Standard name
Human Ortholog
Description Vacuolar membrane protein of unknown function; is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.09 0.17 0.2 0.25 0.38 0.35 0.44 0.37 0 0 0 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.07 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0.06 0.17 0.16 0.13 0 0.07 0.09 0 0 0 0 0.24 0.32 0.24 – – – 0.24 0.17 0.33 0.32 0.25 0.22
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.19 0 0.21 0.34 0.22 0.33 0.4 0.31 0.38 0.45 0.5 0.45 0.07 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0.14 0.09 0 0.05 0.11 0 0 0.05 0.05 0 0.05 0 0 – – – 0 0 0.07 0.06 0.06 0.06
Nucleolus 0.37 0.13 0.29 0.24 0.06 0.06 0 0 0 0 0 0 0.19 0.25 0.17 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.7 0.85 0.67 0.58 0.74 0.55 0.49 0.46 0.38 0.24 0.14 0.26 0.78 0.7 0.77 – – – 0.69 0.71 0.55 0.53 0.61 0.59
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 2 1 2 1 1 1 2 – – – 0 0 0 0 1 4
Bud 0 0 1 0 0 1 1 11 0 2 0 1 1 0 0 – – – 0 1 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 2 5 5 5 4 6 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 12 3 4 13 31 48 73 57 80 65 60 9 11 17 – – – 0 3 1 1 1 1
Endoplasmic Reticulum 0 1 0 0 0 6 5 7 7 17 9 11 8 8 13 – – – 0 0 0 0 0 1
Endosome 2 17 22 18 18 8 16 28 0 4 0 7 69 111 92 – – – 20 52 44 80 61 71
Golgi 0 7 2 4 4 2 1 1 0 0 2 1 4 4 12 – – – 0 3 0 0 1 11
Mitochondria 16 10 27 40 31 58 99 92 57 102 74 73 21 15 13 – – – 0 4 2 2 2 7
Nucleus 3 12 3 0 1 6 6 7 3 2 1 3 2 4 1 – – – 0 6 1 7 6 5
Nuclear Periphery 1 4 18 11 3 9 27 7 4 12 8 5 13 10 14 – – – 1 3 9 15 15 18
Nucleolus 31 37 37 28 9 10 11 5 1 3 2 3 53 87 67 – – – 3 7 0 5 3 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 1 1 4 0 0 0 0 3 3 2 – – – 0 0 0 1 1 2
Vac/Vac Membrane 58 242 85 67 106 98 121 136 58 56 20 42 221 245 300 – – – 59 215 74 132 150 193
Unique Cell Count 83 285 126 116 143 178 246 295 152 229 147 162 283 351 391 86 302 135 250 248 327
Labelled Cell Count 111 342 199 173 185 230 338 377 194 285 187 213 405 499 533 86 302 135 250 248 327


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 12.5 8.3 7.9 7.6 6.9 6.2 6.4 6.7 5.9 5.9 6.1 11.2 11.7 12.0 – – – 8.7 9.3 10.3
Std Deviation (1e-4) 1.5 2.5 2.1 1.5 1.6 1.9 1.7 1.7 1.6 1.4 1.5 1.4 2.8 3.1 3.5 – – – 2.7 3.0 3.0
Intensity Change (Log2) – – – -0.07 -0.14 -0.27 -0.44 -0.38 -0.32 -0.49 -0.49 -0.45 0.42 0.48 0.52 – – – 0.05 0.16 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0 2.3 4.1 4.5 5.5 7.1 6.9 8.0 7.1 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 3.1 0 0 0 0 0 – – –
Endosome -0.4 -1.1 -3.7 -3.3 -2.3 -5.4 -5.4 -5.3 -3.7 1.6 3.0 1.4 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 2.3 0 2.1 3.6 2.0 2.9 4.3 4.9 4.2 -4.1 -5.8 -6.6 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery -1.1 -3.7 -2.8 -0.9 -4.7 -3.6 -2.9 -2.5 -3.5 -3.4 -4.7 -4.3 – – –
