Standard name
Human Ortholog
Description Sphinganine C4-hydroxylase; catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0.07 0.05 0 0 0 0 0 0 0 0 0 0.08 0.07 0.12 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.06 0.05 0.05 0.05 0 0.05 0.05 0.06 0.09 0.1 0.21 0.41 0.35 0.32 0 0 0 0 0 0
Cytoplasm 0.13 0.16 0.07 0.14 0.14 0.18 0.15 0.2 0.23 0.22 0.19 0.09 0.08 0.09 0.22 0.27 0.28 0 0 0 0 0 0
Endoplasmic Reticulum 0.61 0.81 0.87 0.52 0.49 0.55 0.35 0.26 0.31 0.16 0.34 0.86 0.83 0.76 0 0 0 0.92 0.88 0.9 0.85 0.84 0.84
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.18 0.19 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.22 0.12 0.17 0.28 0.21 0.2 0.34 0.33 0 0 0 0.09 0.11 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.15 0.06 0 0 0.05 0 0.05 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0 0.05 0.15 0.3 0.18 0.32 0.37 0.3 0.31 0.19 0.09 0.05 0.07 0.07 0.09 0.14 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 14 18 21 15 20 23 23 13 28 8 9 10 8 7 12 0 2 4 3 2 4
Bud 0 0 0 2 7 14 6 19 13 14 23 1 2 2 0 0 1 0 1 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cell Periphery 6 2 6 17 19 21 27 17 26 22 43 21 24 51 43 33 32 0 1 1 0 2 4
Cytoplasm 20 11 17 36 58 75 81 117 124 102 131 21 20 22 23 26 28 1 2 1 0 6 5
Endoplasmic Reticulum 97 54 198 138 203 228 198 149 168 72 239 192 210 184 2 1 3 228 82 232 161 195 227
Endosome 1 0 0 5 10 1 5 7 6 8 4 0 1 1 0 0 0 1 0 0 1 0 2
Golgi 5 0 1 6 7 1 3 0 3 2 0 1 6 1 24 17 19 0 0 1 0 2 2
Mitochondria 4 1 18 59 52 69 159 121 109 156 234 0 0 1 9 10 6 2 0 7 4 2 4
Nucleus 1 0 1 0 4 3 6 7 7 4 2 1 3 3 1 0 0 0 0 0 0 0 0
Nuclear Periphery 5 1 35 15 13 12 28 20 25 6 39 4 3 5 0 5 1 7 1 6 5 3 2
Nucleolus 2 0 0 1 0 0 2 1 0 5 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 43 3 11 40 124 77 178 216 165 141 135 19 13 17 7 9 14 2 0 1 8 7 11
Unique Cell Count 159 67 228 263 418 418 558 578 546 461 703 222 252 241 105 95 101 249 94 258 190 232 272
Labelled Cell Count 185 73 301 337 518 516 713 698 672 545 880 268 291 298 117 108 116 249 94 258 190 232 272


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.7 14.1 9.5 10.2 10.9 10.4 9.5 9.7 9.0 9.1 8.8 14.5 13.2 12.7 19.0 20.0 20.4 14.5 14.9 14.4
Std Deviation (1e-4) 6.2 4.0 2.4 2.4 2.6 2.4 2.4 2.1 2.3 2.2 2.0 3.0 3.3 2.9 4.6 4.5 5.4 3.1 3.4 3.2
Intensity Change (Log2) 0.09 0.19 0.12 -0.0 0.03 -0.09 -0.08 -0.12 0.6 0.47 0.41 0.99 1.07 1.1 0.6 0.65 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 -0.6 -1.5 -1.6 -1.3 -1.1 -2.1 -1.4 -1.2 -1.3 -1.0 0.5 0.4 1.8
Bud 0 0 0 0 2.8 0 0 2.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.0 1.2 1.5 1.4 0.2 1.4 1.3 2.0 3.0 3.1 6.1 9.2 8.1 7.6
Cytoplasm 2.2 2.4 3.6 2.7 4.4 5.0 4.8 4.0 0.8 0.2 0.7 3.8 4.8 4.9
Endoplasmic Reticulum -8.2 -9.6 -8.3 -13.1 -15.8 -14.2 -18.0 -13.9 -0.1 -1.1 -2.9 -14.7 -14.4 -14.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 7.2 6.2 6.4
