Standard name
Human Ortholog
Description D-Tyr-tRNA(Tyr) deacylase; functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Cytoplasm 0.89 0.89 0.91 0.92 0.85 0.82 0.78 – 0.65 0.65 0.62 0.54 0.98 0.98 0.97 0.96 0.96 0.94 0.73 0.68 0.63 0.75 0.73 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0.08 0.08 0 0.06 0.05 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0 0.05 0 0.28 0.11 – 0.34 0.3 0.36 0.46 0 0 0 0 0 0 0.05 0.14 0.24 0.11 0.09 0.14
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0 0.16 – 0.14 0.06 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 0 2 0 0 – 0 1 0 1 3 1 2 0 1 0 2 2 1 1 8 15
Bud 0 4 0 2 1 6 1 – 1 5 6 9 1 1 1 0 0 0 1 0 0 0 6 3
Bud Neck 0 0 1 1 0 0 1 – 0 0 0 0 0 0 0 0 1 0 2 1 0 0 7 8
Bud Site 0 0 0 0 0 0 0 – 0 0 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 12 4 0 4 – 1 2 6 2 6 5 2 12 13 13 0 0 0 0 1 2
Cytoplasm 141 106 135 288 384 204 327 – 189 174 163 158 292 506 551 219 279 261 129 89 113 130 398 301
Endoplasmic Reticulum 2 1 3 0 2 1 1 – 0 1 1 2 3 2 3 9 11 10 2 0 1 1 8 5
Endosome 5 0 3 3 25 4 18 – 5 9 12 7 1 6 13 0 2 4 13 10 6 9 27 26
Golgi 2 1 0 1 2 0 0 – 0 0 0 0 0 1 0 0 0 1 3 0 0 0 1 0
Mitochondria 6 18 2 17 14 70 44 – 100 81 95 135 1 1 1 2 5 4 9 18 42 18 49 64
Nucleus 0 0 1 0 2 4 0 – 7 3 6 7 0 0 0 9 3 6 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 1 1 – 0 2 0 1 0 0 0 0 0 0 0 0 0 0 2 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 3 0 0 – 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 4 3 0 7 – 3 4 5 6 0 0 1 0 0 0 2 1 3 3 14 11
Vac/Vac Membrane 3 2 3 11 47 9 66 – 41 17 22 26 1 0 4 1 3 5 6 5 2 2 7 6
Unique Cell Count 159 119 148 312 453 249 417 290 269 263 291 297 515 567 229 290 279 178 132 180 173 546 457
Labelled Cell Count 161 132 154 339 489 299 470 347 301 320 355 308 524 578 252 318 305 178 132 180 173 546 457


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 4.9 5.8 6.3 6.8 4.7 5.7 – 5.1 5.1 5.2 4.8 6.6 6.5 6.7 6.8 7.3 7.3 5.4 5.5 5.6
Std Deviation (1e-4) 1.2 0.9 1.1 2.5 1.7 1.4 1.6 – 1.6 1.1 1.7 0.9 1.1 1.0 1.3 1.0 1.2 1.4 0.8 1.0 1.2
Intensity Change (Log2) – – – 0.12 0.22 -0.31 -0.02 – -0.18 -0.19 -0.16 -0.27 0.19 0.16 0.21 0.22 0.33 0.32 -0.1 -0.08 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0508 1.9716 1.41 1.5769 1.1102 1.4947 2.2204 3.7901 3.6271 2.9599 2.2022 3.5496 4.6159 6.2958 5.7842 4.8252 5.1372 5.368
Actin 0.011 0.0006 0.0082 0.0002 0.0273 0.0014 0.0122 0.0007 0.0073 0.0019 0.0017 0.0005 0.0328 0.0002 0.013 0.0823 0.0234 0.0025
Bud 0.0004 0.0002 0.0009 0.0006 0.0006 0.0002 0.0006 0.0005 0.0008 0.0011 0.0003 0.0006 0.0033 0.0001 0.001 0.0006 0.0002 0.001
Bud Neck 0.0024 0.0002 0.0009 0.0003 0.0025 0.0013 0.0023 0.0005 0.001 0.0009 0.0009 0.0032 0.0015 0.0001 0.0003 0.0005 0.0005 0.0008
