ORF
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0.12 0.08 0.09 0 0.05 0 0.06 0.05 0
Bud 0 0 0.08 0.06 0.05 0.05 0 0.06 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.56 0.51 0.54 0.49 0.44 0.39 0.37 0.43 0.35 0.32 0.66 0.62 0.68 0.58 0.54 0.55 0.4 0.15 0.11 0.1 0.07 0.11
Endoplasmic Reticulum 0.11 0.09 0.05 0 0 0 0 0 0 0 0 0.15 0.09 0.07 0 0 0.05 0.07 0.05 0.05 0.07 0 0.05
Endosome 0 0.05 0 0 0.25 0.05 0 0 0 0 0 0.07 0.11 0.12 0.08 0.16 0.12 0 0.06 0.05 0.07 0.09 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Mitochondria 0.07 0.19 0.46 0.55 0.24 0.61 0.64 0.73 0.68 0.76 0.78 0.06 0.06 0.06 0 0 0.08 0.23 0.35 0.58 0.46 0.47 0.39
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0.12 0 0 0.09 0 0 0 0 0 0 0.06 0.06 0.06 0.17 0.17 0.11 0.13 0.23 0.08 0.11 0.16 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 6 3 1 0 0 0 0 0 0 0 13 7 10 14 10 10 15 7 11 13 13 14
Bud 6 2 25 18 19 24 29 44 37 35 45 4 1 7 4 3 3 7 3 7 4 5 7
Bud Neck 1 0 4 0 0 0 5 2 2 0 3 0 0 0 0 0 0 22 6 8 4 7 11
Bud Site 0 0 0 0 1 3 3 9 5 5 21 0 0 0 0 0 0
Cell Periphery 6 2 5 7 4 2 1 1 4 1 2 1 10 3 7 4 4 3 0 3 1 1 0
Cytoplasm 304 67 166 158 194 228 266 271 293 182 245 141 170 196 66 65 63 194 24 40 21 18 34
Endoplasmic Reticulum 42 11 16 2 2 4 6 1 1 2 4 32 25 19 2 4 6 35 7 21 14 10 16
Endosome 4 6 0 9 97 24 12 8 12 0 4 15 31 34 9 19 14 20 9 20 16 24 21
Golgi 0 1 0 1 1 0 0 0 0 0 0 2 1 1 1 1 4 1 2 4 10 11 11
Mitochondria 29 23 152 161 94 316 432 540 468 397 597 12 17 18 1 3 9 110 55 224 102 127 125
Nucleus 1 2 0 2 0 2 8 4 4 4 6 1 1 0 2 2 2 1 0 0 0 0 0
Nuclear Periphery 1 1 2 0 0 0 4 2 1 1 0 0 0 0 1 1 0 1 1 2 0 1 2
Nucleolus 0 0 0 0 0 0 3 0 1 2 3 0 0 0 2 2 1 0 0 0 0 0 1
Peroxisomes 0 0 0 2 11 4 3 5 3 2 6 0 7 4 2 2 0 0 0 2 1 1 0
SpindlePole 2 0 5 3 11 11 32 24 34 18 28 2 1 1 3 3 6 4 0 4 2 2 4
Vac/Vac Membrane 9 14 9 2 36 22 26 15 11 12 13 12 17 16 19 20 13 65 36 29 25 43 61
Unique Cell Count 390 119 327 292 395 518 675 735 686 523 765 215 275 290 114 121 114 492 159 389 225 272 319
Labelled Cell Count 415 135 387 366 470 640 830 926 876 661 977 235 288 309 133 139 135 492 159 389 225 272 319


