Standard name | |
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Human Ortholog | |
Description | Component of the Sec13p-Sec31p complex of the COPII vesicle coat; COPII coat is required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 131 | 112 | 237 | 192 | 146 | 194 | 200 | 121 | 121 | – | 66 | 101 | 88 | 102 | 137 | 114 | 59 | 74 | 170 | 95 | 243 | 71 | 146 | 161 |
Bud | 4 | 4 | 21 | 22 | 23 | 25 | 26 | 18 | 19 | – | 23 | 24 | 6 | 7 | 15 | 7 | 2 | 11 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 7 | 20 | 21 | 18 | 19 | 11 | 3 | 10 | – | 7 | 16 | 5 | 3 | 3 | 6 | 8 | 12 | 0 | 0 | 0 | 0 | 2 | 3 |
Cytoplasm | 13 | 19 | 10 | 9 | 17 | 15 | 30 | 24 | 12 | – | 6 | 10 | 10 | 6 | 11 | 25 | 20 | 37 | 2 | 2 | 0 | 0 | 2 | 0 |
Endoplasmic Reticulum | 209 | 138 | 275 | 116 | 124 | 157 | 221 | 198 | 112 | – | 45 | 60 | 104 | 113 | 99 | 16 | 5 | 17 | 66 | 57 | 42 | 16 | 35 | 16 |
Endosome | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 2 | – | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 |
Golgi | 328 | 124 | 263 | 181 | 235 | 204 | 262 | 190 | 80 | – | 56 | 60 | 128 | 183 | 159 | 254 | 159 | 198 | 139 | 64 | 115 | 45 | 56 | 46 |
Mitochondria | 42 | 18 | 118 | 194 | 153 | 230 | 311 | 382 | 351 | – | 256 | 377 | 32 | 51 | 126 | 15 | 25 | 16 | 4 | 3 | 0 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 2 | 3 | 8 | 7 | 4 | – | 5 | 11 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | – | 0 | 1 | 0 | 0 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 1 | 1 | 5 | 1 | 0 | – | 3 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 9 | 1 | 1 | 1 | 0 | 1 | 1 | 6 | 0 | – | 0 | 0 | 0 | 0 | 1 | 6 | 1 | 3 | 0 | 2 | 0 | 1 | 0 | 0 |
Unique Cell Count | 378 | 226 | 418 | 309 | 332 | 390 | 546 | 552 | 410 | 286 | 417 | 182 | 231 | 268 | 288 | 181 | 253 | 390 | 231 | 413 | 140 | 248 | 235 | |
Labelled Cell Count | 748 | 423 | 947 | 738 | 721 | 849 | 1077 | 954 | 712 | 468 | 665 | 374 | 465 | 557 | 445 | 281 | 371 | 390 | 231 | 413 | 140 | 248 | 235 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 36.3 | 33.4 | 22.4 | 21.9 | 22.1 | 20.4 | 19.8 | 18.4 | 17.1 | – | 15.9 | 15.3 | 29.6 | 29.1 | 26.9 | 33.3 | 36.4 | 39.1 | 29.2 | 32.3 | 31.9 |
Std Deviation (1e-4) | 9.1 | 6.9 | 3.9 | 4.7 | 5.0 | 4.0 | 4.4 | 4.2 | 4.2 | – | 3.7 | 3.7 | 6.9 | 7.5 | 7.4 | 11.8 | 12.9 | 14.5 | 6.3 | 7.9 | 8.4 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.02 | -0.13 | -0.18 | -0.28 | -0.39 | – | -0.49 | -0.55 | 0.4 | 0.38 | 0.26 | 0.57 | 0.7 | 0.8 | 0.38 | 0.52 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.5 | -3.5 | -2.0 | -6.2 | -11.1 | -7.9 | – | -8.8 | -9.6 | -1.9 | -3.1 | -1.4 | -4.5 | -5.4 | -6.9 |
Bud | 1.2 | 1.1 | 0.8 | -0.2 | -1.4 | -0.3 | – | 1.6 | 0.5 | -0.9 | -1.2 | 0.3 | -1.7 | -2.3 | -0.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.2 | 0.4 | 0.1 | -2.4 | -4.3 | -1.8 | – | -1.6 | -0.7 | -1.1 | -2.3 | -2.6 | -1.9 | -0.2 | 0 |
Cytoplasm | 0.4 | 2.0 | 1.2 | 2.4 | 1.6 | 0.5 | – | -0.3 | 0 | 1.9 | 0.2 | 1.3 | 3.8 | 4.5 | 6.0 |
Endoplasmic Reticulum | -7.6 | -7.8 | -7.3 | -7.8 | -9.2 | -11.1 | – | -13.1 | -15.2 | -2.0 | -4.2 | -7.4 | -16.0 | -14.2 | -15.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.2 | 2.3 | -3.1 | -4.6 | -8.8 | -12.7 | – | -11.3 | -14.4 | 1.8 | 4.3 | -0.9 | 7.5 | 6.1 | 4.2 |
Mitochondria | 9.3 | 5.1 | 8.8 | 8.9 | 12.6 | 16.7 | – | 16.0 | 18.3 | -2.8 | -1.7 | 5.0 | -7.7 | -3.8 | -6.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 39.6986 | 47.6429 | 43.9242 | 47.6415 | 41.6402 | 47.3913 | 33.2173 | 44.5992 | 41.7452 | 39.8984 | 41.9118 | 41.1318 | 31.0069 | 39.8802 | 39.9198 | 37.8099 | 33.1209 | 36.3534 |
Actin | 0.1774 | 0.0943 | 0.0845 | 0.0064 | 0.0458 | 0.0341 | 0.1339 | 0.068 | 0.0628 | 0.0394 | 0.0113 | 0.0327 | 0.1888 | 0.1687 | 0.2098 | 0.3256 | 0.0424 | 0.1237 |
