Standard name
Human Ortholog
Description Component of the septin ring that is required for cytokinesis; present at the ends of rod-like septin hetero-oligomers; C-terminal extension is important for recruitment of Bni5p to the mother-bud neck, which in turn is required for Myo1p recruitment and cytokinesis; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.13 0.15 0.11 0.12 0.1 0.1 0.12 0.12 0.19 0.14 0.13 0.08 0.16 0.12 0.05 0.1 0.09 0.09 0.08 0.1 0.14 0.28 0.2 0.22
Bud 0.07 0.08 0.07 0.07 0.11 0.12 0.12 0.16 0.15 0.14 0.14 0.12 0.06 0.06 0 0 0 0 0.35 0.33 0.31 0.33 0.32 0.23
Bud Neck 0.3 0.34 0.22 0.29 0.35 0.21 0.24 0.15 0.19 0.23 0.23 0.25 0.3 0.24 0.17 0.19 0.16 0.14 0.44 0.43 0.38 0.27 0.33 0.37
Bud Site 0.27 0.2 0.22 0.27 0.16 0.24 0.14 0.11 0.17 0.16 0.14 0.12 0.13 0.1 0.05 0.09 0 0.07 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.1 0 0.05 0 0 0 0 0 0 0.07
Cytoplasm 0.12 0.11 0.11 0 0.07 0.07 0.09 0.06 0.07 0 0.09 0.08 0.08 0.15 0.21 0.2 0.2 0.25 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.09 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.15 0.17 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.09 0.11 0.12 0.18 0.22 0.24 0.29 0 0 0 0.07 0.09 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0.05 0.05 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.29 0.15 0.15 0.2 0.19 0.21 0.26 0.36 0.23 0.26 0.23 0.25 0.15 0.17 0.18 0.16 0.19 0.22 0 0 0 0 0 0
Peroxisomes 0 0 0.13 0.11 0.12 0.16 0.13 0.09 0.12 0.1 0.09 0.07 0.12 0.11 0.16 0.05 0.06 0 0 0 0 0 0 0
SpindlePole 0 0.06 0.07 0.07 0.11 0.1 0.11 0.09 0.08 0.09 0.05 0.06 0.1 0.11 0.12 0.24 0.16 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 22 27 44 21 32 45 58 63 76 68 24 19 46 36 16 22 9 9 18 24 83 107 121 129
Bud 12 14 30 12 36 53 57 85 59 69 25 27 17 18 7 5 2 2 77 83 176 125 192 139
Bud Neck 50 59 88 53 109 92 114 81 73 115 42 59 90 72 52 44 17 15 96 110 219 102 198 220
Bud Site 45 35 90 48 50 106 65 61 66 79 26 28 39 31 16 20 2 7 – – – – – –
Cell Periphery 3 5 12 8 3 17 14 13 20 15 7 9 11 14 31 4 5 1 1 1 4 6 17 40
Cytoplasm 20 19 44 8 22 30 41 33 27 20 16 19 25 46 64 47 21 26 4 5 8 5 3 5
Endoplasmic Reticulum 3 1 9 3 2 7 2 4 11 9 5 5 2 2 6 2 1 5 0 2 9 0 4 10
Endosome 5 7 11 7 11 12 21 23 7 12 3 5 14 22 28 6 2 2 2 4 7 6 9 7
Golgi 2 0 3 1 1 0 2 2 1 0 1 2 2 3 2 26 16 18 0 2 4 3 3 3
Mitochondria 9 3 8 8 14 38 51 63 71 109 45 67 1 1 2 16 9 8 2 2 5 7 15 20
Nucleus 5 5 17 9 17 20 16 23 13 26 6 10 9 12 9 8 1 3 0 3 1 0 0 0
Nuclear Periphery 0 0 1 0 1 0 1 1 1 4 1 1 0 0 1 0 0 0 1 0 2 2 6 1
Nucleolus 48 26 59 36 60 92 125 190 91 127 43 59 45 51 55 37 20 23 3 3 8 0 0 0
Peroxisomes 5 7 54 19 39 70 61 46 48 49 17 16 36 34 49 11 6 4 2 1 11 5 15 11
SpindlePole 3 11 30 12 35 42 55 49 33 45 9 13 30 34 36 56 17 11 5 4 23 2 3 3
Vac/Vac Membrane 1 1 14 3 5 3 7 12 4 1 1 3 5 9 7 5 5 1 0 1 2 1 0 2
