Standard name
Human Ortholog
Description Dihydrosphingosine phosphate lyase; regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0.07 0.06 – 0 0 0 0.09 – – – 0.08 0.08 0.13 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0.07 0 – 0 0.05 0.07 0.09 – – – 0.3 0.34 0.32 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0.25 0.26 0.17 0.17 – 0.2 0.25 0.24 0.16 – – – 0.13 0.11 0.21 0 0 0 0 0.07 0.14
Endoplasmic Reticulum 0.96 0.9 0.91 0.71 0.6 0.65 0.64 – 0.49 0.49 0.47 0.6 – – – 0 0 0 0.92 0.93 0.92 0.67 0.42 0.41
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.08 0.05 0.08
Golgi 0 0 0 0 0.05 0 0 – 0 0 0 0 – – – 0.42 0.44 0.29 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0 – 0.12 0.09 0.2 0.12 – – – 0.09 0 0 0 0 0 0 0.08 0.06
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.1 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0.17 0.12 0.19 0.14 0.18 – 0.19 0.23 0.12 0.12 – – – 0.22 0.17 0.16 0 0 0 0 0.26 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 9 5 6 5 16 18 – 3 11 9 23 – – – 20 15 20 2 2 0 0 0 1
Bud 0 0 0 0 1 0 3 – 0 1 4 1 – – – 1 0 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 11 3 3 9 17 12 – 6 12 15 23 – – – 73 62 49 0 1 0 0 0 0
Cytoplasm 2 17 3 34 64 42 55 – 37 64 49 39 – – – 31 20 31 0 4 0 0 1 6
Endoplasmic Reticulum 43 205 82 96 148 161 201 – 91 126 95 146 – – – 3 3 2 79 225 102 6 10 19
Endosome 0 0 0 0 1 0 3 – 3 0 1 0 – – – 0 0 0 0 1 0 0 1 3
Golgi 1 5 2 2 12 9 2 – 1 1 0 3 – – – 103 82 44 1 0 0 0 0 0
Mitochondria 1 0 0 0 1 20 11 – 23 23 41 28 – – – 22 5 2 0 1 2 0 2 3
Nucleus 0 1 1 1 3 0 5 – 3 3 4 3 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 1 0 1 3 5 7 – 4 5 5 9 – – – 1 2 1 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 1 0 1 1 – – – 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 1 12 15 17 47 35 57 – 35 59 24 30 – – – 54 31 24 0 3 1 0 6 5
Unique Cell Count 45 228 90 136 248 246 315 186 255 204 243 245 185 151 86 243 111 10 25 49
Labelled Cell Count 50 261 111 160 294 305 374 207 305 248 306 308 221 175 86 243 111 10 25 49


