Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 – – – – – –
Cell Periphery 0 0.06 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.26 0.11 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.24 0.13 0 0 0 0 0.05 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0.08 0.06 0 – 0 0 0 0 0.05 0.05 0.05 0 0 0.08 0.05 0 0 0 0.06 0
Golgi 0 0 0.26 0.11 0.17 0 0 – 0 0 0 0 0.13 0.32 0.24 0 0.27 0.16 0.07 0.06 0.05 0.07 0.09 0.07
Mitochondria 0.91 0.92 0.73 0.9 0.71 0.78 0.87 – 0.9 0.9 0.9 0.92 0.82 0.81 0.76 0.05 0.08 0.08 0.79 0.79 0.78 0.72 0.59 0.65
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.05 0.05 0 0 0 0 0 0
Nuclear Periphery 0.08 0 0 0 0 0.07 0.06 – 0.09 0.06 0.08 0.09 0.06 0 0 0.05 0.05 0.09 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0.06 0.07 0.08 – 0.08 0.08 0.07 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0 0.1 0 0 – 0 0 0 0 0.06 0 0.06 0.23 0 0.08 0 0.05 0.07 0 0.05 0.06
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.13 0.07 0 0.09 0.11 0.08 – 0 0 0 0 0.09 0.09 0.1 0.16 0.05 0.21 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 6 3 1 0 0 – 1 0 1 1 2 1 0 0 1 0 2 4 4 2 6 6
Bud 0 5 1 6 7 4 12 – 5 13 13 18 1 2 0 1 4 3 0 2 1 0 3 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 1 2 0 0 0 1 1 – 2 2 2 8 0 0 0 3 0 1 – – – – – –
Cell Periphery 2 21 18 5 7 5 3 – 0 2 6 7 2 6 6 11 4 5 0 0 1 2 0 0
Cytoplasm 1 2 8 12 11 18 14 – 15 8 10 6 5 6 7 2 9 13 0 0 1 6 11 11
Endoplasmic Reticulum 1 0 1 2 3 2 0 – 0 0 3 1 0 0 2 2 1 3 0 0 0 2 2 0
Endosome 5 10 22 8 26 24 6 – 4 4 8 7 8 9 10 1 1 8 10 15 17 4 14 9
Golgi 9 3 88 31 57 13 1 – 2 2 5 7 20 61 46 1 10 16 16 23 19 13 21 18
Mitochondria 218 343 250 255 241 331 458 – 337 420 409 485 124 156 143 2 3 8 191 307 313 135 142 159
Nucleus 2 3 1 0 0 3 9 – 2 4 2 1 0 1 2 5 2 5 0 0 0 1 3 1
Nuclear Periphery 19 12 11 10 11 28 32 – 34 30 36 46 9 5 4 2 2 9 2 1 2 4 1 1
Nucleolus 11 8 2 10 20 31 43 – 31 37 32 39 3 4 2 1 0 5 1 0 1 0 3 2
Peroxisomes 6 11 27 6 33 6 0 – 3 4 8 6 9 8 12 10 1 8 9 17 29 7 13 13
SpindlePole 7 3 10 5 15 9 5 – 4 9 5 7 3 1 2 4 1 9 1 2 0 1 1 4
Vac/Vac Membrane 37 47 25 11 32 45 41 – 12 15 18 12 13 17 18 7 2 20 4 11 2 4 9 8
Unique Cell Count 239 374 342 284 341 423 527 374 466 452 526 151 193 188 43 37 97 243 388 403 189 240 245
Labelled Cell Count 320 471 470 364 464 520 625 452 550 558 651 199 277 254 52 41 113 243 388 403 189 240 245