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.8 3.9 4.2 4.9 3.8 3.6 3.1 3.3 3.1 3.1 6.4 6.1 5.8 8.2 7.5 7.2 6.2 6.4 6.5
Std Deviation (1e-4) 0.6 1.0 0.9 1.1 1.7 1.1 1.2 1.1 0.9 0.8 0.9 1.1 1.2 1.4 1.7 1.5 1.6 1.5 1.9 2.5
Intensity Change (Log2) 0.11 0.34 -0.02 -0.11 -0.33 -0.23 -0.34 -0.32 0.71 0.65 0.58 1.07 0.94 0.89 0.67 0.71 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.5 0 2.2 0 0 0
Bud 0 -1.6 0 0 0 0 0 0 -2.9 -4.4 -2.9 -1.5 -2.0 -1.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1.7 0 0 0 0
Cytoplasm 0.8 -0.4 -1.9 -3.4 -4.2 -2.4 -4.6 -5.9 3.4 2.7 4.2 1.3 0.6 0.8
Endoplasmic Reticulum 0 -3.8 0 0 0 0 0 0 4.0 2.0 0.9 0 -0.7 0.2
Endosome 0 9.6 0 0 0 0 0 0 4.8 6.2 6.4 0 7.3 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -6.4 0 0 0 0 0 0 -10.1 -11.0 -11.2 -8.8 -8.7 -7.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1.1 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 3.5 1.1 0.9 -0.7 -1.2 -0.4 -1.1 1.7 2.1 1.7 5.2 5.3 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3173 2.3186 1.9475 1.6584 1.1739 1.9183 1.8776 2.0298 2.0864 1.9767 1.8696 1.7508 2.7262 3.6333 3.6466 3.1218 2.2893 3.1653
Actin 0.0155 0.006 0.0072 0.0112 0.0047 0.0094 0.0312 0.0057 0.0303 0.0093 0.0304 0.0129 0.0269 0.011 0.0129 0.006 0.0042 0.0078
Bud 0.0018 0.0031 0.0039 0.0169 0.0078 0.0038 0.0038 0.0051 0.008 0.0093 0.0056 0.0032 0.002 0.0067 0.005 0.0087 0.0043 0.0022
Bud Neck 0.003 0.0043 0.0048 0.0027 0.0045 0.0132 0.0104 0.0027 0.0104 0.0066 0.0056 0.0154 0.0031 0.0053 0.0177 0.0045 0.0022 0.0208
Bud Periphery 0.0039 0.0039 0.0085 0.0471 0.0164 0.0081 0.007 0.008 0.0118 0.0142 0.0169 0.0069 0.0028 0.006 0.0074 0.0235 0.0151 0.0081
Bud Site 0.0151 0.036 0.0199 0.0123 0.0045 0.0065 0.0224 0.0628 0.0347 0.0068 0.0082 0.0034 0.0149 0.0872 0.0383 0.0069 0.005 0.003
Cell Periphery 0.0036 0.0023 0.0044 0.0087 0.0022 0.0036 0.0027 0.0028 0.0032 0.0017 0.0053 0.0021 0.006 0.0048 0.0048 0.0048 0.0085 0.0032
Cytoplasm 0.2863 0.4359 0.4108 0.3238 0.3258 0.3041 0.2133 0.3697 0.2727 0.2963 0.2541 0.3084 0.3186 0.4025 0.4572 0.2761 0.1848 0.332
Cytoplasmic Foci 0.1112 0.0956 0.0967 0.08 0.1671 0.1315 0.1263 0.1167 0.1124 0.0974 0.1184 0.1238 0.1158 0.1076 0.0808 0.0869 0.1361 0.1211
Eisosomes 0.0005 0.0003 0.0005 0.0003 0.0001 0.0004 0.0006 0.0003 0.0006 0.0003 0.0008 0.0003 0.0013 0.0004 0.0004 0.0002 0.001 0.0005
Endoplasmic Reticulum 0.0387 0.0221 0.0249 0.0267 0.0135 0.0275 0.0206 0.0191 0.0267 0.0072 0.0118 0.0095 0.0354 0.0183 0.0331 0.0295 0.0162 0.021
Endosome 0.2276 0.1818 0.1656 0.2146 0.1982 0.171 0.2521 0.186 0.1805 0.1931 0.1598 0.1949 0.1913 0.1571 0.1545 0.192 0.2287 0.2023
Golgi 0.0403 0.0478 0.0486 0.0674 0.0459 0.0503 0.0789 0.0462 0.077 0.0727 0.0498 0.0659 0.0585 0.0536 0.0544 0.0843 0.1012 0.0772
Lipid Particles 0.0154 0.005 0.005 0.0026 0.0041 0.0054 0.019 0.0041 0.0102 0.004 0.0131 0.0074 0.0165 0.0061 0.0062 0.0024 0.0029 0.0062
Mitochondria 0.0638 0.0366 0.0857 0.1075 0.1126 0.1485 0.0925 0.0666 0.1186 0.172 0.1115 0.124 0.0531 0.0271 0.0499 0.1418 0.265 0.1108
None 0.1159 0.0857 0.0779 0.0239 0.059 0.0807 0.0681 0.0685 0.0568 0.0522 0.1385 0.083 0.1099 0.076 0.0447 0.0905 0.0047 0.0589
Nuclear Periphery 0.0056 0.0021 0.0036 0.0048 0.0018 0.0021 0.0085 0.0022 0.0029 0.0097 0.0128 0.0029 0.0064 0.0026 0.0024 0.0014 0.0011 0.0016
Nucleolus 0.0008 0.0005 0.0005 0.0006 0.0005 0.0006 0.0015 0.0027 0.0013 0.0007 0.0017 0.0008 0.0007 0.0007 0.0008 0.0003 0.0002 0.0003
Nucleus 0.0031 0.0023 0.0022 0.0031 0.0024 0.0025 0.0054 0.0038 0.003 0.0033 0.0041 0.0028 0.002 0.0028 0.0023 0.0015 0.0007 0.0021
Peroxisomes 0.0114 0.0032 0.0064 0.0038 0.0045 0.0101 0.0084 0.0044 0.0122 0.0066 0.0205 0.0107 0.0094 0.0047 0.0028 0.0077 0.0044 0.0049
Punctate Nuclear 0.0047 0.001 0.001 0.0006 0.0017 0.0024 0.0038 0.0041 0.0022 0.0024 0.0093 0.0022 0.0032 0.0015 0.0013 0.0004 0.0003 0.0007
Vacuole 0.0263 0.0211 0.0177 0.0316 0.0177 0.0135 0.0182 0.0149 0.019 0.0258 0.0163 0.0144 0.019 0.0155 0.0194 0.0227 0.0079 0.0112
Vacuole Periphery 0.0054 0.0033 0.0044 0.0096 0.005 0.0048 0.0053 0.0033 0.0054 0.0083 0.0055 0.005 0.0031 0.0024 0.0039 0.0081 0.0056 0.0041

