Standard name | |
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Human Ortholog | |
Description | U1 snRNP protein involved in splicing; required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 1 | 7 | 5 | 12 | – | 2 | 6 | 12 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 2 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 15 | 15 | 11 | – | 32 | 25 | 24 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 8 | 3 | 16 | 29 | 21 | 31 | 44 | – | 27 | 19 | 28 | 10 | 10 | 6 | 12 | 15 | 8 | 1 | 0 | 0 | 0 | 2 | 0 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 3 | 2 | 1 | 2 | 6 | 3 | 0 | 0 | 0 | 2 | 0 | 3 |
Endosome | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | – | 1 | 0 | 0 | 1 | 3 | 2 | 3 | 4 | 0 | 2 | 2 | 4 | 3 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 2 | 2 |
Mitochondria | 10 | 9 | 73 | 89 | 106 | 277 | 422 | 557 | – | 341 | 391 | 438 | 8 | 6 | 13 | 7 | 17 | 3 | 2 | 2 | 1 | 0 | 2 | 0 |
Nucleus | 197 | 109 | 215 | 241 | 269 | 334 | 421 | 573 | – | 336 | 347 | 364 | 243 | 254 | 309 | 175 | 223 | 133 | 183 | 102 | 180 | 148 | 240 | 241 |
Nuclear Periphery | 0 | 0 | 2 | 3 | 0 | 3 | 2 | 2 | – | 5 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 20 | 16 | 5 | 2 | 3 | 8 | 16 | 56 | – | 24 | 27 | 23 | 1 | 4 | 1 | 2 | 4 | 3 | 18 | 9 | 31 | 11 | 24 | 31 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 3 | 0 | 0 | 6 | 7 | 10 | – | 9 | 1 | 6 | 10 | 6 | 6 | 4 | 2 | 3 | 0 | 1 | 2 | 3 | 2 | 7 |
Vac/Vac Membrane | 3 | 3 | 3 | 3 | 6 | 3 | 4 | 4 | – | 3 | 7 | 3 | 2 | 1 | 4 | 5 | 13 | 8 | 7 | 10 | 14 | 6 | 21 | 35 |
Unique Cell Count | 216 | 120 | 233 | 284 | 326 | 438 | 595 | 827 | 509 | 515 | 570 | 268 | 276 | 331 | 194 | 254 | 153 | 224 | 132 | 240 | 181 | 310 | 334 | |
Labelled Cell Count | 236 | 146 | 304 | 356 | 416 | 674 | 924 | 1271 | 780 | 825 | 900 | 282 | 286 | 343 | 212 | 285 | 162 | 224 | 132 | 240 | 181 | 310 | 334 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 6.2 | 4.3 | 4.3 | 4.2 | 3.7 | 3.2 | 3.4 | – | 3.4 | 3.0 | 3.1 | 6.1 | 6.0 | 5.9 | 6.7 | 6.6 | 6.9 | 7.1 | 7.4 | 7.7 |
Std Deviation (1e-4) | 1.4 | 1.2 | 0.8 | 1.3 | 1.5 | 0.9 | 1.0 | 1.2 | – | 5.5 | 0.9 | 0.8 | 1.1 | 1.2 | 0.9 | 1.2 | 1.5 | 1.3 | 1.7 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.04 | -0.21 | -0.42 | -0.33 | – | -0.35 | -0.5 | -0.5 | 0.5 | 0.48 | 0.45 | 0.64 | 0.61 | 0.68 | 0.73 | 0.77 | 0.84 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.6 | 3.8 | 2.3 | 2.6 | 2.6 | – | 2.6 | 1.8 | 2.4 | 1.7 | 1.7 | 0 | 2.7 | 2.7 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -8.6 | -9.1 | -8.9 | -7.3 | -7.0 | -7.1 |
Nucleus | -2.6 | -3.3 | -5.1 | -6.6 | -7.1 | – | -7.6 | -7.3 | -8.1 | -0.6 | -0.1 | 0.5 | -0.8 | -1.6 | -1.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0264 | 0 | 0.0124 | 0.0108 | 0.0158 | 0.0002 | 0.0071 | 0 | 0.0042 | 0 | 0 | 0.0038 | 0.0565 | 0 | 0.0002 | 0 | 0.0035 | 0.0003 |
Bud | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.001 | 0.0001 | 0.0005 | 0 | 0.0009 | 0.0001 |
Bud Neck | 0.0011 | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0008 | 0.0008 | 0.0003 | 0.0007 | 0.0001 | 0.0012 | 0.0012 | 0.0011 | 0.0002 | 0.0004 | 0.0001 | 0.0081 | 0.0043 |