Bud | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0006 | 0.0007 | 0.0002 | 0 | 0 | 0.0002 | 0 |
Bud Neck | 0.001 | 0.0003 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0006 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0005 | 0.0008 | 0.0003 | 0.0003 |
Bud Periphery | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0011 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0026 | 0.0002 | 0.0003 | 0.002 | 0 | 0.0006 | 0.0029 | 0.0004 | 0.0001 | 0 | 0.0005 | 0.0001 |
Cell Periphery | 0.0009 | 0.0004 | 0.0007 | 0.0004 | 0.0002 | 0.0004 | 0.0006 | 0.0009 | 0.0005 | 0.0007 | 0.0007 | 0.0004 | 0.0005 | 0.0006 | 0.0005 | 0.0004 | 0.0004 | 0.0002 |
Cytoplasm | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0.0036 | 0.0023 | 0.0029 | 0.0036 | 0 | 0.004 | 0.0021 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0012 |
Cytoplasmic Foci | 0.002 | 0.0024 | 0.0012 | 0.0005 | 0.0008 | 0.0005 | 0.0105 | 0.0029 | 0.0013 | 0.0033 | 0.0008 | 0.0012 | 0.0144 | 0.0022 | 0.0011 | 0.001 | 0.0146 | 0.0034 |
Eisosomes | 0.0597 | 0.0428 | 0.0459 | 0.0212 | 0.0519 | 0.0173 | 0.0641 | 0.0245 | 0.0552 | 0.0432 | 0.0459 | 0.0228 | 0.0393 | 0.0168 | 0.039 | 0.1851 | 0.0731 | 0.0098 |
Endoplasmic Reticulum | 0.0016 | 0.0021 | 0.0024 | 0.0013 | 0.001 | 0.0023 | 0.0053 | 0.0038 | 0.003 | 0.0056 | 0.0032 | 0.0035 | 0.0116 | 0.0012 | 0.0017 | 0.0017 | 0.0014 | 0.0053 |
Endosome | 0.0079 | 0.0057 | 0.0053 | 0.0027 | 0.0029 | 0.0034 | 0.0272 | 0.0091 | 0.0091 | 0.0074 | 0.003 | 0.0092 | 0.0238 | 0.0037 | 0.0026 | 0.0051 | 0.005 | 0.0094 |
Golgi | 0.7168 | 0.8453 | 0.8459 | 0.9625 | 0.8926 | 0.9279 | 0.6879 | 0.874 | 0.842 | 0.8731 | 0.9211 | 0.9177 | 0.6148 | 0.7567 | 0.7387 | 0.4747 | 0.7721 | 0.8365 |
Lipid Particles | 0.0206 | 0.0034 | 0.0094 | 0.0038 | 0.0039 | 0.002 | 0.0224 | 0.0072 | 0.0039 | 0.0125 | 0.0127 | 0.003 | 0.016 | 0.0121 | 0.0043 | 0.003 | 0.0142 | 0.0035 |
Mitochondria | 0.009 | 0.0011 | 0.0015 | 0.0004 | 0.0004 | 0.0099 | 0.0266 | 0.0039 | 0.0028 | 0.0035 | 0.0004 | 0.0018 | 0.0371 | 0.0147 | 0.001 | 0.0016 | 0.0581 | 0.0044 |
None | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0.0001 | 0.008 | 0.001 | 0 | 0.0002 | 0.0069 | 0.0003 | 0 | 0 | 0.0013 | 0.0001 |
Nuclear Periphery | 0.0005 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0006 | 0.0041 | 0.0009 | 0.0027 | 0.0015 | 0.0002 | 0.0005 | 0.0095 | 0.0028 | 0.0002 | 0.0001 | 0.0002 | 0.0009 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0011 | 0 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0.0057 | 0 | 0 | 0.0029 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0009 | 0.0002 | 0 | 0.0003 | 0.0013 | 0.0105 | 0 | 0 | 0.0006 | 0.0001 |
Peroxisomes | 0.0004 | 0.0006 | 0 | 0 | 0 | 0 | 0.0017 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0034 | 0.0004 | 0.0001 | 0.0001 | 0.0103 | 0.0004 |
Punctate Nuclear | 0 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0214 | 0.0012 | 0 | 0 | 0.0004 | 0 |
Vacuole | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0017 | 0.0003 | 0.0021 | 0.0003 | 0 | 0.0002 | 0.0013 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0002 |
Vacuole Periphery | 0.0012 | 0.0007 | 0.0013 | 0.0004 | 0.0002 | 0.0013 | 0.0025 | 0.0006 | 0.0011 | 0.0009 | 0.0003 | 0.0005 | 0.0019 | 0.0007 | 0.0002 | 0.0003 | 0.001 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 147.9578 | 142.7174 | 113.3712 | 136.6231 | 129.8855 | 120.3205 | 133.3418 | 116.4146 | 115.973 | 149.8794 |
Translational Efficiency | 1.3922 | 1.3061 | 1.3091 | 1.0282 | 1.2094 | 1.5319 | 1.1107 | 1.0361 | 1.2065 | 1.1244 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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