Standard name
Human Ortholog
Description Putative mannose-ethanolamine phosphate phosphodiesterase; involved in GPI-anchor remodeling prior to the attachment of cell wall proteins to beta 1,3-glucan, removing ethanolamine phosphate from the first mannose of GPI anchors; mutants display elevated Ca2+-dependent signaling resulting in secondary actin polarization and Golgi inheritance defects; enzyme is Mn2+-dependent; mutants have cell division cycle defect and fragile cell walls
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.98 0.99 0.95 0.93 0.9 0.86 0.88 0.81 0.8 0.69 0.76 1.0 1.0 0.99 0.87 0.91 0.9 0.68 0.78 0.67 0.88 0.81 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0 0 0.08 0.07 0.26 0.3 0.18 0.32 0.37 0.53 0.47 0 0 0 0.05 0 0 0.14 0.07 0.16 0.05 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.11 0.1 0.07 0.05 0.05 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 1 1 0 2 8
Bud 0 0 1 2 4 3 0 8 6 6 9 3 1 2 0 0 0 0 1 0 1 2 8 15
Bud Neck 0 0 0 0 0 0 0 2 3 3 1 3 0 0 0 0 0 0 2 0 0 1 3 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 1 1 0 0 1 2 0 0 2 3 0 0 2 3 0 0 2 0 0 1 2
Cytoplasm 162 112 161 118 162 223 209 316 173 209 129 178 290 473 503 203 179 126 136 105 107 225 301 328
Endoplasmic Reticulum 0 0 0 1 0 0 1 1 1 0 1 0 0 0 2 13 18 3 1 0 3 0 1 3
Endosome 0 0 0 0 4 0 0 0 0 0 0 0 0 1 0 7 3 4 4 1 5 3 3 5
Golgi 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 1 1
Mitochondria 47 5 2 10 12 65 72 64 69 97 100 111 0 0 0 12 1 3 28 9 25 12 20 18
Nucleus 0 0 0 0 2 0 0 3 1 2 0 1 1 0 1 3 0 1 0 1 0 0 0 1
Nuclear Periphery 0 1 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 1 2 4 1 9 3 0 0 0 0 0 0 0 0 1 2 1 6 3
Vac/Vac Membrane 0 0 1 1 2 4 2 3 4 2 0 2 0 2 0 32 22 14 14 7 7 6 11 36
Unique Cell Count 183 114 163 124 174 247 243 361 213 261 188 235 290 475 506 233 196 140 201 135 162 257 371 438
Labelled Cell Count 210 118 166 133 187 297 286 405 260 332 243 302 295 479 508 273 226 151 201 135 162 257 371 438


