ORF
Human Ortholog
Description NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.97 0.97 0.94 0.84 0.78 0.76 0.67 0.73 0.69 0.63 0.99 0.98 0.99 0.93 0.9 0.97 0.94 0.96 0.9 0.96 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.08 0.05 0.13 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.13 0.16 0.17 0.22 0.19 0.21 0.31 0 0 0 0.06 0.1 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 1 5 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Bud 0 0 1 0 0 2 0 2 3 2 1 5 0 0 1 0 2 0 0 0 0 1 4 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 3 4 9 11 7 11 4 4 1 3 3 5 9 0 0 0 0 0 0 0
Cytoplasm 118 195 101 139 264 242 263 247 177 252 130 121 399 556 660 145 180 144 109 188 104 203 418 472
Endoplasmic Reticulum 0 1 1 0 2 9 7 16 7 5 2 2 3 2 1 4 2 2 0 0 0 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 3
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Mitochondria 3 0 0 0 1 6 15 4 20 17 24 10 0 2 0 0 2 0 0 0 1 3 1 2
Nucleus 3 0 1 2 2 4 10 5 5 8 7 7 1 0 0 1 2 0 0 3 3 0 0 2
Nuclear Periphery 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 3
Vac/Vac Membrane 0 0 3 3 10 37 54 54 58 65 40 60 3 10 15 10 20 3 1 0 4 2 1 1
Unique Cell Count 122 197 104 143 280 288 339 325 263 344 188 191 403 566 670 156 199 148 116 196 116 213 439 505
Labelled Cell Count 126 197 107 144 283 305 360 340 278 365 208 210 407 573 680 165 218 151 116 196 116 213 439 505


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 14.6 11.0 11.4 13.4 13.1 13.2 13.7 12.5 13.1 11.7 12.6 17.9 27.3 31.2 27.0 26.8 23.2 8.7 9.4 10.3
Std Deviation (1e-4) 2.0 3.4 2.6 2.3 3.3 3.9 4.1 3.6 4.3 4.5 4.3 5.5 6.6 9.2 10.6 18.5 15.1 10.1 1.7 2.0 2.3
Intensity Change (Log2) – – – 0.05 0.28 0.25 0.26 0.31 0.18 0.25 0.08 0.2 0.7 1.31 1.5 1.29 1.28 1.08 -0.35 -0.23 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -1.1 -3.5 -4.6 -4.8 -6.0 -5.2 -5.6 -6.4 1.5 0.8 1.0 -1.5 -2.1 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.9 0 3.8 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.9 3.5 3.6 4.4 4.0 4.2 5.7 0 0 0 1.3 2.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.5491 12.1502 9.9434 8.2878 8.8936 11.2853 8.9237 13.398 11.1246 11.6685 10.6051 11.6674 13.5418 16.8423 14.8014 13.7681 16.0883 15.1603
Actin 0.0193 0.0025 0.0078 0.0261 0.0079 0.0012 0.0236 0.0004 0.0058 0.0074 0.0072 0.0019 0.0095 0.003 0.0044 0.001 0.0005 0.0003
Bud 0.001 0.0011 0.0052 0.0004 0.0012 0.0002 0.0036 0.0009 0.0007 0.0003 0.0002 0.0008 0.0007 0.0029 0.0017 0.0004 0.0002 0.0002
Bud Neck 0.0032 0.0003 0.0007 0.0006 0.0014 0.001 0.0039 0.0002 0.0015 0.0008 0.0012 0.0013 0.0011 0.0002 0.0006 0.0005 0.0007 0.0007