Nucleolus -0.9 -5.0 -5.6 -6.8 -8.7 -6.9 -8.0 -6.6 -6.7 -2.4 -1.0 -3.0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole -1.6 1.2 -2.2 -3.4 -4.0 -4.9 -7.9 -9.1 -7.0 2.3 0.5 2.1 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.3559 7.3009 7.0914 7.4348 5.666 7.0386 5.8946 7.2445 6.5469 5.6584 5.4428 6.4229 7.4873 8.1869 8.2233 7.8662 7.5198 8.069
Actin 0.0246 0 0.0129 0 0.0176 0.0049 0.033 0 0.0017 0.0648 0 0 0.0592 0 0.0053 0.0049 0.0009 0.0008
Bud 0.0003 0.0005 0.0008 0 0.0007 0.0002 0.0004 0.0002 0.0008 0.0001 0.0005 0.0002 0.0077 0.001 0.0006 0.0001 0.0083 0.0001
Bud Neck 0.0006 0.0007 0.0006 0 0.0007 0.0004 0.0005 0.0002 0.0005 0.0002 0.0006 0.0007 0.0019 0.0011 0.0004 0.0001 0.0005 0.0004
Bud Periphery 0.0006 0.0005 0.001 0 0.0015 0.0004 0.0007 0.0004 0.0009 0.0001 0.0025 0.0004 0.0035 0.0006 0.0017 0.0002 0.0091 0.0003
Bud Site 0.0006 0.0043 0.0028 0 0.0011 0.0004 0.0018 0.0006 0.0042 0.0002 0.0011 0.0004 0.0031 0.0051 0.0006 0.0001 0.0028 0.0002
Cell Periphery 0.0011 0.0009 0.0007 0.0001 0.0004 0.0004 0.0012 0.0008 0.0004 0.0001 0.0004 0.001 0.001 0.0018 0.0006 0.0001 0.001 0.0004
Cytoplasm 0.0028 0.016 0.002 0.0007 0.0029 0.006 0.0061 0.0221 0.004 0.0096 0.0023 0.0006 0.0071 0.011 0.0043 0.0081 0.0033 0.0018
Cytoplasmic Foci 0.0115 0.0231 0.0216 0.0018 0.0156 0.0186 0.0261 0.0106 0.0281 0.0214 0.0192 0.0108 0.0192 0.0171 0.0099 0.015 0.0088 0.0144
Eisosomes 0.0002 0 0.0001 0 0.0002 0 0.0003 0 0 0.0001 0 0 0.0011 0 0 0 0 0
Endoplasmic Reticulum 0.0054 0.0015 0.0023 0.0003 0.0042 0.0009 0.0066 0.0021 0.0009 0.0004 0.001 0.0008 0.0038 0.0019 0.0016 0.0012 0.0011 0.0009
Endosome 0.2959 0.2161 0.2615 0.1246 0.2325 0.2408 0.2659 0.2522 0.3656 0.3717 0.3966 0.3277 0.2893 0.2227 0.3175 0.3731 0.2509 0.3179
Golgi 0.0148 0.0071 0.0209 0.0037 0.0127 0.0146 0.0246 0.0103 0.025 0.0126 0.0314 0.0146 0.0234 0.0055 0.0158 0.0115 0.0247 0.0163
Lipid Particles 0.0382 0.0248 0.0342 0.0069 0.0111 0.0828 0.0959 0.026 0.0528 0.0151 0.0111 0.0394 0.0277 0.0216 0.016 0.029 0.0241 0.0428
Mitochondria 0.0056 0.0044 0.047 0.0004 0.1068 0.0139 0.0076 0.0085 0.0183 0.0005 0.0139 0.004 0.0246 0.0011 0.0192 0.0017 0.0246 0.0027
None 0.0046 0.0011 0.0005 0 0.0018 0.0006 0.0052 0.0111 0.0052 0.0613 0.0728 0.0001 0.0237 0.0006 0.0124 0.0093 0.0011 0.0002
Nuclear Periphery 0.0225 0.0073 0.0129 0.0023 0.0701 0.0132 0.01 0.0283 0.0032 0.0017 0.0024 0.0179 0.0104 0.0136 0.0058 0.0024 0.0309 0.0124
Nucleolus 0.0043 0.0053 0.002 0.0002 0.0104 0.0047 0.0029 0.0026 0.0031 0.0003 0.004 0.0038 0.003 0.0055 0.0018 0.0004 0.0123 0.0027
Nucleus 0.003 0.0099 0.002 0.0004 0.0318 0.005 0.0028 0.0062 0.0017 0.0003 0.0016 0.004 0.0061 0.0048 0.0017 0.0006 0.0051 0.0014
Peroxisomes 0.0026 0.0027 0.002 0.0001 0.01 0.002 0.0137 0.0008 0.0065 0.0018 0.0021 0.0007 0.0042 0.0006 0.0015 0.0266 0.0028 0.0056
Punctate Nuclear 0.0047 0.0004 0.001 0 0.0156 0.0021 0.0037 0.0005 0.0031 0.0002 0.0007 0.0002 0.0097 0.0003 0.0007 0.0002 0.0006 0.0004
Vacuole 0.3067 0.4234 0.3135 0.3868 0.2469 0.3214 0.293 0.3177 0.2691 0.2113 0.2245 0.3062 0.286 0.4657 0.351 0.2756 0.3126 0.3195
