Standard name
Human Ortholog
Description Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.98 0.99 0.9 0.9 – 0.8 0.79 0.64 0.77 0.99 0.99 0.99 0.95 0.91 0.96 0.94 0.91 0.96 0.97 0.94 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.25 0.06 – 0.28 0.31 0.46 0.33 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 3 0 2 0 1 0 0 0 1
Bud 0 1 0 1 0 2 0 – 4 2 6 4 0 1 0 1 0 1 0 0 0 0 4 6
Bud Neck 0 0 0 0 0 1 1 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 4 7 5 2 12 – 8 4 2 7 1 3 6 4 4 2 0 0 0 0 0 0
Cytoplasm 119 237 159 213 268 178 338 – 257 292 134 203 253 499 444 201 140 133 116 227 155 207 392 213
Endoplasmic Reticulum 0 2 0 2 0 0 0 – 0 0 0 0 1 0 0 12 6 4 0 0 0 1 3 2
Endosome 0 0 0 1 0 0 5 – 4 5 2 2 1 3 4 4 6 1 0 0 0 0 1 5
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 1 2 0 1 1 1
Mitochondria 0 5 0 5 5 49 21 – 90 113 98 86 0 0 3 7 1 0 3 6 1 0 3 6
Nucleus 0 0 0 0 0 0 1 – 3 1 3 1 1 1 0 1 3 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 1 0 0 0 0 0 1 0
Nucleolus 0 1 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 2 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 2 0 7 – 2 2 4 2 0 0 1 2 6 1 0 0 0 0 2 5
Vac/Vac Membrane 1 2 1 2 4 2 23 – 19 9 4 5 0 0 0 2 1 3 0 7 0 0 0 5
Unique Cell Count 122 243 160 218 270 198 375 322 369 211 264 256 503 449 212 154 139 125 251 162 213 418 252
Labelled Cell Count 123 251 164 232 284 235 408 389 431 255 311 258 508 459 237 168 149 125 251 162 213 418 252


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.5 6.0 6.3 6.2 4.6 5.4 – 4.7 4.6 4.7 4.5 5.9 6.1 6.3 5.8 6.1 6.1 5.5 5.7 6.1
Std Deviation (1e-4) 0.8 1.1 1.0 2.8 1.4 2.8 1.5 – 1.4 1.4 1.7 0.8 1.2 1.1 1.8 1.0 1.4 1.2 1.5 1.6 2.0
Intensity Change (Log2) – – – 0.07 0.06 -0.37 -0.15 – -0.35 -0.38 -0.36 -0.42 -0.01 0.03 0.08 -0.05 0.04 0.02 -0.12 -0.08 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -0.1 -3.8 -3.8 – -5.9 -6.0 -8.4 -6.4 -0.6 -0.2 -0.5 -2.5 -3.5 -2.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.1 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.8 – 2.7 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4033 -0.1144 -0.1573 -0.47 -0.5375 -0.3058 2.1106 1.8186 2.0223 0.8028 1.4749 1.5219 3.2793 4.2243 2.8155 3.0361 3.1821 2.8816
Actin 0.0173 0.001 0.0252 0.0002 0.0332 0.0044 0.0307 0.0073 0.0114 0.0002 0.0021 0.0023 0.1203 0.0126 0.0002 0.0012 0.025 0.0015
Bud 0.0006 0.0016 0.0012 0.0001 0.0003 0.0001 0.0006 0.0041 0.0018 0.0001 0.0011 0.0002 0.0007 0.0004 0 0 0.0007 0
Bud Neck 0.0017 0.0002 0.0035 0.0001 0.0004 0.0004 0.0004 0.0002 0.0011 0.0001 0.0004 0.0002 0.0076 0.0026 0 0.0001 0.0001 0.0001
