Standard name
Human Ortholog
Description Protein of unknown function; overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0 0.06 0 0 0 0 – 0 0 0.11 0.1 0.12 0.15 0.15 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.15 0.18 0.12 0.37 – 0.4 0.25 0.05 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.96 0.9 0.92 0.93 0.9 0.88 0.89 0.87 0.85 – 0.83 0.83 0.86 0.89 0.85 0.78 0.75 0.79 0.88 0.85 0.82 0.64 0.56 0.51
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.1 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.09 0.09 0.06 0.06 0.1 0.22 0.26 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 1 0 2 4
Bud 0 0 1 0 2 2 4 1 9 – 9 8 1 0 0 0 0 1 0 6 4 4 17 7
Bud Neck 0 0 7 1 3 0 3 2 1 – 0 1 0 0 0 0 0 1 0 0 0 1 4 3
Bud Site 0 1 0 1 1 5 3 4 16 – 15 17 2 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 14 35 17 35 20 32 32 17 – 13 25 30 41 56 45 62 34 2 7 7 4 12 11
Endoplasmic Reticulum 0 1 1 1 0 0 0 0 0 – 0 0 0 0 1 16 16 15 0 0 2 0 2 0
Endosome 0 3 0 0 1 2 0 3 0 – 0 1 0 1 3 4 15 4 4 2 2 0 3 5
Golgi 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 1 1 0 4
Mitochondria 12 1 11 15 14 78 142 87 214 – 213 145 14 3 7 12 7 17 3 4 6 3 6 12
Nucleus 247 321 526 357 503 457 718 622 494 – 446 490 233 366 407 236 315 248 234 322 501 118 249 209
Nuclear Periphery 0 0 0 0 0 0 3 2 5 – 3 3 3 2 2 1 2 0 0 1 0 0 1 1
Nucleolus 0 1 0 0 0 1 3 5 7 – 20 16 2 0 10 0 1 0 1 3 2 2 12 11
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 3 0 0 0 0 0 0 0
SpindlePole 0 1 5 5 10 4 9 15 3 – 4 1 1 1 0 1 2 2 1 4 8 2 11 15
Vac/Vac Membrane 3 34 2 4 15 11 14 12 9 – 10 17 5 4 13 21 37 27 15 23 62 40 117 110
Unique Cell Count 257 357 572 382 556 519 807 716 580 537 589 271 413 481 302 422 314 267 381 609 186 448 409
Labelled Cell Count 267 377 590 401 584 580 931 785 775 734 724 291 418 500 337 460 349 267 381 609 186 448 409


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.9 5.7 5.4 5.9 4.9 5.1 5.5 4.7 – 4.7 5.2 6.1 6.1 5.8 7.7 8.0 7.1 5.0 5.2 5.3
Std Deviation (1e-4) 0.8 0.9 1.1 1.2 1.0 0.8 0.8 1.0 0.9 – 1.0 1.1 1.9 2.0 1.4 6.0 1.9 1.4 1.7 1.9 1.6
Intensity Change (Log2) – – – -0.09 0.04 -0.23 -0.16 -0.07 -0.29 – -0.28 -0.12 0.09 0.09 0.02 0.44 0.48 0.31 -0.19 -0.15 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 4.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -1.1 0.1 -1.7 -1.8 -1.3 -2.6 – -3.0 -1.4 2.5 2.2 3.2 4.3 4.5 2.5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 5.2 4.3 4.9
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 4.5 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 1.9 0.7 0 9.1 6.9 0 – 0 11.3 2.6 -1.6 -0.6 1.8 -0.3 2.8
Nucleus 0.9 -0.9 -2.2 -1.8 -2.9 -3.6 – -4.5 -4.5 -2.7 -1.8 -3.7 -5.8 -7.5 -5.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 4.0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 1.4 0 0.4 1.8 0 – 0 0 0 0 0 0 0 0