Vacuole Periphery 0.2496 0.2498 0.2577 0.4715 0.2052 0.2668 0.1981 0.2986 0.2047 0.2263 0.2113 0.2667 0.1842 0.2182 0.2316 0.2396 0.2746 0.2585

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.3387 22.8906 12.868 21.5412 20.1211 25.5923 24.898 24.3783 25.6977 30.0738
Translational Efficiency 0.9607 0.592 1.1654 0.6661 0.7477 0.8422 0.6331 0.6306 0.7083 0.5937

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1914 1126 192 326 439 1681 182 193 2353 2807 374 519

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 946.67 927.10 1314.34 894.95 1094.70 1015.05 1330.68 992.81 974.29 979.77 1322.29 931.34
Standard Deviation 161.10 200.37 217.54 129.76 139.29 169.25 191.17 173.64 167.50 187.40 205.29 155.00
Intensity Change Log 2 — -0.030137 0.473405 -0.081055 — -0.108985 0.281628 -0.140946 — -0.071883 0.373741 -0.112862

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000037 0.000132 0.000244 0.000049 0.000053 0.000055 0.000192 0.000489 0.000040 0.000086 0.000219 0.000212
Bud Neck 0.000705 0.002350 0.014280 0.016397 0.000661 0.001322 0.015458 0.010198 0.000697 0.001735 0.014853 0.014091
Bud Site 0.005386 0.016108 0.005995 0.029506 0.002485 0.003545 0.010280 0.022274 0.004845 0.008585 0.008080 0.026817
Cell Periphery 0.000432 0.000584 0.000330 0.000121 0.000161 0.000252 0.000213 0.000177 0.000382 0.000385 0.000273 0.000142
Cytoplasm 0.002446 0.015602 0.000820 0.013006 0.000378 0.001437 0.002296 0.033538 0.002060 0.007119 0.001538 0.020641
Cytoplasmic Foci 0.005967 0.023069 0.009736 0.039396 0.022202 0.009869 0.064486 0.101572 0.008996 0.015164 0.036379 0.062517
Eisosomes 0.000007 0.000016 0.000084 0.000010 0.000007 0.000014 0.000039 0.000034 0.000007 0.000015 0.000062 0.000019
Endoplasmic Reticulum 0.000879 0.001127 0.001628 0.000618 0.000332 0.000879 0.000509 0.000479 0.000777 0.000979 0.001083 0.000566
Endosome 0.058515 0.123630 0.060645 0.207667* 0.118190 0.083490 0.175303 0.431159* 0.069649 0.099592 0.116441 0.290777*
Golgi 0.015679 0.054348 0.006790 0.014892 0.055605 0.020807 0.024070 0.047923 0.023128 0.034262 0.015199 0.027175
Lipid Particles 0.000558 0.002421 0.002388 0.001059 0.000856 0.001705 0.004420 0.004423 0.000614 0.001992 0.003377 0.002310
Mitochondria 0.010595 0.013072 0.007695 0.001445 0.030464 0.019127 0.006153 0.006273 0.014302 0.016698 0.006944 0.003240
Mitotic Spindle 0.000612 0.002851 0.001969 0.016884 0.001507 0.001910 0.014637 0.036017 0.000779 0.002287 0.008134 0.023999
None 0.000406 0.001800 0.000300 0.000085 0.000184 0.000730 0.000157 0.003634 0.000364 0.001159 0.000230 0.001405
Nuclear Periphery 0.000369 0.000519 0.004425 0.001898 0.001350 0.002368 0.004141 0.000598 0.000552 0.001626 0.004287 0.001415
Nuclear Periphery Foci 0.001130 0.003105 0.006882 0.003498 0.002925 0.004821 0.008533 0.003060 0.001465 0.004133 0.007685 0.003335
Nucleolus 0.001138 0.002180 0.032312 0.001866 0.000282 0.004163 0.014645 0.002284 0.000978 0.003368 0.023715 0.002021