Bud Periphery 0.0003 0.0001 0.0005 0.0001 0.0005 0.0001 0.0005 0.0002 0.0005 0.0006 0.0002 0.0007 0.0017 0 0.0009 0.0006 0.0003 0.0007
Bud Site 0.0017 0.0016 0.0048 0.0002 0.0028 0.0002 0.004 0.0037 0.0052 0.0006 0.0007 0.0005 0.0042 0.0005 0.001 0.0026 0.0003 0.0001
Cell Periphery 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.7297 0.8211 0.7914 0.8144 0.7603 0.8343 0.7978 0.8986 0.8945 0.9008 0.8673 0.8804 0.7357 0.9146 0.8392 0.7652 0.7908 0.8779
Cytoplasmic Foci 0.0431 0.0154 0.0351 0.0179 0.0621 0.0386 0.0301 0.0257 0.0254 0.019 0.0348 0.0262 0.0496 0.0081 0.0182 0.0205 0.0144 0.0225
Eisosomes 0.0003 0 0.0001 0 0.0003 0 0.0001 0 0.0004 0 0 0 0.0003 0 0.0001 0.0006 0.0011 0
Endoplasmic Reticulum 0.0112 0.0026 0.0096 0.014 0.0038 0.0049 0.0165 0.0038 0.0046 0.0142 0.006 0.005 0.0109 0.0033 0.0043 0.0014 0.0026 0.0084
Endosome 0.0353 0.0109 0.0365 0.0369 0.0451 0.0354 0.0559 0.0306 0.0215 0.0412 0.0523 0.0352 0.0304 0.0124 0.0102 0.0105 0.0138 0.0217
Golgi 0.005 0.0011 0.0067 0.001 0.0087 0.0031 0.0064 0.0044 0.0021 0.0021 0.0111 0.0017 0.0055 0.0006 0.0021 0.0065 0.004 0.0046
Lipid Particles 0.0025 0.0006 0.0013 0.0001 0.0198 0.0004 0.0021 0.0001 0.0014 0.0004 0.0006 0.0001 0.003 0.0002 0.0009 0.0048 0.0121 0.0023
Mitochondria 0.001 0.0006 0.0021 0.001 0.0022 0.0017 0.0018 0.0026 0.0019 0.0011 0.0072 0.0096 0.0026 0.0002 0.0009 0.0115 0.0034 0.0058
None 0.1336 0.1375 0.0944 0.1057 0.0472 0.0724 0.0425 0.0221 0.0238 0.0034 0.0053 0.0271 0.0878 0.0564 0.1006 0.0564 0.1032 0.0439
Nuclear Periphery 0.0045 0.0008 0.0014 0.0014 0.0014 0.0009 0.0068 0.0007 0.0012 0.0047 0.0025 0.001 0.0051 0.0004 0.0009 0.0007 0.0107 0.0024
Nucleolus 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.004 0 0.0001 0 0 0.0001 0.0003 0 0.0001 0.0001 0.0008 0.0001
Nucleus 0.0034 0.0025 0.0011 0.0012 0.0015 0.0016 0.0056 0.0012 0.0024 0.0027 0.0027 0.0029 0.0024 0.0007 0.001 0.0011 0.0018 0.0011
Peroxisomes 0.0043 0.0001 0.0011 0.0001 0.0081 0.0004 0.001 0.0003 0.0006 0.0003 0.0024 0.0004 0.0046 0 0.0033 0.0318 0.0116 0.0019
Punctate Nuclear 0.005 0.0007 0.001 0.0002 0.0013 0.0006 0.0016 0.0003 0.0011 0.0007 0.0006 0.0008 0.0153 0.0001 0.0005 0.0009 0.0026 0.0011
Vacuole 0.0041 0.0028 0.002 0.0032 0.0032 0.002 0.0063 0.0029 0.0034 0.0031 0.0022 0.0028 0.0021 0.0018 0.0009 0.0007 0.001 0.0009
Vacuole Periphery 0.0007 0.0004 0.0005 0.0012 0.0011 0.0006 0.0017 0.0007 0.0006 0.0008 0.001 0.0011 0.0006 0.0002 0.0002 0.0005 0.0014 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 63.3301 39.1938 37.1622 35.232 60.0036 63.295 34.1248 25.2608 35.6796 56.5905
Translational Efficiency 1.4342 1.6106 1.1212 1.651 1.3462 1.4333 2.0822 2.0151 1.6059 1.812

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1882 363 1628 157 1645 1937 266 1927 3527 2300 1894 2084