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 15.5 12.7 12.2 12.8 11.2 10.9 – 9.3 9.9 9.1 9.1 – – – 21.4 21.9 20.7 – – –
Std Deviation (1e-4) 1.6 2.2 1.7 3.0 3.1 2.3 2.1 – 1.8 2.2 1.8 1.8 – – – 4.4 4.9 5.0 – – –
Intensity Change (Log2) – – – -0.06 0.01 -0.19 -0.22 – -0.46 -0.37 -0.48 -0.48 – – – 0.75 0.78 0.7 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0.3 0.1 – 0 0 0 1.1 – – – 0.8 0.8 1.9
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 1.2 0 – 0 0 1.3 1.9 – – – 5.1 5.5 5.3
Cytoplasm 4.3 4.6 3.3 3.4 – 3.7 4.5 4.3 3.1 – – – 2.5 2.1 3.7
Endoplasmic Reticulum -3.7 -5.5 -4.7 -5.0 – -6.8 -7.0 -7.2 -5.4 – – – -16.8 -15.1 -14.1
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 7.0 7.1 5.1
Mitochondria 0 0 2.8 0 – 3.5 2.9 4.6 3.4 – – – 2.9 0 0
Nucleus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole -0.9 0.5 -0.6 0.3 – 0.4 1.3 -1.1 -1.0 – – – 1.1 0 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.0053 7.8324 6.7007 7.3099 6.0496 7.5123 7.3081 9.2625 8.0064 7.1745 7.5803 8.3455 7.4092 8.4672 7.6031 7.2662 7.6961 8.2234
Actin 0.0152 0.0007 0.0341 0.0072 0.007 0.0003 0.0269 0.0024 0.0022 0.0358 0.0015 0.0011 0.032 0.0007 0.0087 0.0512 0.0002 0.0088
Bud 0.0001 0 0.0009 0.0088 0.0007 0 0.0002 0.0001 0.0001 0.0007 0.0001 0 0.0003 0.0002 0.0005 0.0005 0 0.0028
Bud Neck 0.0003 0.0001 0.0024 0.001 0.0008 0.0001 0.0003 0.0002 0.0001 0.0011 0.0006 0.0002 0.0004 0.0002 0.0005 0.0018 0.0003 0.0007
Bud Periphery 0.0002 0 0.001 0.0117 0.0014 0 0.0003 0.0002 0.0002 0.0007 0.0005 0.0001 0.0003 0.0001 0.0003 0.0006 0.0001 0.0064
Bud Site 0.0006 0.0001 0.0081 0.0017 0.0031 0 0.0005 0.0003 0.0002 0.0107 0.0004 0.0001 0.0009 0.0004 0.0007 0.0035 0.0001 0.0017
Cell Periphery 0.0008 0.0009 0.0007 0.0007 0.0011 0.0001 0.0008 0.0013 0.0009 0.0008 0.0012 0.0003 0.0006 0.0026 0.0011 0.0008 0.0029 0.0004
Cytoplasm 0.013 0.0197 0.03 0.05 0.0173 0.0116 0.0144 0.0349 0.0088 0.0032 0.0155 0.0025 0.0067 0.0328 0.0252 0.0034 0.005 0.0151
Cytoplasmic Foci 0.0108 0.0014 0.0081 0.0019 0.0136 0.0027 0.0154 0.0034 0.0047 0.0149 0.0025 0.0072 0.0106 0.0123 0.0056 0.0432 0.0007 0.0011
Eisosomes 0.0003 0 0.0004 0.0001 0.0002 0 0.0006 0 0.0002 0.0003 0.0001 0 0.0004 0 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.8206 0.9412 0.8128 0.8496 0.7471 0.9569 0.7906 0.9219 0.9268 0.8359 0.8574 0.9473 0.8236 0.9115 0.8893 0.7883 0.9225 0.936
Endosome 0.032 0.007 0.0204 0.0069 0.0497 0.0055 0.0301 0.0078 0.0104 0.0141 0.0394 0.0114 0.0155 0.0018 0.0113 0.0204 0.0262 0.0065
Golgi 0.0117 0.0057 0.0083 0.0007 0.0149 0.0012 0.0157 0.0045 0.0041 0.0224 0.0032 0.005 0.0106 0.0004 0.0073 0.025 0.0033 0.0049
Lipid Particles 0.0113 0.0029 0.0093 0.0009 0.0355 0.0013 0.0241 0.0084 0.0097 0.0161 0.0053 0.0039 0.0162 0.0049 0.0103 0.0107 0.0078 0.0026
Mitochondria 0.0022 0.0019 0.0075 0.0009 0.0211 0.0003 0.006 0.0015 0.0078 0.0153 0.025 0.002 0.0114 0.0005 0.0054 0.0051 0.0012 0.0036
None 0.0024 0.0002 0.0039 0.0226 0.0026 0.0001 0.0017 0.0001 0.0004 0.0005 0.0014 0.0002 0.0022 0.0024 0.0042 0.001 0.0002 0.0001
Nuclear Periphery 0.0508 0.0121 0.0201 0.0182 0.0451 0.014 0.0311 0.0081 0.0131 0.0069 0.0245 0.0108 0.0438 0.0136 0.009 0.0046 0.0045 0.0053
Nucleolus 0.0002 0 0.0015 0.0006 0.0011 0 0.0003 0 0.0001 0.0001 0.0005 0 0.0007 0.0011 0.0003 0.0007 0.0003 0.0001
Nucleus 0.0026 0.0016 0.0091 0.0075 0.0049 0.0007 0.0012 0.0005 0.0043 0.0005 0.0044 0.0006 0.0049 0.0016 0.0018 0.0006 0.0005 0.0006
Peroxisomes 0.0138 0.0003 0.0099 0.0002 0.0083 0.0029 0.0167 0.0006 0.003 0.0166 0.0003 0.0028 0.0107 0.0001 0.0123 0.0115 0.0001 0.0006
Punctate Nuclear 0.0015 0.0001 0.0051 0.0049 0.0089 0.0001 0.0162 0.0004 0.0005 0.0009 0.0041 0.0021 0.0035 0.0051 0.0028 0.0249 0.0001 0.0003
Vacuole 0.0061 0.0035 0.0036 0.0029 0.0085 0.0018 0.0045 0.0027 0.0018 0.002 0.0074 0.0019 0.0028 0.0069 0.0025 0.0015 0.0206 0.0018
Vacuole Periphery 0.0033 0.0004 0.0029 0.0013 0.0073 0.0004 0.0023 0.0003 0.0007 0.0006 0.0046 0.0004 0.0018 0.0003 0.0007 0.0004 0.0035 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.3521 58.5138 40.5251 50.1256 35.5632 45.4824 57.6138 64.6506 55.4841 52.1665
Translational Efficiency 0.8488 0.7733 1.0019 0.7952 0.9628 0.889 0.787 0.6191 0.7398 0.6921

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dihydrosphingosine phosphate lyase; regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Dpl1

Dpl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dpl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available