Standard name
Human Ortholog
Description Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Env10p/Snd2p and Pho88p/Snd3p; can compensate for loss of SRP; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cytoplasm

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.97 0.96 0.98 0.97 0.93 0.92 0.92 0.85 0.69 0.79 0.99 1.0 0.99 0.9 0.89 0.86 0.87 0.93 0.88 0.75 0.78 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0.07 0.08 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.05 0 0.17 0.13 0.09 0.32 0.48 0.29 0 0 0 0 0 0 0 0 0 0.07 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.13 0 0 0 0.05 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 2 0 1 0 0 1 0 0 0 0 0 0 2 0 0 1 0 0 1 4 1
Bud 0 0 3 0 0 0 1 3 5 4 6 2 3 0 0 0 0 0 0 1 0 6 2
Bud Neck 0 0 0 0 0 0 0 2 0 0 2 0 0 0 0 0 0 0 1 2 1 4 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 5 9 5 9 4 4 8 0 2 9 2 14 5 3 6 7 0 0 0 0 1 1
Cytoplasm 145 172 276 167 293 209 303 274 160 118 208 228 420 416 162 157 156 139 176 258 122 378 351
Endoplasmic Reticulum 2 7 5 1 1 0 2 0 1 1 2 0 0 1 10 5 12 4 1 5 11 38 25
Endosome 1 0 0 0 0 1 1 0 0 1 0 0 1 2 2 2 1 3 1 0 0 1 1
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 1 0 2 1
Mitochondria 10 1 0 9 6 39 43 26 60 82 77 10 4 6 5 3 8 2 2 12 11 17 11
Nucleus 2 0 3 1 1 1 3 6 0 7 3 0 0 1 4 4 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 1 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 3 0 3 2 3 0 3 4 0 1 0 0 1 2 14 15 23 2 3 5 8 25 23
Unique Cell Count 156 178 287 170 301 225 331 299 189 172 264 230 422 422 181 177 182 161 189 294 163 486 430
Labelled Cell Count 166 186 301 185 314 254 360 326 228 216 310 242 443 433 202 192 211 161 189 294 163 486 430