Bud Periphery 0.0021 0.0003 0.0033 0.0003 0.001 0.0001 0.0017 0.0002 0.0021 0.0002 0.0001 0.0005 0.0005 0.0007 0.0012 0.0001 0 0.0001
Bud Site 0.0041 0.0012 0.0044 0.0006 0.0071 0.0001 0.0034 0.0011 0.0012 0.0003 0.0003 0.0006 0.004 0.0063 0.0018 0.0001 0.0001 0.0001
Cell Periphery 0.0007 0.0001 0.0002 0.0001 0.0003 0 0.0002 0 0.0003 0.0001 0 0.0001 0.0002 0.0001 0.0002 0 0 0
Cytoplasm 0.7517 0.955 0.8501 0.7579 0.7601 0.9287 0.7608 0.981 0.9003 0.8738 0.8226 0.9242 0.8604 0.9712 0.9104 0.8976 0.959 0.9263
Cytoplasmic Foci 0.0449 0.0049 0.0197 0.0139 0.0456 0.0048 0.0323 0.0028 0.0072 0.0468 0.0263 0.0084 0.019 0.0014 0.0109 0.0147 0.0027 0.0061
Eisosomes 0.0004 0 0 0.0003 0.0001 0 0.0002 0 0.0002 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0067 0.0015 0.0025 0.0017 0.0066 0.0015 0.0088 0.0005 0.0029 0.0017 0.0076 0.0028 0.0055 0.0005 0.0015 0.0015 0.0012 0.0018
Endosome 0.0502 0.0017 0.0238 0.0378 0.0487 0.0029 0.0452 0.0003 0.0061 0.0121 0.044 0.0118 0.0169 0.0003 0.0126 0.0222 0.0014 0.0134
Golgi 0.009 0.0003 0.0101 0.0193 0.0212 0.0037 0.0134 0 0.0043 0.0025 0.0064 0.0034 0.005 0 0.0047 0.011 0.0001 0.0027
Lipid Particles 0.0195 0.0006 0.0108 0.0286 0.0192 0.0001 0.0157 0 0.0065 0.0058 0.0177 0.0028 0.0033 0 0.0052 0.0127 0 0.0018
Mitochondria 0.0139 0.0002 0.006 0.0123 0.0072 0.0007 0.0069 0.0001 0.0209 0.0033 0.0044 0.0046 0.0099 0.0002 0.0065 0.0025 0.0001 0.0012
None 0.0102 0.0019 0.0094 0.0251 0.0033 0.0011 0.0058 0.0026 0.0064 0.0167 0.002 0.0017 0.0034 0.0012 0.0019 0.0013 0.0008 0.0006
Nuclear Periphery 0.0071 0.0031 0.0035 0.0075 0.0154 0.0108 0.0122 0.001 0.0031 0.0015 0.005 0.0049 0.01 0.0012 0.0026 0.0089 0.0034 0.0042
Nucleolus 0.0008 0.0001 0.0002 0.0004 0.0007 0.0001 0.0021 0 0.0002 0.0001 0.0002 0.0003 0.001 0.0001 0.0013 0.0001 0 0
Nucleus 0.0234 0.0215 0.0279 0.0486 0.0314 0.0397 0.0271 0.0082 0.0224 0.0125 0.0193 0.023 0.0386 0.0091 0.0253 0.017 0.0273 0.0365
Peroxisomes 0.0108 0.0003 0.0087 0.0125 0.0056 0.0007 0.0123 0.0001 0.0022 0.009 0.029 0.0011 0.0026 0.0001 0.0021 0.0023 0.0001 0.0014
Punctate Nuclear 0.011 0.001 0.0022 0.0043 0.0059 0.0012 0.011 0.0005 0.004 0.0037 0.0043 0.0017 0.0024 0.0011 0.0021 0.0006 0.0012 0.0009
Vacuole 0.0076 0.0021 0.0028 0.0011 0.0079 0.0009 0.0076 0.0002 0.0011 0.0009 0.0014 0.0031 0.0034 0.0004 0.0023 0.0038 0.0009 0.0012
Vacuole Periphery 0.0021 0.0002 0.0006 0.0007 0.0023 0.0006 0.0023 0 0.0006 0.0004 0.0007 0.0011 0.0025 0.0001 0.0005 0.0018 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 192.1263 140.7608 188.6347 260.1797 141.69 240.759 197.2875 249.107 293.1796 183.2344
Translational Efficiency 1.5889 1.3673 1.2482 1.3489 1.045 1.5283 1.2821 1.1769 1.3375 1.193

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