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.0 3.8 3.5 4.0 3.1 2.8 3.3 3.0 2.9 2.8 2.6 4.1 3.9 4.0 5.1 6.5 5.8 3.8 4.2 4.9
Std Deviation (1e-4) 0.8 1.0 1.6 1.6 1.9 1.2 0.9 1.3 1.7 1.7 1.0 1.0 1.0 1.0 1.1 2.0 3.4 1.8 0.9 1.5 1.3
Intensity Change (Log2) – – – -0.09 0.1 -0.26 -0.44 -0.2 -0.31 -0.39 -0.42 -0.54 0.13 0.06 0.11 0.45 0.8 0.62 0.02 0.16 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.6 4.1 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8933 0.3667 0.2023 0.6264 0.1981 0.5349 1.6718 1.4027 1.569 1.2434 1.2951 1.5461 2.1413 2.4126 2.4902 2.7481 2.0697 2.0894
Actin 0.064 0.0004 0.0058 0.0216 0.0089 0.0235 0.0568 0.0169 0.0078 0.0036 0.0113 0.0023 0.045 0.0033 0.0218 0.0003 0.0019 0.0043
Bud 0.0019 0.006 0.0004 0.0005 0.0011 0.0044 0.0015 0.006 0.0037 0.0004 0.001 0.0003 0.0008 0.0013 0.0017 0.0005 0.0001 0.0002
Bud Neck 0.0068 0.0002 0.0046 0.0058 0.0007 0.002 0.0035 0.0005 0.001 0.0002 0.0023 0.0008 0.0123 0.0018 0.0005 0.0001 0.0005 0.0003
Bud Periphery 0.0066 0.0012 0.0007 0.0012 0.0009 0.0087 0.0037 0.0111 0.0036 0.0011 0.0014 0.0015 0.0021 0.0029 0.0037 0.0015 0.0001 0.0004
Bud Site 0.0116 0.0036 0.0019 0.0042 0.0208 0.0013 0.013 0.0149 0.0123 0.0003 0.0056 0.0001 0.0065 0.0077 0.0049 0.0001 0.0002 0.0002
Cell Periphery 0.0013 0.0006 0.0003 0.0005 0.0002 0.0002 0.001 0.001 0.001 0.0001 0.0002 0.0001 0.0004 0.0011 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.1171 0.2832 0.2191 0.2912 0.182 0.2894 0.0632 0.1687 0.0668 0.0914 0.0613 0.113 0.1005 0.1506 0.1098 0.1137 0.1794 0.1934
Cytoplasmic Foci 0.0621 0.0476 0.0705 0.0307 0.096 0.0377 0.0406 0.016 0.0382 0.0634 0.0666 0.04 0.0381 0.0253 0.0294 0.0299 0.0576 0.0261
Eisosomes 0.0011 0.0001 0.0004 0.0004 0.0001 0.0002 0.0011 0.0006 0.0006 0.0001 0.0002 0.0002 0.0005 0.0003 0.0005 0.0003 0.0003 0.0001
Endoplasmic Reticulum 0.0143 0.0073 0.0046 0.008 0.005 0.0033 0.0045 0.0021 0.002 0.0025 0.0026 0.0008 0.0042 0.0051 0.0033 0.0048 0.0021 0.0031
Endosome 0.0346 0.0323 0.0419 0.0389 0.1158 0.0199 0.0328 0.0105 0.0344 0.0338 0.0332 0.0052 0.0336 0.0167 0.0214 0.0826 0.018 0.0078
Golgi 0.0123 0.0017 0.0065 0.0083 0.0121 0.0031 0.0084 0.0018 0.0116 0.0118 0.0097 0.001 0.0051 0.0016 0.0033 0.0043 0.002 0.0009
Lipid Particles 0.0286 0.0083 0.0155 0.03 0.0392 0.0055 0.0204 0.0075 0.0066 0.0099 0.0232 0.003 0.0165 0.0029 0.0065 0.0008 0.0011 0.0024
Mitochondria 0.0073 0.0013 0.0024 0.0015 0.003 0.0028 0.0078 0.0033 0.0135 0.0022 0.0402 0.0027 0.0057 0.0018 0.0039 0.0059 0.001 0.0011
None 0.5638 0.59 0.6016 0.5395 0.4877 0.5741 0.692 0.7213 0.7821 0.7728 0.696 0.8117 0.6658 0.7671 0.7687 0.7441 0.6959 0.7532
Nuclear Periphery 0.0168 0.003 0.0018 0.0042 0.0029 0.0022 0.0062 0.0006 0.0007 0.0011 0.0007 0.0046 0.0085 0.002 0.0035 0.0018 0.0037 0.0005
Nucleolus 0.0015 0.0004 0.001 0.0005 0.001 0.0005 0.0024 0.0004 0.0008 0.0003 0.0006 0.0002 0.001 0.0005 0.0004 0.0002 0.002 0.0006
Nucleus 0.0041 0.0011 0.0016 0.0014 0.001 0.0012 0.0061 0.0009 0.0009 0.0005 0.0004 0.0014 0.0112 0.0024 0.003 0.0007 0.0053 0.001
Peroxisomes 0.022 0.0014 0.0075 0.0037 0.009 0.0043 0.0231 0.0096 0.007 0.0011 0.0382 0.0084 0.0261 0.0007 0.0069 0.0007 0.0164 0.0021
Punctate Nuclear 0.0158 0.0027 0.0045 0.0042 0.0045 0.0138 0.0063 0.0006 0.002 0.0012 0.0031 0.0014 0.0094 0.0013 0.0038 0.0003 0.0099 0.001
Vacuole 0.0044 0.0067 0.0064 0.003 0.0069 0.0014 0.004 0.0048 0.0029 0.0016 0.0018 0.001 0.0056 0.0033 0.0023 0.0051 0.0018 0.0011
Vacuole Periphery 0.0021 0.0009 0.0012 0.0008 0.0013 0.0005 0.0016 0.0007 0.0006 0.0006 0.0006 0.0004 0.001 0.0005 0.0006 0.0023 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.791 19.38 15.3726 31.4654 22.7774 26.1503 30.7441 31.9233 24.8766 37.7173
Translational Efficiency 1.6086 1.7274 1.9237 0.9368 1.3776 1.3854 1.217 0.8725 1.288 0.7869

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative mannose-ethanolamine phosphate phosphodiesterase; involved in GPI-anchor remodeling prior to the attachment of cell wall proteins to beta 1,3-glucan, removing ethanolamine phosphate from the first mannose of GPI anchors; mutants display elevated Ca2+-dependent signaling resulting in secondary actin polarization and Golgi inheritance defects; enzyme is Mn2+-dependent; mutants have cell division cycle defect and fragile cell walls
Localization
Cell Percentages cytoplasm (33%), vacuole (3%), mixed (42%)
Cell Cycle Regulation Yes
vacuole - MA (0.00025)
Subcompartmental Group N/A

Cdc1

Cdc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available