Standard name
Human Ortholog
Description Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.08 0.08 0.11 0.1 0.11 0.13 0.15 – 0.14 0.2 0.17 0.08 0.07 0.05 0.05 0.25 0.12 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.12 0.09 0.07 0 0.09 0.06 0.08 – 0.12 0.16 0.15 0.05 0.11 0.15 0.74 0.19 0.54 0 0 0.05 0 0 0
Cytoplasm 0.05 0.08 0.08 0.15 0.18 0.12 0.11 0.11 – 0.13 0.09 0.1 0 0.06 0.06 0 0.12 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0.89 0.84 0.87 0.6 0.74 0.71 0.71 0.7 – 0.59 0.57 0.6 0.93 0.88 0.89 0 0 0 0.95 0.89 0.88 0.86 0.84 0.82
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.1 0 0 0.27 0.14 0 0.05 0 – 0.05 0 0 0.1 0.07 0 0.16 0.5 0.12 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.16 0.07 0.12 0.23 0.18 – 0.26 0.38 0.37 0 0 0 0.16 0.12 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 24 16 16 9 16 23 34 40 – 40 49 36 24 33 22 1 4 7 6 5 8 4 12 9
Bud 3 0 2 2 1 1 5 6 – 4 6 5 0 3 1 0 0 0 1 0 0 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 13 23 18 6 6 19 17 22 – 35 40 33 14 51 67 14 3 30 4 3 8 5 13 13
Cytoplasm 21 15 16 12 28 24 30 30 – 38 22 22 11 28 26 0 2 10 1 5 1 2 6 8
Endoplasmic Reticulum 343 164 166 49 115 144 188 192 – 171 142 128 276 409 388 0 0 2 378 188 168 184 307 330
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1
Golgi 40 4 5 22 21 8 12 8 – 15 10 5 29 33 10 3 8 7 2 1 0 7 12 15
Mitochondria 1 0 9 13 11 24 60 49 – 76 95 80 0 1 0 3 2 7 0 2 1 3 6 10
Nucleus 0 0 0 0 1 0 2 1 – 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 1 1 1 2 7 8 – 5 9 4 7 3 1 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 8 0 0 2 1 12 7 3 – 10 5 5 5 7 2 0 0 1 0 3 1 0 5 6
Unique Cell Count 387 195 191 82 155 203 263 273 292 250 214 297 466 437 19 16 56 398 213 192 215 367 405
Labelled Cell Count 454 222 233 116 202 257 362 359 394 380 318 366 569 518 21 19 64 398 213 192 215 367 405


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.7 35.4 23.2 21.0 20.0 19.0 17.2 17.5 – 16.1 16.8 16.5 29.9 30.2 31.0 38.5 35.6 36.5 32.8 33.2 33.6
Std Deviation (1e-4) 6.7 7.0 4.9 6.0 5.4 4.2 4.1 4.2 – 4.2 3.8 4.0 5.5 6.5 5.8 6.6 7.4 6.8 7.0 6.1 7.6
Intensity Change (Log2) – – – -0.14 -0.21 -0.29 -0.43 -0.4 – -0.52 -0.47 -0.49 0.37 0.38 0.42 0.73 0.62 0.66 0.5 0.52 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.7 0.6 1.0 1.5 2.0 – 1.8 3.3 2.5 -0.1 -0.6 -1.6 0 0 0.9
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -0.6 -2.0 0 -1.2 -0.5 – 0.9 2.0 1.8 -2.1 0.6 2.0 0 0 7.3
Cytoplasm 1.6 2.7 1.1 1.1 0.9 – 1.6 0.2 0.7 -2.2 -1.1 -1.1 0 0 2.0
Endoplasmic Reticulum -5.0 -3.0 -3.9 -3.9 -4.2 – -6.6 -6.8 -6.1 2.2 0.3 0.7 -8.9 -8.4 -11.8
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 6.1 3.8 0.7 1.1 0.2 – 1.4 0.8 0 3.0 2.2 0 0 0 0