Unique Cell Count 167 176 402 180 315 436 480 533 394 494 185 234 296 299 302 231 104 104 220 256 578 383 596 602
Labelled Cell Count 233 220 514 248 437 627 690 749 601 748 271 342 372 385 381 309 133 135 220 256 578 383 596 602


Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.3 14.0 13.1 11.6 11.0 9.9 9.2 8.9 9.2 8.3 8.6 8.1 13.6 13.5 13.4 17.6 21.9 20.2 11.0 11.6 12.3
Std Deviation (1e-4) 3.7 3.2 2.9 2.3 3.9 3.1 2.9 2.7 2.7 2.5 2.3 2.5 3.2 4.1 4.0 5.6 8.7 7.9 1.8 2.1 2.2
Intensity Change (Log2) – – – -0.18 -0.24 -0.39 -0.5 -0.55 -0.51 -0.66 -0.6 -0.68 0.06 0.04 0.04 0.43 0.75 0.63 -0.24 -0.17 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 -0.3 -0.3 0.5 0.4 3.3 1.3 0.7 -1.2 1.8 0.5 -2.7 -0.6 -0.7 -0.7
Bud -0.3 1.8 2.3 2.2 3.9 3.4 3.1 2.3 1.7 -0.9 -0.7 -3.0 -2.8 -2.1 -2.1
Bud Neck 2.0 3.8 -0.3 0.7 -2.6 -1.2 0.5 0.2 1.0 2.6 0.7 -1.5 -0.8 -1.2 -1.7
Bud Site 1.1 -2.2 0.7 -3.4 -4.5 -2.0 -2.4 -2.4 -3.3 -3.1 -4.2 -6.3 -4.4 -4.8 -3.6
Cell Periphery 0.9 -1.9 0.7 -0.1 -0.5 1.5 0 0.5 0.6 0.5 1.2 4.0 -1.0 0 0
Cytoplasm -2.5 -1.8 -2.1 -1.2 -2.6 -2.0 -4.0 -0.9 -1.2 -1.1 1.7 3.7 3.2 2.5 3.7
Endoplasmic Reticulum 0 0 -0.7 -2.4 -1.9 0.5 -0.4 0 -0.1 0 0 -0.2 0 0 0
Endosome 0.7 0.6 0 1.3 1.3 -0.9 -0.3 0 -0.5 1.4 2.9 3.8 -0.1 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 6.1 0 0
Mitochondria 0 1.9 4.3 5.1 5.6 7.6 8.9 8.8 10.0 0 0 0 3.1 0 0
Nucleus 0.4 0.7 0.3 -0.7 0.1 -0.7 0.7 -0.6 0 -0.8 -0.1 -0.9 -0.5 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.6 1.6 2.4 4.1 7.2 3.0 4.0 2.5 3.3 0.2 0.9 1.3 0.5 1.1 1.8
Peroxisomes -1.0 -0.4 1.1 -0.3 -2.4 -0.5 -1.6 -1.5 -2.6 -0.5 -0.8 1.0 -3.5 -2.2 -2.7
SpindlePole -0.3 1.7 1.1 2.0 0.9 0.5 0.9 -1.2 -0.9 1.2 1.8 2.0 5.9 2.8 1.0
Vacuole -1.2 -1.6 -2.9 -2.0 -1.1 -2.3 -3.8 0 -1.7 -1.4 -0.3 -0.9 -0.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.8028 9.443 10.4046 9.7159 8.1196 9.6973 10.346 11.6112 11.8572 10.4677 10.0636 12.2293 10.1627 11.9972 12.5869 13.3027 12.5925 12.5714
Actin 0.0977 0.0261 0.0263 0.0401 0.0118 0.1743 0.1359 0.0434 0.0447 0.0089 0.0487 0.1785 0.1424 0.0849 0.0459 0.0002 0.0633 0.0709
Bud 0.0156 0.0135 0.0054 0.001 0.0018 0.0047 0.0085 0.0177 0.0103 0.0309 0.0056 0.0033 0.0081 0.0093 0.0058 0.0003 0.0011 0.0045
Bud Neck 0.1542 0.0288 0.6315 0.8356 0.8034 0.7587 0.1637 0.0434 0.5114 0.6712 0.6667 0.7791 0.2201 0.0644 0.6587 0.9814 0.8293 0.8905
Bud Periphery 0.0158 0.0121 0.0055 0.0013 0.0029 0.0108 0.0079 0.0142 0.0107 0.0552 0.0062 0.0091 0.0067 0.0115 0.0094 0.0003 0.0016 0.0132
Bud Site 0.4893 0.839 0.2697 0.0528 0.0184 0.0177 0.4196 0.7857 0.3232 0.0579 0.0704 0.0072 0.3907 0.6651 0.2204 0.002 0.0042 0.0096
Cell Periphery 0.0038 0.0039 0.0036 0.0014 0.0021 0.0025 0.003 0.004 0.0052 0.0034 0.002 0.0019 0.0025 0.0105 0.0056 0.001 0.0018 0.0027
