Standard name
Human Ortholog
Description Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0.07 0.07 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.39 0.53 0.45 0.39 0.25 0.31 0.25 0.18 0.21 0.18 0.16 0.31 0.38 0.41 0.25 0.25 0.18 0.25 0.38 0.26 0.23 0.28
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0 0 0 0 0 0
Endosome 0.08 0.08 0 0 0.06 0 0 0 0 0 0 0 0.37 0.33 0.39 0.51 0.42 0.17 0.18 0.06 0.1 0.12 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.45 0.55 0.59 0.34 0.72 0.61 0.59 0.73 0.77 0.8 0.82 0.07 0.06 0 0.07 0.09 0.07 0.08 0.11 0.1 0.1 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.09 0 0.05 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
SpindlePole 0.18 0.08 0.13 0.08 0.28 0.17 0.29 0.3 0.24 0.18 0.18 0.2 0.13 0.12 0.1 0.19 0.12 0.39 0.28 0.24 0.38 0.38 0.31
Vac/Vac Membrane 0.1 0.29 0.06 0 0 0 0 0 0 0 0 0 0.3 0.25 0.24 0.25 0.34 0.08 0.07 0.12 0.05 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 1 5 10 19
Bud 10 1 1 4 3 11 18 25 16 15 8 19 0 3 2 2 0 1 0 1 5 14 26
Bud Neck 1 0 0 0 5 9 6 18 5 6 5 2 1 1 0 0 0 1 0 0 3 3 19
Bud Site 8 0 0 1 2 8 12 11 8 12 5 8 1 1 2 0 1
Cell Periphery 1 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 2
Cytoplasm 97 44 28 51 62 55 83 85 47 61 41 48 149 234 353 37 30 78 37 44 95 165 211
Endoplasmic Reticulum 0 0 0 0 0 2 0 1 1 0 1 2 1 1 1 14 8 0 0 0 2 4 7
Endosome 26 9 0 1 10 2 2 1 0 0 1 1 180 204 333 77 50 75 25 7 35 87 65
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 2 2 0 1 3 1
Mitochondria 159 50 29 67 55 160 166 203 187 228 184 248 32 36 17 10 11 31 12 12 38 73 83
Nucleus 4 2 0 2 2 5 6 7 7 5 7 6 24 27 10 5 11 11 7 1 7 11 7
Nuclear Periphery 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0
Nucleolus 12 1 0 2 1 2 7 3 5 2 3 2 7 4 9 0 2 3 0 0 3 5 2
Peroxisomes 3 0 0 0 0 2 2 2 1 1 3 0 3 5 15 13 3 0 0 0 1 1 1
SpindlePole 57 9 7 9 45 39 78 105 61 53 42 60 61 75 88 29 15 167 40 27 139 265 232
Vac/Vac Membrane 33 33 3 0 3 4 2 1 1 3 1 2 144 155 211 37 41 33 10 13 18 30 49
Unique Cell Count 319 112 53 113 161 223 272 346 256 295 229 304 487 610 864 150 120 431 146 116 370 707 754
Labelled Cell Count 411 150 68 138 189 299 382 462 339 386 301 398 605 747 1042 225 172 431 146 116 370 707 754


