Standard name | |
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Human Ortholog | |
Description | Mitochondrial inner membrane protein; required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 2 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 4 | 3 | 0 | 0 | 0 | 5 | 8 | 16 |
Bud | 0 | 2 | – | 5 | 2 | 5 | 3 | – | 3 | 11 | 7 | 8 | 2 | 6 | 3 | 2 | 5 | 2 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 4 |
Bud Site | 0 | 0 | – | 0 | 0 | 4 | 0 | – | 1 | 2 | 7 | 5 | 1 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 3 | – | 3 | 5 | 0 | 1 | – | 0 | 1 | 5 | 3 | 12 | 17 | 7 | 16 | 9 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 1 | 0 | – | 4 | 8 | 2 | 6 | – | 5 | 4 | 4 | 1 | 11 | 18 | 10 | 10 | 9 | 12 | 0 | 0 | 0 | 1 | 2 | 1 |
Endoplasmic Reticulum | 2 | 4 | – | 3 | 2 | 1 | 0 | – | 2 | 2 | 0 | 3 | 8 | 0 | 1 | 9 | 2 | 4 | 0 | 0 | 0 | 0 | 2 | 2 |
Endosome | 17 | 0 | – | 9 | 21 | 0 | 17 | – | 2 | 7 | 2 | 4 | 6 | 34 | 28 | 4 | 3 | 3 | 12 | 3 | 0 | 18 | 29 | 24 |
Golgi | 5 | 3 | – | 15 | 43 | 0 | 8 | – | 0 | 4 | 2 | 3 | 48 | 51 | 67 | 58 | 64 | 19 | 8 | 7 | 2 | 16 | 33 | 38 |
Mitochondria | 187 | 94 | – | 166 | 176 | 183 | 191 | – | 235 | 270 | 306 | 382 | 257 | 402 | 345 | 11 | 9 | 3 | 210 | 77 | 42 | 262 | 335 | 293 |
Nucleus | 2 | 2 | – | 0 | 1 | 6 | 9 | – | 7 | 6 | 9 | 8 | 3 | 1 | 2 | 4 | 2 | 2 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 19 | 0 | – | 1 | 2 | 6 | 15 | – | 16 | 15 | 18 | 23 | 5 | 7 | 0 | 4 | 5 | 5 | 3 | 0 | 1 | 1 | 6 | 3 |
Nucleolus | 9 | 4 | – | 0 | 4 | 11 | 18 | – | 23 | 18 | 18 | 25 | 9 | 10 | 5 | 13 | 9 | 7 | 0 | 0 | 1 | 1 | 3 | 1 |
Peroxisomes | 5 | 0 | – | 0 | 4 | 0 | 1 | – | 1 | 2 | 0 | 3 | 6 | 29 | 21 | 6 | 1 | 3 | 5 | 1 | 2 | 15 | 22 | 25 |
SpindlePole | 5 | 0 | – | 3 | 6 | 4 | 7 | – | 3 | 2 | 3 | 5 | 2 | 3 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 |
Vac/Vac Membrane | 56 | 16 | – | 23 | 54 | 23 | 59 | – | 26 | 32 | 30 | 37 | 52 | 94 | 88 | 44 | 32 | 31 | 7 | 11 | 1 | 15 | 12 | 22 |
Unique Cell Count | 233 | 101 | 185 | 247 | 199 | 267 | 264 | 310 | 338 | 418 | 311 | 496 | 449 | 160 | 132 | 88 | 254 | 103 | 55 | 348 | 467 | 443 | ||
Labelled Cell Count | 312 | 128 | 234 | 328 | 246 | 335 | 325 | 376 | 411 | 510 | 423 | 673 | 579 | 187 | 154 | 98 | 254 | 103 | 55 | 348 | 467 | 443 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 5.9 | – | 6.4 | 7.7 | 5.0 | 7.4 | – | 6.3 | 6.8 | 6.5 | 6.8 | 7.5 | 8.1 | 8.3 | 13.1 | 13.5 | 13.2 | 6.3 | 6.5 | 6.7 |
Std Deviation (1e-4) | 1.0 | 1.0 | – | 1.1 | 1.6 | 1.3 | 1.8 | – | 1.4 | 1.6 | 1.7 | 1.6 | 1.2 | 1.6 | 1.5 | 3.2 | 2.7 | 3.4 | 1.3 | 1.8 | 1.4 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.4361 | 3.1477 | 2.9012 | 2.629 | 2.4667 | 3.0082 | 4.5562 | 4.9726 | 4.6665 | 4.0962 | 4.2448 | 4.9757 | 6.961 | 6.8792 | 6.5909 | 6.5053 | 6.2101 | 6.6887 |
Actin | 0.0052 | 0.001 | 0.0013 | 0.0009 | 0.0004 | 0.0003 | 0.0051 | 0.0034 | 0.0068 | 0.0003 | 0.0097 | 0.0095 | 0.0127 | 0.0058 | 0.01 | 0.0006 | 0.0004 | 0.0017 |
Bud | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0023 | 0 | 0.0002 | 0.0008 | 0.0005 | 0.0001 | 0.0023 | 0.0002 | 0.0011 | 0.0005 | 0.0002 | 0.0007 | 0.0006 | 0.0001 |
Bud Neck | 0.0009 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0007 |
