Standard name
Human Ortholog
Description Diacylglycerol pyrophosphate (DGPP) phosphatase; zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.25 0.07 0.35 0.17 0.24 0.22 0.23 – 0.25 0.32 0.28 0.18 0.19 0.17 0.13 0.17 0.12 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0.05 0.1 0.1 0.08 – 0.16 0.12 0.11 0.05 0.05 0 0 0 0 0.07 0 0 0 0 0
Endosome – 0 0.13 0.19 0 0 0 0 – 0 0 0 0.05 0.1 0.11 0.08 0.13 0.1 0.14 0.18 0.18 0.13 0.16 0.15
Golgi – 0 0 0 0.11 0 0 0 – 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0
Mitochondria – 0.06 0.25 0.15 0.22 0.32 0.43 0.41 – 0.28 0.38 0.4 0 0 0 0 0 0 0 0 0.06 0.05 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.7 0.71 0.56 0.59 0.43 0.47 0.39 – 0.42 0.34 0.35 0.79 0.75 0.76 0.85 0.81 0.85 0.52 0.68 0.66 0.73 0.71 0.72
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 2 2 – 6 0 6 2 5 1 0 0 0 0 0 1 1 1 2
Bud – 0 0 0 0 3 2 6 – 1 1 1 0 0 1 0 1 0 0 4 1 2 4 4
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 1 3 4 – 9 5 12 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 57 18 49 43 64 74 90 – 102 97 122 69 89 78 26 40 19 1 3 4 5 3 2
Endoplasmic Reticulum – 8 2 1 12 28 33 31 – 67 36 46 19 22 19 4 9 2 2 3 0 5 2 3
Endosome – 8 32 27 8 2 2 8 – 7 0 4 19 49 51 15 31 15 5 45 49 45 69 60
Golgi – 3 1 3 28 10 5 7 – 14 8 13 7 13 12 5 12 9 2 4 5 4 4 5
Mitochondria – 13 64 21 57 87 143 161 – 114 117 174 0 1 1 6 8 0 1 10 16 17 14 13
Nucleus – 6 2 0 0 2 3 1 – 2 1 0 3 3 4 3 0 2 0 2 0 3 3 4
Nuclear Periphery – 2 8 0 4 14 9 6 – 13 7 12 3 2 4 3 1 2 0 1 8 8 11 10
Nucleolus – 1 2 0 0 1 1 2 – 0 0 2 0 0 1 0 2 1 0 0 0 0 1 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 1 2 0 0 1 0 0 – 0 0 0 1 2 2 0 0 0 0 3 0 1 4 1
Vac/Vac Membrane – 161 179 78 152 117 158 152 – 170 105 153 299 363 343 164 186 134 20 170 177 260 299 285
Unique Cell Count 231 251 140 257 269 335 390 408 305 434 377 481 453 193 231 157 40 252 269 359 423 396
Labelled Cell Count 260 310 179 304 330 435 470 505 377 545 422 549 517 226 290 184 40 252 269 359 423 396