Microtubule

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.6 3.4 3.1 3.5 2.9 2.8 2.9 2.6 2.3 2.6 2.3 4.6 4.3 4.3 4.3 5.2 5.9 4.2 4.1 4.6
Std Deviation (1e-4) 0.6 0.7 1.5 0.6 1.1 0.9 1.5 0.9 0.9 0.5 0.9 0.5 1.3 0.9 1.1 1.2 1.1 1.6 1.4 1.2 1.6
Intensity Change (Log2) -0.16 0.01 -0.25 -0.29 -0.22 -0.38 -0.56 -0.41 -0.6 0.41 0.31 0.31 0.33 0.58 0.78 0.28 0.25 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 3.0 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4853 0.9708 0.9602 0.7177 1.0909 0.8552 0.1358 -0.3547 -0.1517 -0.5159 -0.7977 -0.2033 0.8181 1.3817 1.056 1.1576 0.9865 1.0028
Actin 0.0311 0.0018 0.0159 0.0007 0.0281 0.0078 0.0412 0.0004 0.0105 0.0025 0.0003 0.0031 0.0079 0.0008 0.0061 0.0126 0.001 0.0025
Bud 0.0007 0.005 0.0053 0.0001 0.0003 0.0021 0.0018 0.0001 0.0002 0 0 0.0001 0.0003 0.0064 0.001 0.0003 0.0001 0.0002
Bud Neck 0.0048 0.0017 0.0036 0.001 0.0083 0.0103 0.0118 0.0004 0.0006 0.0004 0.0006 0.0024 0.0034 0.0007 0.0013 0.044 0.0031 0.0064
Bud Periphery 0.0014 0.0049 0.0013 0 0.0003 0.0005 0.0036 0.0001 0.0004 0 0 0.0001 0.0003 0.0014 0.0006 0.0004 0.0001 0.0004
Bud Site 0.0045 0.0102 0.0135 0.0002 0.0012 0.0005 0.0055 0.0012 0.0008 0.0002 0.0002 0.0002 0.0052 0.0153 0.0016 0.0038 0.0005 0.0006
Cell Periphery 0.0004 0.0002 0.0003 0 0.0001 0.0001 0.0004 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0001 0.0003 0 0.0001
Cytoplasm 0.1004 0.2144 0.1361 0.1732 0.2007 0.1522 0.0689 0.2914 0.0701 0.0429 0.016 0.1445 0.1843 0.1354 0.1341 0.1337 0.0907 0.1292
Cytoplasmic Foci 0.1062 0.0552 0.0506 0.0526 0.1097 0.0503 0.0471 0.013 0.029 0.06 0.0414 0.0353 0.0969 0.0407 0.038 0.0713 0.17 0.046
Eisosomes 0.0004 0.0001 0.0002 0 0.0002 0.0001 0.0003 0.0001 0.0004 0.0001 0.0002 0.0003 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0106 0.0098 0.0029 0.0058 0.0088 0.0045 0.0062 0.008 0.0025 0.0045 0.002 0.0098 0.0047 0.0044 0.0054 0.0044 0.0027 0.0028
Endosome 0.0506 0.021 0.0109 0.0121 0.0959 0.014 0.0403 0.0174 0.0075 0.0135 0.0098 0.0128 0.0211 0.011 0.0247 0.0252 0.0351 0.011
Golgi 0.0106 0.0007 0.0055 0.0006 0.0053 0.0011 0.0108 0.0005 0.0027 0.0011 0.0012 0.0012 0.007 0.0007 0.0053 0.0109 0.0018 0.0013
Lipid Particles 0.0347 0.004 0.0048 0.0016 0.0132 0.0036 0.0352 0.0043 0.0142 0.0061 0.0226 0.0051 0.0205 0.0072 0.0113 0.0078 0.0168 0.0103
Mitochondria 0.0089 0.0008 0.0015 0.0006 0.0017 0.0014 0.0069 0.0007 0.0096 0.0009 0.0004 0.0011 0.0022 0.0039 0.0044 0.0047 0.0006 0.001
None 0.2793 0.3465 0.2338 0.2422 0.2097 0.2084 0.486 0.4307 0.6489 0.5446 0.4758 0.5014 0.2948 0.3753 0.3574 0.3164 0.3167 0.3018
Nuclear Periphery 0.0306 0.0165 0.0225 0.0124 0.0159 0.021 0.0472 0.0167 0.0112 0.0532 0.0061 0.0211 0.0165 0.0235 0.0318 0.0277 0.0092 0.017
Nucleolus 0.0107 0.0045 0.0072 0.002 0.0069 0.0073 0.0042 0.0035 0.0025 0.0019 0.0057 0.0029 0.0043 0.0044 0.0054 0.0026 0.0049 0.0049
Nucleus 0.1146 0.1475 0.1883 0.2063 0.0575 0.1746 0.081 0.1695 0.0508 0.0143 0.0133 0.1017 0.0729 0.1659 0.1325 0.0746 0.0695 0.1428
Peroxisomes 0.0186 0.0014 0.0091 0.0131 0.0103 0.0094 0.0078 0.0005 0.013 0.0028 0.0047 0.0048 0.0115 0.006 0.0074 0.0061 0.0108 0.0051
Punctate Nuclear 0.1741 0.1476 0.2849 0.2745 0.2197 0.3285 0.0835 0.0367 0.1224 0.2493 0.3983 0.1504 0.2435 0.1943 0.2283 0.2504 0.2639 0.3151
Vacuole 0.0046 0.0052 0.0014 0.0007 0.0053 0.0018 0.0056 0.004 0.0014 0.0006 0.0012 0.0013 0.0021 0.002 0.0022 0.0019 0.0022 0.0011
Vacuole Periphery 0.0021 0.0008 0.0004 0.0002 0.0009 0.0005 0.0044 0.0006 0.0012 0.0011 0.0002 0.0005 0.0004 0.0005 0.0008 0.0007 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2118 9.3122 5.847 6.7671 9.3504 6.4069 10.683 9.5323 9.1861 10.1818
Translational Efficiency 1.0408 0.7416 0.7979 0.9184 0.6855 0.97 0.731 0.6522 0.6925 0.8054

