ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.07
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0.09 0.06 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0 0.06 0.09 0 0 0 0 0 – 0 0 0.08 0.07 0.07 0.11 0.19 0.12 0 0 0 0 0 0
Cytoplasm 0.88 0.94 0.98 0.99 0.96 0.96 0.92 0.93 0.81 – 0.82 0.84 0.99 0.98 0.99 0.94 0.95 0.92 0.75 0.83 0.86 0.59 0.61 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.14 0.09 0.31 – 0.37 0.27 0 0 0 0 0 0 0 0 0 0.08 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 1 1 0 0 0 0 2 0 – 3 1 0 2 1 9 3 8 3 4 3 8 27 28
Bud 4 4 4 2 2 1 1 5 7 – 5 1 4 2 2 3 2 1 2 2 1 9 20 16
Bud Neck 0 0 2 0 0 0 2 2 0 – 0 0 0 0 0 0 0 0 7 6 2 14 23 12
Bud Site 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 7 10 14 11 7 12 5 5 – 5 3 28 30 30 86 88 61 3 1 2 2 14 12
Cytoplasm 89 156 172 147 272 198 307 276 198 – 114 160 349 400 428 704 447 473 83 147 152 94 226 268
Endoplasmic Reticulum 3 3 0 0 4 0 2 0 0 – 0 3 2 2 1 27 10 20 2 0 0 2 6 8
Endosome 0 0 0 0 0 1 0 1 0 – 0 0 1 2 1 2 0 1 0 2 1 1 5 3
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 1 0 0 1 0 0 2 0
Mitochondria 2 6 1 1 2 20 47 28 75 – 51 52 3 1 1 7 2 2 0 3 7 12 18 14
Nucleus 1 0 0 0 1 1 0 3 1 – 1 0 0 0 1 12 8 10 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 2 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 2 1 1 2 0 – 0 2 0 3 0 3 2 0 0 1 1 0 2 3
Vac/Vac Membrane 0 0 0 0 1 2 3 3 4 – 1 3 1 0 0 28 6 20 3 5 3 10 17 14
Unique Cell Count 101 166 175 149 282 207 332 296 245 139 191 353 407 434 752 469 516 112 178 176 160 372 389
Labelled Cell Count 110 178 191 164 295 231 375 327 292 180 226 389 443 466 883 570 596 112 178 176 160 372 389


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.3 5.8 5.5 6.3 4.8 5.2 5.4 4.3 – 4.0 4.5 5.7 5.9 6.0 10.2 10.5 10.4 5.3 5.3 5.8
Std Deviation (1e-4) 0.5 1.0 0.8 1.0 1.4 0.9 1.2 1.4 0.9 – 1.0 1.2 1.0 1.0 1.1 1.9 2.2 1.9 1.2 1.1 1.4
Intensity Change (Log2) – – – -0.08 0.11 -0.28 -0.16 -0.11 -0.45 – -0.55 -0.36 -0.04 0.01 0.04 0.8 0.85 0.83 -0.13 -0.14 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 1.3 -0.9 0 -1.1 -2.4 0 – 0 0 0.9 0.7 0.5 2.2 4.1 2.3
Cytoplasm 0.3 -1.1 -1.5 -2.7 -2.5 -5.5 – -5.0 -4.8 0.5 0 0.3 -2.4 -1.7 -3.0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 2.5 0 2.6
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 5.0 3.9 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 2.6 0 2.6