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 7.6 6.9 6.0 6.7 4.8 5.2 5.5 4.4 4.1 4.7 6.8 6.8 6.8 9.4 9.4 9.5 6.6 6.6 7.0
Std Deviation (1e-4) 0.9 1.1 1.2 0.9 1.7 1.1 1.4 1.5 1.3 1.1 1.2 1.2 1.0 1.4 1.4 1.6 1.5 1.5 1.3 1.5
Intensity Change (Log2) -0.21 -0.05 -0.52 -0.42 -0.33 -0.65 -0.77 -0.55 -0.02 -0.03 -0.02 0.44 0.44 0.47 -0.07 -0.06 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -0.1 0 -1.7 -0.3 0 0 0 0 0.1 -1.8 0 0.2 0.4
Cytoplasm 1.2 0.8 -1.6 -2.4 -2.3 -4.4 -8.2 -6.2 2.1 3.3 2.1 -2.9 -3.1 -4.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.3 0 2.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 6.3 5.1 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 3.8 4.0 5.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1812 4.4647 3.5351 3.4841 2.0809 3.6031 2.4584 3.0657 2.9142 2.3862 2.6228 2.8981 2.1851 2.8923 2.6754 2.8573 1.8461 2.5189
Actin 0.0137 0.0003 0.0138 0.0097 0.0002 0.0036 0.0205 0.001 0.0241 0.0174 0.0004 0.0033 0.0466 0.011 0.0164 0.0311 0.0054 0.0066
Bud 0.0001 0.0006 0.0014 0.0009 0.0005 0.0005 0.0015 0.0025 0.0168 0.0019 0.0006 0.0018 0.0053 0.0139 0.0123 0.0153 0.0075 0.0077
Bud Neck 0.0003 0.0001 0.0032 0.0018 0.0004 0.0014 0.0014 0.0007 0.0018 0.0025 0.0083 0.0018 0.0027 0.0005 0.0031 0.0019 0.0012 0.0015
Bud Periphery 0.0002 0.0009 0.0014 0.0008 0.0005 0.0008 0.0015 0.0016 0.0134 0.0021 0.0003 0.0018 0.007 0.0092 0.0123 0.0147 0.0047 0.0122
Bud Site 0.0009 0.0018 0.0177 0.0035 0.0003 0.0003 0.0115 0.0123 0.0135 0.0056 0.001 0.0009 0.0077 0.0117 0.0134 0.0035 0.0058 0.0012
Cell Periphery 0.0008 0.0007 0.0009 0.0004 0.0014 0.0003 0.0004 0.0004 0.0021 0.0029 0.0001 0.0002 0.0007 0.0008 0.0005 0.0006 0.0002 0.0004
Cytoplasm 0.5457 0.7225 0.6179 0.793 0.5957 0.73 0.6756 0.8789 0.8024 0.711 0.8531 0.8625 0.661 0.8394 0.6954 0.5671 0.7451 0.7937
Cytoplasmic Foci 0.0164 0.014 0.019 0.0621 0.0189 0.0298 0.0258 0.0259 0.0312 0.0526 0.0407 0.0435 0.04 0.0281 0.0293 0.0529 0.0447 0.0421
Eisosomes 0.0007 0.0001 0.0003 0 0.0005 0.0001 0.0002 0 0.0001 0.0004 0 0 0.0004 0 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0147 0.0066 0.0062 0.0043 0.004 0.0176 0.0128 0.0038 0.0049 0.0037 0.0028 0.0053 0.0099 0.0025 0.0027 0.0033 0.0026 0.0027
Endosome 0.037 0.0133 0.0216 0.023 0.0081 0.032 0.082 0.019 0.0293 0.0841 0.0697 0.0345 0.0325 0.0158 0.0227 0.0458 0.0403 0.0305
Golgi 0.0042 0.001 0.0039 0.0016 0.0005 0.0105 0.015 0.0027 0.0064 0.0196 0.009 0.006 0.0081 0.0016 0.004 0.0158 0.0142 0.0024
Lipid Particles 0.016 0.0005 0.002 0.0007 0.0057 0.0019 0.0101 0.0003 0.002 0.0122 0.0011 0.0004 0.0107 0.0002 0.002 0.0112 0.0216 0.0008
Mitochondria 0.0028 0.0008 0.0026 0.0041 0.0338 0.0349 0.0187 0.0015 0.0066 0.0497 0.0008 0.0101 0.0049 0.0014 0.009 0.0047 0.0259 0.0034
None 0.2659 0.2266 0.258 0.0232 0.2429 0.1269 0.0659 0.0402 0.0269 0.0059 0.0044 0.0154 0.1263 0.0565 0.0659 0.1623 0.0612 0.0702
Nuclear Periphery 0.0348 0.001 0.0026 0.0053 0.001 0.0015 0.0192 0.0007 0.0021 0.0009 0.0004 0.0025 0.0041 0.0009 0.0053 0.0025 0.0016 0.0028
Nucleolus 0.002 0.0001 0.0011 0.0074 0.0604 0.0001 0.0031 0.0001 0.0003 0.0006 0.0003 0.0002 0.0006 0.0001 0.0012 0.0007 0.0003 0.0039
Nucleus 0.0223 0.001 0.0029 0.0391 0.0092 0.0009 0.0079 0.0016 0.0017 0.0012 0.0007 0.0033 0.0076 0.0015 0.0917 0.004 0.0019 0.0109
Peroxisomes 0.0046 0.0001 0.0024 0.0017 0.0017 0.0006 0.0035 0.0004 0.0032 0.0106 0.001 0.0015 0.0094 0.0005 0.003 0.0497 0.007 0.0013
Punctate Nuclear 0.0115 0.0003 0.0129 0.0025 0.0005 0.0008 0.0054 0.0008 0.003 0.0005 0.0004 0.0006 0.0058 0.0004 0.0044 0.0048 0.0019 0.0015
Vacuole 0.0038 0.0064 0.0071 0.0124 0.0068 0.004 0.0136 0.005 0.0069 0.012 0.0041 0.0034 0.0073 0.0036 0.0044 0.0066 0.0053 0.0032
Vacuole Periphery 0.0015 0.0008 0.0009 0.0024 0.0069 0.0014 0.0043 0.0006 0.0011 0.0026 0.0009 0.001 0.0015 0.0005 0.001 0.0015 0.0015 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.4923 25.4645 25.5649 34.3986 38.834 39.3556 37.0115 36.6718 35.4835 42.7385
Translational Efficiency 0.7572 0.7703 0.7671 0.5038 0.5074 0.7792 0.6066 0.6194 0.6425 0.6265

