Standard name
Human Ortholog
Description 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; abundance relative to Bmh1p increases during sporulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.98 0.94 0.99 0.94 0.93 0.96 0.91 0.92 0.93 0.99 0.99 0.98 1.0 0.99 0.98 0.98 0.93 0.96 0.95 0.92 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.07 0 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 2 1 3 6 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 1 0 1 1 4 6 9 8 9 9 6 1 2 2 2 5 2 0 0 0 0 0 0
Cytoplasm 525 330 182 126 78 205 235 285 222 184 169 192 392 555 523 246 172 176 504 315 177 377 339 356
Endoplasmic Reticulum 1 3 6 2 2 2 12 20 9 14 12 11 3 6 3 6 3 5 1 0 0 1 2 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 2 1 0 0 3 1 0 0 0
Nucleus 1 0 0 1 0 0 0 1 1 0 0 1 0 0 0 0 0 0 2 6 0 4 4 4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 2 4 0 1 2 0 2 0 0 0 3 0 0 2 7 0 1 2 0 2 1 4 2 3
Unique Cell Count 528 337 187 129 83 208 250 307 232 202 184 207 394 561 532 247 174 179 513 339 184 398 370 382
Labelled Cell Count 533 338 189 131 85 211 255 315 240 212 193 210 396 565 536 256 183 185 513 339 184 398 370 382


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 87.4 79.9 64.8 64.2 56.3 67.1 66.9 69.9 66.5 68.2 73.0 67.5 77.7 79.4 79.9 58.6 72.8 75.6 76.1 77.7 78.7
Std Deviation (1e-4) 15.0 20.3 16.3 18.4 20.9 19.2 18.5 20.2 18.6 20.2 21.1 21.4 18.7 18.0 20.7 19.9 27.9 27.2 19.5 20.9 21.0
Intensity Change (Log2) – – – -0.01 -0.2 0.05 0.05 0.11 0.04 0.07 0.17 0.06 0.26 0.29 0.3 -0.14 0.17 0.22 0.23 0.26 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 -1.3 0.9 -1.6 -2.1 -0.9 -2.6 -2.3 -2.1 2.2 1.6 0.8 2.0 1.0 0.7
Endoplasmic Reticulum 0 0 0 0.8 1.6 0.4 1.7 1.5 1.0 0 0 0 -0.5 0 -0.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 71.9576 81.6886 74.8344 79.3369 68.4059 77.0064 88.243 96.6514 90.9209 81.2275 78.877 97.0156 77.262 86.5782 82.3446 81.2245 77.5175 84.7577
Actin 0.0181 0.0002 0.0087 0 0.0029 0.0026 0.0054 0.0001 0.0046 0.0011 0.0065 0 0.0005 0.0001 0.0001 0.0001 0.0042 0.0001
Bud 0.0006 0 0.0002 0.0002 0.0008 0 0.0002 0 0.0002 0.0002 0.0003 0 0.0013 0 0 0 0.0005 0
Bud Neck 0.0005 0.0001 0.0005 0.0003 0.0005 0.0007 0.0002 0.0001 0.0002 0.0001 0.0003 0.0005 0.0002 0.0001 0.0001 0.0003 0.0005 0.0004
Bud Periphery 0.0006 0 0.0002 0.0006 0.0032 0 0.0001 0 0.0001 0.0001 0.0003 0 0.0012 0 0 0 0.0014 0
Bud Site 0.0022 0 0.0002 0 0.0021 0 0.0004 0 0.0005 0.0008 0.0016 0 0.0003 0 0 0 0.0002 0
Cell Periphery 0.0006 0.0001 0.0002 0.0006 0.0011 0 0.0005 0 0.0001 0.0004 0.0005 0 0.0003 0.0001 0 0 0.0002 0