Vacuole 0 3.2 2.7 2.4 2.3 2.1 – 2.4 3.4 0 0 3.2 5.8 6.8 6.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6603 3.6404 3.1176 2.7647 2.308 3.0032 1.8475 2.1654 1.8565 1.5886 2.0068 1.901 1.87 2.1775 1.8645 1.8809 1.9833 1.7122
Actin 0.0077 0 0 0.0387 0.0001 0.0007 0.0127 0 0.0025 0 0.0093 0.0021 0.0118 0 0.0027 0.009 0.0002 0
Bud 0.0002 0 0 0.0001 0 0 0.0008 0 0.0003 0 0.0004 0.002 0.0004 0 0.0002 0.0001 0.0001 0
Bud Neck 0.0011 0.0001 0.0001 0.0002 0.0002 0.0009 0.0021 0.0002 0.0016 0 0.0008 0.0005 0.0007 0.0002 0.0007 0.0003 0.0006 0.0005
Bud Periphery 0.0006 0 0 0.0002 0 0 0.0014 0 0.0007 0 0.0009 0.0004 0.0008 0 0.0003 0.0001 0.0001 0
Bud Site 0.0007 0.0001 0 0.0013 0 0 0.0081 0.0002 0.0026 0 0.0007 0.0004 0.0013 0.0001 0.0034 0.0001 0.0005 0
Cell Periphery 0.0001 0 0 0 0 0 0.0004 0 0.0001 0 0.0002 0 0.0002 0 0.0003 0 0.0001 0
Cytoplasm 0.0026 0.0005 0.0022 0.0003 0.0005 0.0005 0.0046 0.0007 0.0077 0.0002 0.0012 0.001 0.0035 0.0003 0.0011 0.002 0.003 0.0001
Cytoplasmic Foci 0.0013 0 0 0.0016 0.0002 0.0004 0.0081 0.0006 0.0034 0 0.0042 0.0012 0.0093 0 0.0023 0.0112 0.0048 0
Eisosomes 0.0001 0 0 0.0002 0 0 0.0001 0 0 0 0.0002 0 0.0002 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0009 0.0001 0.0002 0.0001 0 0.0001 0.0008 0.0001 0.0012 0 0.0016 0.0004 0.0024 0 0.0004 0.0013 0.0011 0
Endosome 0.0025 0 0 0.0007 0.0001 0.0004 0.0117 0.0007 0.0128 0 0.0095 0.0039 0.0085 0 0.0015 0.0048 0.0071 0
Golgi 0.0008 0 0 0.0013 0 0.0001 0.0049 0.0004 0.0043 0 0.0073 0.0011 0.0031 0 0.0008 0.0023 0.0004 0
Lipid Particles 0.0007 0 0 0.0014 0.0001 0.0004 0.0078 0.0007 0.005 0 0.015 0.0025 0.015 0 0.001 0.0248 0.0057 0
Mitochondria 0.001 0.0001 0.0001 0.0015 0.0001 0.0001 0.0092 0.001 0.008 0.0001 0.0149 0.0036 0.0063 0 0.0011 0.0019 0.002 0.0001
None 0.0126 0 0.0002 0.0004 0.0031 0.0014 0.0079 0.0001 0.0014 0 0.001 0.0004 0.004 0.0001 0.002 0.0016 0.0019 0
Nuclear Periphery 0.0047 0.0059 0.0021 0.0009 0.0012 0.0008 0.0233 0.0045 0.0077 0.0008 0.0196 0.0035 0.007 0.0017 0.0017 0.0076 0.0189 0.0005
Nucleolus 0.014 0.0044 0.0036 0.0025 0.0119 0.0136 0.0191 0.0062 0.0055 0.0035 0.0351 0.0123 0.0165 0.007 0.007 0.0067 0.0498 0.0165
Nucleus 0.9204 0.971 0.9833 0.9432 0.9487 0.9732 0.8541 0.9649 0.9163 0.9952 0.8425 0.9389 0.828 0.8452 0.859 0.8714 0.7136 0.8926
Peroxisomes 0.0003 0 0 0.005 0 0.0002 0.0049 0.0012 0.0017 0 0.0025 0.001 0.0082 0 0.003 0.0084 0.0002 0
Punctate Nuclear 0.0247 0.0175 0.0081 0.0004 0.0337 0.0072 0.0109 0.0182 0.0126 0.0002 0.0303 0.0241 0.0702 0.1452 0.1099 0.0457 0.1781 0.0896
Vacuole 0.0023 0.0002 0.0001 0 0 0.0001 0.0048 0.0004 0.003 0 0.0013 0.0003 0.0016 0 0.0015 0.0002 0.0063 0
Vacuole Periphery 0.0004 0.0001 0 0 0 0 0.0024 0.0001 0.0015 0 0.0015 0.0004 0.0009 0 0.0002 0.0003 0.0053 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.4851 24.058 19.7454 30.0764 20.6186 18.3391 24.4171 27.736 21.4248 29.8732