Mitochondria 4.4 1.8 3.1 6.3 4.4 4.1 7.4 7.5 -4.3 -4.5 -4.1 0.2 0.8 -0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -3.5 -5.7 -5.8 -4.8 -6.0 -5.1 -7.3 -4.8 -5.1 -5.7 -5.1 -4.2 -2.5 -3.8
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.8 7.4 4.8 8.1 9.3 7.7 7.7 5.2 1.6 0.2 1.0 0.7 1.6 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.1233 8.7279 9.0223 6.0302 7.7175 8.0239 10.1247 12.9079 12.0196 10.6782 8.9904 11.0424 7.8062 9.327 10.0423 8.2171 8.3668 8.9623
Actin 0.0092 0.001 0.0104 0.004 0.003 0.0013 0.0118 0.0003 0.0025 0.0002 0.001 0.0001 0.0076 0.0001 0.0001 0.002 0.0045 0.0017
Bud 0.0003 0.0001 0.0003 0.0027 0.0002 0.0005 0.0001 0 0.0001 0 0.0001 0 0.0004 0 0 0 0.0003 0
Bud Neck 0.0005 0.0011 0.0003 0.0002 0.0008 0.0011 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0006 0.0004 0.0001 0.0001 0.0003 0.0016
Bud Periphery 0.0005 0.0001 0.0009 0.0036 0.0004 0.0004 0.0002 0.0002 0.0001 0.0001 0.0007 0 0.0009 0 0 0 0.0005 0.0001
Bud Site 0.0057 0.0038 0.0012 0.0007 0.0016 0.0002 0.0006 0.0007 0.0053 0.0001 0.0002 0 0.0041 0.0006 0 0.0002 0.0005 0
Cell Periphery 0.0009 0.0039 0.0028 0.0009 0.001 0.0034 0.0007 0.0025 0.0017 0.0032 0.0018 0.0003 0.0011 0.0012 0.0004 0.0002 0.0003 0.0005
Cytoplasm 0.0249 0.0674 0.0249 0.0115 0.0173 0.0523 0.0122 0.0129 0.0094 0.0026 0.0157 0.0057 0.0116 0.0302 0.0052 0.0094 0.0274 0.0115
Cytoplasmic Foci 0.0105 0.002 0.0074 0.0082 0.0106 0.0077 0.0064 0.0007 0.0035 0.0034 0.0027 0.0003 0.0039 0.001 0.0002 0.0114 0.004 0.0003
Eisosomes 0.0004 0 0.0001 0 0.0002 0 0.0004 0 0 0 0.0001 0 0.0004 0 0 0 0.0002 0
Endoplasmic Reticulum 0.8089 0.8643 0.8804 0.7969 0.7663 0.8549 0.8724 0.947 0.9374 0.93 0.8916 0.9755 0.8831 0.9449 0.9856 0.9259 0.801 0.9657
Endosome 0.0295 0.0102 0.0177 0.0453 0.0779 0.0181 0.0218 0.0045 0.0083 0.029 0.0182 0.0017 0.0103 0.0023 0.0013 0.0116 0.0435 0.0019
Golgi 0.0093 0.0019 0.003 0.0169 0.0104 0.0066 0.007 0.0005 0.0066 0.0042 0.0151 0.0003 0.0031 0.0002 0.0003 0.0039 0.0139 0.0008
Lipid Particles 0.0108 0.0089 0.009 0.0059 0.0227 0.0079 0.0164 0.0072 0.0053 0.0062 0.018 0.0017 0.0044 0.0036 0.0012 0.0127 0.0236 0.0014
Mitochondria 0.0152 0.0009 0.0074 0.0568 0.0047 0.0045 0.0057 0.0003 0.0054 0.0007 0.012 0.0001 0.002 0.0001 0 0.0006 0.0509 0.0001
None 0.003 0.0005 0.0059 0.0012 0.0037 0.0017 0.0036 0.0002 0.0003 0.0002 0.0018 0.0006 0.0098 0.0001 0 0.0005 0.001 0.0001
Nuclear Periphery 0.0458 0.0226 0.0166 0.022 0.0459 0.0211 0.0258 0.0148 0.0049 0.005 0.0104 0.011 0.0375 0.0101 0.0041 0.0138 0.0131 0.0108
Nucleolus 0.0011 0.0001 0.0001 0.0001 0.0021 0.0003 0.0002 0 0 0.0001 0.0001 0 0.0005 0 0 0 0.0003 0
Nucleus 0.0111 0.0025 0.0017 0.0024 0.0052 0.0045 0.0023 0.0013 0.0003 0.0003 0.0013 0.0004 0.0026 0.001 0.0001 0.0013 0.0037 0.001
Peroxisomes 0.0039 0.0001 0.0006 0.0023 0.0032 0.0015 0.0032 0 0.0042 0.0002 0.0003 0 0.0058 0 0 0.0022 0.0025 0
Punctate Nuclear 0.0027 0.0004 0.0017 0.0048 0.0055 0.0042 0.0043 0.0002 0.0001 0.0001 0.0004 0.0002 0.0008 0.0003 0 0.001 0.0021 0.0001
