -0.2 | -2.0 | -2.3 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 1.7 | 3.7 | 3.6 | 2.7 | 2.1 | 0 | 0 | 0 | – | 0 | 0 | 0.7 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.2 | 0 |
Mitochondria | 0 | 5.3 | 0 | 0 | 0 | 7.6 | 6.6 | 8.2 | 9.0 | 0 | – | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.3 | 2.6 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.8 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0035 | 0.0011 | 0.008 | 0.0007 | 0.0016 | 0.0069 | – | – | – | – | – | – | 0.0332 | 0.0055 | 0.0016 | 0.0014 | 0.0012 | 0.0115 |
Bud | 0.0059 | 0.002 | 0.0162 | 0.0005 | 0.0065 | 0.0058 | – | – | – | – | – | – | 0.0043 | 0.0005 | 0.0017 | 0.0019 | 0.0091 | 0.004 |
Bud Neck | 0.0034 | 0.0007 | 0.0014 | 0.0005 | 0.0007 | 0.0025 | – | – | – | – | – | – | 0.0026 | 0.0005 | 0.0016 | 0.0007 | 0.001 | 0.0025 |
Bud Periphery | 0.0064 | 0.0011 | 0.0266 | 0.0001 | 0.0098 | 0.0089 | – | – | – | – | – | – | 0.0031 | 0.0002 | 0.0031 | 0.0004 | 0.0278 | 0.0044 |
Bud Site | 0.0029 | 0.002 | 0.0047 | 0.0005 | 0.0006 | 0.0026 | – | – | – | – | – | – | 0.0192 | 0.0011 | 0.0063 | 0.0003 | 0.0009 | 0.0006 |
Cell Periphery | 0.008 | 0.0058 | 0.0048 | 0.0003 | 0.0023 | 0.0026 | – | – | – | – | – | – | 0.0085 | 0.0023 | 0.0061 | 0.0004 | 0.0031 | 0.0007 |
Cytoplasm | 0.5427 | 0.9375 | 0.6192 | 0.1893 | 0.4601 | 0.6536 | – | – | – | – | – | – | 0.6545 | 0.9457 | 0.8917 | 0.8445 | 0.8136 | 0.8185 |
Cytoplasmic Foci | 0.0164 | 0.0027 | 0.0229 | 0.088 | 0.0217 | 0.0158 | – | – | – | – | – | – | 0.0193 | 0.0024 | 0.0027 | 0.0065 | 0.0029 | 0.0345 |
Eisosomes | 0.0007 | 0 | 0.0019 | 0 | 0.0001 | 0 | – | – | – | – | – | – | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0571 | 0.0065 | 0.0054 | 0.0025 | 0.0041 | 0.0404 | – | – | – | – | – | – | 0.1092 | 0.0068 | 0.0089 | 0.0027 | 0.0438 | 0.0293 |
Endosome | 0.0365 | 0.004 | 0.0599 | 0.3238 | 0.0492 | 0.088 | – | – | – | – | – | – | 0.0116 | 0.0023 | 0.0081 | 0.0233 | 0.015 | 0.0435 |
Golgi | 0.0069 | 0.0012 | 0.0194 | 0.0716 | 0.0105 | 0.0207 | – | – | – | – | – | – | 0.0027 | 0.0006 | 0.0007 | 0.0012 | 0.0004 | 0.0079 |
Lipid Particles | 0.0025 | 0.0022 | 0.0156 | 0.0012 | 0.0043 | 0.0011 | – | – | – | – | – | – | 0.0027 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0021 |
Mitochondria | 0.0153 | 0.0013 | 0.0457 | 0.0064 | 0.0148 | 0.0265 | – | – | – | – | – | – | 0.0012 | 0.0002 | 0.0021 | 0.0013 | 0.0075 | 0.0055 |
None | 0.1236 | 0.0002 | 0.0339 | 0.0004 | 0.2535 | 0.0009 | – | – | – | – | – | – | 0.0199 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0086 |
Nuclear Periphery | 0.0164 | 0.0013 | 0.0018 | 0.0067 | 0.0029 | 0.008 | – | – | – | – | – | – | 0.0085 | 0.0014 | 0.0028 | 0.0037 | 0.0076 | 0.0029 |
Nucleolus | 0.004 | 0.0001 | 0.0021 | 0.0001 | 0.0013 | 0.0126 | – | – | – | – | – | – | 0.0007 | 0 | 0.0003 | 0 | 0.0006 | 0.0002 |
Nucleus | 0.0182 | 0.0014 | 0.0024 | 0.0021 | 0.0019 | 0.0182 | – | – | – | – | – | – | 0.0043 | 0.001 | 0.0025 | 0.002 | 0.0045 | 0.0018 |
Peroxisomes | 0.0005 | 0.0001 | 0.0052 | 0.0022 | 0.0022 | 0.0003 | – | – | – | – | – | – | 0.0009 | 0.0025 | 0.0001 | 0.0002 | 0.0001 | 0.0006 |
Punctate Nuclear | 0.0299 | 0 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | – | – | – | – | – | – | 0.0053 | 0 | 0.0001 | 0 | 0.0001 | 0.0008 |
Vacuole | 0.0723 | 0.0258 | 0.0809 | 0.235 | 0.0957 | 0.0529 | – | – | – | – | – | – | 0.0806 | 0.0254 | 0.0449 | 0.0877 | 0.0471 | 0.0146 |
Vacuole Periphery | 0.0268 | 0.003 | 0.0212 | 0.0678 | 0.056 | 0.0312 | – | – | – | – | – | – | 0.0076 | 0.0013 | 0.014 | 0.0217 | 0.0129 | 0.0057 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1674.263 | 1721.5917 | 1953.5174 | 1846.3177 | 2005.1751 | 1324.884 | 1546.7755 | 1442.859 | 1470.1273 | 1531.3353 |
Translational Efficiency | 1.3957 | 1.3988 | 1.3173 | 1.1634 | 1.3978 | 1.9016 | 1.4419 | 1.462 | 1.5177 | 1.6715 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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