Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0.05 0.05 0.07 – 0.05 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0.09 0.12 0.11 0 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0.08 0.06 0.06 0.2 0.11 0.11 0.26 – 0.21 0.27 0 0 0 0.17 0 0.15 0 0 0 0 0 0
Nucleus 0.27 0.27 0.14 0 0.05 0.05 0 0.05 0.05 – 0.06 0.06 0.07 0.05 0 0.11 0.1 0.12 0.13 0.23 0.06 0.07 0 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.71 0.75 0.86 0.92 0.89 0.84 0.82 0.83 0.81 – 0.8 0.82 0.87 0.79 0.81 0.79 0.83 0.81 0.73 0.64 0.75 0.79 0.72 0.68
Peroxisomes 0 0 0 0 0 0 0.06 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.27 0.32 0.33 0.44 0.4 0.35 – 0.35 0.44 0.12 0.13 0.16 0 0 0 0 0 0.07 0.05 0.09 0.11
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.07 0 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 7 6 6 – 8 2 1 0 1 0 0 0 2 2 2 1 5 6
Bud Neck 0 1 3 9 4 4 8 7 5 – 6 3 10 18 11 1 0 0 2 2 1 2 8 8
Bud Site 0 0 2 1 3 11 17 20 20 – 12 21 1 1 6 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 3 5 5 4 8 10 6 9 – 8 2 26 50 51 16 7 17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 4 2 2 0 0 0 0 1 0
Endosome 0 4 3 0 16 5 10 7 3 – 6 3 4 12 8 6 2 4 0 1 9 4 5 11
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 2 0 0 0 0 1 1
Mitochondria 3 20 31 16 14 49 34 42 78 – 53 69 11 6 15 68 8 40 3 4 13 2 4 10
Nucleus 65 66 52 9 11 13 9 19 16 – 16 16 20 22 19 41 19 31 34 60 25 15 13 19
Nuclear Periphery 6 2 7 1 0 0 1 1 4 – 0 0 0 2 1 1 0 1 11 1 4 0 1 3
Nucleolus 170 185 325 242 215 204 261 315 241 – 202 213 256 343 369 307 165 214 199 167 301 171 243 293
Peroxisomes 2 1 0 5 6 7 19 13 7 – 3 10 14 14 9 5 3 1 0 0 1 1 4 7
SpindlePole 1 3 23 70 77 80 142 154 104 – 88 114 35 57 74 14 5 5 3 10 26 10 28 46
Vac/Vac Membrane 9 5 2 0 0 0 1 0 2 – 0 0 2 4 2 22 13 8 12 10 12 8 11 11
Unique Cell Count 241 247 379 262 242 244 320 381 299 251 260 293 434 457 390 199 264 272 264 402 219 336 434
Labelled Cell Count 258 290 453 358 350 381 519 590 495 402 453 380 530 567 486 224 325 272 264 402 219 336 434