Bud Periphery | 0.0013 | 0.0011 | 0.0007 | 0.0003 | 0.015 | 0.0001 | 0.0005 | 0.0014 | 0.001 | 0.0004 | 0.0063 | 0.0005 | 0.006 | 0.001 | 0.0007 | 0.0028 | 0.0015 | 0.0004 |
Bud Site | 0.0079 | 0.0004 | 0.0014 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0043 | 0.002 | 0.0001 | 0.0013 | 0.0003 | 0.0011 | 0.0023 | 0.0011 | 0.0003 | 0.0007 | 0.0001 |
Cell Periphery | 0.0007 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0007 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0041 | 0.0003 | 0.0005 | 0.0003 |
Cytoplasm | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.004 | 0.0003 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasmic Foci | 0.0063 | 0.0016 | 0.0047 | 0.0017 | 0.0046 | 0.0013 | 0.0126 | 0.0083 | 0.0029 | 0.0032 | 0.0186 | 0.0033 | 0.0042 | 0.0074 | 0.0037 | 0.0012 | 0.0039 | 0.001 |
Eisosomes | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0004 | 0.0002 | 0.0018 | 0 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0005 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0005 |
Endosome | 0.0223 | 0.0143 | 0.0106 | 0.0073 | 0.0146 | 0.0117 | 0.0309 | 0.025 | 0.0117 | 0.0132 | 0.0245 | 0.0158 | 0.0314 | 0.0389 | 0.022 | 0.0103 | 0.0458 | 0.0077 |
Golgi | 0.0236 | 0.0116 | 0.014 | 0.0116 | 0.0256 | 0.0118 | 0.0213 | 0.0247 | 0.0139 | 0.0205 | 0.0163 | 0.0208 | 0.0258 | 0.0486 | 0.0175 | 0.0036 | 0.027 | 0.0108 |
Lipid Particles | 0.0114 | 0.0035 | 0.0071 | 0.0017 | 0.0077 | 0.0028 | 0.0213 | 0.0056 | 0.0054 | 0.0085 | 0.0204 | 0.0105 | 0.0151 | 0.0131 | 0.0034 | 0.0009 | 0.0029 | 0.0074 |
Mitochondria | 0.8868 | 0.9332 | 0.9302 | 0.9708 | 0.9199 | 0.9616 | 0.8514 | 0.8907 | 0.9345 | 0.9394 | 0.8499 | 0.9101 | 0.8581 | 0.852 | 0.9018 | 0.9633 | 0.881 | 0.9536 |
None | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0104 | 0.0001 | 0.0004 | 0 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0005 | 0.0001 | 0.0015 | 0 | 0 | 0.0002 | 0.0042 | 0.0001 | 0.0011 | 0.0001 | 0.0014 | 0.0024 | 0.0019 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0042 |
Nucleolus | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0006 | 0.0007 | 0.0003 | 0.0002 | 0.0014 | 0.0002 | 0.0075 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0003 |
Nucleus | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0019 | 0.0003 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Peroxisomes | 0.0131 | 0.019 | 0.0069 | 0.0021 | 0.0037 | 0.0052 | 0.0082 | 0.017 | 0.0055 | 0.0077 | 0.0359 | 0.0071 | 0.0069 | 0.0091 | 0.005 | 0.0032 | 0.004 | 0.002 |
Punctate Nuclear | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 | 0.0051 | 0.0001 | 0.0001 | 0 | 0.0041 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Vacuole | 0.0087 | 0.0039 | 0.0073 | 0.0009 | 0.002 | 0.001 | 0.0074 | 0.0093 | 0.0047 | 0.0016 | 0.0018 | 0.0052 | 0.0117 | 0.0068 | 0.017 | 0.006 | 0.018 | 0.0022 |
Vacuole Periphery | 0.0079 | 0.0085 | 0.0106 | 0.0018 | 0.0028 | 0.0033 | 0.013 | 0.0064 | 0.0074 | 0.0041 | 0.0026 | 0.0123 | 0.014 | 0.0118 | 0.011 | 0.0059 | 0.0123 | 0.0064 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.3181 | 11.1725 | 35.5308 | 22.2163 | 10.4171 | 41.0425 | 25.0138 | 28.3192 | 39.2302 | 19.2694 |
Translational Efficiency | 0.993 | 2.4519 | 0.6604 | 1.8771 | 1.9894 | 0.6509 | 1.0422 | 1.2452 | 0.9605 | 0.9952 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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