Translational Efficiency 0.9357 0.8402 1.0786 0.5866 0.93 1.4067 0.7268 0.6787 0.8754 0.7585

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1784 1650 129 1447 1437 1931 1685 42 3221 3581 1814 1489

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 692.88 758.68 1063.09 1017.12 705.11 780.54 1026.63 1123.97 698.34 770.47 1029.22 1020.13
Standard Deviation 82.35 120.59 146.65 150.54 91.41 102.80 143.57 204.41 86.73 111.88 144.10 153.34
Intensity Change Log 2 — 0.130886 0.617586 0.553812 — 0.146624 0.541996 0.672683 — 0.138845 0.579956 0.614991

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000060 0.000176 0.001575 0.001879 0.000091 0.000682 0.000507 0.001682 0.000074 0.000449 0.000583 0.001873
Bud Neck 0.002451 0.004585 0.005902 0.029848 0.001105 0.005746 0.012943 0.010600 0.001850 0.005211 0.012442 0.029305
Bud Site 0.001130 0.001344 0.001780 0.006753 0.000302 0.002676 0.001221 0.006591 0.000761 0.002062 0.001260 0.006748
Cell Periphery 0.000127 0.000120 0.000144 0.000118 0.000092 0.000092 0.000065 0.000495 0.000112 0.000105 0.000070 0.000128
Cytoplasm 0.026509 0.043043 0.009344 0.006516 0.048582 0.031785 0.001625 0.023613 0.036356 0.036973 0.002174 0.006998
Cytoplasmic Foci 0.002928 0.002257 0.001859 0.001120 0.003884 0.003831 0.000027 0.015924 0.003354 0.003106 0.000158 0.001538
Eisosomes 0.000014 0.000013 0.000078 0.000027 0.000015 0.000019 0.000024 0.000041 0.000015 0.000016 0.000028 0.000027
Endoplasmic Reticulum 0.001042 0.002732 0.012186 0.003981 0.001539 0.003253 0.004166 0.007253 0.001264 0.003013 0.004736 0.004073
Endosome 0.000986 0.000581 0.000871 0.002193 0.000559 0.001154 0.000137 0.004582 0.000796 0.000890 0.000190 0.002261
Golgi 0.000384 0.000118 0.000142 0.001758 0.000094 0.000652 0.000040 0.040561 0.000255 0.000406 0.000047 0.002852
Lipid Particles 0.000753 0.000442 0.001351 0.000206 0.000933 0.001303 0.000055 0.001689 0.000833 0.000906 0.000147 0.000248
Mitochondria 0.001682 0.001430 0.001060 0.003498 0.001549 0.004519 0.001084 0.002003 0.001622 0.003096 0.001082 0.003455
Mitotic Spindle 0.001432 0.001198 0.001527 0.013747 0.000313 0.006739 0.000324 0.008635 0.000933 0.004186 0.000409 0.013602
None 0.010921 0.012893 0.001615 0.004266 0.008796 0.004283 0.001537 0.033467 0.009973 0.008250 0.001543 0.005089
Nuclear Periphery 0.000468 0.000754 0.000899 0.001633 0.000857 0.001627 0.000400 0.000909 0.000642 0.001225 0.000436 0.001613
Nuclear Periphery Foci 0.000198 0.000313 0.000979 0.000224 0.000319 0.000549 0.000040 0.000745 0.000252 0.000440 0.000107 0.000238
Nucleolus 0.002659 0.004107 0.004398 0.002739 0.003552 0.004159 0.001854 0.005766 0.003057 0.004135 0.002035 0.002824