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2294 1415 295 1822 1489 1393 134 67 3783 2808 429 1889

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 827.83 858.75 1240.88 1002.64 808.14 812.15 1059.63 976.83 820.08 835.63 1184.27 1001.72
Standard Deviation 97.52 126.13 162.62 131.86 103.39 113.37 123.86 134.58 100.34 122.21 173.30 132.04
Intensity Change Log 2 0.052904 0.583957 0.276397 0.007141 0.390883 0.273502 0.030479 0.491807 0.274968

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000081 0.000212 0.000838 0.000545 0.000159 0.000400 0.000208 0.000243 0.000112 0.000305 0.000641 0.000535
Bud Neck 0.004795 0.005766 0.003004 0.003051 0.004572 0.009770 0.010275 0.004264 0.004708 0.007752 0.005275 0.003094
Bud Site 0.011982 0.018503 0.011186 0.030973 0.018553 0.022234 0.010686 0.044703 0.014568 0.020354 0.011030 0.031460
Cell Periphery 0.000132 0.000076 0.000219 0.000050 0.000123 0.000134 0.000107 0.000152 0.000128 0.000105 0.000184 0.000054
Cytoplasm 0.689227* 0.673723* 0.633192* 0.790863* 0.670620* 0.554416* 0.513722* 0.669796* 0.681903* 0.614537* 0.595875* 0.786569*
Cytoplasmic Foci 0.148207 0.168081 0.044261 0.029328 0.201860* 0.215322* 0.048890 0.118375 0.169325 0.191517* 0.045707 0.032486
Eisosomes 0.000041 0.000029 0.000025 0.000009 0.000041 0.000073 0.000053 0.000023 0.000041 0.000051 0.000034 0.000009
Endoplasmic Reticulum 0.001759 0.000530 0.002926 0.000216 0.001298 0.000534 0.001502 0.000283 0.001577 0.000532 0.002482 0.000218
Endosome 0.011213 0.015351 0.005379 0.003348 0.013017 0.027147 0.009999 0.006818 0.011923 0.021203 0.006822 0.003471
Golgi 0.007440 0.005066 0.000477 0.002154 0.008068 0.008184 0.000153 0.000516 0.007687 0.006612 0.000375 0.002096
Lipid Particles 0.001856 0.001163 0.002331 0.000272 0.001579 0.003319 0.002061 0.000546 0.001747 0.002233 0.002247 0.000282
Mitochondria 0.002232 0.003378 0.000228 0.001968 0.002512 0.004286 0.000061 0.000061 0.002342 0.003828 0.000176 0.001900
Mitotic Spindle 0.000784 0.006684 0.019901 0.024307 0.001323 0.003867 0.003676 0.004223 0.000996 0.005286 0.014833 0.023595
None 0.003504 0.000826 0.004581 0.001761 0.001869 0.000789 0.000795 0.001938 0.002861 0.000807 0.003398 0.001767
Nuclear Periphery 0.000070 0.000107 0.000894 0.000121 0.000046 0.000152 0.000772 0.000100 0.000060 0.000129 0.000856 0.000120
Nuclear Periphery Foci 0.000215 0.000279 0.006979 0.000685 0.000284 0.000322 0.004594 0.002840 0.000242 0.000300 0.006234 0.000761
Nucleolus 0.000372 0.000377 0.000152 0.000126 0.000216 0.000368 0.000178 0.000083 0.000310 0.000373 0.000160 0.000125
Nucleus 0.019561 0.019424 0.135138* 0.030473 0.015237 0.026184 0.057850 0.012812 0.017859 0.022778 0.110997 0.029847
Peroxisomes 0.000932 0.002280 0.000360 0.000784 0.001254 0.004616 0.000314 0.001538 0.001059 0.003439 0.000346 0.000811
Vacuole 0.091034 0.074863 0.127659 0.077189 0.053327 0.114867 0.333821* 0.130377 0.076192 0.094708 0.192054 0.079075
Vacuole Periphery 0.004564 0.003281 0.000268 0.001778 0.004042 0.003017 0.000282 0.000307 0.004359 0.003150 0.000272 0.001726

