Standard name
Human Ortholog
Description RING finger ubiquitin ligase (E3); involved in ubiquitination and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.98 0.99 0.95 0.92 0.88 0.89 0.78 0.82 0.6 0.73 1.0 0.99 0.99 0.91 0.94 0.89 0.96 0.93 0.92 0.94 0.94 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.13 0.1 0.19 0.15 0.44 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0.07 0 0.05 0 0 0 0.07 0.05 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 1 2 0 1 1 0 0 0 0 0 0 1 0 1 0 0 0 0
Bud 0 2 0 1 1 0 1 4 3 1 8 2 0 0 0 0 0 0 0 1 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 9 11 7 13 10 14 4 7 6 3 9 1 6 6 15 12 11 0 0 0 0 0 0
Cytoplasm 275 447 199 246 315 284 410 401 243 319 150 183 252 270 318 218 198 218 260 441 189 180 296 287
Endoplasmic Reticulum 0 9 3 0 1 4 8 5 9 6 7 6 2 3 0 15 6 6 0 3 3 1 2 0
Endosome 0 1 0 0 1 0 1 1 1 2 2 3 0 1 0 2 1 1 1 4 1 0 2 2
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 3 0 0 1 2
Mitochondria 3 1 0 3 13 20 59 44 60 60 109 60 0 0 0 6 5 4 1 6 2 0 2 1
Nucleus 0 1 2 0 0 2 3 5 2 5 5 4 0 1 1 2 0 5 0 2 0 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 1 1 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 3 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 2 1 4 10 11 11 15 15 27 8 12 0 1 1 16 10 28 0 3 3 1 0 0
Unique Cell Count 278 462 204 249 330 310 465 453 310 391 249 250 253 273 320 240 211 244 272 474 207 191 316 301
Labelled Cell Count 281 474 216 262 354 331 509 484 341 428 293 281 255 282 327 275 233 274 272 474 207 191 316 301