Nucleus 0.943954* 0.921285* 0.940440* 0.914090* 0.925634* 0.921393* 0.972026* 0.823369* 0.935781* 0.921343* 0.969780* 0.911531*
Peroxisomes 0.000655 0.000376 0.000503 0.000389 0.000250 0.001223 0.000066 0.000291 0.000474 0.000833 0.000097 0.000386
Vacuole 0.001510 0.001889 0.013169 0.003906 0.001275 0.003811 0.001740 0.011199 0.001405 0.002926 0.002553 0.004112
Vacuole Periphery 0.000136 0.000342 0.000178 0.001112 0.000259 0.000503 0.000118 0.000584 0.000191 0.000429 0.000122 0.001097

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.26 -9.96 -4.24 -3.87 2.07 -1.25 -20.93 -6.04 -2.45 -4.28 -1.49 -22.57 -4.38 -2.52 -2.16
Bud Neck -2.61 -5.39 -15.35 -14.03 -9.86 -6.52 -15.73 -5.31 -3.82 -0.67 -5.89 -14.65 -16.53 -14.22 -7.39
Bud Site -0.60 -3.00 -4.90 -4.90 -2.01 -3.79 -6.75 -1.72 -1.34 -1.52 -3.08 -3.22 -5.90 -4.22 -4.89
Cell Periphery 0.25 -2.24 0.89 0.76 3.42 0.04 2.78 -1.84 -1.84 -2.05 0.45 3.08 -0.37 -1.00 -4.50
Cytoplasm -4.86 4.39 9.21 12.06 0.94 4.19 14.11 1.88 0.61 -1.78 -0.33 18.12 14.14 14.95 -5.43
Cytoplasmic Foci 0.46 1.61 4.41 3.94 1.58 -0.20 5.97 -0.43 -0.40 -1.12 -0.04 7.91 5.66 6.10 -2.31
Eisosomes 0.10 -7.79 -8.33 -11.50 6.32 -2.20 -9.15 -4.49 -4.00 -3.06 -1.63 -11.68 -10.92 -9.83 1.16
Endoplasmic Reticulum -8.16 -11.04 -16.50 -8.00 7.98 -6.15 -14.35 -5.61 -3.96 -2.63 -9.76 -18.87 -17.69 -7.23 2.93
Endosome 0.75 -0.70 -0.40 -1.73 0.38 -2.46 2.60 -1.20 -0.78 -1.49 -0.42 2.04 -1.10 -0.97 -3.31
Golgi 1.86 0.95 -0.96 -1.54 -1.28 -1.94 1.50 -1.01 -0.97 -1.01 -0.91 2.47 -1.60 -1.31 -1.90
Lipid Particles 1.24 -1.81 2.38 3.11 3.62 -1.37 4.63 -0.32 0.40 -1.96 -0.59 4.50 3.80 4.85 -1.65
Mitochondria 0.35 0.60 -2.06 -2.86 -4.36 -2.66 0.28 -1.28 1.60 -2.19 -2.20 0.60 -2.49 -0.00 -4.36
Mitotic Spindle 0.03 -1.03 -5.82 -5.85 -4.18 -4.09 -0.56 -1.86 -0.56 -1.82 -3.36 0.37 -6.21 -4.72 -6.34
None -0.93 5.84 4.63 5.68 -3.04 4.45 7.80 -0.79 -1.23 -1.50 1.46 9.13 5.56 4.51 -5.16
Nuclear Periphery -5.67 -6.03 -6.56 -5.14 -2.74 -2.39 1.26 -1.30 0.35 -2.01 -3.56 -0.29 -5.46 -2.61 -5.69
Nuclear Periphery Foci -0.95 -1.57 -0.15 1.30 1.56 -1.63 3.25 -1.13 0.04 -2.50 -1.90 0.98 0.27 2.63 -1.04
Nucleolus -2.24 -1.25 -0.12 2.24 1.23 -0.97 2.76 -1.56 -1.11 -2.56 -2.30 1.99 0.58 2.81 -1.37
Nucleus 4.33 2.56 5.64 1.46 0.39 0.89 -8.45 2.50 2.38 3.42 3.84 -7.78 5.02 2.11 10.50
Peroxisomes 1.01 0.16 1.71 1.62 3.23 -4.35 4.84 -1.06 3.82 -3.54 -2.23 2.88 1.56 4.33 -2.36
Vacuole -1.28 -4.85 -5.94 -4.78 3.56 -4.13 -3.37 -2.02 -1.91 -1.96 -3.98 -5.24 -5.96 -4.15 -3.24
Vacuole Periphery -1.85 -2.81 -2.76 -1.27 -2.13 -1.99 1.57 -0.66 0.73 -1.64 -2.96 1.32 -2.40 -0.86 -2.86
Standard name
Human Ortholog
Description Protein of unknown function; overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Sok1

Sok1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sok1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available