Cytoplasm 0.0054 0.0132 0.0008 0.0003 0.0016 0.0009 0.0108 0.0584 0.0009 0.0015 0.0031 0.0009 0.0071 0.0004 0.0021 0.0001 0.0002 0.0009
Cytoplasmic Foci 0.0927 0.0164 0.0138 0.0049 0.0193 0.0089 0.0322 0.002 0.0173 0.0084 0.0691 0.0038 0.091 0.0006 0.0039 0.0019 0.0046 0.0015
Eisosomes 0.003 0.0002 0.0004 0.0017 0.0008 0.0005 0.0018 0.0005 0.0003 0.0001 0.0005 0.0005 0.0011 0.0004 0.0002 0 0.0004 0.0002
Endoplasmic Reticulum 0.0037 0.0002 0.0002 0.0001 0.0027 0.0001 0.0079 0.0004 0.0002 0.0009 0.001 0.0001 0.0012 0.0001 0.0004 0 0 0.0002
Endosome 0.0127 0.0012 0.0014 0.0028 0.0202 0.0006 0.0203 0.0005 0.0091 0.0432 0.0247 0.001 0.0131 0.0008 0.0189 0.0001 0.0002 0.0008
Golgi 0.0064 0.0003 0.0018 0.006 0.0121 0.0018 0.0266 0.0005 0.0128 0.033 0.0344 0.0028 0.0038 0.0003 0.0034 0.0002 0.0012 0.0015
Lipid Particles 0.0092 0.001 0.0073 0.0062 0.0137 0.0027 0.0291 0.0004 0.003 0.0039 0.0103 0.0053 0.0075 0.0006 0.001 0.0005 0.0008 0.0003
Mitochondria 0.0066 0.0009 0.0023 0.0147 0.0369 0.0014 0.0099 0.002 0.0189 0.0657 0.0042 0.0014 0.0091 0.0015 0.001 0.0001 0.001 0.0002
None 0.0129 0.0002 0.0065 0.0009 0.0041 0.0001 0.0371 0.0003 0.0003 0.0002 0.0022 0.0001 0.0091 0.0001 0.0002 0 0 0
Nuclear Periphery 0.0061 0.0012 0.0002 0.0001 0.0047 0.0001 0.0189 0.0032 0.0001 0.0003 0.0004 0.0001 0.0259 0.0001 0.0004 0 0 0.0001
Nucleolus 0.0072 0.0194 0.0111 0.0079 0.0091 0.0029 0.0074 0.0029 0.0086 0.0026 0.0135 0.0013 0.0171 0.0968 0.0074 0.0094 0.0844 0.0005
Nucleus 0.0063 0.0072 0.0017 0.0016 0.0027 0.0011 0.0117 0.0148 0.0013 0.0016 0.0046 0.0003 0.0049 0.0249 0.0027 0.0015 0.0039 0.0005
Peroxisomes 0.0303 0.0021 0.0061 0.0193 0.0212 0.0064 0.0128 0.0015 0.0195 0.002 0.0082 0.0023 0.0213 0.0007 0.0026 0.0003 0.0008 0.0004
Punctate Nuclear 0.0185 0.0011 0.0036 0.0007 0.003 0.0009 0.0286 0.0005 0.0006 0.0004 0.0219 0.0003 0.0047 0.0004 0.0005 0.0003 0.0007 0.0003
Vacuole 0.0018 0.0113 0.0009 0.0005 0.0033 0.0026 0.0048 0.0023 0.0013 0.0068 0.0018 0.0006 0.0103 0.0229 0.0081 0.0003 0.0003 0.0012
Vacuole Periphery 0.0008 0.0008 0.0001 0.0002 0.0041 0.0004 0.0016 0.0014 0.0002 0.0017 0.0005 0.0001 0.0025 0.0036 0.0014 0 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.317 30.3775 25.1934 34.7198 27.6447 26.6375 33.8765 31.502 31.2962 34.5386
Translational Efficiency 2.0533 1.9915 1.9467 1.5394 1.8785 2.1281 1.6588 1.4765 1.6104 1.6431

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the septin ring that is required for cytokinesis; present at the ends of rod-like septin hetero-oligomers; C-terminal extension is important for recruitment of Bni5p to the mother-bud neck, which in turn is required for Myo1p recruitment and cytokinesis; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Shs1

Shs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Shs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available