Bud Periphery 0.0013 0.0016 0.0023 0 0.0004 0 0.0007 0.0022 0.0022 0 0.0032 0.0005 0.0006 0.0003 0 0 0.0012 0
Bud Site 0.0013 0.0073 0.005 0 0.0005 0 0.0011 0.006 0.0027 0.0001 0.0003 0 0.0575 0.1476 0.0001 0 0.0004 0
Cell Periphery 0.0002 0.0003 0.0002 0 0.0001 0 0.0004 0.0002 0.0004 0.0001 0.0001 0.0001 0.0003 0.0007 0.0001 0 0.0001 0
Cytoplasm 0.2552 0.382 0.2907 0.5187 0.3725 0.3701 0.4557 0.6061 0.5475 0.817 0.6931 0.6112 0.0527 0.5287 0.4356 0.4178 0.3348 0.5593
Cytoplasmic Foci 0.0803 0.006 0.0232 0.0064 0.0123 0.0163 0.0053 0.0087 0.0088 0.0082 0.0215 0.004 0.1023 0.004 0.0019 0.0078 0.0224 0.0095
Eisosomes 0.0002 0.0001 0.0002 0 0.0001 0 0.0002 0.0001 0.0001 0.0001 0 0 0.001 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0061 0.0019 0.0075 0.0059 0.0014 0.0008 0.0229 0.0038 0.0108 0.0063 0.0057 0.0057 0.0018 0.0026 0.003 0.0016 0.001 0.0015
Endosome 0.0376 0.0018 0.0145 0.0051 0.0145 0.0027 0.0104 0.0016 0.0056 0.0062 0.0129 0.0019 0.0234 0.0007 0.0005 0.0007 0.0038 0.001
Golgi 0.0129 0.0001 0.0028 0.0002 0.0353 0.0002 0.0016 0.0004 0.001 0.0004 0.0008 0.0001 0.0093 0.0004 0.0001 0.0001 0.0126 0.0003
Lipid Particles 0.0166 0.0002 0.0089 0.0002 0.0078 0.0003 0.001 0.0004 0.0007 0.0047 0.0011 0.0001 0.0356 0.0002 0.0001 0.0001 0.0011 0.0029
Mitochondria 0.0075 0.0001 0.0014 0.0002 0.0078 0.0002 0.0022 0.0003 0.0009 0.0003 0.0041 0.0006 0.0028 0.0001 0.0001 0.0001 0.0041 0.0002
None 0.5167 0.5922 0.5888 0.4597 0.4867 0.6018 0.4181 0.3536 0.3812 0.1511 0.1919 0.3581 0.5225 0.2968 0.5566 0.5686 0.5633 0.4136
Nuclear Periphery 0.0157 0.0007 0.0062 0.0009 0.0004 0.0003 0.0168 0.001 0.0078 0.0012 0.017 0.0103 0.0093 0.0004 0.0005 0.0003 0.0002 0.0002
Nucleolus 0.0007 0.0001 0.0003 0 0.0001 0 0.0003 0.0001 0.0003 0.0001 0.0008 0 0.0006 0 0 0 0 0
Nucleus 0.0047 0.0011 0.0018 0.0008 0.0006 0.0006 0.0227 0.0009 0.0093 0.0009 0.031 0.0028 0.0011 0.0005 0.0005 0.0005 0.0002 0.0003
Peroxisomes 0.0095 0.0001 0.0087 0 0.0239 0.0006 0.001 0.0008 0.0003 0.0001 0.0005 0.0001 0.017 0.0002 0 0.0005 0.0282 0.0082
Punctate Nuclear 0.0092 0.0005 0.0047 0.0004 0.0007 0.0006 0.0026 0.0012 0.0038 0.0004 0.0008 0.0008 0.0325 0.0003 0.0002 0.0002 0.0002 0.0011
Vacuole 0.0027 0.001 0.0019 0.0009 0.0007 0.0004 0.0036 0.0007 0.0018 0.0021 0.006 0.0005 0.0005 0.0005 0.0003 0.0003 0.0004 0.0002
Vacuole Periphery 0.0018 0.0001 0.0012 0.0002 0.0003 0.0001 0.0014 0.0001 0.0005 0.0003 0.0056 0.0003 0.0005 0 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 0.9335 3.338 1.2385 4.5288 2.4207 1.866 5.4844 3.9864 4.459 5.6578
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
103 750 106 1952 1494 2408 340 1593 1597 3158 446 3545