Cytoplasm 0.8982 0.9954 0.96 0.9939 0.873 0.9703 0.9466 0.9985 0.9772 0.9562 0.8688 0.998 0.9401 0.9969 0.9919 0.9972 0.885 0.9951
Cytoplasmic Foci 0.0089 0.0004 0.0024 0.0001 0.0131 0.0004 0.0051 0.0001 0.0057 0.0109 0.0195 0.0001 0.0099 0.0001 0.0016 0.0002 0.0163 0.0003
Eisosomes 0.0003 0 0.0001 0 0.0005 0 0.0002 0 0.0001 0.0015 0.0003 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0046 0.0004 0.0006 0.0009 0.0058 0.0011 0.0011 0.0001 0.0007 0.0002 0.0023 0.0001 0.0015 0.0003 0.0002 0.0001 0.0012 0.0007
Endosome 0.0087 0.0001 0.0004 0 0.008 0.0056 0.0016 0 0.0004 0.0003 0.0176 0 0.0013 0 0.0001 0 0.0047 0.0001
Golgi 0.0034 0 0.0003 0 0.0018 0.0007 0.0008 0 0.0004 0.0003 0.0096 0 0.0002 0 0 0 0.0004 0
Lipid Particles 0.0046 0.0002 0.0022 0 0.0193 0.0005 0.0022 0 0.0018 0.0043 0.0132 0 0.003 0 0.0002 0 0.0016 0
Mitochondria 0.0061 0 0.0009 0.0001 0.0199 0.0004 0.0013 0 0.0006 0.0102 0.0059 0 0.0068 0 0 0 0.004 0
None 0.009 0.0006 0.0089 0.0001 0.0122 0.0008 0.0046 0.0001 0.0021 0.0066 0.012 0 0.0061 0.0001 0.0018 0.0001 0.0028 0.0004
Nuclear Periphery 0.0102 0.0004 0.0009 0.0005 0.006 0.0112 0.0073 0.0002 0.0006 0.0003 0.0081 0.0002 0.0051 0.0008 0.0004 0.0003 0.0365 0.0006
Nucleolus 0.0023 0.0001 0.001 0 0.0045 0.0002 0.0011 0 0.0003 0.002 0.006 0 0.0032 0 0.0004 0 0.0079 0
Nucleus 0.0109 0.001 0.0037 0.0013 0.0082 0.0031 0.0091 0.0008 0.0015 0.0013 0.0063 0.001 0.0075 0.0012 0.0014 0.0014 0.015 0.0017
Peroxisomes 0.0018 0 0.0061 0 0.0027 0.0001 0.0017 0 0.0012 0.0013 0.002 0 0.0007 0 0 0 0.0001 0
Punctate Nuclear 0.0038 0.0001 0.001 0 0.0073 0.0018 0.0071 0 0.0012 0.0004 0.0123 0 0.0024 0 0.0014 0 0.0044 0.0001
Vacuole 0.0036 0.0008 0.0014 0.0011 0.0049 0.0002 0.0025 0.0001 0.0006 0.0007 0.0039 0 0.0059 0.0002 0.0001 0.0002 0.0084 0.0004
Vacuole Periphery 0.0011 0.0001 0.0003 0.0001 0.0022 0.0005 0.0013 0 0.0002 0.0007 0.0025 0 0.0022 0 0 0 0.0048 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 377.1979 350.3915 348.1582 379.7615 331.547 372.9643 456.8035 495.8371 420.6139 355.9
Translational Efficiency 1.9603 2.0889 2.048 1.8293 1.9884 2.5314 1.9128 1.8186 2.0048 2.1258

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
67 1259 2504 1823 1528 2336 1156 1845 1595 3595 3660 3668

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 8265.20 4906.15 5366.73 6984.45 4487.51 5121.79 5469.61 7076.70 4646.20 5046.27 5399.22 7030.85
Standard Deviation 3324.96 1240.18 1087.31 1687.07 972.42 908.86 1617.79 1694.01 1394.48 1042.10 1279.75 1691.19