Mitochondria 3.1 0.9 2.5 5.3 4.2 – 6.0 8.2 7.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.4 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 30.2297 27.6032 28.23 28.4996 25.9815 28.0668 32.3887 33.0286 31.2315 30.77 29.1484 31.1961 25.7937 29.0913 25.3839 25.5799 25.7643 27.6751
Actin 0.0026 0.0026 0.0021 0 0.0001 0.0004 0.0005 0.0004 0.0001 0 0.0009 0.0006 0.0448 0.0021 0.0119 0 0 0.0003
Bud 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0006 0.0001 0.0005 0 0 0
Bud Neck 0.0004 0.0002 0.0024 0.0001 0.0004 0.0007 0.0002 0.0001 0.0001 0 0.0005 0.0002 0.0007 0.0026 0.0004 0.0001 0 0.0002
Bud Periphery 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0002 0.0001 0 0.0007 0.0002 0.001 0.0004 0.0006 0 0 0
Bud Site 0.0002 0.0001 0 0 0 0 0 0.0002 0 0 0 0 0.0019 0.0016 0.0044 0 0 0
Cell Periphery 0.0096 0.0174 0.0167 0.0061 0.0121 0.0054 0.0101 0.0165 0.0058 0.0038 0.0101 0.004 0.0104 0.0449 0.0077 0.0013 0.0015 0.0029
Cytoplasm 0.0071 0.0117 0.0099 0.0007 0.0025 0.0083 0.0017 0.002 0.0004 0.0001 0.0009 0.0005 0.0068 0.0114 0.0028 0.0004 0.0003 0.0019
Cytoplasmic Foci 0.0008 0.0008 0.0002 0 0.0002 0.0002 0 0.0001 0 0 0.0001 0 0.0036 0.0006 0.0113 0 0 0.0009
Eisosomes 0.0006 0.0011 0.0023 0.0004 0.0002 0.0002 0.0008 0.0013 0.0003 0 0.0024 0.0013 0.0084 0.0027 0.0003 0 0 0.0001
Endoplasmic Reticulum 0.9231 0.9271 0.9477 0.9869 0.9611 0.9703 0.9595 0.9583 0.9843 0.9947 0.969 0.9782 0.7664 0.883 0.9091 0.9899 0.9946 0.9552
Endosome 0.0025 0.0027 0.0007 0.0003 0.0028 0.001 0.0005 0.0011 0.0005 0 0.0013 0.0007 0.0227 0.0013 0.0041 0.0004 0.0001 0.0211
Golgi 0.0026 0.01 0.0063 0.0011 0.0038 0.0029 0.0016 0.0066 0.0017 0.0001 0.0091 0.007 0.0143 0.0076 0.0043 0.0004 0.0007 0.0048
Lipid Particles 0.0062 0.0162 0.0026 0.0003 0.003 0.0017 0.0012 0.0014 0.0007 0 0.0003 0.0007 0.0079 0.0254 0.0031 0.0001 0.0003 0.0007
Mitochondria 0.0007 0.0004 0.0001 0 0.0022 0.0001 0.0002 0.001 0.0001 0 0.0002 0.0005 0.0059 0.0005 0.0244 0.0001 0 0.0008
None 0.0006 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0017 0.0002 0.0003 0 0 0.0001
Nuclear Periphery 0.0301 0.0068 0.0063 0.0032 0.0041 0.0067 0.0196 0.0079 0.0046 0.0012 0.0033 0.0044 0.0722 0.0075 0.0058 0.0061 0.0018 0.0091
Nucleolus 0.0006 0 0 0 0 0 0 0 0 0 0 0 0.0002 0.0001 0.0001 0 0 0
Nucleus 0.0021 0.0002 0.0002 0.0001 0.0001 0.0002 0.0003 0.0003 0 0 0.0001 0.0001 0.0055 0.0004 0.0003 0.0001 0 0.0001
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0008 0 0.0062 0 0 0
Punctate Nuclear 0.0035 0 0 0 0 0 0 0 0 0 0 0 0.0139 0.0001 0.0005 0 0 0
Vacuole 0.0046 0.0024 0.0019 0.0006 0.0046 0.0014 0.0018 0.0018 0.0007 0.0001 0.0007 0.0009 0.0065 0.0072 0.0016 0.0008 0.0005 0.0009
Vacuole Periphery 0.0016 0.0003 0.0002 0.0001 0.0022 0.0002 0.0017 0.0005 0.0003 0 0.0003 0.0005 0.0038 0.0003 0.0004 0.0004 0.0001 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 159.0493 195.498 125.2859 124.5837 141.7724 119.2209 166.2207 133.4124 118.7049 136.8791