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 655.66 786.08 1084.94 866.73 712.03 809.34 1006.23 905.13 708.39 803.82 1024.94 883.99
Standard Deviation 79.35 107.75 130.58 111.79 83.49 98.19 123.30 122.94 84.37 101.03 129.48 118.48
Intensity Change Log 2 — 0.261728 0.726595 0.402635 — 0.184808 0.498950 0.346187 — 0.222195 0.612569 0.373523

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000224 0.000325 0.001056 0.000474 0.000120 0.000387 0.000509 0.001657 0.000127 0.000372 0.000639 0.001006
Bud Neck 0.031962 0.011265 0.003873 0.004871 0.014069 0.017057 0.002834 0.008147 0.015223 0.015681 0.003081 0.006343
Bud Site 0.009983 0.014696 0.004952 0.025351 0.004745 0.013765 0.004047 0.039872 0.005083 0.013986 0.004262 0.031876
Cell Periphery 0.000429 0.000119 0.000321 0.000098 0.000108 0.000114 0.000189 0.000315 0.000129 0.000115 0.000221 0.000196
Cytoplasm 0.390692* 0.573117* 0.278973* 0.593223* 0.551030* 0.611965* 0.482597* 0.593591* 0.540689* 0.602739* 0.434202* 0.593389*
Cytoplasmic Foci 0.343927* 0.194980* 0.017588 0.030045 0.200063* 0.104705 0.012782 0.037367 0.209341* 0.126145 0.013924 0.033335
Eisosomes 0.000378 0.000060 0.000104 0.000037 0.000109 0.000052 0.000053 0.000035 0.000127 0.000054 0.000065 0.000036
Endoplasmic Reticulum 0.000678 0.001464 0.012150 0.002264 0.000709 0.000569 0.010100 0.001000 0.000707 0.000781 0.010587 0.001696
Endosome 0.009303 0.016761 0.009608 0.006865 0.009160 0.007065 0.004675 0.008053 0.009169 0.009368 0.005847 0.007399
Golgi 0.005409 0.001583 0.000208 0.002237 0.001746 0.001295 0.000174 0.002761 0.001982 0.001363 0.000182 0.002473
Lipid Particles 0.010092 0.003155 0.004385 0.000988 0.004203 0.001892 0.001915 0.000903 0.004583 0.002192 0.002502 0.000950
Mitochondria 0.010793 0.002211 0.000427 0.004969 0.003512 0.004759 0.000428 0.005856 0.003982 0.004154 0.000428 0.005368
Mitotic Spindle 0.000132 0.002289 0.002513 0.046425 0.003643 0.006799 0.002426 0.063608 0.003417 0.005728 0.002447 0.054146
None 0.001292 0.002615 0.001629 0.005837 0.001739 0.003497 0.007882 0.005447 0.001710 0.003287 0.006396 0.005662
Nuclear Periphery 0.000352 0.000684 0.002923 0.001034 0.000434 0.000538 0.001816 0.000742 0.000429 0.000573 0.002079 0.000903
Nuclear Periphery Foci 0.000228 0.001019 0.011094 0.002453 0.000306 0.000190 0.006203 0.001743 0.000301 0.000387 0.007366 0.002134
Nucleolus 0.001435 0.000802 0.000875 0.000361 0.000651 0.000882 0.000307 0.000628 0.000702 0.000863 0.000442 0.000481
Nucleus 0.138548* 0.085081 0.388908* 0.105142 0.152184* 0.161879* 0.301794* 0.098976 0.151305* 0.143640* 0.322498* 0.102371