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1254 612 1672 1091 1301 909 1940 793 2555 1521 3612 1884

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 603.16 644.61 769.86 792.29 644.32 671.33 813.33 882.72 624.12 660.58 793.21 830.35
Standard Deviation 70.50 143.75 87.10 118.77 91.32 87.21 86.95 127.89 84.31 114.16 89.68 130.56
Intensity Change Log 2 0.095886 0.352055 0.393488 0.059245 0.336063 0.454179 0.077077 0.343818 0.425153

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000077 0.000627 0.001344 0.003431 0.000147 0.000403 0.000289 0.003037 0.000112 0.000493 0.000777 0.003265
Bud Neck 0.004313 0.019337 0.007453 0.014568 0.003984 0.020571 0.009319 0.015619 0.004146 0.020075 0.008455 0.015010
Bud Site 0.003083 0.006440 0.005759 0.051426 0.001520 0.005804 0.012536 0.081465 0.002287 0.006060 0.009399 0.064069
Cell Periphery 0.000138 0.000258 0.000163 0.000223 0.000157 0.000149 0.000179 0.001275 0.000148 0.000192 0.000172 0.000666
Cytoplasm 0.420902* 0.250755* 0.144041* 0.262480* 0.413776* 0.265018* 0.226555* 0.237546* 0.417273* 0.259279* 0.188359* 0.251985*
Cytoplasmic Foci 0.149106 0.263467* 0.006560 0.009284 0.099257 0.313735* 0.010186 0.039446 0.123723 0.293509* 0.008508 0.021979
Eisosomes 0.000069 0.000240 0.000056 0.000058 0.000069 0.000149 0.000040 0.000055 0.000069 0.000186 0.000047 0.000057
Endoplasmic Reticulum 0.000478 0.001799 0.005098 0.005912 0.000931 0.001082 0.002637 0.004100 0.000709 0.001371 0.003776 0.005149
Endosome 0.003418 0.035790 0.002983 0.007772 0.004585 0.018020 0.002181 0.015242 0.004012 0.025170 0.002552 0.010916
Golgi 0.000984 0.008279 0.000373 0.008848 0.000848 0.004933 0.000277 0.007340 0.000915 0.006279 0.000321 0.008213
Lipid Particles 0.007551 0.034072 0.002151 0.001537 0.007809 0.034079 0.001227 0.004222 0.007682 0.034076 0.001655 0.002667
Mitochondria 0.002348 0.022504 0.002250 0.003193 0.003786 0.018038 0.001396 0.015958 0.003080 0.019835 0.001792 0.008566
Mitotic Spindle 0.006499 0.035329 0.034463 0.102686* 0.006137 0.035117 0.031311 0.122516* 0.006314 0.035202 0.032770 0.111033*
None 0.053295 0.045782 0.012001 0.015535 0.043241 0.008961 0.012742 0.014029 0.048176 0.023777 0.012399 0.014901
Nuclear Periphery 0.000682 0.002163 0.004160 0.002475 0.000896 0.001597 0.002554 0.006541 0.000791 0.001825 0.003298 0.004186
Nuclear Periphery Foci 0.002288 0.005864 0.004832 0.004682 0.002923 0.003071 0.002013 0.005675 0.002612 0.004195 0.003318 0.005100
Nucleolus 0.022916 0.029121 0.012310 0.004201 0.020712 0.014648 0.008824 0.003367 0.021794 0.020472 0.010437 0.003850
Nucleus 0.315004* 0.173825* 0.736253* 0.452030* 0.378815* 0.189927* 0.659845* 0.350641* 0.347496* 0.183448* 0.695214* 0.409354*
Peroxisomes 0.003490 0.027233 0.000927 0.003972 0.003067 0.036813 0.000460 0.005362 0.003274 0.032958 0.000676 0.004557
Vacuole 0.002972 0.033429 0.016403 0.042331 0.006870 0.026415 0.015142 0.059012 0.004957 0.029237 0.015726 0.049352
Vacuole Periphery 0.000388 0.003687 0.000421 0.003358 0.000472 0.001469 0.000286 0.007553 0.000430 0.002361 0.000348 0.005124

