Standard name
Human Ortholog
Description Protein of unknown function; proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.08 0.07 0.06 0.13 0.09 0.18 0.15 0 0.07 0 0.09 0.11 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0.05 0.07 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.05 0.12 0 0 0 0 0 0 0 0.05 0 0 0 0 0.08 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.18 0.12 0.3 0.3 0.35 0.45 0.41 0.44 0.45 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0.09 0.08 0.05 0.05 0 0.08 0.07 0.09
Nuclear Periphery 0.09 0.1 0.29 0.3 0.15 0.24 0.27 0.16 0.13 0.17 0.09 0.09 0.19 0.19 0.2 0.33 0.37 0.3 0.05 0.05 0.16 0.08 0.07 0.06
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.82 0.83 0.83 0.82 0.79 0.54 0.57 0.53 0.44 0.47 0.41 0.44 0.9 0.84 0.9 0.78 0.71 0.77 0.84 0.86 0.77 0.75 0.76 0.72
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 1 1 5 3 1 1 2 0 0 0 0 0 0 0 0 0 0 2 1
Bud 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 0 0 0 1 3 2 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 3 6 20 18 20 17 11 14 1 0 0 2 1 9 0 0 0 0 0 0
Cytoplasm 12 17 17 8 18 35 40 38 64 50 54 62 2 9 8 37 33 23 0 2 0 0 0 0
Endoplasmic Reticulum 0 1 1 0 3 16 16 38 24 39 24 23 0 0 0 8 6 5 0 0 1 0 1 0
Endosome 0 2 2 3 1 1 1 1 2 0 1 0 0 0 0 0 1 0 1 1 1 2 3 5
Golgi 12 3 8 15 48 13 12 21 11 10 3 2 5 1 6 4 2 18 1 0 1 0 2 0
Mitochondria 3 12 18 50 50 131 166 215 225 226 131 183 1 0 0 19 13 4 2 5 13 3 3 4
Nucleus 26 37 4 1 12 15 5 7 7 8 4 3 3 9 9 30 28 18 15 20 2 10 17 17
Nuclear Periphery 25 40 108 86 61 105 145 95 66 94 26 36 19 26 35 140 112 70 15 21 61 10 17 11
Nucleolus 10 5 14 7 9 9 6 5 5 0 2 2 0 1 0 6 7 4 5 3 4 4 8 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 0
Vac/Vac Membrane 225 317 314 231 320 235 311 325 220 258 124 180 91 114 161 325 216 182 240 355 297 103 198 133
Unique Cell Count 274 383 378 282 407 433 546 608 502 551 299 405 101 136 179 418 303 235 286 414 388 137 262 186
Labelled Cell Count 313 435 487 402 525 567 723 770 648 703 382 507 122 160 219 572 419 334 286 414 388 137 262 186