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 729.07 783.09 963.35 1014.67 746.68 827.54 1066.65 931.69 737.28 820.52 977.86 937.94
Standard Deviation 91.68 148.64 110.86 105.09 87.93 97.24 106.31 119.02 90.38 108.23 115.93 120.04
Intensity Change Log 2 — 0.103121 0.402003 0.476881 — 0.148339 0.514525 0.319360 — 0.126177 0.460031 0.399330

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000389 0.000834 0.000166 0.001322 0.000098 0.000239 0.000571 0.000745 0.000254 0.000333 0.000223 0.000789
Bud Neck 0.024926 0.022139 0.001974 0.003756 0.005167 0.046983 0.001880 0.013807 0.015710 0.043062 0.001960 0.013050
Bud Site 0.015945 0.040409 0.008878 0.020607 0.004339 0.024528 0.012282 0.037070 0.010532 0.027034 0.009356 0.035830
Cell Periphery 0.000162 0.000207 0.000080 0.000118 0.000110 0.000135 0.000194 0.000084 0.000138 0.000146 0.000096 0.000087
Cytoplasm 0.374887* 0.382649* 0.645946* 0.484569* 0.610533* 0.334701* 0.539250* 0.510774* 0.484793* 0.342269* 0.630961* 0.508800*
Cytoplasmic Foci 0.350902* 0.287279* 0.016200 0.074498 0.186868* 0.252175* 0.031761 0.096796 0.274396* 0.257715* 0.018385 0.095116
Eisosomes 0.000266 0.000111 0.000020 0.000063 0.000064 0.000106 0.000063 0.000035 0.000172 0.000107 0.000026 0.000037
Endoplasmic Reticulum 0.002106 0.001012 0.001735 0.002413 0.001181 0.001409 0.003960 0.000933 0.001675 0.001346 0.002048 0.001044
Endosome 0.029979 0.053776 0.002407 0.008140 0.009615 0.059903 0.007246 0.020163 0.020481 0.058936 0.003086 0.019258
Golgi 0.012516 0.012568 0.000117 0.002003 0.002444 0.011142 0.000195 0.003487 0.007819 0.011367 0.000128 0.003375
Lipid Particles 0.013358 0.005722 0.000861 0.002168 0.005104 0.006984 0.002534 0.001838 0.009508 0.006785 0.001096 0.001863
Mitochondria 0.017051 0.006833 0.000446 0.003768 0.002809 0.017969 0.000269 0.001674 0.010408 0.016212 0.000422 0.001832
Mitotic Spindle 0.002496 0.002737 0.010659 0.010378 0.001078 0.005352 0.008136 0.036495 0.001835 0.004939 0.010305 0.034527
None 0.001666 0.004445 0.003619 0.002860 0.003528 0.002202 0.002369 0.001538 0.002534 0.002556 0.003443 0.001637
Nuclear Periphery 0.000283 0.000212 0.001151 0.001718 0.000432 0.000411 0.001130 0.000601 0.000352 0.000379 0.001148 0.000685
Nuclear Periphery Foci 0.000368 0.001317 0.001992 0.005784 0.000535 0.000333 0.010222 0.001215 0.000446 0.000488 0.003148 0.001560
Nucleolus 0.001259 0.004491 0.000215 0.000230 0.001074 0.002396 0.000208 0.001183 0.001173 0.002727 0.000214 0.001111
Nucleus 0.065648 0.035318 0.227793* 0.064521 0.123899 0.064912 0.170397* 0.052175 0.092817 0.060242 0.219732* 0.053105
Peroxisomes 0.008947 0.019753 0.000396 0.003123 0.001902 0.010416 0.000478 0.006869 0.005661 0.011890 0.000408 0.006586