Intensity Change Log 2 — -0.752458 -0.623006 -0.242903 — 0.190733 0.285523 0.657162 — -0.346779 -0.234926 0.140911

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001403 0.000193 0.000187 0.000411 0.000091 0.000227 0.000483 0.000201 0.000146 0.000215 0.000280 0.000306
Bud Neck 0.002661 0.002472 0.001783 0.002997 0.000734 0.001857 0.005349 0.001567 0.000815 0.002072 0.002909 0.002278
Bud Site 0.019035 0.006014 0.007792 0.016371 0.002305 0.007615 0.009703 0.007433 0.003008 0.007054 0.008396 0.011875
Cell Periphery 0.212606* 0.003891 0.002809 0.009345 0.003205 0.001152 0.006677 0.005596 0.012001 0.002111 0.004031 0.007459
Cytoplasm 0.272973* 0.808776* 0.869128* 0.784101* 0.854710* 0.868925* 0.695801* 0.854496* 0.830273* 0.847860* 0.814383* 0.819509*
Cytoplasmic Foci 0.020332 0.013296 0.005544 0.004891 0.008569 0.008839 0.014008 0.003922 0.009064 0.010400 0.008218 0.004404
Eisosomes 0.000023 0.000004 0.000003 0.000009 0.000002 0.000002 0.000009 0.000004 0.000002 0.000003 0.000005 0.000007
Endoplasmic Reticulum 0.000931 0.000072 0.000030 0.000071 0.000038 0.000052 0.000120 0.000017 0.000076 0.000059 0.000058 0.000044
Endosome 0.004162 0.000295 0.000240 0.000705 0.000102 0.000384 0.000723 0.000050 0.000273 0.000353 0.000393 0.000375
Golgi 0.022823 0.002264 0.000693 0.000641 0.002627 0.001188 0.001287 0.000323 0.003476 0.001565 0.000881 0.000481
Lipid Particles 0.110798* 0.018323 0.009765 0.012651 0.004165 0.006569 0.014486 0.007726 0.008644 0.010686 0.011256 0.010174
Mitochondria 0.001655 0.000150 0.000077 0.000204 0.000112 0.000151 0.000231 0.000067 0.000177 0.000151 0.000126 0.000135
Mitotic Spindle 0.002267 0.001188 0.000196 0.001748 0.000407 0.000845 0.000687 0.000050 0.000486 0.000966 0.000351 0.000894
None 0.002142 0.013713 0.004069 0.005410 0.011638 0.011103 0.012306 0.005473 0.011239 0.012017 0.006670 0.005442
Nuclear Periphery 0.002703 0.000307 0.000121 0.000158 0.000202 0.000109 0.000385 0.000111 0.000307 0.000178 0.000204 0.000134
Nuclear Periphery Foci 0.017395 0.010659 0.003070 0.002205 0.004159 0.005676 0.004760 0.002439 0.004715 0.007421 0.003604 0.002323
Nucleolus 0.001291 0.000290 0.000116 0.000226 0.000072 0.000160 0.000274 0.000092 0.000123 0.000206 0.000166 0.000159
Nucleus 0.027306 0.082510 0.045354 0.026177 0.091576 0.063599 0.145820* 0.045768 0.088876 0.070221 0.077085 0.036031
Peroxisomes 0.000287 0.000050 0.000147 0.000127 0.000023 0.000127 0.000062 0.000073 0.000034 0.000100 0.000120 0.000100
Vacuole 0.252440* 0.035026 0.048534 0.129569 0.014147 0.020992 0.085151 0.064397 0.024157 0.025907 0.060099 0.096788