200 464 1859 1750 1645 1130 117 1505 1845 1594 1976 3255

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1021.68 1167.73 1965.15 2210.47 1022.64 1093.33 2233.17 2417.90 1022.54 1114.99 1981.02 2306.38
Standard Deviation 166.63 191.65 458.12 672.77 270.36 215.50 512.04 605.37 261.12 211.56 465.80 650.75
Intensity Change Log 2 — 0.192763 0.943696 1.113410 — 0.096431 1.126795 1.241456 — 0.145378 1.038191 1.178883

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000261 0.000335 0.000126 0.001695 0.000227 0.000343 0.001434 0.000247 0.000230 0.000341 0.000203 0.001025
Bud Neck 0.001818 0.000953 0.000694 0.003510 0.000905 0.001814 0.007448 0.002193 0.001004 0.001563 0.001094 0.002901
Bud Site 0.008518 0.007723 0.001287 0.018878 0.004938 0.012104 0.009936 0.008754 0.005326 0.010829 0.001799 0.014197
Cell Periphery 0.000135 0.000100 0.000109 0.000245 0.000105 0.000085 0.000711 0.000221 0.000108 0.000089 0.000145 0.000234
Cytoplasm 0.741344* 0.815955* 0.628889* 0.665543* 0.829972* 0.802999* 0.376331* 0.709987* 0.820365* 0.806771* 0.613935* 0.686093*
Cytoplasmic Foci 0.119866 0.054679 0.003543 0.008632 0.046392 0.057776 0.021887 0.009305 0.054357 0.056875 0.004629 0.008943
Eisosomes 0.000034 0.000013 0.000003 0.000004 0.000010 0.000014 0.000030 0.000003 0.000013 0.000014 0.000005 0.000004
Endoplasmic Reticulum 0.000860 0.000684 0.000058 0.001315 0.001014 0.000602 0.002204 0.000135 0.000998 0.000626 0.000186 0.000770
Endosome 0.007782 0.003096 0.000051 0.004923 0.001709 0.004650 0.005131 0.003166 0.002367 0.004198 0.000352 0.004111
Golgi 0.000658 0.000384 0.000121 0.005218 0.001199 0.002095 0.000818 0.006690 0.001141 0.001597 0.000162 0.005898
Lipid Particles 0.000865 0.000432 0.000195 0.001379 0.000525 0.000852 0.004785 0.000670 0.000562 0.000730 0.000466 0.001051
Mitochondria 0.000434 0.000240 0.000173 0.000484 0.000614 0.002723 0.000314 0.002241 0.000594 0.002000 0.000181 0.001296
Mitotic Spindle 0.000529 0.000686 0.001228 0.065691 0.003344 0.011759 0.003601 0.030301 0.003039 0.008536 0.001369 0.049328
None 0.001293 0.004300 0.012590 0.008857 0.006845 0.003532 0.004432 0.014336 0.006243 0.003755 0.012107 0.011390
Nuclear Periphery 0.000104 0.000095 0.000127 0.000319 0.000133 0.000120 0.001385 0.000270 0.000130 0.000113 0.000202 0.000296
Nuclear Periphery Foci 0.004086 0.004104 0.000470 0.002062 0.002864 0.002587 0.015384 0.001609 0.002997 0.003029 0.001353 0.001853
Nucleolus 0.000149 0.000105 0.000078 0.000259 0.000147 0.000141 0.000275 0.000212 0.000147 0.000130 0.000090 0.000237
Nucleus 0.037898 0.060135 0.345868* 0.180829* 0.074376 0.051000 0.270176* 0.180138* 0.070422 0.053659 0.341386* 0.180509*