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.7 18.0 13.2 11.7 10.8 8.3 8.1 7.7 7.0 – 6.6 6.5 16.9 15.3 13.3 24.3 26.5 25.2 20.0 19.4 19.5
Std Deviation (1e-4) 6.7 6.4 4.3 3.2 2.6 2.1 1.9 2.0 1.9 – 1.9 1.9 6.8 7.0 6.1 10.0 9.6 9.2 7.7 6.4 6.1
Intensity Change (Log2) – – – -0.18 -0.3 -0.66 -0.7 -0.78 -0.91 – -1.01 -1.03 0.36 0.21 0.01 0.88 1.0 0.94 0.6 0.55 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 1.8 0 0 – 0 0 2.4 3.0 1.8 0 0 0
Bud Site 0 0 3.4 3.9 3.9 4.5 – 3.5 5.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.7 1.6 0.3 1.5 – 1.6 0 4.6 5.8 5.7 2.4 0 3.5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 4.1 0 2.3 0 0 – 0 0 0 2.1 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria -1.0 -1.1 4.3 1.1 1.3 6.3 – 4.7 6.3 -2.4 -4.6 -3.1 3.8 -1.9 2.8
Nucleus -4.4 -3.7 -3.3 -5.1 -4.1 -3.6 – -2.9 -3.0 -2.9 -4.3 -4.9 -1.4 -1.5 -0.7
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 2.6 1.1 -0.7 -1.5 -1.2 -1.8 – -1.8 -1.3 0.6 -2.5 -1.9 -2.5 -0.9 -1.6
Peroxisomes 0 0 0 4.8 3.6 0 – 0 0 4.3 3.5 0 0 0 0
SpindlePole 7.3 8.5 8.8 11.9 11.2 9.5 – 9.4 11.4 2.7 3.4 4.6 -1.6 -1.9 -2.6
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 4.1 4.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.1626 21.3294 19.6032 17.9241 18.0698 18.8047 14.3075 17.4067 16.2271 15.1679 15.8423 15.4054 19.6661 22.9486 21.7497 19.9478 19.5091 20.3704
Actin 0.0091 0 0 0 0 0 0.0025 0 0.0023 0.0001 0 0.0001 0.0303 0 0 0 0.0001 0
Bud 0.0004 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0 0 0.0001 0
Bud Neck 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001
Bud Periphery 0.0004 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0.0001 0 0.0001 0.0001 0 0 0 0.0001 0
Bud Site 0.0027 0.0002 0.0006 0 0.0002 0 0.0002 0.0001 0.0003 0.0003 0 0.0001 0.0004 0.0001 0 0.0001 0.0002 0.0001
Cell Periphery 0.0003 0.0002 0.0016 0.0002 0.0001 0.0003 0.0002 0.0003 0.0001 0.0003 0.0001 0.0002 0.0004 0.0005 0.0001 0.0002 0.0001 0.0002
Cytoplasm 0.0027 0.0001 0 0 0 0 0.0011 0 0.0001 0.0001 0 0.0002 0.001 0 0 0 0 0
Cytoplasmic Foci 0.0208 0.0042 0.0028 0.0001 0.0005 0.0005 0.0032 0.0002 0.0039 0.0017 0.0011 0.0016 0.0217 0.0003 0.0005 0.0002 0.0126 0.0007
Eisosomes 0.0004 0 0 0 0 0 0.0001 0 0.0001 0.0001 0 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0004 0 0 0 0 0 0.001 0 0.0001 0.0001 0 0.0001 0.0004 0 0 0 0 0
Endosome 0.0124 0.0055 0.0003 0 0 0 0.0052 0 0.0007 0.0001 0 0.0002 0.0036 0.0002 0 0 0.0001 0
Golgi 0.0024 0.0003 0.0001 0 0.0001 0 0.0014 0 0.0003 0.0001 0 0.0002 0.0016 0.0002 0 0 0.0001 0.0001
Lipid Particles 0.0219 0.0029 0.0039 0.0001 0.0017 0.0051 0.0103 0.0011 0.0021 0.0024 0.004 0.013 0.0077 0.0089 0.0009 0.0005 0.0018 0.0102
Mitochondria 0.0184 0.0008 0.0025 0.0002 0.0027 0.0313 0.0051 0.0001 0.0016 0.0024 0.0001 0.0121 0.0018 0.0009 0.0003 0.0002 0.009 0.0091
None 0.002 0 0.0002 0 0.0001 0 0.0008 0 0.0006 0.0006 0 0.0002 0.0059 0 0 0 0.0001 0
Nuclear Periphery 0.0047 0.0001 0.0001 0 0.0003 0.0001 0.0041 0.0018 0.0001 0.0012 0.0003 0.0013 0.0022 0.0001 0.0007 0 0.0012 0.0003
Nucleolus 0.7841 0.8524 0.9155 0.9257 0.9224 0.903 0.9087 0.906 0.9225 0.8899 0.9135 0.9104 0.8462 0.9045 0.9324 0.9555 0.8845 0.9098
Nucleus 0.0805 0.1308 0.0656 0.0732 0.0693 0.0559 0.05 0.0866 0.0627 0.0919 0.0728 0.0565 0.0505 0.0776 0.0608 0.0427 0.0842 0.061
Peroxisomes 0.0045 0.0003 0.0003 0 0.0002 0.0001 0.0023 0 0.0007 0.0002 0 0.0002 0.0029 0.0001 0 0 0.0009 0.0002
Punctate Nuclear 0.0268 0.0001 0.0005 0.0001 0.0015 0.0013 0.0023 0.0007 0.0009 0.0062 0.0078 0.0015 0.0211 0.0009 0.0039 0.0002 0.0042 0.0012
Vacuole 0.0031 0.0014 0.0041 0.0002 0.0002 0.0004 0.0005 0.0009 0.0003 0.001 0.0001 0.0004 0.0006 0.0017 0.0002 0.0002 0.0002 0.0005
Vacuole Periphery 0.0016 0.0005 0.0014 0.0001 0.0004 0.0017 0.0008 0.0021 0.0002 0.001 0.0001 0.0018 0.001 0.0039 0.0001 0.0001 0.0004 0.0063

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 101.0916 96.7789 79.8992 64.928 84.3587 55.8921 62.9403 49.2051 42.8649 75.4531
Translational Efficiency 1.1838 1.2685 1.1292 1.1179 1.0562 1.3151 1.0003 1.0387 1.0474 1.0181

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
Localization
Cell Percentages nucleolus (80%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Mak21

Mak21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mak21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available