Vacuole Periphery 0.024766 0.000508 0.000342 0.001985 0.001116 0.000426 0.001679 0.000192 0.002110 0.000455 0.000764 0.001083

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.56 1.52 1.29 -4.82 -4.11 -2.32 -7.12 -7.11 -0.31 3.55 -1.84 -4.58 -5.19 -2.22 -0.92
Bud Neck -0.58 0.72 -0.55 0.12 -4.62 -5.86 -13.72 -7.81 0.48 11.18 -9.05 -15.74 -12.99 -0.76 6.12
Bud Site 1.35 1.25 0.54 -5.47 -5.61 -6.96 -8.76 -8.58 0.16 2.14 -6.40 -8.93 -10.74 -4.28 -3.33
Cell Periphery 9.07 9.13 8.98 -3.19 -5.52 7.33 -4.46 -5.06 -11.53 -0.09 6.83 5.53 3.99 -10.63 -5.34
Cytoplasm -13.88 -15.44 -12.97 3.94 12.02 0.63 30.22 5.08 4.92 -26.62 1.34 12.89 8.07 8.74 -6.19
Cytoplasmic Foci -0.14 1.27 1.46 8.00 2.12 -2.74 -7.95 4.10 7.64 11.11 -4.68 -2.99 3.94 10.73 9.80
Eisosomes 2.79 2.78 2.03 -6.14 -6.58 -3.91 -14.44 -9.99 -8.14 7.05 -2.25 -10.74 -10.50 -10.58 -1.58
Endoplasmic Reticulum 3.47 3.61 3.58 2.62 -1.01 -3.42 -4.69 6.51 8.72 6.70 1.38 1.82 3.76 5.63 4.33
Endosome 2.04 2.11 2.02 -0.34 -1.38 -2.73 -3.50 4.76 3.36 4.22 -0.87 -0.17 0.56 1.71 1.07
Golgi 4.99 5.42 5.50 10.30 2.36 5.55 6.97 10.26 7.29 3.84 6.11 9.17 10.65 11.27 4.57
Lipid Particles 5.24 5.84 5.88 4.29 0.51 -4.27 -6.59 -4.51 0.07 3.22 -3.94 -4.50 -2.35 2.42 3.08
Mitochondria 1.35 1.41 1.35 0.08 -2.06 -0.59 -4.02 4.44 1.97 6.14 0.73 1.25 1.44 1.16 0.74
Mitotic Spindle 0.77 1.30 1.02 0.86 -1.43 -2.84 -1.75 4.60 4.45 2.84 -2.70 0.09 0.16 2.35 0.10
None -7.14 -1.91 -1.15 6.56 0.52 1.69 4.72 8.10 6.75 1.17 0.77 9.04 9.14 9.54 0.81
Nuclear Periphery 4.40 4.78 4.80 6.63 0.73 4.32 -11.37 4.39 -0.11 15.94 2.86 0.60 5.29 5.21 12.01
Nuclear Periphery Foci 1.32 2.88 3.09 11.00 5.41 -4.72 -0.80 3.39 8.90 4.71 -6.51 1.78 4.79 14.15 6.97
Nucleolus 1.66 1.77 1.70 0.50 -5.34 -10.04 -5.05 -1.58 9.82 4.75 -0.32 0.41 0.59 1.41 0.75
Nucleus -6.81 -3.68 -1.27 14.56 9.20 7.60 -13.70 13.07 7.25 22.07 4.81 -1.71 15.66 15.87 20.43
Peroxisomes 1.26 0.95 1.08 -2.92 1.38 -4.89 -3.00 -4.41 3.02 -1.50 -4.02 -4.11 -3.89 1.72 1.50
Vacuole 7.96 7.01 3.21 -21.56 -18.32 -6.42 -24.19 -20.68 -17.30 10.34 -4.70 -23.13 -29.20 -29.14 -7.40
Vacuole Periphery 4.68 4.72 4.56 -1.16 -1.60 2.02 0.89 2.66 2.95 1.30 3.94 3.81 3.17 -0.74 -0.67
Standard name
Human Ortholog
Description 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; abundance relative to Bmh1p increases during sporulation
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Bmh2

Bmh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bmh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available