Bud Periphery | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0009 | 0.0001 | 0.0014 | 0 | 0.0006 | 0.0002 |
Bud Site | 0.0019 | 0.0003 | 0.0026 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0 | 0.0001 | 0 | 0.0067 | 0.0003 | 0.0003 | 0.0001 | 0.0069 | 0.0007 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.0033 | 0.0009 | 0.0008 | 0.0002 | 0.0012 | 0.0004 | 0.0008 | 0.0003 | 0.0004 | 0.0007 | 0.0005 | 0.0009 | 0.1174 | 0.0001 | 0.0014 | 0.0002 | 0.0276 | 0.0271 |
Cytoplasmic Foci | 0.0141 | 0 | 0.0021 | 0.0037 | 0.0008 | 0.0002 | 0.001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0315 | 0 | 0 | 0 | 0.1297 | 0.0003 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0014 | 0.0001 | 0.0004 | 0.0002 | 0.0025 | 0.0002 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0186 | 0 | 0.0003 | 0 | 0.0017 | 0.0004 |
Endosome | 0.0136 | 0.0006 | 0.0019 | 0.004 | 0.0093 | 0.0008 | 0.0013 | 0 | 0.0009 | 0 | 0.0001 | 0.0002 | 0.0242 | 0 | 0.0001 | 0 | 0.1413 | 0.0007 |
Golgi | 0.0054 | 0 | 0.0016 | 0.003 | 0.0027 | 0 | 0.0009 | 0 | 0.0004 | 0 | 0 | 0.0002 | 0.0319 | 0 | 0 | 0 | 0.0343 | 0 |
Lipid Particles | 0.0119 | 0 | 0.0007 | 0.0211 | 0.0005 | 0.0001 | 0.0018 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0.0156 | 0 | 0 | 0 | 0.0043 | 0.0001 |
Mitochondria | 0.0021 | 0.0004 | 0.0009 | 0.0034 | 0.0013 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0112 | 0.0004 | 0.0017 | 0.0005 | 0.0047 | 0.0004 |
None | 0.0038 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0025 | 0.0003 | 0.0064 | 0.0012 | 0.0005 | 0.0028 | 0.0127 | 0 | 0.0009 | 0.0001 | 0.0607 | 0.0109 |
Nuclear Periphery | 0.0111 | 0.0048 | 0.0045 | 0.0012 | 0.0067 | 0.0036 | 0.0073 | 0.0012 | 0.006 | 0.0018 | 0.0032 | 0.0019 | 0.0123 | 0.0005 | 0.0057 | 0.0012 | 0.0077 | 0.0052 |
Nucleolus | 0.0239 | 0.0167 | 0.0156 | 0.0141 | 0.0285 | 0.0422 | 0.0152 | 0.0156 | 0.0088 | 0.011 | 0.0361 | 0.0452 | 0.013 | 0.0164 | 0.0171 | 0.0095 | 0.0671 | 0.0717 |
Nucleus | 0.8505 | 0.9689 | 0.936 | 0.918 | 0.9262 | 0.9369 | 0.9518 | 0.9548 | 0.9602 | 0.9681 | 0.94 | 0.9315 | 0.6126 | 0.9812 | 0.9657 | 0.9878 | 0.3555 | 0.859 |
Peroxisomes | 0.0154 | 0 | 0.0035 | 0.0149 | 0.0003 | 0.0001 | 0.0014 | 0 | 0.0001 | 0 | 0 | 0 | 0.0226 | 0 | 0 | 0 | 0.0047 | 0 |
Punctate Nuclear | 0.0108 | 0.004 | 0.0153 | 0.0035 | 0.0022 | 0.0135 | 0.0066 | 0.0272 | 0.0088 | 0.0169 | 0.0178 | 0.0117 | 0.0039 | 0.0003 | 0.0015 | 0.0004 | 0.1348 | 0.0176 |
Vacuole | 0.0016 | 0.0021 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0047 | 0.0001 | 0.0015 | 0.0001 | 0.0041 | 0.0006 |
Vacuole Periphery | 0.0008 | 0.0005 | 0.0002 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0011 | 0.0001 | 0.0011 | 0 | 0.0013 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.1353 | 13.2723 | 7.6103 | 11.2773 | 13.5624 | 8.0939 | 9.6072 | 13.1743 | 12.0346 | 14.8181 |
Translational Efficiency | 0.7417 | 0.5782 | 0.7215 | 0.5635 | 0.5457 | 1.0571 | 1.0721 | 0.4903 | 0.5418 | 0.5515 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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