Standard name
Human Ortholog
Description Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.23 0.12 0.18 0.11 0.13 0.18
Bud 0.06 0 0.06 0 0.05 0.06 0.06 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.06 0.06 0.05 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0.05 0.09 0 0 0 0 0 0
Cytoplasm 0.82 0.9 0.89 0.85 0.92 0.74 0.64 0.65 0.61 0.61 0.46 0.38 0.99 0.99 0.99 0.82 0.89 0.86 0.55 0.62 0.45 0.62 0.65 0.57
Endoplasmic Reticulum 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0 0 0.2 0.05 0.34 0.48 0.34 0.55 0.51 0.68 0.75 0 0 0 0 0 0 0.05 0 0.07 0.08 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 5 5 0 2 2 0 1 0 0 1 1 1 1 0 14 10 6 46 15 18 17 31 55
Bud 11 4 4 1 5 4 7 8 1 5 5 4 0 0 0 7 4 2 4 4 1 2 5 9
Bud Neck 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 11 11 6 9 11 17
Bud Site 0 0 1 0 0 1 0 1 1 2 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 0 1 2 0 0 0 0 3 1 2 13 11 19 17 9 10 2 3 1 1 4 11
Cytoplasm 155 89 64 34 88 48 73 106 59 73 32 42 237 290 302 253 155 101 114 79 46 97 156 176
Endoplasmic Reticulum 4 9 2 1 2 0 2 1 1 1 0 3 1 0 2 21 6 6 1 1 2 3 7 7
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 17 3 2 7 1 6 5 4 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0
Mitochondria 36 0 2 8 5 22 55 55 53 61 47 83 0 0 0 12 0 2 10 4 7 12 10 11
Nucleus 0 1 1 0 1 0 0 1 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0
Nuclear Periphery 4 0 0 0 0 0 1 1 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 1 0 0 4 4 1 2 1 0 0 0 0 6 3 3 2 0 3 0 0 2
Vac/Vac Membrane 2 0 0 0 0 0 1 3 0 2 0 1 0 0 0 10 4 4 1 3 0 1 3 7
Unique Cell Count 188 99 72 40 96 65 114 162 97 119 69 111 240 293 304 307 175 117 206 128 102 156 240 312
Labelled Cell Count 223 112 81 47 105 77 143 183 116 151 88 139 254 304 323 368 197 138 206 128 102 156 240 312


Ambiguous, Bud, Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.6 4.6 4.2 4.9 4.6 3.3 3.7 3.7 3.6 3.3 3.0 6.6 7.2 7.9 6.7 7.7 7.7 4.9 5.5 6.4
Std Deviation (1e-4) 0.4 0.6 1.1 1.0 2.0 2.5 1.0 1.3 1.6 2.0 0.9 0.8 1.1 1.1 1.5 1.6 1.6 1.9 1.2 1.2 1.1
Intensity Change (Log2) – – – -0.11 0.1 0.02 -0.45 -0.31 -0.31 -0.35 -0.48 -0.6 0.53 0.66 0.8 0.55 0.75 0.75 0.11 0.28 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 0.6 -2.3 -3.7 -3.7 -4.1 -4.1 -5.4 -6.8 4.0 4.5 5.0 -1.3 -0.1 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0