Vacuole 0.004 0.0076 0.0066 0.0098 0.0125 0.0063 0.003 0.0053 0.0037 0.0133 0.0062 0.0018 0.0078 0.0035 0.0012 0.0029 0.0046 0.0022
Vacuole Periphery 0.0021 0.0007 0.0011 0.0039 0.0048 0.0014 0.0016 0.0012 0.0006 0.001 0.0021 0.0003 0.0016 0.0003 0.0001 0.0005 0.0017 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 157.1038 101.4558 50.8868 55.5134 115.8115 115.5308 88.8093 56.7349 61.9074 120.7888
Translational Efficiency 1.1624 1.2535 1.2121 1.2886 1.2819 1.1302 0.9672 1.0703 0.9295 0.9936

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1273 1216 733 1135 1504 776 1702 1478 2777 1992 2435 2613

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1218.35 1243.59 1615.63 2149.55 1198.33 1177.24 1701.67 1876.13 1207.51 1217.74 1675.77 1994.89
Standard Deviation 221.61 271.03 263.14 455.02 289.58 238.10 307.43 399.48 260.82 260.72 297.43 445.61
Intensity Change Log 2 0.029582 0.407168 0.819106 -0.025617 0.505926 0.646735 0.002475 0.456983 0.736206

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000610 0.001962 0.000939 0.000899 0.001175 0.001318 0.001328 0.000797 0.000916 0.001711 0.001211 0.000841
Bud Neck 0.000881 0.000413 0.002900 0.002452 0.000414 0.000556 0.000847 0.001628 0.000628 0.000469 0.001466 0.001986
Bud Site 0.004048 0.005281 0.005069 0.008193 0.005793 0.007193 0.003529 0.007941 0.004993 0.006026 0.003993 0.008051
Cell Periphery 0.001858 0.001791 0.002445 0.018631 0.002345 0.003260 0.003014 0.010929 0.002121 0.002363 0.002843 0.014274
Cytoplasm 0.004145 0.013814 0.010629 0.004674 0.007544 0.012621 0.002363 0.004083 0.005986 0.013349 0.004851 0.004340
Cytoplasmic Foci 0.002123 0.005717 0.000906 0.001812 0.011276 0.005242 0.000379 0.001633 0.007080 0.005532 0.000537 0.001710
Eisosomes 0.000019 0.000035 0.000041 0.000204 0.000032 0.000076 0.000070 0.000106 0.000026 0.000051 0.000061 0.000149
Endoplasmic Reticulum 0.964995* 0.898647* 0.884916* 0.885663* 0.908128* 0.928044* 0.972344* 0.931542* 0.934196* 0.910099* 0.946026* 0.911613*
Endosome 0.004673 0.017466 0.012514 0.009465 0.015198 0.009407 0.003821 0.007004 0.010373 0.014326 0.006438 0.008073
Golgi 0.001469 0.006132 0.000885 0.008379 0.008178 0.003779 0.000622 0.004456 0.005103 0.005215 0.000701 0.006160
Lipid Particles 0.001050 0.009454 0.008355 0.018024 0.005253 0.003731 0.002562 0.009013 0.003326 0.007224 0.004306 0.012927
Mitochondria 0.001230 0.001233 0.000178 0.001535 0.001803 0.002894 0.000215 0.001345 0.001540 0.001880 0.000204 0.001427
Mitotic Spindle 0.000113 0.000152 0.000503 0.001664 0.000170 0.000391 0.000116 0.000794 0.000144 0.000246 0.000232 0.001172
None 0.001576 0.003871 0.000186 0.001875 0.002743 0.000945 0.000181 0.001030 0.002208 0.002731 0.000183 0.001397
Nuclear Periphery 0.000905 0.003164 0.018723 0.002888 0.002260 0.001442 0.001235 0.000955 0.001639 0.002493 0.006499 0.001795
Nuclear Periphery Foci 0.004379 0.019302 0.037444 0.010739 0.013227 0.008719 0.003536 0.003928 0.009171 0.015179 0.013743 0.006886