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.16 -6.13 -7.49 -5.27 -2.69 -4.52 -9.95 -5.75 -5.11 -4.01 -5.93 -8.89 -9.40 -7.63 -4.94
Bud Neck -6.11 -13.70 -11.86 -1.93 -2.16 -7.33 -15.35 -11.38 -1.29 -0.34 -9.51 -20.55 -16.33 -2.33 -1.79
Bud Site -3.22 -5.64 -8.82 -7.64 -6.61 -5.02 -6.61 -9.61 -8.63 -7.26 -5.45 -8.56 -13.10 -11.56 -9.68
Cell Periphery -3.73 -3.34 -6.84 0.93 -3.71 2.32 1.08 -7.77 -8.16 -7.94 -2.03 -1.30 -8.90 -7.36 -8.52
Cytoplasm 8.07 29.36 19.65 7.20 -7.61 11.17 25.04 21.19 8.48 -0.45 13.63 37.91 28.92 11.28 -5.01
Cytoplasmic Foci -5.55 28.95 29.17 26.23 1.09 -21.02 23.86 21.35 36.87 -3.05 -18.39 36.89 35.98 45.44 -1.68
Eisosomes -3.57 -5.43 -3.24 3.07 1.43 -8.65 2.02 -4.59 2.80 -6.05 -5.68 -2.25 -5.37 4.03 -3.50
Endoplasmic Reticulum -7.21 -23.48 -21.51 -17.72 -2.54 -0.41 -19.86 -17.33 -17.10 -6.57 -6.23 -30.57 -27.50 -25.15 -6.85
Endosome -11.68 -1.23 -1.40 11.28 -0.48 -9.50 4.67 -1.08 7.34 -3.97 -15.05 2.64 -1.46 13.90 -3.74
Golgi -5.64 6.30 -0.89 3.72 -2.12 -4.45 9.29 -0.76 2.89 -2.36 -7.07 10.30 -1.17 4.30 -2.96
Lipid Particles -7.68 10.32 11.16 10.11 2.01 -9.08 15.35 9.39 11.40 -3.93 -11.88 17.95 15.04 15.32 -2.28
Mitochondria -5.81 1.56 1.83 5.97 0.33 -6.46 3.99 -1.03 4.65 -2.50 -8.73 4.33 -0.39 8.17 -2.43
Mitotic Spindle -5.09 -9.99 -13.95 -8.55 -7.30 -5.78 -9.94 -11.39 -7.14 -5.94 -7.77 -14.12 -17.97 -11.27 -9.52
None 3.81 17.64 16.99 4.64 -1.29 16.07 14.08 14.77 -1.85 2.51 11.52 22.45 22.41 3.68 0.93
Nuclear Periphery -4.48 -20.69 -14.53 -8.25 1.98 -3.45 -20.61 -8.39 -7.48 -1.73 -5.78 -29.08 -15.24 -11.81 -0.41
Nuclear Periphery Foci -2.69 -7.84 -6.15 -2.77 -0.13 1.33 -4.11 -3.84 -4.48 -1.33 -1.56 -8.64 -7.25 -5.65 -1.37
Nucleolus 4.66 0.87 11.60 4.89 11.39 5.53 0.60 8.16 2.27 8.11 7.55 1.15 14.12 5.12 13.90
Nucleus 8.42 -36.41 -18.54 -24.27 11.88 15.82 -27.35 -7.59 -20.19 13.51 16.98 -44.75 -18.45 -32.11 17.45
Peroxisomes -7.96 11.74 8.42 10.20 -0.72 -14.01 11.90 4.96 15.28 -1.76 -15.87 16.73 9.50 18.44 -1.94
Vacuole -11.33 -14.35 -17.38 -9.03 -10.48 -8.53 -11.34 -15.82 -12.95 -12.82 -14.15 -18.20 -23.04 -16.09 -16.58
Vacuole Periphery -5.34 -0.18 -1.65 3.38 -1.63 -3.80 5.33 -3.10 -1.35 -3.57 -6.51 2.47 -3.23 1.70 -3.60
Standard name
Human Ortholog
Description Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15
Localization
Cell Percentages nucleus (21%), cytoplasm (11%), mixed (37%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Chl4

Chl4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Chl4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available