Translational Efficiency 1.1162 0.7452 0.6335 0.5938 0.9438 1.7434 0.7702 0.7134 0.9039 1.4736

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1915 1797 1903 2361 1642 1888 2051 1634 3557 3685 3954 3995

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1834.16 2353.96 2805.44 2567.30 1892.86 2074.42 2875.36 2827.75 1861.26 2210.74 2841.71 2673.83
Standard Deviation 348.04 457.10 447.67 400.06 376.48 424.48 425.01 521.63 362.63 462.31 437.46 471.46
Intensity Change Log 2 — 0.359970 0.613108 0.485132 — 0.132140 0.603175 0.579087 — 0.248756 0.608072 0.533614

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000887 0.001464 0.000941 0.000766 0.002749 0.001956 0.000272 0.000745 0.001747 0.001716 0.000594 0.000757
Bud Neck 0.000218 0.000233 0.001624 0.000730 0.000219 0.000234 0.000340 0.000577 0.000219 0.000234 0.000958 0.000668
Bud Site 0.001168 0.001113 0.000945 0.002861 0.000804 0.002358 0.000276 0.001361 0.001000 0.001751 0.000598 0.002247
Cell Periphery 0.004619 0.003054 0.003949 0.004703 0.005310 0.002619 0.003486 0.014991 0.004938 0.002831 0.003709 0.008911
Cytoplasm 0.000268 0.000153 0.000594 0.002147 0.000301 0.000939 0.000491 0.000550 0.000283 0.000555 0.000541 0.001494
Cytoplasmic Foci 0.001504 0.000527 0.000221 0.001140 0.000925 0.002315 0.000327 0.000879 0.001237 0.001443 0.000276 0.001033
Eisosomes 0.000819 0.000901 0.000284 0.000185 0.001197 0.000639 0.000433 0.000783 0.000994 0.000766 0.000362 0.000430
Endoplasmic Reticulum 0.958226* 0.970585* 0.946025* 0.938450* 0.951117* 0.954706* 0.972949* 0.932284* 0.954945* 0.962449* 0.959991* 0.935928*
Endosome 0.005855 0.004262 0.008008 0.007932 0.005132 0.007785 0.003233 0.005326 0.005521 0.006067 0.005531 0.006866
Golgi 0.012109 0.008584 0.006371 0.007315 0.016661 0.012022 0.006246 0.013817 0.014210 0.010345 0.006306 0.009975
Lipid Particles 0.004329 0.004482 0.013112 0.017154 0.004307 0.007820 0.006359 0.013146 0.004319 0.006192 0.009609 0.015515
Mitochondria 0.003064 0.001257 0.000450 0.000519 0.004002 0.000430 0.000164 0.001115 0.003497 0.000833 0.000302 0.000762
Mitotic Spindle 0.000071 0.000029 0.000064 0.000142 0.000099 0.000369 0.000018 0.000435 0.000084 0.000203 0.000040 0.000262
None 0.000699 0.000496 0.000363 0.000713 0.001349 0.000520 0.000529 0.000472 0.000999 0.000508 0.000449 0.000614
Nuclear Periphery 0.000611 0.000509 0.004467 0.001661 0.000823 0.000462 0.001144 0.001846 0.000709 0.000485 0.002743 0.001737
Nuclear Periphery Foci 0.001094 0.000362 0.004804 0.004008 0.000519 0.001251 0.000684 0.001255 0.000829 0.000818 0.002667 0.002882
Nucleolus 0.000008 0.000003 0.000009 0.000006 0.000028 0.000004 0.000002 0.000021 0.000018 0.000004 0.000005 0.000013