Nucleolus 0.000111 0.000095 0.000009 0.000024 0.000176 0.000045 0.000003 0.000015 0.000147 0.000075 0.000004 0.000018
Nucleus 0.001730 0.000610 0.001002 0.000476 0.001150 0.000961 0.000281 0.000294 0.001416 0.000747 0.000498 0.000373
Peroxisomes 0.000127 0.000398 0.000018 0.000043 0.000446 0.000367 0.000048 0.000156 0.000300 0.000386 0.000039 0.000107
Vacuole 0.003153 0.007925 0.011863 0.018781 0.008007 0.005756 0.003259 0.009699 0.005782 0.007080 0.005849 0.013644
Vacuole Periphery 0.000805 0.002539 0.000475 0.003579 0.004679 0.003255 0.000248 0.002655 0.002904 0.002818 0.000316 0.003056

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.75 -0.99 -1.34 1.12 -0.01 -0.25 -0.35 0.65 0.94 1.02 -1.48 -0.91 -0.20 1.46 0.86
Bud Neck 1.92 -3.44 -3.45 -4.97 0.78 -1.09 -2.17 -3.92 -3.45 -2.21 1.30 -3.59 -5.14 -6.02 -1.55
Bud Site -0.89 -0.83 -2.60 -2.08 -1.52 -0.76 1.55 -1.80 -0.82 -3.05 -0.95 0.63 -3.08 -2.20 -3.52
Cell Periphery 0.22 -1.48 -8.20 -8.28 -7.86 -0.84 -1.03 -6.66 -4.79 -5.63 -0.48 -1.55 -10.55 -9.91 -9.35
Cytoplasm -5.18 -3.91 -0.82 3.98 2.92 -2.32 5.04 2.43 3.68 -1.79 -5.15 0.74 1.19 5.65 0.52
Cytoplasmic Foci -3.08 1.85 0.47 3.40 -1.30 3.42 7.67 6.34 3.03 -2.24 1.41 7.96 5.99 4.55 -2.81
Eisosomes -2.68 -2.32 -4.54 -4.15 -3.93 -2.44 -1.83 -2.96 -1.09 -1.01 -3.16 -2.34 -5.40 -4.17 -3.11
Endoplasmic Reticulum 9.38 9.80 10.36 2.01 -0.14 -2.35 -10.39 -2.66 0.03 7.54 4.29 -1.94 4.92 0.49 6.84
Endosome -10.63 -7.33 -6.01 6.20 2.79 4.29 11.57 7.34 1.91 -4.60 -4.17 5.39 2.92 6.83 -2.73
Golgi -4.57 1.26 -4.85 -1.36 -5.47 3.28 6.52 2.84 -0.77 -6.10 -0.13 6.60 -1.14 -1.03 -7.85
Lipid Particles -5.24 -4.55 -7.89 -3.33 -3.29 1.49 2.36 -2.77 -4.09 -4.66 -3.57 -1.52 -7.87 -3.92 -6.21
Mitochondria -0.01 2.44 -0.41 -0.44 -1.69 -1.14 5.24 0.72 1.45 -2.30 -0.74 5.18 0.10 0.65 -2.73
Mitotic Spindle -0.89 -2.67 -2.22 -2.17 -1.46 -1.78 0.57 -2.98 -1.84 -3.07 -1.82 -1.58 -3.22 -2.89 -2.77
None -1.94 3.07 -0.34 1.35 -1.87 2.02 3.02 1.65 -0.13 -1.40 -0.61 4.03 1.08 1.53 -2.33
Nuclear Periphery -3.11 -7.11 -6.10 0.26 6.27 1.88 2.47 3.43 1.66 1.29 -1.74 -6.03 -0.82 1.40 5.90
Nuclear Periphery Foci -7.13 -10.48 -4.07 3.38 7.93 2.12 5.96 5.30 2.62 -0.61 -3.74 -3.49 1.56 5.25 5.25
Nucleolus 0.34 2.80 2.38 1.61 -2.95 1.45 1.86 1.74 2.05 -5.86 1.29 2.60 2.35 2.08 -6.03
Nucleus 1.81 0.84 1.85 0.12 2.16 0.31 1.35 1.45 2.13 0.17 1.57 1.85 2.34 1.98 1.14
Peroxisomes -1.16 4.22 3.16 1.52 -2.33 0.46 2.46 1.72 2.70 -2.03 -0.53 2.93 2.09 1.89 -2.17
Vacuole -3.34 -5.90 -7.35 -5.14 -2.88 1.54 4.05 -1.53 -2.76 -4.86 -1.24 -0.56 -6.53 -5.34 -6.32
Vacuole Periphery -4.12 1.41 -4.06 -1.43 -4.59 1.27 6.17 2.33 0.56 -5.23 0.15 6.42 -0.37 -0.51 -7.00
Standard name
Human Ortholog
Description Sphinganine C4-hydroxylase; catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Sur2

Sur2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sur2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available