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 139.0846 118.8072 98.4113 81.4986 116.2122 169.8982 136.1892 96.1609 105.3115 144.9308
Translational Efficiency 1.8995 1.8513 1.4545 2.3266 2.1334 1.6709 1.5017 2.3333 1.8324 1.8111

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1424 1759 160 1298 1721 1927 2048 1206 3145 3686 2208 2504

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 713.13 794.54 1015.15 1006.39 732.08 812.07 951.10 1053.12 723.50 803.70 955.74 1028.90
Standard Deviation 110.50 111.68 125.73 153.67 94.08 102.54 133.18 152.21 102.28 107.36 133.69 154.74
Intensity Change Log 2 0.155955 0.509456 0.496952 0.149603 0.377596 0.524597 0.152741 0.444168 0.511022

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001737 0.001938 0.001926 0.033439 0.002119 0.001735 0.014378 0.038694 0.001946 0.001832 0.013475 0.035970
Bud Neck 0.057303 0.035387 0.004273 0.012459 0.051401 0.043392 0.027891 0.011873 0.054073 0.039572 0.026179 0.012177
Bud Site 0.029430 0.057436 0.016208 0.202101* 0.031701 0.043589 0.188547* 0.196239* 0.030673 0.050197 0.176059* 0.199278*
Cell Periphery 0.000837 0.000603 0.000547 0.000893 0.000418 0.000323 0.000358 0.000574 0.000608 0.000457 0.000372 0.000740
Cytoplasm 0.218331* 0.131819* 0.308653* 0.149324* 0.185471* 0.143643* 0.250525* 0.132048* 0.200349* 0.138000* 0.254737* 0.141004*
Cytoplasmic Foci 0.307915* 0.309078* 0.134100 0.197278* 0.339664* 0.293967* 0.179478 0.237355* 0.325289* 0.301179* 0.176189 0.216580*
Eisosomes 0.001355 0.000337 0.000182 0.000270 0.000876 0.000392 0.000191 0.000351 0.001093 0.000366 0.000191 0.000309
Endoplasmic Reticulum 0.007658 0.002891 0.019334 0.015852 0.005374 0.002439 0.006911 0.006438 0.006408 0.002655 0.007811 0.011318
Endosome 0.104979 0.113657 0.040943 0.079693 0.107534 0.128100 0.052865 0.085515 0.106377 0.121208 0.052001 0.082497
Golgi 0.040491 0.079852 0.001375 0.062059 0.046955 0.061394 0.038838 0.115166* 0.044028 0.070202 0.036123 0.087637
Lipid Particles 0.035343 0.011199 0.014974 0.007987 0.028299 0.014793 0.004256 0.005414 0.031488 0.013078 0.005033 0.006747
Mitochondria 0.051201 0.102372* 0.000245 0.015640 0.051335 0.102602* 0.053908 0.032646 0.051274 0.102492* 0.050019 0.023831
Mitotic Spindle 0.000305 0.007670 0.002372 0.023625 0.001912 0.006353 0.013581 0.021535 0.001184 0.006982 0.012769 0.022618
None 0.001064 0.001236 0.001224 0.000358 0.000531 0.000679 0.000204 0.000128 0.000772 0.000945 0.000278 0.000247
Nuclear Periphery 0.000410 0.000411 0.001784 0.000465 0.000272 0.000397 0.000173 0.000087 0.000334 0.000404 0.000290 0.000283
Nuclear Periphery Foci 0.000321 0.000658 0.023128 0.002122 0.000329 0.000485 0.000803 0.000529 0.000325 0.000567 0.002420 0.001354
Nucleolus 0.000654 0.000981 0.001121 0.000510 0.000551 0.001264 0.000264 0.000106 0.000598 0.001129 0.000326 0.000315
Nucleus 0.017785 0.007574 0.142300* 0.015841 0.015618 0.011961 0.009725 0.002179 0.016599 0.009867 0.019332 0.009261
Peroxisomes 0.021746 0.028725 0.004999 0.029799 0.026866 0.024683 0.022279 0.032837 0.024548 0.026612 0.021027 0.031262
Vacuole 0.089917 0.088591 0.278940* 0.137472 0.093602 0.098972 0.118996 0.068583 0.091934 0.094018 0.130586 0.104293
Vacuole Periphery 0.011218 0.017583 0.001371 0.012813 0.009173 0.018838 0.015831 0.011703 0.010099 0.018239 0.014783 0.012278