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.25 -8.87 -3.70 -1.96 5.11 -3.08 -3.44 -1.89 0.55 -0.41 -4.77 -8.99 -3.40 -0.80 4.14
Bud Neck -1.46 0.74 5.72 4.96 4.09 -6.42 -0.26 0.99 4.06 0.60 -5.90 -0.09 5.80 9.10 1.95
Bud Site -4.41 -0.82 -3.67 -0.11 -2.93 -2.02 3.62 -1.50 -1.18 -2.28 -5.08 2.49 -2.62 0.52 -3.84
Cell Periphery 6.31 -5.96 13.57 4.76 9.90 -0.74 0.14 -0.28 0.12 -0.33 2.35 -5.63 14.60 6.82 10.60
Cytoplasm 2.25 10.27 -4.13 -5.57 -11.96 12.12 9.03 2.61 -0.57 -2.92 10.88 13.39 -4.80 -13.32 -15.09
Cytoplasmic Foci -3.51 24.66 35.13 29.26 2.95 -1.93 27.49 6.82 7.62 -3.72 -5.01 36.02 45.74 43.03 2.71
Eisosomes 3.61 1.95 12.56 8.03 8.37 -7.21 -2.98 2.90 8.03 4.57 -3.45 -0.09 17.25 17.27 11.73
Endoplasmic Reticulum 4.97 -2.42 6.65 4.28 5.11 3.21 -1.76 3.98 2.47 6.79 5.95 -2.83 8.13 6.78 6.22
Endosome -3.44 6.54 15.45 12.33 6.31 -8.46 4.76 5.28 11.90 1.30 -9.17 8.18 18.13 20.57 7.91
Golgi 3.90 23.15 14.27 6.05 -1.52 -0.04 21.09 19.66 9.56 -2.28 2.17 31.07 16.68 9.44 -1.81
Lipid Particles 3.03 -4.86 10.37 3.74 10.24 -2.47 -1.31 4.63 3.82 4.67 -1.32 -5.04 12.29 4.98 11.55
Mitochondria -1.36 5.59 2.46 2.91 -1.53 -1.60 4.10 4.11 4.63 0.22 -2.19 6.72 2.89 4.15 -1.69
Mitotic Spindle -3.67 -3.67 -6.62 -3.06 0.91 -2.23 -1.09 -1.74 -0.85 0.12 -4.36 -3.73 -6.61 -4.01 0.37
None 5.20 0.36 3.73 -5.02 2.76 1.79 1.56 0.47 -0.67 -0.53 5.23 0.62 3.31 -5.06 2.21
Nuclear Periphery -1.77 -12.58 -11.03 -4.56 11.09 -2.59 -7.06 -3.61 -1.32 5.27 -3.02 -14.37 -12.76 -3.75 12.34
Nuclear Periphery Foci -1.89 -12.30 -9.32 -7.99 11.41 -0.79 -7.31 -3.35 -3.31 1.16 -1.94 -14.14 -9.29 -8.10 12.75
Nucleolus -0.10 3.35 4.10 1.62 2.17 -2.72 2.31 4.68 5.32 3.45 -0.93 3.84 4.58 3.04 1.75
Nucleus -0.22 -13.24 -15.20 -13.23 10.64 -5.31 -8.10 -0.17 3.28 7.48 -4.22 -14.52 -16.61 -11.32 10.87
Peroxisomes -6.14 11.28 2.95 6.92 -1.04 -6.52 13.40 0.27 4.81 -1.55 -8.50 17.02 3.83 9.43 -1.44
Vacuole 2.84 -9.37 -12.11 -14.34 4.01 -12.86 -14.53 -5.63 -3.83 3.50 -6.40 -15.81 -16.59 -11.93 8.39
Vacuole Periphery 3.36 17.76 11.61 6.17 -2.41 2.23 9.29 9.12 11.50 -0.63 4.26 18.75 11.81 7.99 -2.53
Standard name
Human Ortholog
Description Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Env10p/Snd2p and Pho88p/Snd3p; can compensate for loss of SRP; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Snd1

Snd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available