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.4 22.9 22.3 19.5 16.2 13.0 11.5 11.3 10.6 9.5 9.6 9.4 18.3 16.7 15.4 12.0 12.3 16.5 18.5 19.4 20.7
Std Deviation (1e-4) 5.9 6.2 5.3 4.1 3.9 3.6 3.2 2.8 2.6 2.5 2.5 2.3 4.6 4.3 3.6 3.5 4.5 5.6 5.4 5.9 6.6
Intensity Change (Log2) – – – -0.2 -0.46 -0.78 -0.95 -0.98 -1.08 -1.23 -1.23 -1.25 -0.29 -0.42 -0.54 -0.89 -0.86 -0.44 -0.27 -0.2 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.4 3.0 3.6 3.1 3.3 3.3 0 0 0 0 0 0
Cytoplasm -1.1 -0.1 2.1 1.8 1.2 4.2 2.6 5.7 5.0 0 1.0 0 2.4 3.2 2.6
Endoplasmic Reticulum 0 0 3.4 3.0 4.7 4.0 5.0 5.3 4.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.2 5.3 0.8 0.1 1.2 0.1 -0.3 0 0 0 0 0 -1.3 0 3.3
Mitochondria 5.4 3.7 9.4 9.6 11.0 13.2 12.3 12.2 12.9 0 0 -3.0 -0.1 -0.3 -2.0
Nucleus 0 1.9 2.3 0 0 0 0 0 0 0 0 0 4.3 5.0 4.3
Nuclear Periphery 0.5 -4.6 -1.4 -0.7 -4.9 -5.7 -4.2 -6.4 -7.1 -2.0 -2.2 -2.3 1.5 2.3 0.3
Nucleolus -0.9 -1.2 -1.4 -2.7 -3.2 -2.7 -4.6 -2.6 -3.2 0 0 0 -2.0 -1.0 -1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.4 -1.6 -8.7 -8.3 -9.5 -11.8 -11.2 -11.2 -11.2 1.7 0.2 2.1 -1.9 -3.7 -1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.5503 14.1061 13.8415 10.8621 10.4671 12.0007 13.2945 15.158 14.0135 13.0574 13.4781 13.5814 15.6927 17.2807 16.8459 16.7756 15.041 16.0554
Actin 0.001 0 0.0044 0.0033 0.0033 0 0.0002 0 0.0018 0.0161 0 0 0.0037 0 0.0004 0 0 0
Bud 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0003 0.0007 0 0 0.0001 0 0.0001 0 0 0
Bud Neck 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0.0001 0 0 0.0001 0 0 0 0 0
Bud Periphery 0.0002 0 0.0004 0.0002 0.0006 0.0002 0.0002 0.0001 0.0011 0.0029 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Site 0.0004 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0003 0.0004 0 0 0.0001 0 0 0 0 0
Cell Periphery 0.0009 0.0003 0.0007 0.0003 0.0007 0.0003 0.0006 0.0006 0.0008 0.0003 0.0002 0.0002 0.0005 0.0004 0.0005 0.0002 0.0002 0.0002
Cytoplasm 0.0014 0.0001 0.0001 0 0.0004 0.0001 0.0001 0 0.0002 0.0001 0 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasmic Foci 0.0018 0 0.0005 0.0006 0.0004 0.0001 0.004 0 0.0017 0.0002 0 0 0.0001 0 0.0009 0 0.0001 0
Eisosomes 0 0 0 0.0001 0 0 0 0 0 0.0002 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0011 0.0001 0.0001 0.0001 0.0015 0.0001 0.0003 0.0003 0.0006 0.0017 0.0001 0.0002 0.0002 0.0003 0.0002 0 0.0001 0.0001
Endosome 0.0055 0.0018 0.003 0.0021 0.0073 0.0038 0.0081 0.0029 0.009 0.0062 0.0012 0.0035 0.0033 0.0016 0.0041 0.0016 0.0009 0.0017
Golgi 0.0004 0 0.0004 0.0006 0.0007 0.0001 0.0011 0 0.0016 0.0036 0 0.0001 0.0003 0 0.0004 0 0 0
Lipid Particles 0.0038 0.0001 0.0009 0.0035 0.0018 0.0005 0.004 0.0001 0.0024 0.0016 0.0002 0.0006 0.0006 0.0001 0.0009 0.0001 0.0006 0.0002
Mitochondria 0.005 0.0002 0.0038 0.0236 0.0025 0.0018 0.0159 0.0009 0.0137 0.0036 0.018 0.0006 0.0054 0.0002 0.009 0.0002 0.0002 0.0003
None 0.0008 0 0.0001 0.0001 0.0006 0 0.0001 0 0.0004 0.0002 0 0 0.0001 0 0 0 0 0
Nuclear Periphery 0.0202 0.0175 0.0084 0.003 0.0266 0.0115 0.0136 0.0125 0.0132 0.0037 0.0024 0.009 0.0061 0.0078 0.0111 0.0007 0.0029 0.0093
Nucleolus 0.0025 0.0001 0.0004 0.0001 0.0018 0.0003 0.0009 0.0001 0.0004 0.0001 0.0001 0.0002 0.0006 0.0001 0.0002 0.0001 0.0192 0.0002
Nucleus 0.0043 0.0008 0.0005 0.0002 0.0042 0.0006 0.003 0.0004 0.001 0.0004 0.0002 0.0003 0.0007 0.0003 0.0004 0.0002 0.0051 0.0002
Peroxisomes 0.0002 0 0.0005 0.0018 0.0001 0 0.0012 0 0.003 0.0002 0 0 0.0001 0 0.0031 0 0 0
Punctate Nuclear 0.0017 0 0.0003 0 0.0013 0 0.0007 0 0.0011 0.0002 0 0 0.0001 0 0.0001 0 0.0004 0
Vacuole 0.2414 0.2418 0.2548 0.2108 0.2325 0.1995 0.2058 0.2155 0.2142 0.2195 0.171 0.1359 0.2118 0.2002 0.2045 0.2167 0.129 0.1365
Vacuole Periphery 0.7073 0.7371 0.7204 0.7492 0.7133 0.7808 0.7398 0.7665 0.7331 0.7379 0.8065 0.8491 0.766 0.7888 0.7637 0.7802 0.8412 0.8513

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.2205 42.7558 54.5698 65.4644 63.252 36.8076 44.8515 52.5196 51.4789 73.131
Translational Efficiency 0.9735 1.1953 0.9528 0.6802 0.9294 1.3085 0.9373 0.7669 0.8177 0.8457

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
Localization
Cell Percentages vacuolar membrane (90%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Vba4

Vba4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vba4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available