Vacuole 0.072904 0.112819 0.075114 0.304261* 0.037511 0.149382 0.206566* 0.209335* 0.056397 0.143612 0.093576 0.216487*
Vacuole Periphery 0.003941 0.005371 0.000231 0.003701 0.001710 0.008319 0.000289 0.003181 0.002900 0.007854 0.000239 0.003220

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.04 1.63 -2.22 -0.50 -2.73 -4.25 -8.53 -4.27 -3.00 0.05 -1.66 -0.98 -3.28 -2.60 -2.69
Bud Neck 1.11 17.52 15.33 6.54 -2.62 -17.80 5.26 -8.04 13.94 -12.40 -12.85 17.74 3.25 14.41 -12.63
Bud Site -5.78 4.78 1.28 5.96 -1.99 -12.75 -2.38 -12.88 -1.48 -4.96 -10.47 1.84 -9.17 0.08 -9.35
Cell Periphery -1.08 8.01 4.64 2.61 -1.78 -2.02 -4.55 6.46 5.74 7.05 -0.60 5.91 9.76 6.45 4.72
Cytoplasm -0.45 -21.80 0.45 0.65 8.59 31.21 9.72 13.69 -16.47 -2.38 18.98 -8.74 0.96 -15.55 8.50
Cytoplasmic Foci 5.47 62.63 35.55 18.27 -5.66 -9.56 30.89 19.64 30.32 -14.98 2.93 66.02 42.27 34.33 -22.10
Eisosomes 5.75 9.50 7.83 4.25 -7.64 -4.97 -0.06 9.57 9.27 6.94 4.24 10.24 10.03 10.45 -1.80
Endoplasmic Reticulum 4.07 -1.22 -1.38 -5.28 -0.60 -0.90 -8.68 2.04 2.30 9.95 1.46 -5.65 4.23 1.66 13.29
Endosome -4.97 20.19 16.20 10.58 -4.00 -21.45 4.60 -4.81 18.50 -8.67 -17.60 21.55 5.39 20.34 -13.33
Golgi 0.15 14.05 11.77 5.21 -1.22 -10.24 5.87 0.42 10.10 -4.36 -3.92 15.06 8.60 10.99 -4.57
Lipid Particles 5.96 13.02 11.18 3.79 -5.31 -2.86 5.99 7.73 9.65 2.42 3.79 15.20 13.69 10.38 -2.95
Mitochondria 4.84 10.89 8.91 3.03 -0.93 -11.54 5.34 2.74 13.40 -7.41 -4.23 11.72 10.38 13.75 -6.08
Mitotic Spindle -0.27 -4.37 -1.79 -1.72 0.08 -4.54 -1.38 -10.89 -8.94 -5.78 -3.64 -5.05 -10.71 -9.22 -6.58
None -2.48 -5.74 -0.50 2.06 2.49 2.99 1.67 6.50 1.80 2.88 -0.03 -2.76 4.41 2.69 7.24
Nuclear Periphery 1.50 -20.10 -5.43 -5.65 -0.25 0.27 -10.07 -5.62 -6.92 6.48 -0.54 -19.68 -10.29 -8.89 9.44
Nuclear Periphery Foci -5.36 -12.61 -7.56 -5.52 -3.94 3.24 -10.59 -4.72 -6.33 9.83 -1.26 -15.50 -7.95 -7.01 9.11
Nucleolus -1.97 5.00 4.65 2.57 -0.87 -3.96 4.60 -0.65 3.16 -4.49 -4.14 6.71 -0.04 3.79 -4.43
Nucleus 6.25 -31.82 -2.71 -5.74 17.11 14.01 -6.97 14.73 0.03 12.09 10.67 -28.75 8.88 -2.00 33.30
Peroxisomes -4.10 13.20 8.99 7.00 -2.71 -13.97 7.11 -6.31 7.42 -10.42 -8.64 14.20 1.52 9.40 -10.39
Vacuole -5.26 -9.77 -15.24 -12.75 -13.08 -25.26 -15.26 -38.61 -16.12 0.68 -21.72 -18.33 -37.74 -19.48 -18.45
Vacuole Periphery -1.61 13.93 2.61 3.18 -0.97 -11.49 7.31 -0.70 8.81 -3.99 -9.87 15.43 1.94 9.11 -4.12
Standard name
Human Ortholog
Description D-Tyr-tRNA(Tyr) deacylase; functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes
Localization
Cell Percentages cytoplasm (67%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dtd1

Dtd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dtd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available