Peroxisomes 0.016750 0.005462 0.000401 0.001293 0.003018 0.002640 0.000227 0.004306 0.003904 0.003310 0.000268 0.002647
Vacuole 0.026500 0.080968 0.257675* 0.163239 0.047793 0.058431 0.158497 0.122441 0.046420 0.063783 0.182068 0.144906
Vacuole Periphery 0.000896 0.001343 0.000338 0.002794 0.000658 0.001521 0.000545 0.002552 0.000673 0.001479 0.000496 0.002685

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.40 -5.45 -1.94 0.00 4.87 -1.24 -8.82 -2.62 -0.95 -0.03 -1.50 -10.35 -3.66 -0.59 2.63
Bud Neck 2.81 3.70 3.71 4.69 -0.20 -2.09 8.66 4.16 6.71 -5.66 -0.36 9.22 7.44 9.89 -4.04
Bud Site -1.32 1.34 -1.27 0.11 -5.16 -6.78 -1.47 -8.80 -3.31 -8.41 -7.83 -1.06 -9.86 -2.16 -9.75
Cell Periphery 3.28 1.12 3.75 5.69 6.65 -0.43 -6.48 -0.40 -0.24 1.12 0.85 -6.08 1.27 0.67 4.58
Cytoplasm -6.88 5.36 -4.66 5.22 -14.29 -6.63 12.61 7.42 14.48 -7.71 -7.09 15.94 5.94 15.70 -13.10
Cytoplasmic Foci 6.14 14.14 13.65 23.68 -4.77 16.45 36.66 31.59 21.08 -10.19 14.54 38.60 35.33 31.76 -12.06
Eisosomes 5.81 4.80 6.20 6.19 6.87 7.50 5.07 10.00 2.40 6.82 9.19 5.60 11.67 4.00 9.64
Endoplasmic Reticulum -3.57 -7.58 -2.29 -0.20 6.76 1.44 -6.00 -5.93 -6.84 5.52 -0.95 -8.29 -3.39 -2.94 7.37
Endosome -3.59 0.06 3.54 7.58 4.53 2.84 5.20 4.45 2.34 -1.27 -0.16 4.48 6.37 7.56 1.07
Golgi 1.65 2.24 1.96 2.09 -2.59 1.36 6.62 2.33 1.02 -2.91 1.92 6.73 3.47 1.83 -3.91
Lipid Particles 2.38 1.92 3.05 5.25 5.79 6.71 5.22 8.36 2.77 5.40 6.37 4.40 9.07 5.99 8.23
Mitochondria 1.27 1.52 1.29 0.17 -2.50 -1.03 3.30 1.49 3.02 -2.34 -0.12 3.63 1.94 3.02 -3.42
Mitotic Spindle -2.71 -1.63 -10.04 -8.86 -8.01 -2.06 -2.05 -13.18 -12.54 -9.99 -1.80 -1.98 -15.00 -14.51 -10.43
None -2.41 0.06 -4.81 -2.94 -5.31 -3.52 -5.48 -10.66 -4.56 -2.04 -3.82 -5.00 -8.57 -3.97 -2.43
Nuclear Periphery -1.22 -8.89 -10.00 -3.88 4.52 -0.92 -13.71 -15.42 -10.94 2.74 -1.31 -16.04 -17.41 -10.88 5.48
Nuclear Periphery Foci -7.28 -7.35 -5.10 -2.99 5.68 3.37 -5.88 -8.91 -9.30 3.74 -2.21 -7.87 -8.88 -8.51 5.56
Nucleolus 0.86 1.02 0.93 0.58 -0.41 -1.74 -0.11 -4.87 -3.10 -2.63 -1.43 0.08 -3.66 -1.80 -1.76
Nucleus 2.78 -6.07 0.10 -8.38 7.20 -1.65 -11.58 3.51 5.66 13.37 1.19 -13.10 3.42 2.66 15.18
Peroxisomes 1.93 2.87 2.77 3.93 -3.14 0.84 8.01 1.38 0.66 -5.90 0.96 7.33 4.47 4.46 -6.74
Vacuole -5.08 -11.13 -20.45 -23.50 3.53 -4.12 -15.12 -27.80 -25.86 2.11 -7.00 -18.56 -42.55 -38.52 2.95
Vacuole Periphery -1.58 2.50 -0.23 0.74 -1.68 -2.45 3.51 -0.45 1.74 -1.97 -2.94 4.33 -0.88 1.56 -2.43
Standard name
Human Ortholog
Description Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (54%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rtr2

Rtr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available