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 7.3 6.2 7.7 8.2 8.1 7.9 8.3 – 9.1 8.9 9.2 8.9 8.7 9.0 10.1 9.9 10.9 8.8 9.2 9.6
Std Deviation (1e-4) – 1.8 1.6 2.5 1.9 1.8 2.1 2.0 – 2.0 2.0 2.0 2.3 2.3 2.3 2.3 2.5 2.6 2.1 2.4 2.6
Intensity Change (Log2) – – – 0.33 0.41 0.39 0.36 0.43 – 0.56 0.53 0.58 0.54 0.5 0.54 0.71 0.68 0.83 0.52 0.58 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 7.0 3.3 5.2 4.9 5.3 – 5.8 7.1 6.5 4.0 4.1 3.7 2.2 3.4 1.7
Endoplasmic Reticulum 0 2.7 4.7 4.6 4.0 – 6.4 5.1 4.8 2.9 2.7 2.5 0 2.3 0
Endosome 1.7 -4.0 -5.5 -6.2 -5.5 – -5.8 -6.4 -6.7 -3.5 -1.0 -0.6 -1.7 0.2 -1.0
Golgi 0 5.1 2.6 0 0 – 2.5 0 2.3 0 0 0 0 3.2 0
Mitochondria -2.4 -0.9 1.7 4.3 4.1 – 0.7 3.2 3.9 -10.3 -11.4 -11.1 -6.4 -6.8 -6.9
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 -1.2 1.1 -0.4 -1.4 – 0 -0.6 -0.3 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -3.1 -2.9 -6.4 -5.9 -8.0 – -7.4 -8.7 -9.1 2.3 1.2 1.3 3.4 2.4 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.993 3.5779 2.9942 2.6619 3.034 3.1395 5.7829 5.4576 5.6152 5.028 5.454 5.7551 2.6438 3.7799 3.1342 3.8879 2.3772 3.1508
Actin 0.0072 0.0001 0.0018 0.0263 0.0143 0.0015 0.0209 0.0015 0.0103 0.0011 0.0072 0.0028 0.0144 0 0.0001 0.0101 0 0
Bud 0.0003 0.0017 0.0012 0.0043 0.0003 0.0012 0.0005 0.0073 0.0056 0.0058 0.0018 0.0006 0.0011 0.0003 0.0004 0.0002 0.0007 0.0002
Bud Neck 0.0006 0.0003 0.0005 0.0017 0.0005 0.0003 0.0007 0.0004 0.0017 0.0007 0.0005 0.0003 0.0004 0.0002 0.0003 0.0006 0.0004 0.0001
Bud Periphery 0.0006 0.0021 0.0018 0.012 0.0005 0.0011 0.0011 0.0108 0.0107 0.0093 0.0037 0.0011 0.0017 0.0004 0.0008 0.0004 0.0009 0.0006
Bud Site 0.0012 0.0014 0.0022 0.0026 0.0003 0.0002 0.0012 0.0041 0.0029 0.0032 0.0015 0.0002 0.0005 0.0013 0.0007 0.0005 0.0006 0.0001
Cell Periphery 0.0005 0.0004 0.0005 0.0004 0.0003 0.0003 0.0007 0.0005 0.0006 0.0003 0.0003 0.0002 0.0006 0.0004 0.0005 0.0002 0.0002 0.0002
Cytoplasm 0.0097 0.0214 0.0199 0.0331 0.0288 0.0082 0.0133 0.074 0.0192 0.0136 0.0179 0.0142 0.01 0.0133 0.0144 0.0036 0.0168 0.0132
Cytoplasmic Foci 0.0294 0.0133 0.0221 0.013 0.0191 0.008 0.0213 0.0215 0.0225 0.0282 0.0151 0.0146 0.0127 0.0092 0.015 0.0066 0.0101 0.0102
Eisosomes 0.0001 0 0.0001 0.0002 0.0001 0 0.0003 0 0.0002 0 0.0001 0 0.0002 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0055 0.0039 0.0038 0.002 0.0056 0.0039 0.0089 0.0059 0.0074 0.0097 0.0044 0.0034 0.0052 0.0037 0.0064 0.005 0.0016 0.0035
Endosome 0.3183 0.2199 0.2897 0.3153 0.2931 0.2436 0.3049 0.2444 0.3061 0.3361 0.2813 0.3296 0.2727 0.2032 0.3154 0.2364 0.2737 0.2944
Golgi 0.0179 0.0081 0.0113 0.0271 0.035 0.0081 0.023 0.0124 0.0152 0.0232 0.0221 0.0142 0.011 0.0057 0.0184 0.0254 0.0084 0.0135
Lipid Particles 0.0214 0.0029 0.01 0.0073 0.0145 0.0044 0.0127 0.0017 0.0088 0.0038 0.0413 0.0039 0.0176 0.0025 0.0143 0.0041 0.0088 0.0039
Mitochondria 0.0114 0.0111 0.0187 0.014 0.0118 0.0157 0.0273 0.0191 0.0387 0.031 0.0311 0.0236 0.0098 0.005 0.0204 0.0326 0.008 0.0088
None 0.0078 0.0141 0.0365 0.0046 0.0181 0.0128 0.0121 0.0496 0.0396 0.0036 0.0084 0.0067 0.0207 0.0014 0.0047 0.0535 0.0004 0.0152
Nuclear Periphery 0.0168 0.0202 0.0126 0.0041 0.0127 0.0286 0.0182 0.0288 0.0093 0.0111 0.0212 0.0155 0.0218 0.019 0.0229 0.0075 0.0216 0.0194
Nucleolus 0.0021 0.0012 0.0012 0.0004 0.0005 0.0006 0.0019 0.0013 0.0019 0.0024 0.0019 0.0004 0.0013 0.0006 0.0005 0.0003 0.0004 0.0003
Nucleus 0.0055 0.0051 0.0034 0.0014 0.0014 0.002 0.0033 0.0044 0.0027 0.0039 0.0043 0.0013 0.0042 0.0022 0.0024 0.0006 0.0009 0.0014
Peroxisomes 0.0074 0.0003 0.0035 0.009 0.0017 0.0004 0.0047 0.0042 0.0041 0.0048 0.005 0.0016 0.0012 0.0002 0.0007 0.0006 0.0002 0.0002
Punctate Nuclear 0.0105 0.0002 0.0012 0.0003 0.0004 0.0006 0.001 0.0008 0.0009 0.0007 0.0033 0.0005 0.0017 0.0001 0.0002 0.0003 0.0001 0.0001
Vacuole 0.3016 0.3926 0.2942 0.2915 0.2444 0.2763 0.259 0.2692 0.244 0.2425 0.1641 0.2218 0.3194 0.3694 0.281 0.2719 0.2442 0.2094
Vacuole Periphery 0.224 0.2797 0.2639 0.2293 0.2965 0.3822 0.2629 0.2382 0.2475 0.265 0.3633 0.3434 0.2719 0.3617 0.2804 0.3394 0.4021 0.4054

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.6826 28.3441 26.9217 34.3541 27.1323 27.8867 33.2441 37.138 39.9831 40.1872
Translational Efficiency 1.1824 1.2445 1.0317 0.7658 1.2386 1.6502 1.1947 0.801 0.9622 1.0464

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Diacylglycerol pyrophosphate (DGPP) phosphatase; zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism
Localization
Cell Percentages vacuolar membrane (46%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Dpp1

Dpp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dpp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available