Peroxisomes 0.000796 0.000416 0.000021 0.000247 0.000186 0.002022 0.000280 0.000098 0.000252 0.001554 0.000036 0.000178
Vacuole 0.071954 0.045244 0.004333 0.026580 0.023877 0.041586 0.273127* 0.024714 0.029089 0.042651 0.020248 0.025717
Vacuole Periphery 0.000615 0.000323 0.000033 0.003330 0.000617 0.001195 0.000312 0.004708 0.000617 0.000941 0.000049 0.003967

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.74 0.72 -1.76 -1.19 -2.03 -1.65 -7.56 -0.33 1.90 7.95 -1.75 -1.18 -1.82 -0.65 -1.29
Bud Neck 2.03 1.10 -2.66 -6.67 -5.30 -3.35 -8.35 -9.08 -3.53 6.63 -2.80 -5.06 -10.30 -5.53 -4.28
Bud Site 0.63 6.72 0.55 -0.16 -6.42 -4.29 -6.52 -1.33 3.45 5.68 -4.21 4.21 -2.18 2.70 -7.05
Cell Periphery 1.64 -2.76 -4.79 -7.11 -2.62 1.80 -6.29 -8.94 -9.92 4.71 1.87 -7.67 -10.94 -12.70 -2.13
Cytoplasm -3.56 7.66 4.16 13.38 -8.53 5.44 26.50 22.96 13.42 -18.10 4.05 36.57 26.71 18.91 -13.42
Cytoplasmic Foci 5.68 11.30 10.99 11.57 -3.83 -3.06 3.38 15.15 14.99 3.96 -0.87 20.92 19.46 19.42 -5.17
Eisosomes 4.35 7.09 7.03 6.53 -1.10 -3.30 -8.63 8.11 10.47 11.63 -1.26 5.96 10.41 12.29 0.45
Endoplasmic Reticulum 0.71 4.80 2.35 1.97 -1.05 2.40 -0.98 6.19 6.58 6.60 2.39 6.02 4.80 3.77 -0.21
Endosome 1.70 2.95 2.56 3.55 -3.95 -3.39 -2.50 3.78 4.71 4.43 -2.44 6.80 4.13 5.47 -4.09
Golgi 1.39 2.64 -1.02 -1.82 -2.42 -0.90 0.70 -0.22 0.64 -0.96 -0.44 2.16 -0.11 0.40 -3.21
Lipid Particles 2.95 1.74 -5.14 -9.14 -7.53 -1.29 -8.22 -8.66 -3.56 6.17 -0.94 -3.30 -11.36 -5.62 -6.64
Mitochondria 0.53 0.60 0.55 -0.09 -0.72 -1.42 0.81 0.24 1.55 -0.78 -1.27 1.13 0.67 1.65 -1.23
Mitotic Spindle -0.38 -4.24 -8.35 -8.26 -7.66 -3.13 -1.41 -4.67 -0.80 -2.57 -2.74 0.85 -8.16 -4.28 -9.27
None -3.87 -26.62* -14.68 -4.05 9.70 4.83 3.91 -2.07 -7.23 -5.86 4.22 -7.11 -2.29 -8.93 6.61
Nuclear Periphery 0.02 -8.03 -17.02 -18.25 -12.11 -0.69 -11.57 -13.24 -14.69 8.94 -0.22 -9.20 -17.68 -22.37 -8.33
Nuclear Periphery Foci -0.08 5.43 4.06 5.32 -5.16 0.47 -5.43 2.53 1.91 6.16 -0.41 6.44 4.46 5.03 -3.27
Nucleolus 1.76 1.65 -1.45 -2.92 -2.79 0.33 -4.42 -2.14 -2.03 2.47 1.00 1.70 -2.47 -2.96 -3.39
Nucleus -4.97 -59.21 -29.76 -22.51 30.13 5.59 -8.77 -25.55 -29.62 2.88 4.42 -57.58 -33.53 -38.03 32.49
Peroxisomes 1.31 3.15 2.84 2.79 -4.18 -2.78 -1.22 7.19 3.04 3.57 -2.77 6.77 5.63 3.24 -2.99
Vacuole 2.38 7.33 -1.78 -6.84 -22.38 -9.05 -12.72 -15.30 -6.24 10.10 -7.95 -0.29 -19.67 -9.87 -17.02
Vacuole Periphery 0.96 1.94 -0.15 -1.39 -2.67 -0.70 1.36 -0.68 0.19 -1.94 -0.45 2.39 -0.49 0.10 -3.65
ORF
Human Ortholog
Description NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YDL124W

YDL124W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydl124w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available