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 7.5 8.2 8.3 7.9 6.8 6.3 6.5 5.5 5.8 5.1 5.5 8.8 8.7 8.2 8.5 8.7 8.2 8.0 8.5 8.3
Std Deviation (1e-4) 0.8 1.0 1.1 1.4 1.4 1.4 1.1 1.5 1.1 1.2 1.1 1.3 1.0 1.1 1.2 1.5 1.6 1.6 1.4 1.3 1.3
Intensity Change (Log2) – – – 0.02 -0.05 -0.27 -0.37 -0.33 -0.58 -0.48 -0.67 -0.57 0.1 0.1 0.01 0.06 0.09 0.0 -0.03 0.05 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -0.8 -1.2 -1.5 0 -1.9 -2.7 -2.6 -0.9 -3.3 -1.9 -2.2 0.4 0.1 -0.4
Cytoplasm 1.0 -1.2 -2.8 -3.9 -3.8 -6.1 -5.5 -9.4 -7.1 1.9 1.1 1.8 -3.0 -1.9 -3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 5.3 4.6 6.7 5.9 10.8 7.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 2.8 3.5 0 2.7 0 0 0 3.4 2.7 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3908 4.9353 4.3477 4.3048 3.5552 4.4231 3.1407 5.194 4.0526 3.2019 3.1149 4.3689 3.845 5.3678 4.6153 3.9223 4.2959 4.8772
Actin 0.0415 0.0004 0.0144 0.0061 0.0135 0.0016 0.0098 0.0001 0.0224 0.0018 0.0136 0.0093 0.0773 0.0008 0.0198 0.0197 0.0082 0.0127
Bud 0.0008 0.0037 0.0004 0.0043 0.0004 0.0028 0.0004 0.0001 0.0015 0.0002 0.0112 0.0001 0.0005 0.0023 0.0012 0.0006 0.0005 0.0001
Bud Neck 0.0085 0.0001 0.0015 0.0009 0.0005 0.0009 0.0016 0.0001 0.0006 0.0003 0.0084 0.0015 0.0017 0.0002 0.0017 0.0092 0.003 0.0014
Bud Periphery 0.0008 0.001 0.0004 0.0021 0.0003 0.0017 0.0004 0 0.0062 0.0002 0.0116 0.0001 0.0005 0.0005 0.0004 0.001 0.001 0.0001
Bud Site 0.006 0.0024 0.002 0.0038 0.0007 0.0002 0.001 0.0007 0.0044 0.0003 0.0034 0.0002 0.0021 0.0026 0.0011 0.0109 0.0011 0.0001
Cell Periphery 0.0003 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0003 0.0002 0.0001 0.0014 0.0002 0
Cytoplasm 0.6963 0.959 0.9019 0.8737 0.8241 0.9433 0.8027 0.9419 0.8769 0.7319 0.7606 0.8994 0.7324 0.9792 0.9183 0.8567 0.9121 0.9529
Cytoplasmic Foci 0.0425 0.004 0.0105 0.0317 0.027 0.0127 0.0248 0.0064 0.0219 0.09 0.0397 0.0126 0.0236 0.0027 0.0151 0.0246 0.0106 0.006
Eisosomes 0.0003 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0008 0 0.0002 0.0003 0.0001 0
Endoplasmic Reticulum 0.0134 0.0039 0.0037 0.0031 0.0022 0.0022 0.0098 0.0022 0.0025 0.0031 0.0014 0.0044 0.0157 0.0022 0.0027 0.0048 0.0066 0.0028
Endosome 0.058 0.0025 0.0146 0.023 0.0541 0.0104 0.0347 0.005 0.0112 0.0222 0.0191 0.0184 0.0196 0.0012 0.0059 0.0179 0.0229 0.0132
Golgi 0.0202 0.0002 0.0034 0.0092 0.0311 0.0016 0.007 0.0003 0.0039 0.0097 0.0054 0.0049 0.0069 0.0002 0.0021 0.0051 0.0092 0.0014
Lipid Particles 0.0125 0 0.0027 0.0028 0.0062 0.0004 0.0049 0.0001 0.0008 0.0189 0.0185 0.0038 0.014 0 0.004 0.0086 0.0064 0.0009
Mitochondria 0.0154 0.0003 0.0057 0.0028 0.0104 0.0049 0.0018 0.0001 0.0009 0.0017 0.0016 0.0116 0.0022 0.0001 0.0003 0.0024 0.0018 0.0003
None 0.0347 0.0168 0.0176 0.0124 0.0082 0.0102 0.0623 0.0398 0.037 0.0884 0.0469 0.0266 0.0616 0.0059 0.0101 0.0176 0.0021 0.0028
Nuclear Periphery 0.0123 0.0009 0.0013 0.0018 0.0011 0.0007 0.0062 0.0005 0.0007 0.0006 0.0006 0.0006 0.0107 0.0004 0.0013 0.002 0.0033 0.002
Nucleolus 0.002 0.0002 0.0012 0.0005 0.0001 0.0004 0.0008 0 0.0001 0.0001 0.0002 0 0.0012 0 0.0012 0.0008 0.001 0
Nucleus 0.0084 0.0019 0.0024 0.0029 0.0012 0.0023 0.011 0.0007 0.0012 0.001 0.001 0.0009 0.0063 0.0007 0.0019 0.0021 0.0025 0.0009
Peroxisomes 0.0137 0 0.0062 0.0077 0.0115 0.0007 0.0052 0 0.0049 0.0242 0.0431 0.0034 0.012 0 0.0059 0.0053 0.001 0.0003
Punctate Nuclear 0.0052 0.0012 0.0031 0.0068 0.0026 0.0006 0.0077 0.0001 0.0004 0.0031 0.0108 0.0004 0.0071 0.0002 0.0052 0.003 0.0034 0.0013
Vacuole 0.0051 0.0012 0.0052 0.0033 0.003 0.0017 0.0061 0.0016 0.0018 0.0016 0.0024 0.0012 0.0025 0.0005 0.001 0.0054 0.0022 0.0005
Vacuole Periphery 0.0019 0.0002 0.0013 0.0008 0.0018 0.0007 0.0014 0.0002 0.0003 0.0004 0.0003 0.0004 0.001 0.0001 0.0002 0.0006 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.7117 17.9808 22.1105 32.2434 30.9221 16.0824 25.1928 27.8716 25.8035 32.9671
Translational Efficiency 1.6621 2.4333 1.8374 1.0035 1.3872 2.3084 1.4487 1.2086 1.5047 1.3776

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RING finger ubiquitin ligase (E3); involved in ubiquitination and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hel2

Hel2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hel2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available