Nucleus 0.000759 0.008364 0.020411 0.004637 0.000331 0.002671 0.005816 0.006950 0.000679 0.004954 0.013309 0.005497
Peroxisomes 0.000519 0.002327 0.006876 0.004178 0.001060 0.001271 0.016810 0.017690 0.000620 0.001695 0.011710 0.009202
Vacuole 0.322757* 0.345496* 0.465006* 0.487967* 0.256965* 0.364430* 0.451124* 0.233777* 0.310482* 0.356835* 0.458250* 0.393442*
Vacuole Periphery 0.571104* 0.380901* 0.351184* 0.154823* 0.504002* 0.475133* 0.180720* 0.037452 0.558585* 0.437333* 0.268231* 0.111177*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.06 -3.07 -1.20 3.74 2.77 -0.15 -1.81 -2.09 -2.08 -1.30 -3.76 -3.51 -2.26 -1.65 0.12
Bud Neck -4.47 -5.43 -6.64 -5.90 -0.73 -2.81 -5.71 -6.19 -5.72 1.91 -5.07 -7.87 -8.31 -7.64 0.29
Bud Site -5.24 -0.43 -6.02 -3.30 -5.72 -0.97 -4.14 -4.92 -4.74 -2.70 -3.51 -2.70 -7.42 -6.14 -6.11
Cell Periphery -2.09 1.43 5.43 7.73 3.54 -2.71 -1.72 -0.61 0.91 0.32 -0.09 2.12 4.75 6.15 3.13
Cytoplasm -5.59 1.34 -4.77 -0.54 -5.10 -3.09 -2.13 -4.73 -4.62 -4.50 -4.84 0.21 -6.26 -4.99 -6.28
Cytoplasmic Foci -9.78 -1.56 -7.00 -3.28 -5.65 4.22 -4.74 -7.99 -9.68 -2.67 -5.69 -5.98 -11.07 -9.72 -3.84
Eisosomes -6.48 -3.98 -3.06 3.61 3.80 -5.54 -7.05 -5.12 -3.75 0.70 -7.70 -5.39 -5.61 -2.08 4.11
Endoplasmic Reticulum -1.72 -1.81 1.08 2.25 2.14 -6.07 -1.95 -1.89 3.12 0.02 -2.15 -1.44 1.24 2.92 1.96
Endosome -10.61 -0.19 -11.97 -6.16 -9.45 3.69 -3.33 -13.05 -15.63 -9.22 -6.91 -4.84 -17.77 -15.17 -11.12
Golgi -11.61 5.42 0.52 10.42 -3.00 6.08 5.13 1.36 -3.58 -2.92 -5.24 3.81 -0.72 2.74 -3.22
Lipid Particles -2.92 -2.47 -1.29 1.83 1.61 -2.56 -2.84 -2.35 -1.75 -0.06 -4.36 -3.85 -2.74 -0.47 1.08
Mitochondria -1.20 1.15 7.42 6.61 2.71 2.49 5.06 4.84 3.71 0.07 -1.32 3.55 6.69 7.68 1.89
Mitotic Spindle -3.54 -1.57 -3.85 -3.33 -3.49 -0.61 -2.21 -4.49 -4.45 -2.44 -3.28 -2.49 -5.92 -5.57 -3.45
None -3.54 -0.24 3.19 4.45 1.71 -1.82 0.89 -1.12 -0.94 -1.13 -3.18 0.36 -0.92 -0.24 -0.96
Nuclear Periphery -2.03 -3.45 -2.82 -2.53 1.85 -2.42 -2.14 2.01 4.49 2.66 -5.25 -4.29 -2.58 0.24 2.94
Nuclear Periphery Foci -2.08 -3.83 -1.84 -0.41 1.56 -1.93 -3.16 -0.55 1.35 2.84 -4.06 -5.40 -2.25 0.45 2.87
Nucleolus -1.93 -6.21 -2.11 -0.16 5.94 -6.04 -4.47 -2.82 1.84 3.74 -5.01 -7.46 -2.92 1.73 6.96
Nucleus -3.90 -3.97 -2.31 0.98 2.88 -5.38 -3.84 -3.19 -2.37 -1.10 -5.09 -4.68 -3.74 -1.09 2.21
Peroxisomes -4.56 -5.49 -5.67 -2.57 1.92 -0.86 -4.18 -6.56 -6.47 0.01 -5.19 -5.72 -8.19 -7.03 1.26
Vacuole -2.88 -7.74 -11.20 -9.37 -1.14 -10.78 -10.28 0.63 6.38 8.34 -8.04 -11.41 -6.75 -3.20 3.42
Vacuole Periphery 18.81* 10.82 35.75* 17.92 9.12 1.93 17.87 32.88* 56.25* 11.33 15.94 21.42 50.50 38.17* 11.28
Standard name
Human Ortholog
Description Vacuolar membrane protein of unknown function; is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
Localization
Cell Percentages vacuolar membrane (85%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Tms1

Tms1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tms1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available