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.61 -0.27 -11.05 -11.00 -10.88 0.87 -9.77 -12.63 -12.72 -7.77 0.42 -9.98 -16.70 -16.74 -9.61
Bud Neck 6.33 18.10 15.16 11.21 -9.62 2.43 8.13 14.94 14.28 10.30 6.08 12.13 21.25 18.12 10.12
Bud Site -8.41 4.31 -24.89 -20.20 -26.66* -4.30 -31.44* -25.16 -22.99 -1.28 -9.09 -31.44 -35.35 -30.54 -3.76
Cell Periphery 1.13 3.12 -0.18 -1.20 -2.90 2.37 1.53 -2.23 -4.09 -3.47 1.49 4.92 -1.51 -2.40 -5.50
Cytoplasm 12.19 -4.13 8.18 -3.20 7.50 6.64 -9.47 7.06 1.16 15.71 13.20 -8.89 10.48 -1.51 17.38
Cytoplasmic Foci -0.20 15.85 16.19 16.87 -5.95 6.55 25.36 14.13 8.34 -8.70 4.72 29.10 21.78 17.82 -7.61
Eisosomes 5.73 6.69 6.19 1.80 -3.06 8.14 12.55 8.29 1.05 -4.31 8.37 10.63 9.08 2.11 -5.12
Endoplasmic Reticulum 5.09 -2.50 -4.40 -7.63 0.57 3.64 -1.58 -0.76 -3.54 0.50 6.11 -1.56 -4.18 -8.36 -2.71
Endosome -1.86 12.75 6.20 8.74 -8.07 -4.52 15.48 5.42 9.89 -9.31 -4.55 19.32 8.34 13.31 -11.19
Golgi -10.98 19.94 -5.06 4.80 -18.52 -4.75 3.84 -13.81 -10.26 -16.10 -11.18 4.53 -13.50 -4.41 -16.11
Lipid Particles 12.05 8.46 13.76 2.92 3.35 7.91 16.58 14.73 7.98 -1.90 14.17 22.21 19.94 7.87 -3.36
Mitochondria -10.09 17.65 11.32 20.00 -10.70 -10.54 -0.23 5.01 15.43 5.75 -14.57 0.90 11.17 25.18 9.28
Mitotic Spindle -8.42 -1.69 -10.08 -6.58 -7.52 -3.61 -8.07 -8.31 -6.13 -2.92 -7.48 -9.20 -12.91 -9.01 -4.76
None -0.33 -0.13 2.26 1.78 1.81 -0.73 2.40 2.82 4.28 2.05 -0.61 3.27 3.23 2.84 0.22
Nuclear Periphery -0.01 -6.51 -0.82 -0.67 6.62 -1.04 1.82 4.35 2.58 3.81 -0.62 0.42 0.16 0.78 -0.31
Nuclear Periphery Foci -1.66 -8.40 -6.35 -4.28 7.61 -1.55 -3.09 -3.24 -0.74 1.56 -2.20 -7.59 -6.90 -4.40 3.07
Nucleolus -1.26 -1.85 0.76 1.58 2.11 -3.65 4.00 6.64 6.02 5.39 -3.25 4.35 2.76 4.52 0.27
Nucleus 8.10 -8.57 0.07 -5.11 8.54 2.57 3.67 11.79 9.31 9.24 7.06 -2.21 4.93 -0.51 5.49
Peroxisomes -3.63 9.15 -3.01 -0.06 -9.84 1.16 2.70 -2.66 -3.76 -5.08 -1.63 2.62 -3.79 -2.47 -5.78
Vacuole 0.19 -11.44 -8.96 -9.11 8.02 -1.12 -6.74 4.06 4.96 10.05 -0.66 -10.73 -4.90 -4.34 5.09
Vacuole Periphery -6.39 15.03 -0.85 4.26 -10.82 -9.95 -5.64 -2.19 6.04 2.94 -11.73 -4.29 -2.23 7.26 2.20
ORF
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (13%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

YDL157C

YDL157C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydl157c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available