Nucleus 0.000054 0.000025 0.000068 0.000124 0.000133 0.000073 0.000028 0.000087 0.000091 0.000050 0.000047 0.000109
Peroxisomes 0.000207 0.000088 0.000016 0.000130 0.000214 0.000257 0.000097 0.000235 0.000210 0.000175 0.000058 0.000173
Vacuole 0.001543 0.001021 0.006024 0.007778 0.001433 0.001859 0.002239 0.007138 0.001493 0.001450 0.004061 0.007516
Vacuole Periphery 0.002644 0.000852 0.001660 0.001537 0.002677 0.001381 0.000681 0.002937 0.002659 0.001123 0.001152 0.002110

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.12 -0.66 0.19 1.26 0.84 0.76 2.94 2.23 1.94 -1.61 0.03 2.39 2.17 2.33 -0.47
Bud Neck -0.18 -4.66 -4.64 -4.41 2.65 -0.42 -0.84 -2.30 -2.17 -1.10 -0.37 -4.46 -5.00 -4.80 1.37
Bud Site 0.12 0.42 -2.98 -2.93 -3.43 -2.62 2.86 -1.84 1.55 -3.80 -1.99 1.76 -3.50 -1.26 -4.69
Cell Periphery 2.29 0.92 -0.25 -2.75 -1.29 4.77 2.81 -7.40 -9.98 -9.01 4.77 2.59 -6.11 -9.91 -8.29
Cytoplasm 1.35 -2.24 -4.75 -5.10 -3.63 -2.37 -0.84 -1.37 1.40 -0.26 -1.91 -2.03 -4.92 -3.54 -3.55
Cytoplasmic Foci 3.29 4.68 1.03 -2.58 -4.37 -2.94 3.21 0.11 2.64 -1.74 -0.73 5.55 0.82 1.35 -4.12
Eisosomes -0.15 1.44 1.72 1.86 1.45 1.36 2.47 1.07 -0.36 -1.21 0.67 2.56 2.16 1.27 -0.52
Endoplasmic Reticulum -3.84 3.53 5.43 8.82 1.89 -0.72 -5.96 4.05 4.70 9.74 -2.71 -1.40 6.64 9.06 7.97
Endosome 3.73 -4.23 -4.03 -6.85 -0.04 -4.57 4.47 -0.49 4.19 -5.11 -1.53 -0.28 -3.80 -2.21 -3.48
Golgi 3.29 5.14 3.76 1.14 -0.93 2.79 7.69 1.58 -1.10 -5.66 3.94 9.18 3.98 0.33 -4.48
Lipid Particles -0.20 -5.92 -7.93 -7.70 -1.98 -3.26 -2.33 -7.05 -3.52 -4.66 -2.79 -6.19 -10.31 -7.96 -4.49
Mitochondria 1.86 3.01 3.00 1.39 -0.03 3.17 3.42 2.45 -2.08 -3.00 3.66 4.59 3.87 0.16 -2.72
Mitotic Spindle 3.80 0.30 -1.54 -2.35 -1.61 -1.39 2.88 -1.37 0.08 -1.70 -1.25 2.78 -1.76 -0.11 -2.16
None 0.80 1.58 -0.14 -1.06 -2.13 1.65 1.57 1.71 0.29 0.25 1.83 2.04 1.36 -0.83 -1.22
Nuclear Periphery 2.26 -8.42 -6.88 -7.55 5.74 3.13 -2.89 -8.97 -10.09 -5.01 3.61 -8.89 -9.97 -11.30 4.01
Nuclear Periphery Foci 2.28 -5.91 -5.66 -8.25 0.68 -2.32 -1.65 -3.41 -0.10 -2.33 0.04 -5.83 -6.58 -6.75 -0.95
Nucleolus 3.88 -0.37 0.90 -4.11 1.01 1.36 1.49 0.35 -2.20 -2.49 1.70 1.46 0.52 -2.83 -2.19
Nucleus 1.81 -1.29 -1.46 -1.70 -1.14 0.78 1.50 0.61 -0.45 -3.40 1.11 0.85 -1.08 -1.67 -1.55
Peroxisomes 1.97 3.27 1.02 -0.79 -2.28 -0.38 1.54 -0.18 0.17 -1.30 0.54 3.15 0.59 0.04 -2.10
Vacuole 1.72 -6.64 -8.65 -9.50 -1.99 -1.09 -2.38 -6.82 -5.68 -5.16 -0.05 -6.80 -11.03 -10.59 -5.17
Vacuole Periphery 5.20 2.38 2.73 -2.81 0.37 2.86 5.46 -0.64 -3.62 -6.53 5.40 5.58 1.66 -4.14 -4.30
Standard name
Human Ortholog
Description Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex
Localization
Cell Percentages ER (97%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Ost4

Ost4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ost4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available