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 7.0 8.9 8.7 9.4 8.4 5.7 – 7.5 7.3 7.6 7.6 9.5 9.1 8.4 5.5 8.3 7.5 11.6 11.8 11.8
Std Deviation (1e-4) 1.3 1.3 1.7 1.7 1.7 1.7 1.6 – 1.9 1.6 1.7 1.6 3.1 2.6 1.4 1.5 2.0 1.7 8.2 8.1 8.3
Intensity Change (Log2) – – – -0.04 0.08 -0.08 -0.65 – -0.24 -0.28 -0.23 -0.22 0.09 0.02 -0.08 -0.68 -0.1 -0.24 0.38 0.41 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2.4 – 0 2.7 2.7 3.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.3 -2.2 -3.3 -4.4 – -4.5 -4.4 -3.2 -3.4 -2.0 -1.2 -1.1 0 0 0
Cytoplasm 1.3 0.7 1.5 0.3 – 1.3 -0.6 -0.1 -1.4 0 0.5 0.9 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -2.1 0.6 -0.4 -4.3 – -3.8 -4.4 -3.4 -4.1 -0.5 -0.8 -0.5 0 0 0.6
Golgi -4.7 -2.9 -9.2 -12.1 – -10.2 -11.3 -10.7 -11.3 -3.1 1.5 -0.3 -3.4 0.2 -1.9
Mitochondria 5.3 -0.7 1.7 5.1 – 5.9 6.4 6.5 7.6 2.2 2.0 0.7 -8.9 -8.0 -11.5
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.2 0 2.1 1.9 – 3.2 2.1 2.8 3.2 1.4 -0.4 -0.7 0 0 0
Nucleolus 2.7 3.9 4.6 4.9 – 4.9 4.8 4.5 4.6 0 0 0 0 0 0
Peroxisomes -3.2 0.8 -4.4 -6.6 – -4.7 -5.1 -4.2 -5.1 -0.8 -1.7 -0.6 0 0 0.1
SpindlePole -0.9 1.0 -0.7 -2.2 – -1.8 -0.9 -1.9 -1.7 0 0 0 0 0 0
Vacuole -1.8 1.0 1.6 0.3 – -2.5 -2.6 -2.1 -3.6 0.5 0.6 0.9 0 0 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.0954 11.451 11.1133 10.2655 9.9378 11.0815 9.9016 12.4127 10.9308 11.1134 9.5374 11.412 8.4337 8.133 8.1728 8.4617 7.8558 8.2669
Actin 0.0699 0.0068 0.038 0.0271 0.0264 0.0094 0.0329 0.0177 0.0144 0.0275 0.0515 0.0059 0.0361 0.0032 0.0116 0.0018 0.0026 0.01
Bud 0.0003 0.0022 0.0005 0.0007 0.0003 0.0003 0.0006 0.0018 0.0005 0.001 0.0009 0 0.0002 0.0005 0.0002 0.0036 0 0.0002
Bud Neck 0.0021 0.0002 0.0003 0.0002 0.0002 0.0002 0.0008 0.0023 0.0024 0.0001 0.0004 0.0001 0.0004 0 0.0003 0.0092 0 0.0016
Bud Periphery 0.0006 0.0028 0.0008 0.0013 0.0007 0.0006 0.0009 0.001 0.0007 0.0023 0.0016 0.0001 0.0004 0.0015 0.0007 0.016 0.0001 0.0004
Bud Site 0.0026 0.0062 0.0016 0.0008 0.0011 0.0006 0.0066 0.0165 0.0033 0.0008 0.0046 0.0001 0.0005 0.0005 0.001 0.0024 0 0.0003
Cell Periphery 0.0003 0.0004 0.0003 0.0003 0.0002 0.0001 0.0004 0.0022 0.0002 0.0002 0.0003 0.0001 0.0004 0.0001 0.0003 0.0071 0 0.0001
Cytoplasm 0.0167 0.0001 0.0015 0.0002 0.0001 0.0001 0.0079 0.0001 0.0014 0.0003 0.0003 0 0.0121 0 0.001 0.001 0 0.0002
Cytoplasmic Foci 0.02 0.0025 0.0116 0.0025 0.0048 0.0025 0.0342 0.0105 0.0112 0.0013 0.0194 0.0014 0.0108 0.0015 0.004 0.0021 0.0003 0.0021
Eisosomes 0.0008 0.0002 0.0004 0.0003 0.0007 0.0002 0.0004 0.0004 0.0003 0.0003 0.0009 0.0001 0.0004 0.0002 0.0004 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.0042 0.0001 0.0014 0.0006 0.0002 0.0002 0.0027 0.0001 0.0004 0.0009 0.0004 0.0001 0.0129 0.0001 0.0011 0.0001 0 0.0003
Endosome 0.0222 0.0076 0.0228 0.0182 0.0051 0.0047 0.022 0.0114 0.0081 0.0064 0.0184 0.0022 0.0188 0.0095 0.0104 0.0073 0.0004 0.008
Golgi 0.0143 0.0326 0.0256 0.0466 0.0157 0.0172 0.0103 0.0093 0.0116 0.007 0.0199 0.0125 0.0246 0.0234 0.0141 0.0089 0.0017 0.0175
Lipid Particles 0.0425 0.0165 0.0125 0.0117 0.0347 0.013 0.0431 0.0148 0.0073 0.0018 0.0413 0.0045 0.0193 0.0076 0.0066 0.002 0.012 0.0059
Mitochondria 0.7412 0.9112 0.868 0.8515 0.8542 0.9266 0.7462 0.8231 0.9099 0.9452 0.779 0.9531 0.8161 0.9383 0.9296 0.9327 0.9771 0.9436
None 0.0085 0.0001 0.0004 0.0001 0.0005 0.0001 0.024 0.0007 0.0005 0.0006 0.0008 0 0.0045 0 0.0016 0.0001 0 0.0001
Nuclear Periphery 0.0091 0.0001 0.0009 0.0007 0.0035 0.0003 0.0059 0.0003 0.0002 0.0004 0.0024 0.0002 0.0073 0.0001 0.0023 0 0 0.0011
Nucleolus 0.0007 0.0002 0.0001 0.0002 0.0034 0.0001 0.0078 0.0063 0.0004 0.0001 0.0007 0 0.0004 0.0001 0.0003 0.0001 0.0002 0.0001
Nucleus 0.0025 0.0001 0.0002 0.0003 0.0019 0.0001 0.0138 0.0007 0.0001 0.0001 0.0004 0 0.0013 0 0.0004 0.0001 0 0.0003
Peroxisomes 0.0264 0.0057 0.0086 0.0038 0.0323 0.0203 0.0195 0.036 0.0197 0.0019 0.0489 0.0162 0.026 0.0077 0.0065 0.0025 0.0046 0.0038
Punctate Nuclear 0.0099 0 0.0011 0.0001 0.009 0.0001 0.0067 0.0071 0.0004 0.0001 0.0018 0 0.0038 0 0.001 0 0 0.0007
Vacuole 0.0018 0.002 0.0014 0.0103 0.0007 0.0005 0.0068 0.013 0.0026 0.0006 0.002 0.0005 0.0013 0.0013 0.0028 0.0019 0.0001 0.0006
Vacuole Periphery 0.0036 0.0025 0.0021 0.0225 0.0042 0.0027 0.0067 0.0246 0.0046 0.001 0.0043 0.0028 0.0025 0.0043 0.0037 0.0009 0.0006 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.5053 18.394 20.4737 24.5517 20.8423 21.8502 23.8288 27.4585 24.3663 29.5364
Translational Efficiency 1.2238 1.0576 1.2442 1.202 1.3235 1.5908 1.4167 1.5083 1.8596 1.609

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl1

Mrpl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available